运行模式对同步短程硝化反硝化脱氮及微生物群落特征的影响

韩亚琳; 王福浩; 王群; 李婷; 佘宗莲

doi:10.13205/j.hjgc.202101007

运行模式对同步短程硝化反硝化脱氮及微生物群落特征的影响

doi: 10.13205/j.hjgc.202101007

1. 中国海洋大学海洋环境与生态教育部重点实验室, 山东青岛 266100;
2. 青岛水务环境公司, 山东青岛 266021

基金项目:

中央高校基本科研业务费专项（201964003）。

详细信息

作者简介:
韩亚琳(1993-),女,研究生,主要研究方向为污水生物脱氮处理。hanyalin6@163.com

通讯作者:
佘宗莲(1962-),女,教授,硕士生导师,主要研究方向为污水生物处理与水污染控制。szlszl@ouc.edu.cn

计量
- 文章访问数: 266
- HTML全文浏览量: 39
- PDF下载量: 16
- 被引次数: 0
出版历程
- 收稿日期: 2019-09-07
- 网络出版日期: 2021-04-23

EFFECT OF OPERATIONAL MODE ON NITROGEN REMOVAL AND MICROBIAL CUMMUNITY IN PROCESS OF SIMULTANEOUS PARTIAL NITRIFICATION AND DENITRIFICATION

1. Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China;
2. Qingdao Water Group Corporation Limited, Qingdao 266021, China

摘要

摘要: 采用复合序批式生物膜反应器（HSBBR）处理高盐废水，实现了同步短程硝化反硝化脱氮。考察了运行模式对同步短程硝化反硝化系统COD去除和脱氮性能的影响，利用高通量测序技术分析了微生物群落的变化。结果表明：当反应器以缺氧/好氧交替模式运行时（缺氧/好氧时间比为2.0 h/4.5 h），NH₃-N、总无机氮（TIN）和COD去除率分别为95.00%、84.83%和86.72%，出水中含有NO₂^--N和NO₃^--N。以完全好氧模式运行时（缺氧/好氧时间比为0.0/6.5 h），NH₃-N去除率达到100.00%，TIN和COD去除率分别为85.94%和89.46%，出水中只含有NO₂^--N。高通量测序结果表明：在门水平上，2种模式下生物膜和悬浮污泥中的优势菌均为变形菌门（Proteobacteria）和拟杆菌门（Bacteroidetes）；Nitrosomonas是本研究检测出的唯一氨氧化菌（AOB）属，当反应器由缺氧/好氧交替模式转换为完全好氧模式时，悬浮污泥和生物膜中Nitrosomonas的相对丰度均增加；悬浮污泥和生物膜中优势反硝化菌属相似，包括Candidatus_Competibacter、Paracoccus、Thauera和Denitratisoma，在完全好氧模式下，悬浮污泥和生物膜中Candidatus_Competibacter和Thauera的相对丰度较低，而Paracoccus和Denitratisoma的相对丰度较高。多种反硝化菌与氨氧化菌的共同作用，使反应器能够实现高效同步短程硝化反硝化脱氮。
- 高盐 /
- HSBBR /
- 运行模式 /
- 同步短程硝化反硝化 /
- 微生物群落特征
Abstract: In this study, simultaneous partial nitrification and denitrification (SPND) process was successfully established for the treatment of high-salinity wastewater in a hybrid sequencing batch biofilm reactor (HSBBR). The effect of operational mode on the reactor performance and microbial community was investigated. When the reactor was operated in alternating anoxic/aerobic mode (anoxic/aerobic hour ratio was 2.0 h/4.5 h), the removal efficiencies of NH₄⁺-N, total inorganic nitrogen (TIN) and COD were 95.00%, 84.83% and 86.72%, respectively, and the effluent contained nitrite and nitrate. When operational mode was switched to fully aerobic mode (anoxic/aerobic hour ratio was 0.0/6.5 h), removal efficiencies of NH₃-N, TIN and COD increased to 100.00%, 85.94% and 89.46%, and nitrate was the only nitrogen component in the effluent. The high-throughput sequencing results showed that the dominant phyla were Proteobacteria and Bacteroidetes in both suspended sludge (S-sludge) and biofilm in two modes. Genus Nitrosomonas was the only ammonia-oxidizing bacteria (AOB) detected in this study and appeared higher relative abundance in S-sludge and biofilm samples in the fully aerobic mode compared to the alternating anoxic/aerobic mode. S-sludge and biofilm shared similar dominant denitrifying bacteria (DNB), including genera Candidatus_Competibacter, Paracoccus, Thauera and Denittrasoma. The relative abundance of Candidatus_Competibacter and Thauera decreased in fully aerobic mode, while Paracoccus and Denitratisoma showed increasing tendency. Multiple DNB genera accompanied by AOB contributed to the efficient nitrogen removal via SPND process.
- high salinity /
- hybrid sequencing batch biofilm reactor (HSBBR) /
- operation mode /
- simultaneous partial nitrification and denitrification /
- microbial community

HTML全文

参考文献(39)

FERRER-POLONIO E, GARCÍA-QUIJANO N T, MENDOZA-ROCA J A, et al. Effect of alternating anaerobic and aerobic phases on the performance of a SBR treating effluents with high salinity and phenols concentration[J]. Biochemical Engineering Journal, 2016,113:57-65.

LIAO R H, LI Y, WANG Z, et al. 454 pyrosequencing analysis on microbial diversity of an expanded granular sludge bed reactor treating high NaCl and nitrate concentration wastewater[J]. Biotechnology and Bioprocess Engineering, 2014,19(1):183-190.

DAN N P, VISVANATHAN C, BASU BISWADEEP. Comparative evaluation of yeast and bacterial treatment of high salinity wastewater based on biokinetic coefficients[J]. Bioresource Technology, 2003,87:51-56.

WANG J L, GONG B Z, HUANG W, et al. Bacterial community structure in simultaneous nitrification, denitrification and organic matter removal process treating saline mustard tuber wastewater as revealed by 16S rRNA sequencing[J]. Bioresource Technology, 2017,228:31-38.

SHE Z L, ZHAO L T, ZHANG X L,et al. Partial nitrification and denitrification in a sequencing batch reactor treating high-salinity wastewater[J]. Chemical Engineering Journal, 2016, 288:207-215.

YANG S, YANG F L. Nitrogen removal via short-cut simultaneous nitrification and denitrification in an intermittently aerated moving bed membrane bioreactor[J]. Journal of Hazardous Materials, 2011,195:318-323.

ZHANG L Q, WEI C H, ZHANG K F, et al. Effect of temperature on simultaneous nitrification and denitrification by using sequencing batch reactor[J]. Bioprocess and Biosystems Engineering, 2009, 32(2):175-182.

于德爽,袁梦飞,王晓霞,等.厌氧/好氧SPNDPR系统实现低C/N城市污水同步脱氮除磷的优化运行[J]. 环境科学,2018, 39(11):5066-5073.

孙赛武,杨朝晖,曾光明,等. 进水模式对SBR性能及氮形态转化的影响[J]. 环境科学,2009, 30(1):121-126.

曾薇,彭永臻,王淑莹. SBR工艺交替硝化反硝化运行方式的可行性研究[J]. 环境科学学报,2004, 24(4):576-580.

LI H S, ZHOU S Q, HUANG G T, et al. Partial nitritation of landfill leachate with varying influent composition under intermittent aeration conditions[J]. Process Safety and Environmental Protection, 2013, 91(4):285-294.

ZHANG F, LI P, CHEN M S, et al. Effect of operational modes on nitrogen removal and nitrous oxide emission in the process of simultaneous nitrification and denitrification[J]. Chemical Engineering Journal, 2015, 280:549-557.

王芳,葛桂波. SBR不同运行模式对好氧颗粒污泥性能的影响[J]. 苏州科技学院学报(工程技术版),2015, 28(4):18-23.

高春娣,王惟肖,李浩,等. SBR法交替缺氧好氧模式下短程硝化效率的优化[J]. 中国环境科学,2015, 35(2):403-409.

国家环境保护总局, 水和废水监测分析方法编委会.水和废水监测分析方法[M]. 4版. 北京:中国环境出版社,2002.

王晓霞,王淑莹,赵冀,等.厌氧/好氧SNEDPR系统处理低C/N污水的优化运行[J]. 中国环境科学,2016,36(9):2672-2680.

HE S L, NIU Q G, LI Y Y, et al. Factors associated with the diversification of the microbial communities within different natural and artificial saline environments[J]. Ecological Engineering,2015, 83:476-484.

黄郑郑,曹刚,李紫惠,等. XH02菌强化反应器脱氮过程中菌群结构的高通量分析[J]. 中国环境科学,2107,37(5):1922-1929.

WANG Y Y, CHEN J, ZHOU S, et al. 16S rRNA gene high-throughput sequencing reveals shift in nitrogen conversion Related microorganisms in a CANON system in response to salt stress[J]. Chemical Engineering Journal, 2017,317:512-521.

侯爱月,李军,卞伟,等. 不同短程硝化系统中微生物群落结构的对比分析[J]. 中国环境科学,2106,36(2):428-436.

HU M, WANG X H, WEN X H, et al. Microbial community structures in different wastewater treatment plants as revealed by 454-pyrosequencing analysis[J]. Bioresource Technology, 2012,117:72-79.

SUTO R, ISHIMOTO C, CHIKYU M, et al. Anammox biofilm in activated sludge swine wastewater treatment plants[J]. Chemosphere, 2017, 167:300-307.

CHEN J F, YANG Y W, LIU Y Y, et al. Bacterial community shift in response to a deep municipal tail wastewater treatment system[J]. Bioresource Technology, 2019, 281:195-201.

HUANG W Y, SHE Z L, GAO M C, et al. Effect of anaerobic/aerobic duration on nitrogen removal and microbial community in a simultaneous partial nitrification and denitrification system under low salinity[J]. Science of the Total Environment, 2019, 651:859-870.

ZHANG L, SUA F, WANG N, et al. Biodegradability enhancement of hydrolyzed polyacrylamide wastewater by a combined Fenton-SBR treatment process[J]. Bioresource Technology, 2019, 27:99-107.

邢金良,张岩,陈昌明,等. CEM-UF组合膜-硝化/反硝化系统处理低C/N废水及种群结构分析[J]. 环境科学,2018,39(3):1342-1349.

TANG B, CHEN Q Y, BIN L Y, et al. Insight into the microbial community and its succession of a coupling anaerobic-aerobic biofilm on semi-suspended bio-carriers[J]. Bioresource Technology, 2018, 247:591-598.

TAYLAR A E, BOTTOMLEY P J. Nitrite production by Nitrosomonas europaea and Nitrosospira sp.AV in soils at different solution concentrations of ammonium[J]. Soil Biology and Biochemistry, 2006, 38(4):828-836.

HEYLEN K, GEVERS D, VANPARYS B, et al. The incidence of nirS and nirK and their genetic heterogeneity in cultivated denitrifiers[J]. Environmental microbiology,2006, 8(11):2012-2021.

RUBIO-RINCÓN F J, LOPEZ-VAZQUEZ C M, WELLES L, et al. Cooperation between Candidatus Competibacter and Candidatus Accumulibacter clade I, in denitrification and phosphate removal processes[J]. Water Research, 2017, 120:156-164.

TIAN Q, ZHUANG L J, ONG S K, et al. Phosphorus (P) recovery coupled with increasing influent ammonium facilitated intracellular carbon source storage and simultaneous aerobic phosphorus & nitrogen removal[J]. Water Research, 2017,119:267-275.

HOSSAIN M I, CHENG L, CORD-RUWISCH R. Energy efficient COD and N-removal from high-strength wastewater by a passively aerated GAO dominated[J]. Bioresource Technology, 2019, 283:148-158.

ZENG R J, YUAN Z G, KELLER J. Enrichment of denitrifying glycogen-accumulating on ganisms in anaerobic/anoxic activated sludge system[J]. Biotechnology and Bioengineering, 2003, 81(4):397-404.

HOSSAIN M I, PAPARINI A, CORD-RUWISCH R. Rapid adaptation of activated sludge bacteria into a glycogen accumulating biofilm enabling anaerobic BOD uptake[J]. Bioresource Technology,2017, 228:1-8.

SHI Z, ZHANG Y, ZHOU J T, et al. Biological removal of nitrate and ammonium under aerobic atmosphere by Paracoccus versutus LYM[J]. Bioresource Technology, 2013,148:144-148.

MEDHI K, THAKUR I S. Bioremoval of nutrients from wastewater by a denitrifier Paracoccus denitrificans ISTOD1[J]. Bioresource Technology Reports, 2018,1:56-60.

ZHANG H H, ZHAO Z F, CHEN S N, et al. Paracoccus versutus KS293 adaptation to aerobic and anaerobic denitrification:insights from nitrogen removal functional gene abundance and proteomic profiling analysis[J]. Bioresource Technology, 2018,260:321-328.

YAN X M, ZHU C Y, HUANG B, et al. Enhanced nitrogen removal from electroplating tail wastewater through two-staged anoxic-oxic (A/O) process[J]. Bioresource Technology, 2018, 247:157-164.

WU D, YI X Y, TANG R, et al. Single microbial fuel cell reactor for coking wastewater treatment:simultaneous carbon and nitrogen removal with zero alkaline consumption[J]. Science of the Total Environment, 2018, 621:497-506.

施引文献

资源附件(0)

访问统计