石油降解低温细菌的筛选及降解特性的表征

贾凌慧; 郑世浩; 孙丽慧; 包永明

doi:10.13205/j.hjgc.202006041

石油降解低温细菌的筛选及降解特性的表征

doi: 10.13205/j.hjgc.202006041

1. 大连理工大学食品与环境学院, 辽宁盘锦 124221;
2. 大连理工大学生命科学与技术学院, 辽宁大连 116024

基金项目:

国家自然科学基金"铁-碳纳米复合物活化过硫酸盐氧化土壤多环芳烃的化学机制"(21876022)。

详细信息

作者简介:
贾凌慧,女,硕士研究生,主要研究方向为环境微生物。linghuijia@mail.dlut.edu.cn

通讯作者:
包永明,男,教授,主要研究方向为生物催化转化。biosci@dlut.edu.cn

计量
- 文章访问数: 142
- HTML全文浏览量: 20
- PDF下载量: 3
- 被引次数: 0
出版历程
- 收稿日期: 2019-06-11

SCREENING OF COLD-ADAPTED PETROLEUM DEGRADING BACTERIA AND CHARACTERIZING MICROFLORA FOR PETROLEUM DEGRADATION

1. School of Food and Environment Science and Technology, Dalian University of Technology, Panjin 124221, China;
2. School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024, China

摘要

摘要: 从石油污染土壤中,通过低温富集,筛选并鉴定得到7株低温石油降解细菌。基于菌株降解石油组分特性,构建6组低温石油降解菌群,利用5 L发酵罐,并通过尾气分析仪在线监测菌群石油降解过程中的CO₂产生和O₂消耗变化,评价菌群的石油降解能力。由Arthrobacter sp. JLH 001,Acinetobacter baumannii JLH 002,Pseudomonas fragi JLH 003和Arthrobacter sp. JLH 006组成的菌群降解石油效果最佳,48 h后CO₂的产生值和O₂的消耗值达到最高,在15 ℃时、72 h后能完全降解1%的石油,并且在25 ℃时降解速度显著增强。结果表明:石油污染土壤的原位生物修复可通过低温石油降解菌群的添加实现高效及快速修复。
- 石油污染土壤 /
- 低温细菌 /
- 石油降解微生物 /
- 生物降解 /
- 生物修复
Abstract: The performance of in-situ bio-remediation of petroleum-contaminated soils has been always unsatisfactory, because of complex composition of crude oil and its strong biological toxicity, as well as low microbial activity at low soil temperature. In this paper, 7 cold-adapted oil-degrading bacteria strains were screened and identified from oil polluted soils. Based on the degrading characteristics of the 7 bacteria strains for petroleum composition, 6 cold-adapted oil-degrading microfloras were prepared. The CO₂ production and O₂ consumption of 6 microfloras for degrading crude oil in 5 L fermenters were monitored online with a gas analyzer. The most efficient microflora, consisted of Arthrobacter sp. JLH 001, Acinetobacter baumannii JLH 002, Pseudomonas fragi JLH 003 and Arthrobacter sp. JLH 006, showed maximal O₂ consumption and CO₂ production capacity after about 48 h, and completely degraded crude oil (with content of 1%) after about 72 h at 15 ℃, and the degradation was significantly accelerated at 25 ℃. These results suggested that efficient in-situ bio-remediation of petroleum-contaminated soil could be realized by adding cold-adapted oil-degrading microfloras.
- petroleum contaminated soil /
- cold-adapted bacteria /
- petroleum degrading bacteria /
- bio-degradation /
- bioremediation

HTML全文

参考文献(24)

VARJANI S J. Microbial degradation of petroleum hydrocarbons [J]. Bioresource Technology, 2017, 223: 277-286.

李宝明. 石油污染土壤微生物修复的研究[D]. 北京: 中国农业科学院, 2007.

张学佳,纪巍,康志军,等. 石油类污染物对土壤生态环境的危害[J]. 化工科技, 2008, 16(6): 60-65.

CHANDRA S, SHARMA R, SINGH K, et al. Application of bioremediation technology in the environment contaminated with petroleum hydrocarbon [J]. Annals of Microbiology, 2013, 63(2): 417-431.

MECKENSTOCK R U, BOLL M, MOUTTAKI H, et al. Anaerobic degradation of benzene and polycyclic aromatic hydrocarbons [J]. Journal of Molecular Microbiology and Biotechnology, 2016, 26(1/2/3): 92-118.

ZHANG Z Z, HOU Z W, YANG C Y, et al. Degradation of n-alkanes and polycyclic aromatic hydrocarbons in petroleum by a newly isolated Pseudomonas aeruginosa DQ8[J]. Bioresource Technology, 2011, 102(5): 4111-4116.

COSTA A S, ROMAO L P, ARAUJO B R, et al. Environmental strategies to remove volatile aromatic fractions (BTEX) from petroleum industry wastewater using biomass [J]. Bioresource Technology, 2012, 105: 31-39.

SOUZA E C, VESSONI-PENNA T C, Oliveira R P S. Biosurfactant-enhanced hydrocarbon bioremediation: An overview [J]. International Biodeterioration & Biodegradation, 2014, 89: 88-94.

杜亚鲁, 胡韬, 彭琳. 土壤石油污染的生物修复技术研究进展[J]. 环境科学与技术, 2017, 40(增刊1): 133-138.

ABBASIAN F, LOCKINGTON R, MALLAVARAPU M, et al. A comprehensive review of aliphatic hydrocarbon biodegradation by bacteria [J]. Applied Biochemistry Biotechnology, 2015, 176(3): 670-699.

WILKES H, BUCKEL W, GOLDING B T, et al. Metabolism of hydrocarbons in n-alkane-utilizing anaerobic bacteria [J]. Journal of Molcular Microbiology and Biotechnology, 2016, 26(1/2/3): 138-151.

GHAZALI F M, RAHMAN R N Z A, SALLEH A B, et al. Biodegradation of hydrocarbons in soil by microbial consortium [J]. International Biodeterioration & Biodegradation, 2004, 54(1): 61-67.

JANBANDHU A, FULEKAR M H. Biodegradation of phenanthrene using adapted microbial consortium isolated from petrochemical contaminated environment [J]. Journal of Hazardous Materials, 2011, 187(1/2/3): 333-340.

MITTAL A, SINGH P. Isolation of hydrocarbon degrading bacteria from soils contaminated with crude oil spills [J]. Indian Journal of Experimental Biology, 2009, 47(9): 760-765.

HARAYAMA S, KASAI Y, HARA A. Microbial communities in oil-contaminated seawater [J]. Current Opinion in Biotechnology, 2004, 15(3): 205-214.

赵晓秀, 赵慧敏, 全燮, 等. 石油污染土壤中菲、蒽和正十六烷的微生物降解[J]. 生态学杂志, 2009,28(3):456-460.

钱奕忠. 微生物降解石油废水的研究[D]. 北京: 北京化工大学, 2001.

朱杰, 阮志勇,董卫卫,等. 一株高效烷烃降解菌Acinetobacter sp.LAM1007的分离鉴定及降解特性[J]. 微生物学通报, 2017,44(7):1535-1546.

温文静, 谭丽婵, 雒晓芳, 等. 嗜碱性假单胞菌对菲的降解研究[J]. 西北民族大学学报(自然科学版), 2016,37(3):58-61.

徐成斌, 王闻烨, 李鲜珠, 等. 一株菲降解菌的鉴定及降解特性[J]. 环境科学学报, 2015, 35(3):684-691.

杨乐. 产表面活性剂解烃菌的筛选及其降解条件研究[J]. 环境工程, 2015(6):153-157.

LIU Y, ZENG G M, ZHONG H, et al. Effect of rhamnolipid solubilization on hexadecane bioavailability: enhancement or reduction? [J]. Journal of Hazardous Materials, 2017, 322(Pt B): 394-401.

INōS, MNIF, DHOUHA G. Glycolipids biosurfactants; potential related biomedical and biotechnological applications[J]. Carbohydrate Research, 2015.

李敬龙, 刘晔, 潘爱珍. 生物表面活性剂及其应用[J]. 齐鲁工业大学学报, 2004, 18(2):41-46.

施引文献

资源附件(0)

访问统计