异养硝化-好氧反硝化菌株GNR选育及对猪场废水脱氮实验

谭俊杰; 李立君; 李雪洁; 韩天放; 胡筱敏; 赵鑫

doi:10.13205/j.hjgc.202102004

异养硝化-好氧反硝化菌株GNR选育及对猪场废水脱氮实验

doi: 10.13205/j.hjgc.202102004

1. 东北大学资源与土木工程学院, 沈阳 110819;
2. 东京工业大学环境社会理工学院, 东京152-8550;
3. 盘锦市生态环境局, 辽宁盘锦 124010

基金项目:

水体污染控制与治理科技重大专项（2013ZX07202-010）；中央直属高校基本科研业务费项目（N170104021，N182508026）。

详细信息

作者简介:
谭俊杰(1997-),女,硕士研究生,主要研究方向为水污染控制与化工分离合成。junjietan0308@gmail.com

通讯作者:
赵鑫(1982-),男,博士,副教授,主要研究方向为水污染控制与微生物分子生态学。zhaoxin@mail.neu.edu.cn

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出版历程
- 收稿日期: 2019-10-28
- 网络出版日期: 2021-07-19

ISOLATION OF A HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFYING STRAIN GNR AND ITS APPLICATION OF AMMONIA REMOVAL OF SWINE WASTEWATER

1. Department of Environmental Engineering, School of Resources and Civil Engineering, Northeastern University, Shenyang 110819, China;
2. School of Environment and Society, Tokyo Institute of Technology, Tokyo 152-8550, Japan;
3. Bureau of Ecology and Environment of Panjin, Panjin 124010, China

摘要

摘要: 从养猪场废水中分离获得1株异养硝化-好氧反硝化菌，编号为GNR，经形态学及系统发育鉴定，确定为不动杆菌属（Acinetobacter）的新菌株。通过条件优化实验获得菌株GNR的异养硝化和好氧反硝化的最佳碳源均为柠檬酸三钠，最佳C/N均为10：1，最佳初始pH分别为8.0和6.0。在最优条件下，初始NH₄⁺-N和NO₃^--N浓度均为50.0 mg/L，30 ℃、125 r/min培养72 h后，硝化和反硝化效率均达到99.0%。菌株GNR在最适宜条件下，对稀释后的实际养猪场废水的COD和NH₄⁺-N去除量最高可达129，73 mg/（L·g），具有良好的污水脱氮潜在应用潜力。
- 生物脱氮 /
- 异养硝化 /
- 好氧反硝化 /
- Acinetobacter /
- 养猪场废水
Abstract: In this study, a heterotrophic nitrification-aerobic denitrifying strain named GNR was isolated from swine wastewater. It was identified as a new strain of genus Acinetobacter through morphological and phylogenetic analysis. The optimal heterotrophic nitrification and aerobic denitrification conditions were investigated as the carbon source of trisodium citrate, C/N ratio of 10:1, initial pH of 8.0 and 6.0, respectively. The maximum removal efficiencies of ammonia and nitrate nitrogen with initial concentration of 50.0 mg/L both reached over 99.0% by strain GNR, after 30℃, 125 r/min shaking cultivation for 72 h. Under the optimal condition, the COD and ammonia removal efficiencies in diluted swine wastewater treatment reached 129, 73 mg/(L·g), respectively. The experimental results indicated that strain GNR had good application potential in wastewater denitrification.
- biological nitrogen removal /
- heterotrophic nitrification /
- aerobic denitrification /
- Acinetobacter /
- swine wastewater

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参考文献(20)

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