Abstract: A strain of dominant bacteria was isolated from a uranium mill tailing piles located in Shaoguan city，Guangdong province，China． The bacteria was identified as Bacillus thuringiensis ( named Bt) on the basis of its colony morphology， physiological and biochemical characteristics，as well as 16S rDNA sequence homology analysis． Its uranium tolerance and the effect of different conditions ( temperature，pH value，NaCl concentration) on its growth were evaluated through batch experiments in the presence of 5 mg /L U6 + liquid culture through analyzing the value of D600 nm ． The results of single factor growth character experiments indicated that the optimum temperature，pH and salinity range for growth were 30 ℃，pH = 7. 0， and 0. 5 ～ 50 g /L( NaCl) ，respectively． The strain could tolerate a uranium concentration up to 100 mg /L U6 + at 25% incubation amount，30 ℃，initial pH 4 ～ 10，and it could preserve high growth activities in the salinity less than 8%． The results indicated that Bt strain has high resistance to uranium and has potential in application for bioleaching of uranium and bioremediation of uranium-polluted soil．