ISOLATION OF A HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFYING STRAIN GNR AND ITS APPLICATION OF AMMONIA REMOVAL OF SWINE WASTEWATER
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摘要: 从养猪场废水中分离获得1株异养硝化-好氧反硝化菌,编号为GNR,经形态学及系统发育鉴定,确定为不动杆菌属(Acinetobacter)的新菌株。通过条件优化实验获得菌株GNR的异养硝化和好氧反硝化的最佳碳源均为柠檬酸三钠,最佳C/N均为10:1,最佳初始pH分别为8.0和6.0。在最优条件下,初始NH4+-N和NO3--N浓度均为50.0 mg/L,30 ℃、125 r/min培养72 h后,硝化和反硝化效率均达到99.0%。菌株GNR在最适宜条件下,对稀释后的实际养猪场废水的COD和NH4+-N去除量最高可达129,73 mg/(L·g),具有良好的污水脱氮潜在应用潜力。
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关键词:
- 生物脱氮 /
- 异养硝化 /
- 好氧反硝化 /
- Acinetobacter /
- 养猪场废水
Abstract: In this study, a heterotrophic nitrification-aerobic denitrifying strain named GNR was isolated from swine wastewater. It was identified as a new strain of genus Acinetobacter through morphological and phylogenetic analysis. The optimal heterotrophic nitrification and aerobic denitrification conditions were investigated as the carbon source of trisodium citrate, C/N ratio of 10:1, initial pH of 8.0 and 6.0, respectively. The maximum removal efficiencies of ammonia and nitrate nitrogen with initial concentration of 50.0 mg/L both reached over 99.0% by strain GNR, after 30℃, 125 r/min shaking cultivation for 72 h. Under the optimal condition, the COD and ammonia removal efficiencies in diluted swine wastewater treatment reached 129, 73 mg/(L·g), respectively. The experimental results indicated that strain GNR had good application potential in wastewater denitrification. -
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