1株汞挥发真菌的分离及特性分析

司光正; 杨清晨; 董佳; 闫天歌; 常军军; 陈金全

doi:10.13205/j.hjgc.202009040

1株汞挥发真菌的分离及特性分析

doi: 10.13205/j.hjgc.202009040

司光正^1,2,
杨清晨²,
董佳¹,
闫天歌¹,
常军军¹,
陈金全^1,2, ,

1. 云南大学生态学与环境学院高原山地生态与退化环境修复重点实验室, 昆明 650091;
2. 云南大学国际河流与生态安全研究院, 昆明 650091

基金项目:

云南大学生态学与环境学院高原山地生态与退化环境修复重点实验室开放基金（2018DG005）；云南省教育厅基金（2019J0014）。

详细信息

作者简介:
司光正(1996-),男,硕士,主要研究方向为微生物治理土壤重金属污染。1203825109@qq.com

通讯作者:
陈金全(1979-),男,博士,副研究员,主要研究方向为微生物治理土壤重金属污染。chengjinquan@ynu.edu.cn

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出版历程
- 收稿日期: 2019-07-09

ISOLATION AND CHARACTERIZATION OF A STAIN OF MERCURY VOLATIZING FUNGUS

1. Key Laboratory of Plateau Mountain Ecology and Degraded Environment Rehabilitation, College of Ecology and Environment, Yunnan University, Kunming 650091, China;
2. Institute of International Rivers and Eco-Security, Yunnan University, Kunming 650091, China

摘要

摘要: 近年来土壤汞污染日益严峻，严重危害环境和人类健康，已成为一个世界性的环境问题，因此选择合理有效的方法对其进行修复与控制刻不容缓。有些微生物对汞具有吸附和挥发的特性，可用于受汞污染环境的修复。实验从受重金属污染的土壤中分离纯化出1株高抗汞真菌（DC-F9），其汞的最小抑菌浓度（MIC）达到160 mg/L；通过ITS序列比较，鉴别该菌属于曲霉属真菌；摇瓶培养发现，该真菌在Hg（Ⅱ）浓度为5 mg/L的培养基中对汞的挥发率、吸附率和总去除率分别为36.8%、58.4%、95.2%；而在Hg（Ⅱ）浓度为10 mg/L的培养基中，其值分别为45.4%、40.2%、85.6%；通过FTIR分析，发现该菌株为响应汞的胁迫表现出多种生理上变化。结果表明该菌株具有对汞污染环境修复的应用潜力。
- 土壤汞污染 /
- 汞挥发 /
- 真菌 /
- 吸附
Abstract: In recent years, soil contaminated with mercury (Hg) has become serious problem on earth, endangering the environment and human health. Therefore, it is urgent to develop a reasonable and effective method to remediate it. Some microorganisms have capacity of adsorption and volatilization for Hg(Ⅱ), and can be applied to the bioremediation of Hg-contaminated environments. In this study, a highly Hg-resistant fungus (DC-F9) was isolated from the heavy metal-contaminated soil with a minimum inhibitory concentration (MIC) of 160 mg/L. The strain belonged to the genus Aspergillus by the phylogenetic analysis of its ITS sequence. In the medium supplemented with Hg(Ⅱ) concentration of 5 mg/L, the volatilization rate, adsorption rate and total removal rate of Hg(Ⅱ) were 36.8%, 58.4%, 95.2% respectively; and they were 45.4%, 40.2%, 85.6% respectively at Hg(Ⅱ) concentration of 10 mg/L. The strain exhibited a variety of physiological changes in response to mercury stress by FTIR analyses. These results showed that the DC-F9 had potentials of bioremediation for Hg-contaminated environment.
- Hg-contaminated soil /
- volatilization /
- fungi /
- adsorption

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