氮、磷浓度对伪鱼腥藻生长及光合生理的影响

周利; 王茂伟; 高静思; 朱佳

doi:10.13205/j.hjgc.202011018

氮、磷浓度对伪鱼腥藻生长及光合生理的影响

doi: 10.13205/j.hjgc.202011018

周利¹,
王茂伟¹,
高静思^2, ,,
朱佳³

1. 青岛理工大学环境与市政工程学院, 山东青岛 266033;
2. 哈尔滨工业大学深圳研究生院, 广东深圳 518055;
3. 深圳职业技术学院建筑与环境工程学院, 广东深圳 518055

基金项目:

国家水利部公益性行业科研专项（201301047）。

详细信息

作者简介:
周利(1963-),男,博士,教授,主要研究方向为水污染控制与污水资源化研究。qtechzhouli@163.com

通讯作者:
高静思(1984-),女,博士研究生,主要研究方向为水污染治理与水体修复。123486419@qq.com

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出版历程
- 收稿日期: 2019-08-14
- 网络出版日期: 2021-04-23
- 刊出日期: 2021-04-23

EFFECTS OF NITROGEN AND PHOSPHORUS CONCENTRATIONS ON GROWTH AND PHOTOSYNTHETIC PHYSIOLOGY OF Pseudanabaena sp.

1. School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China;
2. Harbin Institute of Technology Shenzhen Graduate School, Shenzhen 518055, China;
3. Department of Building and Environmental Engineering, Shenzhen Polytechnic, Shenzhen 518055, China

摘要

摘要: 以伪鱼腥藻（Pseudanabaena sp.）为研究对象，研究了不同氮、磷浓度对伪鱼腥藻生长、叶绿素荧光参数（F_v/F_m、α、rETR_max、I_K）及类胡萝卜素与叶绿素a比值等的影响，并应用Monod和Droop方程考察氮、磷营养盐对伪鱼腥藻生长动力学的影响，分别计算得到伪鱼腥藻对总磷的半饱和常数K_{s_P}，细胞内磷份额Q₀与对总氮的半饱和常数K_{s_N}。结果表明：氮、磷浓度对伪鱼腥藻的生长影响极显著（P<0.01），在氮浓度为0~230.4 mg/L内，伪鱼腥藻的生物量与氮浓度呈正相关，磷浓度>1.28 mg/L会抑制伪鱼腥藻生长。氮浓度对各叶绿素荧光参数有显著影响（P<0.05），磷浓度对F_v/F_m、I_K有显著影响（P<0.05），而对rETR_max、α影响不显著（P>0.05）。K_{s_P}≪K_{s_N}，伪鱼腥藻对磷具有更好的亲和性，伪鱼腥藻能够正常生长的细胞最小内磷份额Q₀为0.0486 μg/mg，低于该值则藻细胞的增殖停止。类胡萝卜素与叶绿素a比值与氮、磷营养盐浓度呈负相关。
- 伪鱼腥藻 /
- 氮 /
- 磷 /
- 生长动力学 /
- 叶绿素荧光参数
Abstract: The effects of nitrogen and phosphorus concentrations on the growth, chlorophyll fluorescence parameters (F_v/F_m、α、rETR_max、I_K) and the ratio of carotenoid to chlorophyll a of Pseudanabaena sp. were investigated. Moreover, the Pseudanabaena sp. growth kinetics utilizing nitrogen and phosphorus was demonstrated through Monod equation and Droop equation. The semi-saturation constants, K_{s_P} of total phosphorus, intracellular phosphorus share and the semi-saturation constants K_{s_N} of total nitrogen were calculated. The results showed that the effects of different nitrogen and phosphorus concentrations on the growth of Pseudanabaena sp. were significant(P<0.01). The chlorophyll a of Pseudanabaena sp. was positively correlated with nitrogen concentration ranging from 0 to 230.4 mg/L, and phosphorus concentration higher than 5.12 mg/L inhibited the growth of Pseudanabaena sp. The chlorophyll fluorescence parameters were significantly affected by different nitrogen concentrations (P<0.05). Concentrations of phosphorus had a significant effect on F_v/F_m、I_K and no significant effect on rETR_max, α (P>0.05). K_{s_P} was far less than K_{s_N}, which proved that Pseudanabaena sp. had better affinity for phosphorus. The minimum share of intracellular phosphorus for Pseudanabaena sp. was 0.0486 μg/mg, under which the proliferation of algal cells will cease. The ratio of carotenoid to chlorophyll a was negatively correlated with the concentration of nitrogen and phosphorus.
- Pseudanabaena sp. /
- nitrogen /
- phosphorus /
- growth kinetics /
- chlorophyll fluorescence parameters

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