马赛菌属<i>Massilia</i> <i>neuiana</i>异养硝化-好氧反硝化脱氮特征实验

李一依; 赵勇娇; 刘芳; 李雪洁; 赵鑫; 胡筱敏

doi:10.13205/j.hjgc.202010016

马赛菌属Massilia neuiana异养硝化-好氧反硝化脱氮特征实验

doi: 10.13205/j.hjgc.202010016

1. 东北大学资源与土木工程学院环境工程系, 沈阳 110819;
2. 沈阳环境科学研究院, 沈阳 110167;
3. 江苏理工学院化学与环境工程学院, 江苏常州 213001

基金项目:

水体污染控制与治理科技重大专项（2013ZX07202-010）；中央直属高校基本科研业务费项目（N170104021，N182508026）。

详细信息

作者简介:
李一依(1995-),女,硕士研究生,主要研究方向为水污染控制与环境规划。berncastel@163.com

通讯作者:
赵鑫(1982-),男,副教授。zhaoxin@mail.neu.edu.cn

计量
- 文章访问数: 338
- HTML全文浏览量: 48
- PDF下载量: 6
- 被引次数: 0
出版历程
- 收稿日期: 2019-08-28

ANALYSIS ON THE HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFICATION CHARACTERISTICS OF THE NEW SPECIES OF MASSILIA NEUIANA

1. Department of Environmental Engineering, School of Resources and Civil Engineering, Northeastern University, Shenyang 110819, China;
2. Shenyang Academy of Environmental Sciences, Shenyang 110167, China;
3. School of Chemical and Environmental Engineering, Jiangsu University of Technology, Changzhou 213001, China

摘要

摘要: 基于马赛菌属脱氮Massilia neuiana的模式菌株PTW21进行研究，探讨其异养硝化-好氧反硝化脱氮能力。菌株PTW21具有高效异养硝化和好氧反硝化能力，对NH₄⁺-N和NO₂^--N去除率均超过90%。同时，菌株PTW21具有同步硝化反硝化能力，且反硝化效率高于硝化效率，但当有NH₄⁺-N存在时，会优先利用NH₄⁺-N，再利用NO₂^--N，存在硝化-反硝化竞争抑制现象。同步硝化反硝化时，菌株PTW21可以去除95%以上的NH₄⁺-N和NO₂^--N。Massilia neuiana的研究丰富了异养硝化-好氧反硝化微生物的种类，也为该菌种在污水处理厂的生物强化应用提供了前期基础。
- 生物脱氮 /
- 异养硝化 /
- 好氧反硝化 /
- Massilia /
- 同步硝化反硝化
Abstract: In this study, the heterotrophic nitrification-aerobic denitrification characteristics of strain PTW21, a typical strain of a novel nitrogen removal species Massilia neuiana, was investigated. The high efficiencies of heterotrophic nitrification and aerobic denitrification by strain PTW21 were demonstrated, which were both obtained over 90%. A higher denitrification efficiency was obtained than its nitrification efficiency in the simultaneous nitrification and denitrification (SND) by strain PTW21. However, a nitrification-denitrification competitive inhibition was observed in the presence of NH₄⁺-N that ammonium was preferentially consumed by strain PTW21, followed by nitrate. Totally over 95% of ammonia and nitrate nitrogen were removed by strain PTW21 in the SND process. The study on Massilia neuiana enriched the variety of heterotrophic nitrification-aerobic denitrification microbe, and established the preliminary foundation of the strain for the bio-augmentation application in sewage treatment plants.
- biological nitrogen removal /
- heterotrophic nitrification /
- aerobic denitrification /
- Massilia /
- simultaneous nitrification and denitrification

HTML全文

参考文献(26)

国家统计局, 环境保护部. 中国环境统计年鉴(2016)[M]. 北京:中国统计出版社, 2017.

王亚炜, 杜向群, 郁达伟, 等. 温榆河氨氮污染控制措施的效果模拟[J]. 环境科学学报, 2013, 33(2):479-486.

XIA S Q, LI J Y, WANG R C. Nitrogen removal performance and microbial community structure dynamics response to carbon nitrogen ratio in a compact suspended carrier biofilm reactor[J]. Ecological Engineering, 2008, 32:256-262.

ZHANG J B,WU P X, HAO B, et al. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001[J]. Bioresource Technology, 2011, 102(21):9866-9869.

AHMED M B, ZHOU J L, NGO H H, et al. Progress in the biological and chemical treatment technologies for emerging contaminant removal from wastewater:a critical review[J]. Journal of Hazardous Materials, 2017, 323(Part A):274-298.

KHARDENAVIS A A, KAPLEY A, PUROHIT H J. Simultaneous nitrification and denitrification by diverse Diaphorobacter sp.[J]. Applied Microbiology and Biotechnology, 2007, 77(2):403-409.

赵诣. 三株异养硝化细菌的分离、特征及其对水产养殖废水脱氮作用研究[D]. 杭州:浙江大学, 2010.

CHEN P Z, LI J, LI Q X, et al. Simultaneous heterotrophic nitrification and aerobic denitrification by bacterium Rhodococcus sp. CPZ24[J]. Bioresource Technology, 2012, 116:266-270.

SUN Y L, LI A, ZHANG X N, et al. Regulation of dissolved oxygen from accumulated nitrite during the heterotrophic nitrification and aerobic denitrification of Pseudomonas stutzeri T13[J]. Applied Microbiology and Biotechnology, 2015, 99(7):3243-3248.

PATRICIA A M A, ROBERTSON L A, KUENEN J G. Nitrification and denitrification by Thiospharea pantotropha in aerobic chemostat cultures[J]. Fems Microbiology Ecology, 2010, 18(4):305-315.

王欢, 汪苹, 张海波. 一株戴尔福特菌的异养硝化与好氧反硝化性能研究[J]. 北京工商大学学报, 2008, 26(2):1-5.

周迎芹, 信欣, 姚力, 等. 一株高效异养硝化-好氧反硝化菌的分离鉴定及脱氮性能[J]. 环境工程学报, 2013, 7(10):4127-4132.

潘玉瑾, 刘芳, 孟爽, 等好氧反硝化菌P. chengduensis ZPQ2的筛选及其反硝化条件优化[J]. 环境工程, 2016, 34(1):41-46.

LI C N, YANG J S, WANG X, et al. Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a phosphate accumulating bacterium Pseudomonas stutzeri YG-24[J]. Bioresource Technology, 2015, 182:18-25.

KIM J K, PARK K J, CHO K S, et al. Aerobic nitrification-denitrification by heterotrophic Bacillus strains[J]. Bioresource Technology, 2005, 96:1897-1906.

刘健楠, 汪苹, 尹明锐, 等. 高效异养硝化-好氧反硝化菌株的分离鉴定与脱氮性能[J]. 北京工商大学学报, 2010, 28(2):18-23.

JOO H, HIRAI M, SHODA M. Characteristics of ammonium removal by heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis No.4[J]. Journal of Bioscience and Bioengineering, 2005, 100:184-191.

LIU Y X, WANG Y, LI Y, et al. Nitrogen removal characteristics of heterotrophic nitrification-aerobic denitrification by Alcaligenes faecalis C16[J]. Chinese Journal of Chemical Engineering, 2015, 23(5):827-834.

CHEN Q, NI J R. Heterotrophic nitrification-aerobic denitrification by novel isolated bacteria[J]. Journal of Industrial Microbiology and Biotechnology, 2011, 38:1305-1310.

ZHAO X, LI X J, QI N, et al. Massilia neuiana sp. nov., isolated from wet soil[J]. Internal Journal of Systematic and Evolutionary Microbiology, 2017, 67(12):4943-4947.

国家环保总局. 水和废水监测分析方法[M]. 4版.北京:中国环境科学, 2006.

胡洪营, 张旭, 黄霞,等. 环境工程原理[M]. 3版.北京:高等教育出版社, 2015:423-424.

邵基伦. Burkholderia菌异养硝化-好氧反硝化特性及其强化废水处理的研究[D]. 广州:暨南大学, 2015.

HE T X, LI Z L, SUN Q, et al. Heterotrophic nitrification and aerobic denitrification by Pseudomonas tolaasii Y-11 without nitrite accumulation during nitrogen conversion[J]. Bioresource Technology, 2016, 200:493-499.

陈茂霞. 异氧硝化-好氧反硝化菌的筛选及其特性研究[D]. 成都:成都理工大学, 2012.

李紫惠. 异养硝化-好氧反硝化菌XH02的脱氮特性及强化脱氮过程中菌群结构变化的研究[D]. 广州:暨南大学, 2016.

施引文献

资源附件(0)

访问统计