细菌Chryseobaterium sp.溶藻方式及其溶藻胶囊制备条件研究

王金霞; 陈玉成; 詹玲玲; 罗乐

doi:10.13205/j.hjgc.201909017

细菌Chryseobaterium sp.溶藻方式及其溶藻胶囊制备条件研究

doi: 10.13205/j.hjgc.201909017

1. 重庆工程职业技术学院;
2. 西南大学资源环境学院

基金项目:

国家科技支撑计划(2015BAL04B01)

重庆市教育委员会科学技术研究计划青年项目(KJQN201803406)

详细信息

作者简介:
王金霞(1980-),女,博士,副教授,主要研究方向为生态修复理论与技术。42219423@qq.com

中图分类号: X172;X52
计量
- 文章访问数: 11
- HTML全文浏览量: 5
- PDF下载量: 0
- 被引次数: 0
出版历程
- 收稿日期: 2019-04-23
- 网络出版日期: 2023-11-24
- 刊出日期: 2019-09-30

摘要

摘要: 为探究细菌Chryseobaterium sp.的溶藻能力及方式,通过扫描电镜观察细菌Chryseobaterium sp.对藻Anabaena flosaquae的作用效果,进一步通过分离菌体和菌上清液的方法探讨其溶藻的作用机制。结果表明:细菌Chryseobaterium sp.具有很强的溶藻能力,其主要通过分泌溶藻活性物质进行间接溶藻。胶囊制备的优化条件为:SA、CaCl₂、EC和柠檬酸质量分数分别为3%、3%、3%和2%,CaCO₃与SA的质量分数比为4/5,交联时间为30 min,溶藻物质质量分数为16%,该条件下所制胶囊缓慢释放溶藻物质,具有长效的溶藻能力。揭示了细菌Chryseobaterium sp.主要通过分泌溶藻活性物质进行间接溶藻,所制胶囊具有较强的溶藻能力,为细菌Chryseobaterium sp.溶藻机理和生物控藻技术的研究提供了理论依据。
- 溶藻细菌 /
- Chryseobateriumsp. /
- Anabaenaflos-aquae /
- 细菌上清液 /
- 溶藻胶囊

HTML全文

参考文献(36)

[1]	Xuan H L,Dai X Z,Li J,et al. A Bacillus sp. strain with antagonistic activity against Fusarium graminearum kills Microcystis aeruginosa selectively[J]. Science of the Total Environment,2017,583:214-221.
[2]	Li S Y,Liu W Z,Gu S,et al. Spatio-temporal dynamics of nutrients in the upper Han River basin,China[J]. Journal of Hazardous Materials,2009,162(2):1340-1346.
[3]	Sun R,Sun P F,Zhang J H,et al. Microorganisms-based methods for harmful algal blooms control:a review[J]. Bioresource Technology,2018,248:12-20.
[4]	Liao C L,Liu X B,Liu R F,et al. Two novel algicidal isolates kill Chlorella pyrenoidosa by inhibiting their host antioxidase activities[J]. Applied Biochemistry and Biotechnology,2015,177(2):567-576.
[5]	裴海燕,胡文容,曲音波,等.一株溶藻细菌的分离鉴定及其溶藻特性[J].环境科学学报,2005,25(6):796-802.
[6]	吴刚,席宇,赵以军.溶藻细菌研究的最新进展[J].环境科学研究,2002,15(5):43-46.
[7]	焦彦凯,严小军,李小兵.溶藻细菌及溶藻化合物研究进展[J].工业微生物,2018,48(4):60-66.
[8]	刘萍,张吉强.固定化溶藻菌除藻效果研究[J].环境工程,2016,33(12):28-31.
[9]	3种载体固定化菌藻共生系统脱氮除磷效果的对比[J].环境工程学报,2013,7(9):3256-3262.
[10]	刘燕.海洋溶藻弧菌的培养优化及溶藻粉剂胶囊球工艺研究[D].深圳:深圳大学,2017.
[11]	Andersen T,Strand B L,Formo K,et al. Alginates as biomaterials in tissue engineering[J]. Carbohydrate Chemistry:Chemical and Biological Approaches,2011,37:227-258.
[12]	Orive G, Carcaboso A. M, Hernandez R. M, et al.Biocompatibility evaluation of different alginates and alginate-based microcapsules[J]. Biomacromolecules,2005,6:927-931.
[13]	Ching S H,Bansal N,Bhandari B. Alginate gel particles-A review of production techniques and physical properties[J]. Critical Reviews in Food Science and Nutrition,2017,57(6):1133-1152.
[14]	齐素芳,余煜棉,赖子尼,等.复合载体固定化硝化细菌去除水体中氨氮的研究[J].广东工业大学学报,2007,24(2):15-19.
[15]	Sun H Y,Zhang Y,Chen H R,et al. Isolation and characterization of the marine algicidal bacterium Pseudoalteromonas S1against the harmful alga Akashiwo sanguinea[J]. Marine Biology,2016,163(3):66-74.
[16]	Qianlong F,Hengfeng Z,Juan H,et al. Algae-lysing effect of algicidal bacteria on Microcystis in bloom water[J]. Meteorological&Environmental Research,2016,7(6):29-32.
[17]	Bowman J P. Bioactive compound synthetic capacity and ecological significance of marine bacterial genus Pseudoalteromonas[J].Marine Drugs,2007,5(4):220-241.
[18]	史顺玉.溶藻细菌对藻类的生理生态效应及作用机理研究[D].武汉:中国科学院水生生物研究生所,2006.
[19]	Henrichsen J,Blom J. Examination of fimbriation of some gramnegative rods with and without twitching and gliding motility[J].Acta Pathologica Microbiologica Scandinavica Section B Microbiology,1975,83(3):161-170.
[20]	Panova T T,Ivanova J. Influence of physiological factors on the lysis effect of cytophaga on the red microalga Rhodella reticulata[J]. Applied and Environmental Microbiology,2000,88(2):358-363.
[21]	Costerton J W,Irvin R T,Cheng K J. The bacterial glycocalyx in nature and disease[J]. Annual Reviews in Microbiology,1981,35(1):299-324.
[22]	Kim Y S,Son H J,Jeong S Y. Isolation of an algicide from a marine bacterium and its effects against the toxic dinoflagellate Alexandrium catenella and other harmful algal bloom species[J].Journal of Microbiology,2015,53(8):511-517.
[23]	Sameeh M Y,Mohamed A A,Elazzazy A M. Polyphenolic contents and antimicrobial activity of different extracts of Padina boryana Thivy and Enteromorpha sp. marine algae[J]. Journal of Applied Pharmaceutical Science,2016,6(9):87-92.
[24]	Yoshikawa K, Adachi K, Nishij M, et al.β-Cyanoalanine production by marine bacteria on cyanide-free medium and its specific inhibitory activity toward cyanobacter[J]. Applied and Environmental Microbiology,2000,66(2):718-722.
[25]	Imamura N,Motoike I,Shimada N,et al. An efficient screening approach for anti-Microcystis compounds based on knowledge of aquatic microbial ecosystem[J]. the Journal of Antibiotics,2001,54(7):582-587.
[26]	Mitsutani A,Yamasaki I,Kitaguchi H,et al. Analysis of algicidal proteins of a diatom-lytic marine bacterium Pseudoalteromonas sp.strain A25 by two-dimensional electrophoresis[J]. Phycological,2001,40(3):286-291.
[27]	Kato J,Amie J,Murata Y,et al. Development of a genetic transformation system for an alga-lysing bacterium[J]. Applied and environmental microbiology,1998,64(6):2061-2064.
[28]	Paul S,Jinnque R,Haim B G. Bacterial suppression of Chlorella by hydroxylamine production[J]. Water Research,1979,13(1):267-273.
[29]	Sakata T,Yoshikawa T,Nishitarumizu S. Algicidal activity and identification of an algicidal substance produced by marine Pseudomonas sp. C55a-2[J]. Fisheries Science,2011,77(3):397-402.
[30]	Kodani S,Imoto A,Mitsutani A. Isolation and identification of the antialgal compound,harmane(1-methyl-β-carboline),produced by the algicidal bacterium,Pseudomonas sp. K44-1[J]. Journal of Applied Phycology,2002,14(2):109-114.
[31]	熊振湖,孙翠珍,刘青春.不同载体固定化藻菌共生系统的脱氮除磷效果[J].环境科学与技术,2005,28(1):82-84.
[32]	Chen M,Hu Y,Zhou J,et al. Facile fabrication of tea tree oilloaded antibacterial microcapsules by complex coacervation of sodium alginate/quaternary ammonium salt of chitosan[J]. Royal Society of Chemistry Advances,2016,6(16):13032-13039.
[33]	Messaoud G B,Sánchez-González L,Probst L,et al. Influence of internal composition on physicochemical properties of alginate aqueous-core capsules[J]. Journal of Colloid and Interface Science,2016,469:120-128.
[34]	Tønnesen H H,Karlsen J. Alginate in drug delivery systems[J].Drug Development and Industrial Pharmacy,2002,28(6):621-630.
[35]	Braccini I,Pérez S. Molecular basis of Ca²⁺-induced gelation in alginates and pectins:the egg-box model revisited[J].Biomacromolecules,2001,2(4):1089-1096.
[36]	李云晖,浦跃朴,尹立红,等.氧化铁纳米颗粒固定化溶藻菌的除藻作用[J].东南大学学报(自然科学版),2006,36(6):986-990.