不同电子受体驯化聚糖菌反硝化过程及N<sub>2</sub>O释放特性

鞠洪海

doi:10.13205/j.hjgc.202009019

不同电子受体驯化聚糖菌反硝化过程及N₂O释放特性

doi: 10.13205/j.hjgc.202009019

鞠洪海

烟台职业学院建筑工程系, 山东烟台 264670

基金项目:

国家自然科学基金项目（51668031）；烟职博士基金2018002号。

详细信息

作者简介:
鞠洪海(1971-),男,硕士,讲师。260943813@qq.com

计量
- 文章访问数: 189
- HTML全文浏览量: 11
- PDF下载量: 5
- 被引次数: 0
出版历程
- 收稿日期: 2020-04-25

CHARACTERICS OF DENITRIFICATION AND N₂O EMISSION OF ACCLIMATED GLYCOGEN ACCUMULATING ORGANISMS USING DIFERENT ELECTRON ACCEPTOR

JU Hong-hai

Department of Architecture Engineering, Yantai Vocational College, Yantai 264670, China

摘要

摘要: 利用序批式（sequencing batch reactor，SBR）生物反应器，采用厌氧-好氧运行方式，以乙酸钠为碳源，在控制进水P/COD<2/100条件下，成功实现了聚糖菌（glycogen accumulating organisms，GAOs）富集。缺氧初始阶段ρ（NO_x^--N）为30.0 mg/L，经厌氧-缺氧驯化后，反硝化聚糖菌（denitrifuing GAOs，DGAOs）可利用聚-β-羟基脂肪酸酯（poly-β-hydroxyalkanoate，PHA）为内碳源进行反硝化，且分解利用的PHA中80%以上为聚-β-羟基丁酸酯（poly-β-hydroxybutyrate，PHB）。高浓度NO₂^-抑制DGAOs活性，厌氧PHA合成降低，且缺氧段PHA分解产生的能量较多地用于储存糖原（glycogen，Gly）。NO₃^-和NO₂^-还原过程中，PHA降解速率分别为19.28，10.02 mg/（g·h），内源反硝化速率分别为3.32，2.29 mg/（g·h），TN去除率达95%以上。随NO₂^-/NO_x^-增加，N₂O平均产率由29.1%增至59.0%。高浓度NO₂^-对氧化亚氮还原酶（Nos）活性抑制作用以及Nos和亚硝态氮还原酶（Nir）之间的电子竞争过程，是导致NO₂^-内源反硝化过程中N₂O大量释放的主要原因。
- 聚糖菌 /
- 电子受体 /
- 内源反硝化 /
- 氧化亚氮N₂O /
- 电子分配
Abstract: Using an anaerobic-aerobic sequencing batch reactor (AO-SBR), the glycogen accumulating organisms (GAOs) had been enriched with sodium acetate as the carbon source and the P/C ratio lower than 2/100. Cultured in anaerobic-anoxic operation mode, the acclimated GAOs could perform endogenous denitrification reaction with NO_x^--N as electron acceptor and poly-β-hydroxyalkanoate(PHA) as electron donor, where PHB (poly-β-hydro-xybutyrate) accounted for more than 80% of the PHA decomposition and utilization. The higher NO₂^- concentration inhibited the activity of DGAOs, which led to the decrease of PHA synthesis. More energy from PHA decomposition would be used for glycogen storage. During the reduction process of NO₃^- and NO₂^-, the PHA degradation rate was 19.28 mg/(g·h) and 10.02 mg/(g·h) respectively, which resulted in the related endogenous denitrification rates were 3.32 mg/(g·h) and 2.29 mg/(g·h). The TN removal efficiency reached more than 95% in the end. With the increase of NO₂^-/NO_x^-, the N₂O yield during the endogenous denitrification process increased from 29.1% to 59.0%. The higher NO₂^- inhibition on the activity of Nos, as well as the electron competition between Nir and Nos, resulted in the higher N₂O production during PHA-driven denitrification by DGAOs.
- glycogen accumulating organisms (GAOs) /
- electron acceptor /
- endogenous denitrification /
- N₂O /
- electron distribution

HTML全文

参考文献(20)

ZENG R J, YUAN Z G, KELLER J. Enrichment of denitrifying glycogen-accumulating organisms in anaerobic/anoxic activated sludge system[J]. Biotechnology and Bioengineering, 2003, 81(4):397-404.

MIAO L, WANG S Y, LI B K, et al. Advanced nitrogen removal via nitrite using stored polymers in a modified sequencing batch reactor treating landfill leachate[J]. Bioresource Technology, 2015, 192:354-360.

刘小芳,郭海燕,张胜男,等. 聚糖菌反硝化影响因素及内碳源转化特性[J]. 化工学报, 2019,70(3):1127-1134.

ZHOU Y, PIJUAN M, ZENG R J, et al. Free nitrous acid inhibition on nitrous oxide reduction by a denitrifying-enhanced biological phosphorus removal sludge[J]. Environmental Science & Technology, 2008,42:8260-8265.

YE L, NI B J, LAW Y Y, et al. A novel methodology to quantify nitrous oxide emissions from full-scale wastewater treatment systems with surface aerators[J]. Water Research, 2014, 48(1):257-268.

PAN Y, NI B J, BONDP L, et al. Electron competition among nitrogen oxides reduction during methanol-utilizing denitrifification in wastewater treatment[J]. Water Research, 2013,47:3273-3281.

MCILROY S J, ALBERTSEN M, ANDRESEN E K, et al. "Candidatus Competibacter"-lineage genomes retrieved from metagenomes reveal functional metabolic diversity[J]. ISME J. 2014,8:613-624.

YANG Q, LIU X H, PENG C Y, et al. N₂O production during nitrogen removal via nitrite from domestic wastewater:main sources and control method[J]. Environmental Science & Technology, 2009,43(24):9400-9406.

巩有奎, 王淑莹, 王莎莎, 等, 碳氮比对短程反硝化过程中N₂O产生的影响[J]. 化工学报, 2011, 62(7):2049-2054.

APHA(American Public Health Association). Standard Methods for the Examination of Water and Wastewater[S]. Baltimore:Port City Press, 1998.

OEHMEN A, KELLER-LEHMANN B, ZENG R J, et al. Optimisation of poly-beta-hydroxyalkanoate analysis using gas chromatography for enhanced biological phosphorus removal systems[J]. Journal of Chromatography A, 2005, 1070(1/2):131-136.

OEHMEN A, ZENG R J, YUAN Z G, et al. Anaerobic metabolism of propionate by polyphosphate-accumulating organisms in enhanced biological phosphorus removal systems[J]. Biotechnology and Bioengineering, 2005, 91(1):43-53.

贾淑媛, 王淑莹, 赵骥等, 驯化后的聚糖菌对NO₂^--N和NO₃^--N内源反硝化速率的影响[J].化工学报, 2017,68(12):4731-4738.

RIBERA-GUARDIA A, MARQUES R, ARANGIO C, et al. Distinctive denitrifying capabilities lead to differences in N₂O production by denitrifying polyphosphate accumulating organisms and denitrifying glycogen accumulating organisms[J]. Bioresource Technology,2016,219:106-113.

张建华, 彭永臻, 张淼,等. 不同电子受体配比对反硝化除磷特性及内碳源转化利用的影响[J].化工学报,2015,66(12):5045-5053

崔有为,金常林,王好韩,等. 碳源对O/A-F/F模式积累内源聚合物及反硝化的影响[J].环境科学,2019,40(1):336-342

WANG Y, GENG J, GUO G, et al. N₂O production in anaerobic/anoxic denitrifying phosphorus removal process:the effects of carbon sources shock[J]. Chemical Engineering Journal,2011,172(2/3):999-1007

ZHU X, CHEN Y. Reduction of N₂O and NO generation in anaerobic-aerobic (low dissolved oxygen) biological wastewater treatment process by using sludge alkaline fermentation liquid[J]. Environental Science & Technology, 2011,45:2137-2143

MIAO L, WANG S Y, LI B K, et al. Effect of carbon source type on intracellular stored polymers during endogenous denitritation (ED) treating landfill leachate[J]. Water Research, 2016, 100:405-412.

WEI Y, WANG S Y, MA B, et al. The effect of poly-b-hydroxyalkanoates degradation rate on nitrous oxide production in a denitrifying phosphorus removal system[J]. Bioresource Technology,2014, 170:175-182.

施引文献

资源附件(0)

访问统计