HETEROTROPHIC NITRIFICATION-AEROBIC DENITRIFICATION CHARACTERISTICS OF ALCALIGENES FAECALIS WT14 AND ITS POTENTIAL APPLICATION IN AMMONIA-RICH WASTEWATER
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摘要: 异养硝化-好氧反硝化(HN-AD)菌具有同步硝化反硝化的特性,在污水生物处理方面引起了广泛关注。为探究HN-AD菌在处理高氨废水方面的应用潜力,以前期从人工湿地底泥基质中分离出的HN-AD菌Alcaligenes faecalis WT14为研究对象,在好氧条件下研究了该菌株的脱氮特性,并对其高氨废水去除潜力和环境因子交互作用对其脱氮效率影响方面进行了评估。实验结果表明,WT14表现出高效的HN-AD性能,能够同步脱氮除碳,在好氧条件下能够去除98.9%的NO3--N和92.7%的TOC,且不含NO2--N的积累。同时,在游离氨(FA)浓度为1084.6 mg/L的高NH4+-N(2000 mg/L)废水中,NH4+-N去除率约为43%,脱氮速率可达55.9 mg/(L·h),且无NO3--N的积累。响应面结果表明:其最佳脱氮条件为20.6 ℃, 113.5 r/min, C/N=10.8,pH=8.4。总体而言,菌株WT14具有高效处理高氨废水的应用潜力。
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关键词:
- 异养硝化-好氧反硝化 /
- 同步脱氮 /
- Alcaligenes faecalis /
- 游离氨 /
- 氨氮
Abstract: The ability of HN-AD bacteria to simultaneous nitrification and denitrification caused widespread concern in biological wastewater treatment. In order to investigate the application potential of HN-AD bacteria in ammonia-rich wastewater, the HN-AD bacteria Alcaligenes faecalis WT14 was isolated from the substrate of constructed wetland sediment. The nitrogen removal characteristics of this strain were studied under aerobic conditions, and the effect of the interaction between the removal potential of high ammonia wastewater and environmental factors on its nitrogen removal efficiency was evaluated. The strain WT14 exhibited excellent HN-AD performance and high ammonia tolerance. It could remove 98.9% of NO3--N and 92.7% of TOC under aerobic conditions, and no NO2--N accumulation took place in the whole course. At the same time, the strain WT14 treated high ammonia (2000 mg/L) wastewater with a free ammonia concentration of 1084.6 mg/L and removed about 43% NH4+-N without accumulation of NO3--N. The results of response surface methodology showed that the optimal conditions for denitrification were as follows:20.6℃, 113.5 r/min, C/N of 10.8, and pH of 8.4. Overall, the strain WT14 has good potential for ammonia-rich wastewater treatment. -
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