1株汞挥发真菌的分离及特性分析

司光正; 杨清晨; 董佳; 闫天歌; 常军军; 陈金全

doi:10.13205/j.hjgc.202009040

1株汞挥发真菌的分离及特性分析

doi: 10.13205/j.hjgc.202009040

司光正^1,2,
杨清晨²,
董佳¹,
闫天歌¹,
常军军¹,
陈金全^1,2, ,

1. 云南大学生态学与环境学院高原山地生态与退化环境修复重点实验室, 昆明 650091;
2. 云南大学国际河流与生态安全研究院, 昆明 650091

基金项目:

云南大学生态学与环境学院高原山地生态与退化环境修复重点实验室开放基金（2018DG005）；云南省教育厅基金（2019J0014）。

详细信息

作者简介:
司光正(1996-),男,硕士,主要研究方向为微生物治理土壤重金属污染。1203825109@qq.com

通讯作者:
陈金全(1979-),男,博士,副研究员,主要研究方向为微生物治理土壤重金属污染。chengjinquan@ynu.edu.cn

计量
- 文章访问数: 379
- HTML全文浏览量: 85
- PDF下载量: 4
- 被引次数: 0
出版历程
- 收稿日期: 2019-07-09

ISOLATION AND CHARACTERIZATION OF A STAIN OF MERCURY VOLATIZING FUNGUS

1. Key Laboratory of Plateau Mountain Ecology and Degraded Environment Rehabilitation, College of Ecology and Environment, Yunnan University, Kunming 650091, China;
2. Institute of International Rivers and Eco-Security, Yunnan University, Kunming 650091, China

摘要

摘要: 近年来土壤汞污染日益严峻，严重危害环境和人类健康，已成为一个世界性的环境问题，因此选择合理有效的方法对其进行修复与控制刻不容缓。有些微生物对汞具有吸附和挥发的特性，可用于受汞污染环境的修复。实验从受重金属污染的土壤中分离纯化出1株高抗汞真菌（DC-F9），其汞的最小抑菌浓度（MIC）达到160 mg/L；通过ITS序列比较，鉴别该菌属于曲霉属真菌；摇瓶培养发现，该真菌在Hg（Ⅱ）浓度为5 mg/L的培养基中对汞的挥发率、吸附率和总去除率分别为36.8%、58.4%、95.2%；而在Hg（Ⅱ）浓度为10 mg/L的培养基中，其值分别为45.4%、40.2%、85.6%；通过FTIR分析，发现该菌株为响应汞的胁迫表现出多种生理上变化。结果表明该菌株具有对汞污染环境修复的应用潜力。
- 土壤汞污染 /
- 汞挥发 /
- 真菌 /
- 吸附
Abstract: In recent years, soil contaminated with mercury (Hg) has become serious problem on earth, endangering the environment and human health. Therefore, it is urgent to develop a reasonable and effective method to remediate it. Some microorganisms have capacity of adsorption and volatilization for Hg(Ⅱ), and can be applied to the bioremediation of Hg-contaminated environments. In this study, a highly Hg-resistant fungus (DC-F9) was isolated from the heavy metal-contaminated soil with a minimum inhibitory concentration (MIC) of 160 mg/L. The strain belonged to the genus Aspergillus by the phylogenetic analysis of its ITS sequence. In the medium supplemented with Hg(Ⅱ) concentration of 5 mg/L, the volatilization rate, adsorption rate and total removal rate of Hg(Ⅱ) were 36.8%, 58.4%, 95.2% respectively; and they were 45.4%, 40.2%, 85.6% respectively at Hg(Ⅱ) concentration of 10 mg/L. The strain exhibited a variety of physiological changes in response to mercury stress by FTIR analyses. These results showed that the DC-F9 had potentials of bioremediation for Hg-contaminated environment.
- Hg-contaminated soil /
- volatilization /
- fungi /
- adsorption

HTML全文

参考文献(29)

NASCIMENTO A M A, CHARTONE-SOUZA E. Operon mer:bacterial resistance to mercury and potential for bioremediation of contaminated environments[J]. Genetics and Molecular Research, 2003, 2(1):92-101.

CHIEN M, NAKAHATA R, ONO T, et al. Mercury removal and recovery by immobilized Bacillus megaterium MB1[J]. Frontiers of Chemical Science and Engineering, 2012, 6(2):192-197.

ZAHIR F, RIZWI S J, HAQ S K, et al. Low dose mercury toxicity and human health[J]. Environmental Toxicology and Pharmacology, 2005, 20(2):351-360.

LI P, FENG X B, QIU G L, et al. Mercury pollution in Asia:a review of the contaminated sites[J]. Journal of Hazardous Materials, 2009, 168(2/3):591-601.

MAHBUB K R, KRISHNAN K, MEGHARAJ M, et al. Bioremediation potential of a highly mercury resistant bacterial strain Sphingobium SA2 isolated from contaminated soil[J]. Chemosphere, 2016, 144:330-337.

WAGNER-DOBLER I. Bioremediation of Mercury:Current Research and Industrial Applications[M]. Poole:Caister Academic Press, 2013:2-6.

王新, 周启星. 重金属与土壤微生物的相互作用及污染土壤修复[J]. 环境工程学报, 2004, 5(11), 1-5.

刘平, 仇广乐, 商立海. 汞污染土壤植物修复技术研究进展[J]. 生态学杂志, 2007, 26(6):933-937.

VELÁSQUEZ-RIAÑO M, BENAVIDES-OTAYA H D. Bioremediation techniques applied to aqueous media contaminated with mercury[J]. Critical Reviews in Biotechnology, 2016, 36(6):1124-1130.

邹鲤岭, 杨加庆, 程先锋, 等. 云南土壤和白菜重金属东川小江沿岸农田污染研究[J]. 西南农业学报, 2018, 31(4):754-758.

BLOOM N, FITZGERALD W F. Determination of volatile mercury species at the picogram level by low-temperature gas chromatography with cold-vapour atomic fluorescence detection[J]. Analytica Chimica Acta, 1988, 208(1/2):151-161.

WU D, ZHANG Z P, GAO Q L, et al. Isolation and characterization of aerobic, culturable, arsenic-tolerant bacteria from lead-zinc mine tailing in southern China[J]. World Journal of Microbiology and Biotechnology, 2018, 34(12):177.

卢福芝, 李启虔, 何海燕, 等. 一株抗镉真菌的分离鉴定及特性研究[J]. 环境工程, 2016, 34(4):64-67.

姜雨萌, 牛永春, 邓晖. rDNA ITS序列在ACCC真菌鉴定中的应用[J]. 微生物学通报, 2016, 43(5):942-947.

BELLEMAIN E, CARLSEN T, BROCHMANN C, et al. ITS as an environmental DNA barcode for fungi:an in silico approach reveals potential PCR biases[J]. BMC Microbiology, 2010, 10(1):189.

FRANÇOIS F, LOMBARD C, GUIGNER J M, et al. Isolation and characterization of environmental bacteria capable of extracellular biosorption of mercury[J]. Applied and Environmental Microbiology, 2012, 78(4):1097-1106.

SENEVIRATNE M, GUNARATNE S, BANDARA T, et al. Plant growth promotion by Bradyrhizobium japonicum under heavy metal stress[J]. South African Journal of Botany, 2016, 105:19-24.

KURNIATI E, ARFARITA N, IMAI T, et al. Potential bioremediation of mercury-contaminated substrate using filamentous fungi isolated from forest soil[J]. Journal of Environmental Sciences, 2014, 26(6):1223-1231.

ZAFAR S, AQIL F, AHMAD I. Metal tolerance and biosorption potential of filamentous fungi isolated from metal contaminated agricultural soil[J]. Bioresource Technology, 2007, 98(13):2557-2561.

韩桂云, 齐玉臣, 刘忱, 等. 温度、pH对菌根真菌生长影响的研究[J]. 生态学杂志, 1993,12(1):15-19.

魏景超. 真菌鉴定手册[M]. 上海:上海科学技术出版社,1979:498-499.

BARKAY T, MILLER S M, SUMMERS A O. Bacterial mercury resistance from atoms to ecosystems[J]. FEMS Microbiology Reviews, 2003, 27(2/3):355-384.

DASH H R, DAS S. Bioremediation of mercury and the importance of bacterial mer genes[J]. International Biodeterioration & Biodegradation, 2012, 75:207-213.

JIMÉNEZ-MORENO M, PERROT V, EPOV V N, et al. Chemical kinetic isotope fractionation of mercury during abiotic methylation of Hg (Ⅱ) by methylcobalamin in aqueous chloride media[J]. Chemical Geology, 2013, 336:26-36.

MARTÍNEZ-JUÁREZ V M, CÁRDENAS-GONZÁLEZ J F, TORRE-BOUSCOULET M E, et al. Biosorption of mercury (Ⅱ) from aqueous solutions onto fungal biomass[J]. Bioinorganic Chemistry and Applications, 2012:1-5.

OYETIBO G O, ISHOLA S T, IKEDA-OHTSUBO W, et al. Mercury bioremoval by Yarrowia strains isolated from sediments of mercury-polluted estuarine water[J]. Applied Microbiology and Biotechnology, 2015, 99(8):3651-3657.

OUST A, MØRETRØ T, KIRSCHNER C, et al. FT-IR spectroscopy for identification of closely related lactobacilli[J]. Journal of Microbiological Methods, 2004, 59(2):149-162.

黄晓婷, 马义丽, 李有志. 微紫青霉菌Penicillium janthinellum菌株GXCR吸附废水中Cd²⁺的研究[J]. 菌物学报, 2009, 28(6):850-856.

MECOZZI M, PIETROLETTI M, DI MENTO R. Application of FTIR spectroscopy in ecotoxicological studies supported by multivariate analysis and 2D correlation spectroscopy[J]. Vibrational Spectroscopy, 2007, 44(2):228-235.

施引文献

资源附件(0)

访问统计