EFFECTS OF LOW TEMPERATURE ON PARTIAL NITRIFICATION GRANULAR SYSTEM
-
摘要: 考察了温度变化对亚硝化颗粒污泥反应器的长期和短期影响特性,结果表明:在进水ρ(NH4+-N)为(35.8±5.2)mg/L、水力停留时间为2.0 h以及运行温度为7~17℃的条件下,反应器保持着95%的亚硝化率和0.18~0.25 kg/(m3·L)的NH4+-N去除负荷;反应器中较低的ρ(DO)∶ρ(NH4+-N)(<0.25)是实现亚硝酸盐氧化细菌(NOB)有效抑制的关键因素;长期低温运行造成颗粒污泥比NH4+-N氧化速率(SAOR)从(237±14)g/(g·d)下降至(93±11)g/(g·d),但颗粒污泥中氨氧化细菌(AOB)的活化率(实际SAOR与最大SAOR之比)从48%升至约85%。批式实验结果表明,在7.1~28℃的短时温度变化内,亚硝化颗粒污泥NH4+-N氧化反应的温度系数(θ)和活化能(Ea)分别为1.042~1.063,29.7~41.9 kJ/mol,均显著低于同等条件下絮体污泥的数值,表明颗粒污泥AOB比絮体污泥AOB具有更好的抗温度冲击能力。该研究结果可为基于颗粒污泥的高效城市污水亚硝化技术提供参考。Abstract: The long-and short-term effects of temperature change on partial nitrification granules was investigated. The results showed that nitrite accumulation efficiency and ammonium removal loading rates of the granular reactor were stably maintained at above 95% and 0.18~0.25 kg/(m3·L), when treating the influent of (35.8±5.2) mg/L with hydraulic retention time of 2.0 h at 7~17 ℃. The low DO/NH4+-N ratio (<0.25) caused the efficient nitrite oxidizing bacteria (NOB) repression in the granular reactor. Though the lowering temperature caused the decrease of specific ammonia oxidation rates (SAOR) of partial nitrification granules from (237±14) g/(g·d) (at 17 ℃) to (93±11) g/(g·d) (at 8 ℃), the active AOB ratio (the ratio between actual SAOR and maximal SAOR of granules) increased from 48% to 85%. Batch tests results indicated that the temperature coefficient (θ) and activation energy (Ea) of the ammonia oxidizing bacteria (AOB) living in the granules was around 1.042~1.063, 29.7~41.9 kJ/mol respectively from 7.1 ℃ to 28 ℃, which were lower than that reported in flocculent sludge systems. These results suggested that the AOB living in the granules were less influenced by low temperatures than that living in the flocs. The research could provide practical support for the development of high-rate nitritation technology for municipal wastewater treatment with granular sludge.
-
Key words:
- granular sludge /
- nitritation /
- temperature effect /
- activation energy
-
WINKLER M K, STRAKA L. New directions in biological nitrogen removal and recovery from wastewater[J]. Current Opinion in Biotechnology, 2019, 57:50-55. PENG Y Z, ZHU G B. Biological nitrogen removal with nitrification and denitrification via nitrite pathway[J]. Applied Microbiology and Biotechnology, 2006, 73(1):15-26. ZHANG M, WU S, ZUO Z Q, et al. Predictions of the influent and operational conditions for partial nitritation with a model incorporating pH dynamics[J]. Environmental Science Technology, 2018, 52(11):6457-6465. 彭永臻, 李璐凯, 李夕耀, 等. 不同pH值及碱性物质对短程硝化的影响[J].北京工业大学学报,2017,43(10):1554-1562. WANG Q L, LIU Y, JIANG G M, et al. Side-stream sludge treatment using free nitrous acid selectively eliminates nitrite oxidizing bacteria and achieves the nitrite pathway[J]. Water Research,2014, 55:245-255. WANG Q L, DUAN H R,WEI W, et al. Achieving stable mainstream nitrogen removal via the nitrite pathway by sludge treatment using free ammonia[J]. Environmental Science Technology, 2017, 51(17):9800-9807. REGMI P, MILLER M W, HOLGATE B, et al. Control of aeration, aerobic SRT and COD input for mainstream nitritation/denitritation[J]. Water Research,2014, 57:162-171. 付昆明,廖敏辉,任奕, 等. 污水短程硝化影响因素的对比分析[J]. 中国给水排水,2019,35(4):24-29. WU J, HE C D, VAN LOOSDRECHT M C M, et al. Selection of ammonium oxidizing bacteria (AOB) over nitrite oxidizing bacteria (NOB) based on conversion rates[J]. Chemical Engineering Journal, 2016, 304:953-961. CAO Y S, VAN LOOSDRECHT M C M, DAIGGER G T. Mainstream partial nitritation-anammox in municipal wastewater treatment:status, bottlenecks, and further studies[J]. Applied Microbiology and Biotechnology, 2017, 101(4):1365-1383. LIU W R, YANG D H, SHEN Y L, et al. Two-stage partial nitritation-anammox process for high-rate mainstream deammonification[J]. Applied Microbiology and Biotechnology, 2018, 102(18):8079-8091. 刘文如, 杨殿海, 沈耀良, 等. 主流条件下两级式PN-ANAMMOX工艺的高效能脱氮过程[J]. 环境科学, 2018, 39(12):5580-5586. 赵青,卞伟, 李军, 等. DO/NH4+-N调控实现MBBR工艺生活污水短程硝化[J]. 中国环境科学,2017,37(12):4511-4517. BARTROLI A, PEREZ J, CARRERA J. Applying ratio control in a continuous granular reactor to achieve full nitritation under stable operating conditions[J]. Environmental Science Technology, 2010, 44(23):8930-8935. ISANTA E, REINO C, CARRERA J, et al. Stable partial nitritation for low-strength wastewater at low temperature in an aerobic granular reactor[J]. Water Research, 2015, 80:149-158. REINO C, SUAREZ-OJEDA M, PEREZ J, et al. Kinetic and microbiological characterization of aerobic granules performing partial nitritation of a low-strength wastewater at 10℃[J]. Water Research, 2016, 101:147-156. HWANG J H, OLESZKIEWICZ J A. Effect of cold-temperature shock on nitrification[J]. Water Environment Research, 2007, 79(9):964-968. GUO J H, PENG Y Z, HUANG H J, et al. Short-and long-term effects of temperature on partial nitrification in a sequencing batch reactor treating domestic wastewater[J]. Journal of Hazardous Materials, 2010, 179(1/2/3):471-479. DEGRAAF A A V, DEBRUIJN P, ROBERTSON L A, et al. Autotrophic growth of anaerobic ammonium-oxidizing micro-organisms in a fluidized bed reactor[J]. Microbiology-Uk, 1996, 142, 2187-2196. 国家环境保护总局, 水和废水监测编委会. 水和废水监测分析方法[M]. 4版. 北京:中国环境科学出版社, 2002. KIM J H, GUO X J, PARK H S. Comparison study of the effects of temperature and free ammonia concentration on nitrification and nitrite accumulation[J]. Process Biochemistry, 2008, 43(2):154-160. WANG J L, YANG N. Partial nitrification under limited dissolved oxygen conditions[J]. Process Biochemistry, 2004, 39(10): 1223-1229. HEAD M A, OLESZKIEWICZ J A. Bioaugmentation for nitrification at cold temperatures[J]. Water Research, 2004, 38(3): 523-530. MOGENS H, WILLI G, TAKASHI M, et al. Activated Sludge Models ASM1, ASM2, ASM2d and ASM3(Scientific and Technical Report No.9)[M]. London: IWA Publishing, 2000. SOZEN S, ORHON D, SAN H A. A new approach for the evaluation of the maximum specific growth rate in nitrification[J]. Water Research, 1996, 30(7): 1661-1669. OLESZKIEWICZ J A, BERQUIST S A. Low temperature nitrogen removal in sequencing batch reactors[J]. Water Research, 1988, 22(9): 1163-1171. SALVETTI R, AZZELLINO A, CANZIANI R, et al. Effects of temperature on tertiary nitrification in moving-bed biofilm reactors[J]. Water Research, 2006, 40(15): 2981-2993. BENYAHIA F, POLOMARKAKI R. Mass transfer and kinetic studies under no cell growth conditions in nitrification using alginate gel immobilized Nitrosomonas[J]. Process Biochemistry, 2005, 40(3/4): 1251-1262. PAINTER H A, LOVELESS J E. Effect of temperature and pH value on the growth-rate constants of nitrifying bacteria in the activated-sludge process[J]. Water Research, 1983, 17(3): 237-248. HALL E R, MURPHY K L. Sludge age and substrate effects on nitrification kinetics[J]. Journal Water Pollution Control Federation, 1985, 57(5): 413-418. RANDALL C W, BENEFIELD L D, BUTH D. The effects of temperature on the biochemical reaction rates of the activated sludge process[J]. Water Science and Technology, 1982, 14(1/2): 413-430. LIU W R, CHEN W J, YANG D H, et al. Functional and compositional characteristics of nitrifiers reveal the failure of achieving mainstream nitritation under limited oxygen or ammonia conditions[J]. Bioresource Technology, 2019, 275: 272-279. LIU W R, YANG D H. Evaluating the feasibility of ratio control strategy for achieving partial nitritation in a continuous floccular sludge reactor: experimental demonstration[J]. Bioresource Technology, 2017, 224: 94-100.
点击查看大图
计量
- 文章访问数: 110
- HTML全文浏览量: 22
- PDF下载量: 9
- 被引次数: 0