中国科学引文数据库(CSCD)来源期刊
中国科技核心期刊
环境科学领域高质量科技期刊分级目录T2级期刊
RCCSE中国核心学术期刊
美国化学文摘社(CAS)数据库 收录期刊
日本JST China 收录期刊
世界期刊影响力指数(WJCI)报告 收录期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

细菌Chryseobaterium sp.溶藻方式及其溶藻胶囊制备条件研究

王金霞 陈玉成 詹玲玲 罗乐

王金霞, 陈玉成, 詹玲玲, 罗乐. 细菌Chryseobaterium sp.溶藻方式及其溶藻胶囊制备条件研究[J]. 环境工程, 2019, 37(9): 91-96+193. doi: 10.13205/j.hjgc.201909017
引用本文: 王金霞, 陈玉成, 詹玲玲, 罗乐. 细菌Chryseobaterium sp.溶藻方式及其溶藻胶囊制备条件研究[J]. 环境工程, 2019, 37(9): 91-96+193. doi: 10.13205/j.hjgc.201909017

细菌Chryseobaterium sp.溶藻方式及其溶藻胶囊制备条件研究

doi: 10.13205/j.hjgc.201909017
基金项目: 

国家科技支撑计划(2015BAL04B01)

重庆市教育委员会科学技术研究计划青年项目(KJQN201803406)

详细信息
    作者简介:

    王金霞(1980-),女,博士,副教授,主要研究方向为生态修复理论与技术。42219423@qq.com

  • 中图分类号: X172;X52

  • 摘要: 为探究细菌Chryseobaterium sp.的溶藻能力及方式,通过扫描电镜观察细菌Chryseobaterium sp.对藻Anabaena flosaquae的作用效果,进一步通过分离菌体和菌上清液的方法探讨其溶藻的作用机制。结果表明:细菌Chryseobaterium sp.具有很强的溶藻能力,其主要通过分泌溶藻活性物质进行间接溶藻。胶囊制备的优化条件为:SA、CaCl2、EC和柠檬酸质量分数分别为3%、3%、3%和2%,CaCO3与SA的质量分数比为4/5,交联时间为30 min,溶藻物质质量分数为16%,该条件下所制胶囊缓慢释放溶藻物质,具有长效的溶藻能力。揭示了细菌Chryseobaterium sp.主要通过分泌溶藻活性物质进行间接溶藻,所制胶囊具有较强的溶藻能力,为细菌Chryseobaterium sp.溶藻机理和生物控藻技术的研究提供了理论依据。
  • [1] Xuan H L,Dai X Z,Li J,et al. A Bacillus sp. strain with antagonistic activity against Fusarium graminearum kills Microcystis aeruginosa selectively[J]. Science of the Total Environment,2017,583:214-221.
    [2] Li S Y,Liu W Z,Gu S,et al. Spatio-temporal dynamics of nutrients in the upper Han River basin,China[J]. Journal of Hazardous Materials,2009,162(2):1340-1346.
    [3] Sun R,Sun P F,Zhang J H,et al. Microorganisms-based methods for harmful algal blooms control:a review[J]. Bioresource Technology,2018,248:12-20.
    [4] Liao C L,Liu X B,Liu R F,et al. Two novel algicidal isolates kill Chlorella pyrenoidosa by inhibiting their host antioxidase activities[J]. Applied Biochemistry and Biotechnology,2015,177(2):567-576.
    [5] 裴海燕,胡文容,曲音波,等.一株溶藻细菌的分离鉴定及其溶藻特性[J].环境科学学报,2005,25(6):796-802.
    [6] 吴刚,席宇,赵以军.溶藻细菌研究的最新进展[J].环境科学研究,2002,15(5):43-46.
    [7] 焦彦凯,严小军,李小兵.溶藻细菌及溶藻化合物研究进展[J].工业微生物,2018,48(4):60-66.
    [8] 刘萍,张吉强.固定化溶藻菌除藻效果研究[J].环境工程,2016,33(12):28-31.
    [9] 3种载体固定化菌藻共生系统脱氮除磷效果的对比[J].环境工程学报,2013,7(9):3256-3262.
    [10] 刘燕.海洋溶藻弧菌的培养优化及溶藻粉剂胶囊球工艺研究[D].深圳:深圳大学,2017.
    [11] Andersen T,Strand B L,Formo K,et al. Alginates as biomaterials in tissue engineering[J]. Carbohydrate Chemistry:Chemical and Biological Approaches,2011,37:227-258.
    [12] Orive G, Carcaboso A. M, Hernandez R. M, et al.Biocompatibility evaluation of different alginates and alginate-based microcapsules[J]. Biomacromolecules,2005,6:927-931.
    [13] Ching S H,Bansal N,Bhandari B. Alginate gel particles-A review of production techniques and physical properties[J]. Critical Reviews in Food Science and Nutrition,2017,57(6):1133-1152.
    [14] 齐素芳,余煜棉,赖子尼,等.复合载体固定化硝化细菌去除水体中氨氮的研究[J].广东工业大学学报,2007,24(2):15-19.
    [15] Sun H Y,Zhang Y,Chen H R,et al. Isolation and characterization of the marine algicidal bacterium Pseudoalteromonas S1against the harmful alga Akashiwo sanguinea[J]. Marine Biology,2016,163(3):66-74.
    [16] Qianlong F,Hengfeng Z,Juan H,et al. Algae-lysing effect of algicidal bacteria on Microcystis in bloom water[J]. Meteorological&Environmental Research,2016,7(6):29-32.
    [17] Bowman J P. Bioactive compound synthetic capacity and ecological significance of marine bacterial genus Pseudoalteromonas[J].Marine Drugs,2007,5(4):220-241.
    [18] 史顺玉.溶藻细菌对藻类的生理生态效应及作用机理研究[D].武汉:中国科学院水生生物研究生所,2006.
    [19] Henrichsen J,Blom J. Examination of fimbriation of some gramnegative rods with and without twitching and gliding motility[J].Acta Pathologica Microbiologica Scandinavica Section B Microbiology,1975,83(3):161-170.
    [20] Panova T T,Ivanova J. Influence of physiological factors on the lysis effect of cytophaga on the red microalga Rhodella reticulata[J]. Applied and Environmental Microbiology,2000,88(2):358-363.
    [21] Costerton J W,Irvin R T,Cheng K J. The bacterial glycocalyx in nature and disease[J]. Annual Reviews in Microbiology,1981,35(1):299-324.
    [22] Kim Y S,Son H J,Jeong S Y. Isolation of an algicide from a marine bacterium and its effects against the toxic dinoflagellate Alexandrium catenella and other harmful algal bloom species[J].Journal of Microbiology,2015,53(8):511-517.
    [23] Sameeh M Y,Mohamed A A,Elazzazy A M. Polyphenolic contents and antimicrobial activity of different extracts of Padina boryana Thivy and Enteromorpha sp. marine algae[J]. Journal of Applied Pharmaceutical Science,2016,6(9):87-92.
    [24] Yoshikawa K, Adachi K, Nishij M, et al.β-Cyanoalanine production by marine bacteria on cyanide-free medium and its specific inhibitory activity toward cyanobacter[J]. Applied and Environmental Microbiology,2000,66(2):718-722.
    [25] Imamura N,Motoike I,Shimada N,et al. An efficient screening approach for anti-Microcystis compounds based on knowledge of aquatic microbial ecosystem[J]. the Journal of Antibiotics,2001,54(7):582-587.
    [26] Mitsutani A,Yamasaki I,Kitaguchi H,et al. Analysis of algicidal proteins of a diatom-lytic marine bacterium Pseudoalteromonas sp.strain A25 by two-dimensional electrophoresis[J]. Phycological,2001,40(3):286-291.
    [27] Kato J,Amie J,Murata Y,et al. Development of a genetic transformation system for an alga-lysing bacterium[J]. Applied and environmental microbiology,1998,64(6):2061-2064.
    [28] Paul S,Jinnque R,Haim B G. Bacterial suppression of Chlorella by hydroxylamine production[J]. Water Research,1979,13(1):267-273.
    [29] Sakata T,Yoshikawa T,Nishitarumizu S. Algicidal activity and identification of an algicidal substance produced by marine Pseudomonas sp. C55a-2[J]. Fisheries Science,2011,77(3):397-402.
    [30] Kodani S,Imoto A,Mitsutani A. Isolation and identification of the antialgal compound,harmane(1-methyl-β-carboline),produced by the algicidal bacterium,Pseudomonas sp. K44-1[J]. Journal of Applied Phycology,2002,14(2):109-114.
    [31] 熊振湖,孙翠珍,刘青春.不同载体固定化藻菌共生系统的脱氮除磷效果[J].环境科学与技术,2005,28(1):82-84.
    [32] Chen M,Hu Y,Zhou J,et al. Facile fabrication of tea tree oilloaded antibacterial microcapsules by complex coacervation of sodium alginate/quaternary ammonium salt of chitosan[J]. Royal Society of Chemistry Advances,2016,6(16):13032-13039.
    [33] Messaoud G B,Sánchez-González L,Probst L,et al. Influence of internal composition on physicochemical properties of alginate aqueous-core capsules[J]. Journal of Colloid and Interface Science,2016,469:120-128.
    [34] Tønnesen H H,Karlsen J. Alginate in drug delivery systems[J].Drug Development and Industrial Pharmacy,2002,28(6):621-630.
    [35] Braccini I,Pérez S. Molecular basis of Ca2+-induced gelation in alginates and pectins:the egg-box model revisited[J].Biomacromolecules,2001,2(4):1089-1096.
    [36] 李云晖,浦跃朴,尹立红,等.氧化铁纳米颗粒固定化溶藻菌的除藻作用[J].东南大学学报(自然科学版),2006,36(6):986-990.
  • 加载中
计量
  • 文章访问数:  30
  • HTML全文浏览量:  9
  • PDF下载量:  0
  • 被引次数: 0
出版历程
  • 收稿日期:  2019-04-23
  • 网络出版日期:  2023-11-24
  • 刊出日期:  2019-09-30

目录

    /

    返回文章
    返回