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氢气和氮气对厌氧甲烷降解过程及微生物多样性的影响

尹莹,  迟子芳,  黄华铮,  王文静

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文章导航 > 环境工程  > 2020 >  38(5): 191-195
尹莹, 迟子芳, 黄华铮, 王文静. 氢气和氮气对厌氧甲烷降解过程及微生物多样性的影响[J]. 环境工程, 2020, 38(5): 191-195. doi: 10.13205/j.hjgc.202005033
引用本文: 尹莹, 迟子芳, 黄华铮, 王文静. 氢气和氮气对厌氧甲烷降解过程及微生物多样性的影响[J]. 环境工程, 2020, 38(5): 191-195. doi: 10.13205/j.hjgc.202005033
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YIN Ying, CHI Zi-fang, HUANG Hua-zheng, WANG Wen-jing. EFFECTS OF HYDROGEN AND NITROGEN ON ANAEROBIC METHANE DEGRADATION COURSE AND MICROBIAL DIVERSITY[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(5): 191-195. doi: 10.13205/j.hjgc.202005033
Citation: YIN Ying, CHI Zi-fang, HUANG Hua-zheng, WANG Wen-jing. EFFECTS OF HYDROGEN AND NITROGEN ON ANAEROBIC METHANE DEGRADATION COURSE AND MICROBIAL DIVERSITY[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(5): 191-195. doi: 10.13205/j.hjgc.202005033
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氢气和氮气对厌氧甲烷降解过程及微生物多样性的影响

doi: 10.13205/j.hjgc.202005033

  • 吉林大学 地下水资源与环境教育部重点实验室, 长春 130021

基金项目: 

国家自然科学基金资助项目(41977158,41772244)。

详细信息
    作者简介:

    尹莹(1994-),女,硕士研究生,主要研究方向为固体废弃物处理处置。281556768@qq.com

    通讯作者:

    迟子芳,chizifang@jlu.edu.cn

EFFECTS OF HYDROGEN AND NITROGEN ON ANAEROBIC METHANE DEGRADATION COURSE AND MICROBIAL DIVERSITY

  • Key Lab of Groundwater Resources and Environment, Ministry of Education, Jilin University, Changchun 130021, China

    摘要
  • 摘要: 针对厌氧甲烷氧化过程,考察了填埋场稳定化过程中气态物质(H2、N2)的影响,阐明了微生物群落结构的响应。结果表明:在N2氛围下,存在甲烷氧化、固氮、硝化、反硝化的循环过程;通入H2对于厌氧甲烷降解过程有一定影响,CH4含量呈先下降后升高再降低的趋势,CO2含量则呈先升高后降低趋势,表明H2可将CO2还原为CH4。经过N2和H2长期驯化后,土壤微生物群落结构发生了显著变化,放线菌门中具有硝化、反硝化及固氮作用的菌群增加,出现了具有甲烷氧化功能的Methylococcale菌群。好氧菌(如甲烷氧化菌、硝化菌)对O2的消耗有利于反硝化和厌氧甲烷氧化的进行,为反硝化型厌氧甲烷氧化的发生提供了有利条件。研究揭示了填埋场稳定化过程中气态物质影响厌氧甲烷氧化的过程。
    Abstract: In the process of landfill stabilization, to study the influencing factors of anaerobic methane oxidation process, the influence of gaseous substances (hydrogen, nitrogen) on the anaerobic methane oxidation process was investigated. On this basis, the effect of simulated gas on the anaerobic methane degradation process and microbial population structure were clarified. The results showed that there were cycle processes of methane oxidation, nitrogen fixation, nitrification and denitrification under atmospheric nitrogen, while the introduction of hydrogen had a certain impact on the degradation process of anaerobic methane. The methane concentration decreased firstly, then increased and then decreased, and the carbon dioxide concentration first increased then decreased, which was due to the reduction of carbon dioxide by hydrogen. After the long-term domestication of nitrogen and hydrogen, the structures of the soil microbial community changed significantly, and the amount of bacteria with nitrification, denitrification and nitrogen fixation function increased, and the Methylococcale appeared. The consumption of oxygen by aerobic bacteria (such as methane oxidizing bacteria and nitrifying bacteria) was conducive to the process of denitrification and anaerobic methane oxidation, which provided favorable condition for the occurrence of anaerobic methane oxidation. The results revealed the influence of gaseous substances on the anaerobic methane oxidation process during landfill stabilization.
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  • 收稿日期:  2019-11-01

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