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利用磷脂脂肪酸(PLFAs)生物标记法分析人工湿地根际土壤微生物多样性

孙和泰,  华伟,  祁建民,  黄治军,  杜存浩,  武晗琪,  倪利晓

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文章导航 > 环境工程  > 2020 >  38(11): 103-109
孙和泰, 华伟, 祁建民, 黄治军, 杜存浩, 武晗琪, 倪利晓. 利用磷脂脂肪酸(PLFAs)生物标记法分析人工湿地根际土壤微生物多样性[J]. 环境工程, 2020, 38(11): 103-109. doi: 10.13205/j.hjgc.202011017
引用本文: 孙和泰, 华伟, 祁建民, 黄治军, 杜存浩, 武晗琪, 倪利晓. 利用磷脂脂肪酸(PLFAs)生物标记法分析人工湿地根际土壤微生物多样性[J]. 环境工程, 2020, 38(11): 103-109. doi: 10.13205/j.hjgc.202011017
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SUN He-tai, HUA Wei, QI Jian-min, HUANG Zhi-jun, DU Cun-hao, WU Han-qi, NI Li-xiao. ANALYSIS ON MICROBIAL DIVERSITY IN THE RHIZOSPHERE OF CONSTRUCTED WETLANDS BY PHOSPHOLIPID FATTY ACIDS BIOMARKERS[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(11): 103-109. doi: 10.13205/j.hjgc.202011017
Citation: SUN He-tai, HUA Wei, QI Jian-min, HUANG Zhi-jun, DU Cun-hao, WU Han-qi, NI Li-xiao. ANALYSIS ON MICROBIAL DIVERSITY IN THE RHIZOSPHERE OF CONSTRUCTED WETLANDS BY PHOSPHOLIPID FATTY ACIDS BIOMARKERS[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(11): 103-109. doi: 10.13205/j.hjgc.202011017
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利用磷脂脂肪酸(PLFAs)生物标记法分析人工湿地根际土壤微生物多样性

doi: 10.13205/j.hjgc.202011017

  • 1. 江苏方天电力技术有限公司, 南京 211102;

  • 2. 河海大学 教育部浅水湖泊重点实验室, 南京 210098

基金项目: 

国家自然科学基金（51779079）；江苏省自然科学基金（BK20181313）；江苏省水利科技项目（2018037）；江苏方天电力项目（819049016）。

详细信息
    作者简介:

    孙和泰(1972-),男,硕士研究生,高级工程师,主要研究方向为人工湿地处理技术和能源动力。nuojin@sina.com

    通讯作者:

    倪利晓(1973-),女,教授,主要研究方向为蓝藻水华控制技术与机理、污染物迁移转化及人工湿地处理技术等。nilixiao@hhu.edu.cn

ANALYSIS ON MICROBIAL DIVERSITY IN THE RHIZOSPHERE OF CONSTRUCTED WETLANDS BY PHOSPHOLIPID FATTY ACIDS BIOMARKERS

  • 1. Jiangsu Fangtian Electric Power Technology Co., Ltd, Nanjing 211102, China;

  • 2. Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, China

    摘要
  • 摘要: 采用磷脂脂肪酸（PLFAs）生物标记法，分析了4组人工湿地分别栽种2种植物情况下植物根际土壤的微生物群落结构。结果表明：4组湿地植物根际土壤中PLFAs总量为1322~2769 μg/g，PLFAs种类为18~30，湿地植物根际土壤中PLFAs主要包含饱和脂肪酸和单不饱和脂肪酸；植物根际土壤中细菌含量最高，其中好养菌含量显著高于厌氧菌。对PLFAs总量和湿地设计运行参数进行Pearson相关分析，PLFAs含量与基质孔隙率和进水水质呈显著正相关。
    Abstract: The microbial community structure of rhizosphere soils in four groups of constructed wetlands were analyzed by phospholipid fatty acid (PLFAs) biomarker method. The results showed that the total amount of phospholipid fatty acid biomarker (PLFAs) in rhizosphere soil of four groups varied from 1322 μg/g to 2769 μg/g, and the range of PLFAs sorts number varied from 18 to 30. PLFAs in wetland plant rhizosphere soil mainly contained saturated fatty acids and monounsaturated fatty acids. The content of bacteria in plant rhizosphere soil was the highest, and the content of well-nourished bacteria was significantly higher than that of anaerobes. The total amount of PLFAs and the design and operation parameters of wetland were analyzed by Pearson correlation analysis. The results showed that the content of PLFAs was positively correlated with matrix porosity and influent water quality.
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出版历程
  • 收稿日期:  2019-12-17
  • 网络出版日期:  2021-04-23
  • 刊出日期:  2021-04-23

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