氮、磷浓度对伪鱼腥藻生长及光合生理的影响

周利; 王茂伟; 高静思; 朱佳

doi:10.13205/j.hjgc.202011018

氮、磷浓度对伪鱼腥藻生长及光合生理的影响

doi: 10.13205/j.hjgc.202011018

周利¹,
王茂伟¹,
高静思^2, ,,
朱佳³

1. 青岛理工大学环境与市政工程学院, 山东青岛 266033;
2. 哈尔滨工业大学深圳研究生院, 广东深圳 518055;
3. 深圳职业技术学院建筑与环境工程学院, 广东深圳 518055

基金项目:

国家水利部公益性行业科研专项（201301047）。

详细信息

作者简介:
周利(1963-),男,博士,教授,主要研究方向为水污染控制与污水资源化研究。qtechzhouli@163.com

通讯作者:
高静思(1984-),女,博士研究生,主要研究方向为水污染治理与水体修复。123486419@qq.com

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出版历程
- 收稿日期: 2019-08-14
- 网络出版日期: 2021-04-23
- 刊出日期: 2021-04-23

EFFECTS OF NITROGEN AND PHOSPHORUS CONCENTRATIONS ON GROWTH AND PHOTOSYNTHETIC PHYSIOLOGY OF Pseudanabaena sp.

1. School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China;
2. Harbin Institute of Technology Shenzhen Graduate School, Shenzhen 518055, China;
3. Department of Building and Environmental Engineering, Shenzhen Polytechnic, Shenzhen 518055, China

摘要

摘要: 以伪鱼腥藻（Pseudanabaena sp.）为研究对象，研究了不同氮、磷浓度对伪鱼腥藻生长、叶绿素荧光参数（F_v/F_m、α、rETR_max、I_K）及类胡萝卜素与叶绿素a比值等的影响，并应用Monod和Droop方程考察氮、磷营养盐对伪鱼腥藻生长动力学的影响，分别计算得到伪鱼腥藻对总磷的半饱和常数K_{s_P}，细胞内磷份额Q₀与对总氮的半饱和常数K_{s_N}。结果表明：氮、磷浓度对伪鱼腥藻的生长影响极显著（P<0.01），在氮浓度为0~230.4 mg/L内，伪鱼腥藻的生物量与氮浓度呈正相关，磷浓度>1.28 mg/L会抑制伪鱼腥藻生长。氮浓度对各叶绿素荧光参数有显著影响（P<0.05），磷浓度对F_v/F_m、I_K有显著影响（P<0.05），而对rETR_max、α影响不显著（P>0.05）。K_{s_P}≪K_{s_N}，伪鱼腥藻对磷具有更好的亲和性，伪鱼腥藻能够正常生长的细胞最小内磷份额Q₀为0.0486 μg/mg，低于该值则藻细胞的增殖停止。类胡萝卜素与叶绿素a比值与氮、磷营养盐浓度呈负相关。
- 伪鱼腥藻 /
- 氮 /
- 磷 /
- 生长动力学 /
- 叶绿素荧光参数
Abstract: The effects of nitrogen and phosphorus concentrations on the growth, chlorophyll fluorescence parameters (F_v/F_m、α、rETR_max、I_K) and the ratio of carotenoid to chlorophyll a of Pseudanabaena sp. were investigated. Moreover, the Pseudanabaena sp. growth kinetics utilizing nitrogen and phosphorus was demonstrated through Monod equation and Droop equation. The semi-saturation constants, K_{s_P} of total phosphorus, intracellular phosphorus share and the semi-saturation constants K_{s_N} of total nitrogen were calculated. The results showed that the effects of different nitrogen and phosphorus concentrations on the growth of Pseudanabaena sp. were significant(P<0.01). The chlorophyll a of Pseudanabaena sp. was positively correlated with nitrogen concentration ranging from 0 to 230.4 mg/L, and phosphorus concentration higher than 5.12 mg/L inhibited the growth of Pseudanabaena sp. The chlorophyll fluorescence parameters were significantly affected by different nitrogen concentrations (P<0.05). Concentrations of phosphorus had a significant effect on F_v/F_m、I_K and no significant effect on rETR_max, α (P>0.05). K_{s_P} was far less than K_{s_N}, which proved that Pseudanabaena sp. had better affinity for phosphorus. The minimum share of intracellular phosphorus for Pseudanabaena sp. was 0.0486 μg/mg, under which the proliferation of algal cells will cease. The ratio of carotenoid to chlorophyll a was negatively correlated with the concentration of nitrogen and phosphorus.
- Pseudanabaena sp. /
- nitrogen /
- phosphorus /
- growth kinetics /
- chlorophyll fluorescence parameters

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参考文献(31)

金相灿.中国湖泊富营养化控制技术.中国湖泊富营养化及其防治国际学术研讨会专家论文集[C]//北京:国家环境保护总局,2000:215-223.

PAN G, ZHANG M M, CHEN H,et al.Removal of cyanobacterial blooms in Taihu Lake using local soils. I. Equilibrium and kinetic screening on the flocculation of Microcystis aeruginosa using commercially available clays and minerals[J]. Environmental Pollution,2006,141(2):195-200.

赵静静,张方可,高静思,等.梯级水库输水线路伪鱼腥藻属时空分布特征及影响因子分析[J].水资源保护, 2016, 32(3):111-116.

KAKIMOTO M,ISHIKAWA T,MIYAGI A.Culture temperature affects gene expression and metabolic pathways in the 2-methylisoborneol-producing cyanobacterium Pseudanabaena galeat[J].Journal of Plant Physiology,2014,171(3/4):292-300.

JANG M H,HA K,LUCAS M C.Changes in microcystin production by Microcystis aeruginosa exposed to phytoplank-tivorous and omnivorous fish[J].Aquatic Toxicology, 2004, 68(1):51-59.

OUDRA B,LOUDIKI M,VASCONCELOS V.Detection and quantification of microcystins from cyano-bacterial strains isolated from reservoirs and ponds in Morocco[J].Environmental Toxicology, 2002, 17(1):32-39.

OH H M,LEE S J,JANG M H.Microcystin production by Microcystis aeruginosa in a phosphorus-limited chemostat[J].Applied and Environmental Microbiology,2000,66(6):176-179.

王英英.不同氮磷质量浓度对太浦河四种优势藻类生长影响的研究[D].上海:上海师范大学,2016.

DAUTANIA G K,SINGH G P,DAUTANIA G K,et al.Role of light and dark cycle and different temperature in the regulation of growth and protein expression in oscillatoria agardhii strain[J].Brazilian Archives of Biology & Technology,2014,57(6):933-940.

张艳,马放,李圭白.不同生长条件对铜绿微囊藻生长的影响[J].净水技术,2014,33(3):84-86

,99.

陈建中,刘志礼,李晓明,等.温度、pH和氮、磷含量对铜绿微囊藻(Microcystis aeruginosa)生长的影响[J].海洋与湖沼, 2010, 41(5):714-718.

HECKY R E, P KILHAM. Nutrient limitation of phytoplankton in freshwater and marine environments:a review of recent evidence on the effects of enrichment[J]. Limnology and Oceanography,1988, 33(4):796-822.

YANG Z J,LIU D F.Influence of the impounding process of the Three Gorges Reservoir up to water level 172.5m on water eutrophication in the Xiangxi Bay[J].Technological Sciences,2010,53(4):1114-1125.

张玮,林一群,郭定芳,等.不同氮、磷浓度对铜绿微囊藻生长、光合及产毒的影响[J].水生生物学报, 2006, 30(3):318-322.

朱嘉成,周利,朱佳,等.水生观赏植物红掌对铜绿微囊藻的化感作用[J].环境科学研究,2015, 28(10):1638-1644.

张自杰, 周帆. 活性污泥反应动力学[M]. 北京:中国环境科学出版社,1989.

DROOP M R.VITAMIN B12 and marine ecology:Ⅳ.The kinetics of uptake,growth,and inhibition in monochrysis luther[J]. Journal of the Marine Biological Association of the United Kingdom,1986, 48(3):689-733.

KUSTER A,SCHAIBLE R, SCHUBERT H.Light acclimation of photosynthesis In three charophyte species[J].Aquatic Botany, 2004, 79:111-124.

易文利,金相灿,储昭升,等.不同质量浓度的磷对铜绿微囊藻生长及细胞内磷的影响[J].环境科学研究,2004,17(增刊1):58-61.

LEVY I,GANTT E.Development of photosynthetic activity in Por-phyridium purpureum (Rhodophyta) following nitrogen starvation[J]. Journal of Phycology,1990,26:62-68.

HUDOSN J J, TAYLON W D, SCHINDLER D W.Phosphate concertrations in lakes[J]. Nat,2000,406(6):54-56.

GEIDER R J, MACINTYRE H L, GRAZIANO L M, et al. Responses of the photosynthetic apparatus of Dunaliella tertiolecta (Chlorophyceae) to nitrogen and phosphorus limitation[J].European Journal of Phycology, 1998, 33(4):315-332.

张永奎, 温皓程,王立柱,等. 不同氮源、无机碳源对小球藻生长及油脂积累的影响[R]. 江苏常州:全国生物化工技术发展探讨会,2010.

LIPPEMEIER S, KLAUSH R H, HAR TIG V P, et al. In-line recording o f PAM fluorescence of phytoplankton cultures as a new tool for studying effects o f f luctuating nutrient supply on photo synthesis[J]. European Journal of Phycology, 2001,36:89-100.

赵先富,马沛明,刘国祥,等.不同磷浓度对小球藻铜绿微囊藻生长及生理的影响[J].环境科学与技术,2013,36(11):1-5.

LIU J L,WANG J F,LIU T Z, et al. The effects of nitrogen starvation on lipid accumulation and photosynthesis of Scenedesmus dimorphus[J].Marine Sciences, 2013,37(7):13.

ÖRDÖG V,STIRK W A,BÁLINT P, et al. Changes in lipid,protein and pigment concentrations in nitrogen-stressed Chlorella minutissima cultures[J].Journal of Applied Phycology,2012,24(4):907-914.

汪亚俊,孙明哲,李爱芬,等.不同氮素水平对产油尖状栅藻生长及光合生理的影响[J].中国生物工程杂志,2014,34(12):51-58.

YOUNG E B,BEARDALL J. Rapid ammonium and nitrate-induced perturbations to Chl-a flurescence in nitrogen-stressed Dunaliella tertiolecata(Chlorophyta)[J].Journal of Phycology,2003,39:332-342.

范丽敏,梁英,田传远,等.营养盐浓度对棕鞭藻叶绿素荧光参数、细胞密度和总脂含量的影响[J].水产科学,2012,31(5):249-254.

陈书秀,梁英,王虎,等.盐度对盐生杜氏藻叶绿素荧光参数的影响[J].科技创新导报,2010(23):4-5,7.

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