重金属复合污染场地对花背蟾蜍雄性性腺的毒性作用

黄涛; 刘菊梅; 彭琴; 喻秋; 昝晓慧; 王志勇; 曹优明; 司万童; 陈辉霞

doi:10.13205/j.hjgc.202006006

重金属复合污染场地对花背蟾蜍雄性性腺的毒性作用

doi: 10.13205/j.hjgc.202006006

黄涛^2,3,4,
刘菊梅¹,
彭琴²,
喻秋⁴,
昝晓慧⁴,
王志勇⁴,
曹优明¹,
司万童^1,,
陈辉霞^2,3,

1. 重庆文理学院化学与环境工程学院环境材料与修复技术重庆市重点实验室, 重庆 402160;
2. 中国科学院绿色过程与工程重点实验室, 北京 100190;
3. 中国科学院过程工程研究所湿法冶金清洁生产技术国家工程实验室, 北京 100190;
4. 内蒙古科技大学生命科学与技术学院, 内蒙古包头 014010

基金项目:

国家重点研发计划(2018YFC1802200)

重庆市自然科学基金(CSCC2019hcyj-msxmX0808)

重庆文理学院人才引进专项基金(R2018SCH03)

国家自然科学基金(31460142)。

详细信息

作者简介:
黄涛(1992-),男,硕士,助理工程师,主要从事水污染生物监测研究。niklou50509@163.com

通讯作者:
司万童(1986-),男,博士,副教授,主要从事水污染生物监测研究。siwt03@163.com

陈辉霞(1984-),女,博士,助理研究员,主要从事土壤和地下水重金属污染修复研究。hxchen@ipe.ac.cn

计量
- 文章访问数: 194
- HTML全文浏览量: 46
- PDF下载量: 1
- 被引次数: 0
出版历程
- 收稿日期: 2020-04-02

TOXIC EFFECTS OF HEAVY METALS CONTAMINATED SITE ON MALE GONAD OF BUFO RADDEI

1. Chongqing Key Laboratory of Environmental Materials & Remediation Technologies, College of Chemistry and Environmental Engineering, Chongqing University of Arts and Sciences, Chongqing 402160, China;
2. CAS Key Laboratory of Green Process and Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
3. National Engineering Laboratory for Hydrometallurgical Cleaner Production Technology, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, China;
4. School of Life Science and Technology, Inner Mongolia University of Science & Technology, Baotou 014010, China

摘要

摘要: 以花背蟾蜍为研究对象,以包头市某尾矿库南侧受污染的水域湿地和相对无污染的小白河黄河湿地保护区为研究样地。对比分析雄性花背蟾蜍形态学指标、精巢脏器系数、精子动态参数、精子畸形率、性激素、红细胞微核率和DNA损伤等相关指标,研究重金属复合污染场地对花背蟾蜍雄性性腺的毒性效应。结果显示:与小白河湿地相比,尾矿库湿地花背蟾蜍条件因子和精巢脏器系数显著高于小白河湿地(P<0.01)。尾矿库花背蟾蜍精子的鞭打频率显著升高,而精子活力显著降低(P<0.05),精子畸形率、红细胞微核率和DNA损伤均显著高于小白河湿地(P<0.05)。结果表明:尾矿库复合污染通过降低精子质量,增加红细胞微核率和DNA损伤对花背蟾蜍的雄性性腺造成毒理学影响,花背蟾蜍通过升高条件因子和精巢脏器系数来缓解复合污染的毒性胁迫。该成果对重金属复合污染场地两栖类动物种群保护以及生态平衡维护有着积极作用。
- 复合污染 /
- 条件因子 /
- 精子质量 /
- 微核率 /
- DNA损伤
Abstract: In this study, Bufo Raddei was taken as the research object, and the polluted water wetland and the relatively pollution-free Yellow River Wetland Reserve on the south side of the tailing wetland in Baotou, were taken as the research sites. Through analysis of morphological indexes, testis organ coefficient, sperm dynamic parameters, sperm deformity rate, sex hormone, micro-nucleus rate, and DNA damage of different male B. raddei, the toxic effect of compound pollution of tailing wetland on male gonads of B. raddei was studied. The results showed that the condition factor (CF) and the testis organ coefficient of B. raddei in tailing wetland was significantly higher than that in Xiaobaihe wetland (P < 0.01). In addition, the whipping frequency of sperms in the tailing wetland was significantly improved, while the sperm motility was significantly decreased (P<0.05). The sperm deformity rate, erythrocyte micro-nucleus, and DNA damage were obviously higher than that in the Xiaobaihe wetland (P<0.05). The results showed that the compound pollution of tailing wetland resulted in decrease of the sperm quality and increase of the micro-nucleus rate and DNA damage, which influenced the toxicological effect of male gonads. The toxic stress of compound pollution was eased by increasing the condition factor and the coefficient of spermatozoa. The above results had positive effect on the population protection, and ecological balance maintenance of amphibians in the compound pollution site of heavy metals.
- compound pollution /
- condition factor /
- sperm quality /
- micronucleus rate /
- DNA damage

HTML全文

参考文献(36)

王艳伟,李书鹏,康绍果,等. 中国工业污染场地修复发展状况分析[J]. 环境工程,2017,35(10):175-178.

黄涛. 包头市典型湿地复合污染的生物监测与评价[D]. 包头:内蒙古科技大学,2019.

韩瑞杰,求瑞娟,任逸晨,等. 尾矿库湿地重金属污染的生物富集特征及风险评价[J]. 生态与农村环境学报, 2019, 35(1): 22-27.

司万童,代静,赵雪波,等. 稀土尾矿库湿地土壤重金属污染生态风险评价[J]. 人民黄河,2017,39(8):71-75

,89.

韩瑞杰,任逸晨,黄涛,等. 包头市三类湿地中重金属污染程度及生物富集研究[J]. 环境工程,2019,37(1):29-34.

苗菲菲,司万童,刘菊梅,等. 尾矿库渗漏水导致泥鳅氧化损伤与DNA损伤的研究[J]. 广东农业科学,2012,39(16):162-164.

李成,江建平.无尾两栖类在不同生活史阶段的栖息环境[J]. 四川动物,2016,35(6):950-955.

何一帆. 云南8种无尾两栖类呼吸和水分调节器官的环境适应性研究[D]. 昆明:云南师范大学,2018.

米志平,廖文波.林蛙属3物种皮肤的组织结构比较[J]. 动物学杂志,2016,51(5):844-852.

司万童,蒋海明,栗利曼,等. 花背蟾蜍性腺抗氧化酶对稀土尾矿库污染胁迫的响应[J]. 生态毒理学报,2017,12(2):209-215.

黄涛,求瑞娟,兰宗宝,等.稀土尾矿库渗漏水污染对花背蟾蜍胚后发育的毒性作用[J]. 南方农业学报,2019,50(2):412-417.

冯磊. 重金属富集及对花背蟾蜍跳跃能力的影响[D]. 兰州:兰州大学,2012.

CALEVRO F,CAMPANI S. Tests of toxicity and teratogenicity in biphasic verteberatios treated with heavy metal(Cr³⁺,AI³⁺,Cd²⁺)[J]. Chemosphere,1998,37:301-305.

黄德军,张迎梅,赵东芹,等.重金属镉对花背蟾蜍蝌蚪生长发育的影响[J]. 兰州大学学报(自然科学版),2004,40(2):81-83.

GUO R,ZHANG W Y,YANG Y,et al. Variation of fitness and reproductive strategy in male Bufo raddei under environmental heavy metal pollution[J]. Ecotoxicology and Environmental Safety,2018,164:253-260.

ZHANG W Y,GUO R,AI S W, et al. Long-term heavy metal pollution varied female reproduction investment in free-living anura, Bufo raddei[J].Ecotoxicology and environmental safety,2018,159:136-142.

李艳斌. Cr⁶⁺慢性暴露对中华大蟾蜍胚胎和蝌蚪的毒理效应研究[D].西安:长安大学,2019.

EGEA-SERRANO A,RELYEA R A,TEJEDO M,et al. Understanding of the impact of chemicals on amphibians: a meta-analytic review[J]. Ecology and Evolution,2012,2(7):1382-1397.

李万江. 重金属铅、汞对花背蟾蜍蝌蚪毒性作用的初步研究[D].西安:西北师范大学,2015.

耿宝荣,姚丹,纪冰,等. 重金属铬离子对两种无尾两栖类蝌蚪的急性毒性影响[C]//中国动物学会两栖爬行动物学分会2005年学术研讨会,2005.

贾秀英,董爱华,马小梅. 镉致蟾蜍肝、肾脂质过氧化损伤[J]. 应用与环境生物学报,2004,10(1):92-94.

DAVIDSON C,SHAFFER H B,JENNINGS M R. Declines of the California red legged frog:climate,UV2B,habitat,and Pesticides hypotheses[J]. Ecol Appl, 2001,11:464-479.

郭伟,付瑞英,赵仁鑫,等. 内蒙古包头白云鄂博矿区及尾矿区周围土壤稀土污染现状和分布特征[J]. 环境科学,2013,34(5):1895-1900.

国家环境保护总局. 地表水环境质量标准:GB 3838—2002[S]. 北京:中国标准出版社,2002.

魏复盛,陈静生,吴燕玉,等. 中国土壤环境背景值研究[J]. 环境科学,1991,12(4):12-19.

顾永瑞,张彤,白红玉. 内蒙土壤环境背景值属性分类[J]. 内蒙古环境保护,1995,7(1):6-9.

徐光宪,师昌绪. 关于保护白云鄂博矿钍和稀土资源避免黄河和包头受放射性污染的紧急呼吁[J]. 中国科学院院刊,2005,20(6):448-450.

刘娟,张雪峰,司万童,等.尾矿库周边地下水对SD大鼠的毒性效应研究[J]. 环境科学与技术,2014,34(12):129-133.

郭蕊. 环境重金属污染对花背蟾蜍繁殖对策的影响[D]. 兰州:兰州大学,2019.

孙乃亮. 镉和汞对中华大蟾蜍蝌蚪形态、甲状腺、骨骼、肝脏的慢性毒理效应[D]. 西安:陕西师范大学,2018.

PALAS S,HYUNGJOON I,JOORIM N,et al. Ecological risk assessment of a contaminated stream using multi-level integrated biomarker response in Carassius auratus[J]. Environmental Pollution,2018,233:429-438.

刘占才,牛景彦. 汞暴露对草鱼脾和肾脏器系数的影响[J]. 湖北农业科学,2011,50(4):810-811

,814.

项华,王慧敏,金慧,等. 邻苯二甲酸二甲酯对雄性小鼠生殖系统的影响[J]. 中国卫生检验杂志,2019,29(21):2584-2587

,2596.

刘建博,潘登,江安娜,等. 镉暴露对文蛤雄性生殖细胞的影响[J]. 环境科学学报,2013,33(7):2036-2043.

ZORNIK E,KELLEY D B. A neuroendocrine basis for the hierarchical control of frog courtship vocalizations[J]. Front Neuroendocrinol,2011,32(3): 353-366.

叶圣涛. 几种农药对黑斑侧褶蛙和泽陆蛙的遗传毒理效应研究[D]. 金华:浙江师范大学,2013.

施引文献

资源附件(0)

访问统计