CSCD来源期刊
中国科技核心期刊
RCCSE中国核心学术期刊
JST China 收录期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

紫外线对再生水中斜生栅藻的生长抑制效果

李尚,  陶益,  刀国华,  江海莎,  周季,  余雯雯,  薛媛媚,  雍晓蕾,  何志明,  谢怀建,  付志敏,  胡洪营

downloadPDF
文章导航 > 环境工程  > 2020 >  38(10): 97-102,113
李尚, 陶益, 刀国华, 江海莎, 周季, 余雯雯, 薛媛媚, 雍晓蕾, 何志明, 谢怀建, 付志敏, 胡洪营. 紫外线对再生水中斜生栅藻的生长抑制效果[J]. 环境工程, 2020, 38(10): 97-102,113. doi: 10.13205/j.hjgc.202010015
引用本文: 李尚, 陶益, 刀国华, 江海莎, 周季, 余雯雯, 薛媛媚, 雍晓蕾, 何志明, 谢怀建, 付志敏, 胡洪营. 紫外线对再生水中斜生栅藻的生长抑制效果[J]. 环境工程, 2020, 38(10): 97-102,113. doi: 10.13205/j.hjgc.202010015
shu
LI Shang, TAO Yi, DAO Guo-Hua, JIANG Hai-Sha, Zhou-ji, YU Wen-Wen, XUE Yuan-Mei, YONG Xiao-Lei, HE Zhi-Ming, XIE Huai-Jian, FU Zhi-Min, HU Hong-ying. GROWTH SUPPRESSION EFFECT OF UV-C IRRADIATION ON SCENEDESMUS OBLIQUUS IN RECLAIMED WATER[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(10): 97-102,113. doi: 10.13205/j.hjgc.202010015
Citation: LI Shang, TAO Yi, DAO Guo-Hua, JIANG Hai-Sha, Zhou-ji, YU Wen-Wen, XUE Yuan-Mei, YONG Xiao-Lei, HE Zhi-Ming, XIE Huai-Jian, FU Zhi-Min, HU Hong-ying. GROWTH SUPPRESSION EFFECT OF UV-C IRRADIATION ON SCENEDESMUS OBLIQUUS IN RECLAIMED WATER[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(10): 97-102,113. doi: 10.13205/j.hjgc.202010015
shu

紫外线对再生水中斜生栅藻的生长抑制效果

doi: 10.13205/j.hjgc.202010015

  • 1. 清华大学 清华-伯克利深圳学院, 深圳 518055;

  • 2. 清华大学 深圳国际研究生院, 广东 深圳 518055;

  • 3. 清华大学 环境学院, 北京 100084;

  • 4. 成都市兴蓉环境股份有限公司, 成都 610041;

  • 5. 佛山柯维光电股份有限公司, 广东 佛山 528518

基金项目: 

国家重点研发计划项目（2018YFC0406300）；国家科技重大专项水专项（2017ZX07202002）；北京市科技计划课题（Z181100005518001）；清华大学（环境学院）-成都市兴蓉环境股份有限公司水务先进技术联合研究中心研发课题。

详细信息
    作者简介:

    李尚(1994-),男,硕士研究生,主要研究方向为再生水景观利用的紫外线抑藻技术。lis17@mails.tsinghua.edu.cn

    通讯作者:

    胡洪营(1963-),男,博士,教授,从事再生水安全利用理论与技术、环境微生物和生物技术领域的研究。hyhu-edu@mail.tsinghua.edu.cn

GROWTH SUPPRESSION EFFECT OF UV-C IRRADIATION ON SCENEDESMUS OBLIQUUS IN RECLAIMED WATER

  • 1. Tsinghua-Berkeley Shenzhen Institute, Tsinghua University, Shenzhen 518055, China;

  • 2. Tsinghua Shenzhen International Graduate School, Tsinghua University, Shenzhen 518055, China;

  • 3. School of Environment, Tsinghua University, Beijing 100084, China;

  • 4. Chengdu Xingrong Environmental Co., Ltd, Chengdu 610041, China;

  • 5. Foshan Comwin Light & Electricity Co., Ltd, Foshan 528518, China

    摘要
  • 摘要: 再生水具有就近易得、水量大且稳定等突出优点，已成为景观水体的重要补给水源。但再生水中含有氮、磷等营养物质，景观回用过程中可能会加剧藻类水华爆发风险。在藻类生长的早期阶段，采用紫外线处理有可能将再生水中藻类生物量控制在低风险范围，从而预防再生水景观回用水体中发生水华。以深圳市某污水处理厂二级出水为再生水回用景观水体的典型样品，考察了紫外线（254 nm波段）对典型微藻斜生栅藻的生长抑制效果，采用流式细胞术、调制荧光技术等方法，研究了不同紫外剂量对斜生栅藻的生长抑制效果，以及对藻细胞膜完整性和光合活性的影响。结果表明：对于初始密度约为7×104个/mL的斜生栅藻，50~100 mJ/cm2的紫外线能够取得1~5 d的生长抑制期，150~200 mJ/cm2剂量能将生长抑制期延长至10 d。紫外线能迅速引起斜生栅藻光合活性下降并能逐渐引起部分藻细胞破裂，100~200 mJ/cm2剂量处理后10 d内膜损伤细胞比例达到16%~71%。可见，紫外线能够对再生水中的斜生栅藻取得显著的生长抑制效果，是具有应用前景的景观水体水华风险防控手段。
    Abstract: Reclaimed water has the outstanding advantages of easy to obtain, large quantity, stable and high resource availability, and is gradually becoming an important source for landscape water. However, reclaimed water contains nutrients such as nitrogen and phosphorus, therefore exacerbates the risk of algal blooms during landscape reuse. UV-C irradiation can suppress algal growth for several days in the early growth stage, thereby is proposed as an approach for preventing algal blooms. In this study, the secondary effluent from a sewage treatment plant in Shenzhen was taken as a typical sample of reclaimed water for landscape, and the growth suppression effect of UV-C (254 nm) on a typical Chlorophyta species, Scenedesmus obliquus were investigated. The effect of UV-C doses on growth characteristics, cell membrane integrity and photosynthetic activity of S. obliquus were assessed by a flow cytometry and a PHYTO-PAM chlorophyll fluorometer. The results showed that for S. obliquus, with an initial density of about 7×104 cells/mL, UV-C irradiation at 50~100 mJ/cm2 achieved a growth inhibition period of 1~5 d, and the growth inhibition period could be further extended to 10 days at UV-C doses of 150~200 mJ/cm2. UV-C irradiation caused marked decreases in photosynthetic activity of S. obliquus immediately and caused rupture of part of the cells gradually, and the percentage of membrane damaged cells increased to 16%~71% within 10 days after 100~200 mJ/cm2 UV-C treatment. In summary, UV-C could achieve significant suppression effect on S. obliquus growth, which was a promising method for preventing and controlling the bloom risk of S. obliquus in reclaimed water.
    • HTML全文
    • 参考文献(26)
  • 张培培,王成新,肖伟华,等. 我国中长期水环境安全战略体系构建[J]. 环境保护, 2018, 46(5):64-67.
    国家发展改革委, 住房城乡建设部."十三五"全国城镇污水处理及再生利用设施建设规划[R]. 2016.
    杨佳,胡洪营,李鑫. 再生水水质环境中典型水华藻的生长特性[J]. 环境科学, 2010, 31(1):76-81.
    JOO J C, AHN C H, LEE S, et al. Algal growth potential of Microcystis aeruginosa from reclaimed water[J]. Water Environment Research, 2016, 88(1):54-62.
    ÚBEDA B, GÁLVEZ J Á, MICHEL M, et al. Microalgae cultivation in urban wastewater:Coelastrum cf. pseudomicroporum as a novel carotenoid source and a potential microalgae harvesting tool[J]. Bioresource Technology, 2017, 228:210-217.
    杨纪超. 深圳污水处理厂紫外消毒的影响因素及改进对策[D]. 哈尔滨:哈尔滨工业大学, 2013.
    SAKAI H, OGUMA K, KATAYAMA H, et al. Effects of low-or medium-pressure ultraviolet lamp irradiation on Microcystis aeruginosa and Anabaena variabilis[J]. Water Research, 2007, 41(1):11-18.
    TAO Y, HOU D L, ZHOU T R, et al. UV-C suppression on hazardous metabolites in Microcystis aeruginosa:unsynchronized production of microcystins and odorous compounds at population and single-cell level[J]. Journal of Hazardous Materials, 2018, 359:281-289.
    ISERI Y, KAWABATA Z, SASAKI M. Development of a boat equipped with UV lamps for suppression of freshwater red tide in a reservoir[J]. Japanese Journal of Water Treatment Biology, 1993, 29(2):61-70.
    ISERI Y, NAKAMICHI M, MAEDA T. Development of a red tide control ship equipped with a UV irradiation system-preliminary and field experiments on harmful marine algae[C]//IEEE, 2004.
    宋关玲,李伟,丛超,等. 水体磷浓度对斜生栅藻生长影响的研究[J]. 安徽农业科学, 2007,35(35):11576-11577.
    余娅,汪慧贞,廖日红,等. 再生水补水水体中氮磷对斜生栅藻生长影响的研究[J]. 给水排水, 2009, 45(增刊1):79-82.
    TAO Y, ZHANG X H, AU D W, et al. The effects of sub-lethal UV-C irradiation on growth and cell integrity of cyanobacteria and green algae[J]. Chemosphere, 2010, 78(5):541-547.
    TAO Y, MAO X Z, HU J Y, et al. Mechanisms of photosynthetic inactivation on growth suppression of Microcystis aeruginosa under UV-C stress[J]. Chemosphere, 2013, 93(4):637-644.
    陈慧婷,陶益,朱佳. 藻细胞计数及死/活分析的流式细胞仪方法[J]. 深圳职业技术学院学报, 2013, 12(1):23-27.
    SCHREIBER U. Pulse-amplitude-modulation (PAM) fluorometry and saturation pulse method:an overview[J]. Advantages in Photosynthesis and Respairation, 2004, 19(2):279-319.
    OU H, GAO N, DENG Y, et al. Immediate and long-term impacts of UV-C irradiation on photosynthetic capacity, survival and microcystin-LR release risk of Microcystis aeruginosa[J]. Water Research, 2012, 46(4):1241-1250.
    LIU L, CHU X N, CHEN P Y, et al. Effects of water quality on inactivation and repair of Microcystis viridis and Tetraselmis suecica following medium-pressure UV irradiation[J]. Chemosphere, 2016, 163:209-216.
    刘玲静,陶益,雷中凯,等. UV-C辐照对延滞期6种微藻生长抑制效果[J]. 环境科学与技术, 2015, 38(7):1-5

    ,27.
    OU H, GAO N Y, DENG Y, et al. Inactivation and degradation of Microcystis aeruginosa by UV-C irradiation[J]. Chemosphere, 2011, 85(7): 1192-1198.
    HUANG W, LU Y, ZHANG J B, et al. Inhibition mechanism of Microcystis aeruginosa under UV-C irradiation[J]. Desalination and Water Treatment, 2016, 57(24): 11403-11410.
    HE J Z, OU H, CHEN J X, et al. Intrinsic mechanism of UV-C-induced inactivation of Microcystis aeruginosa: impairment on photosynthetic system[J]. Water, Air, & Soil Pollution, 2016, 227(3): 82.
    LI S, DAO G H, TAO Y, et al. The growth suppression effects of UV-C irradiation on Microcystis aeruginosa and Chlorella vulgaris under solo-culture and co-culture conditions in reclaimed water[J]. Science of the Total Environment, 2020, 713: 136374.
    PHUKAN T, SYIEM M B. Modulation of oxidant and antioxidant homeostasis in the cyanobacterium Nostoc muscorum Meg1 under UV-C radiation stress[J]. Aquatic Toxicology, 2019,213: 105228.
    PFENDLER S, ALAOUI-SOSSÉ B, ALAOUI-SOSSÉ L, et al. Effects of UV-C radiation on Chlorella vulgaris, a biofilm-forming alga[J]. Journal of Applied Phycology, 2018, 30(3): 1607-1616.
    WANG B L, WANG X, HU Y W, et al. The combined effects of UV-C radiation and H2O2 on Microcystis aeruginosa, a bloom-forming cyanobacterium[J]. Chemosphere, 2015, 141: 34-43.
    • 相关文章
    • 施引文献
  • 资源附件(0)
  • 加载中
WeChat 点击查看大图
计量
  • 文章访问数:  201
  • HTML全文浏览量:  25
  • PDF下载量:  4
  • 被引次数: 0
出版历程
  • 收稿日期:  2019-12-02

目录

    /

    返回文章
    返回

    期刊在线

    作者中心

    友情链接

    版权所有 ©《工业建筑》杂志社有限公司 京ICP备11033253号-1

    本系统由北京仁和汇智信息技术有限公司 设计开发   百度统计