微藻混合培养强化餐厨沼液处理效果及机制

刘艳; 杨敏; 陈宏; 蒋朝晖; 赵文玉; 王泓; 张俊亚

doi:10.13205/j.hjgc.202103008

微藻混合培养强化餐厨沼液处理效果及机制

doi: 10.13205/j.hjgc.202103008

刘艳^1,2,
杨敏^1,2,3, ,,
陈宏²,
蒋朝晖^1,2,
赵文玉^1,2,
王泓²,
张俊亚³

1. 长沙理工大学化学与食品工程学院, 长沙 410114;
2. 长沙理工大学洞庭湖水环境治理与生态修复湖南省重点实验室, 长沙 410114;
3. 中国科学院生态环境研究中心, 北京 100085

基金项目:

湖南省自然科学基金（2019JJ50646）；湖南省科技创新项目重点研发计划（2018SK2011）；湖南省教育厅基金一般项目（18C0206）；洞庭湖水环境治理与生态修复湖南省重点实验室开放基金（2018DT02）。

详细信息

作者简介:
刘艳(1995-),女,硕士,主要研究方向为微藻与污水处理。2487505289@qq.com

通讯作者:
杨敏(1988-),男,博士、讲师,主要研究方向为污水处理与资源化。min_yang@csust.edu.cn

计量
- 文章访问数: 266
- HTML全文浏览量: 32
- PDF下载量: 12
- 被引次数: 0
出版历程
- 收稿日期: 2020-03-08
- 网络出版日期: 2021-07-19

ENHANCEMENT AND MECHANISM OF MIXED ALGAE CULTIVATION FOR TREATMENT OF KITCHEN WASTE DIGESTATE EFFLUENT

LIU Yan^1,2,
YANG Min^{1,2,3
, ,},
CHEN Hong²,
JIANG Zhao-hui^1,2,
ZHAO Wen-yu^1,2,
WANG Hong²,
ZHANG Jun-ya³

1. School of Chemical and Food Engineering, Changsha University of Science and Technology, Hunan, Changsha 410114, China;
2. Key Laboratory of Dongting Lake Aquatic Eco-Environmental Control and Restoration of Hunan Province, Changsha University of Science and Technology, Changsha 410114, China;
3. Chinese Academy of Sciences, Center for Eco-Environmental Research, Beijing 100085, China

摘要

摘要: 利用微藻混合培养处理餐厨垃圾消化沼液具有高效固碳脱氮的优势，然而存在优选混合培养比和协同强化作用机制不明的问题。对比分析了普通小球藻（Chlorella vulgaris）、斜生栅藻（Scenedesmus obliquus）和雨生红球藻（Haematococcus pluvislis）在单一和混合培养模式下的微藻生长特性和沼液处理效果，研究了微藻的胞外可溶性聚合物（SAP）对微藻生长的促进效果及竞争协同机制。结果表明：斜生栅藻和雨生红球藻为最佳的微藻组合，最大生物量为0.655 g/L，COD去除率为76.2%，NH₄⁺-N去除率为60.1%；雨生红球藻和斜生栅藻之间存在hormesis效应，释放的可溶性微藻产物SAP作为异种化感物可被对方利用，且两者对不同污染组分同化能力存在差异性，形成协同竞争作用缓解了高浓度废水及SAP对微藻生长的抑制，可为微藻混合培养提高生物质产量与强化沼液处理效果提供参考。
- 微藻 /
- 餐厨垃圾消化沼液 /
- 混合培养 /
- 胞外可溶性聚合物
Abstract: The use of microalgae mixed culture for the treatment of kitchen wastes digestate effluent has the advantages of efficient carbon fixation and denitrification. However, the preferred mixed culture ratio and synergistic enhancement mechanism are still unclear. In this study, the growth characteristics of microalgae and efficiency of digestate effluent treatment of Chlorella vulgaris, Scenedesmus obliquus, and Haematococcus pluvislis in single and mixed culture modes were compared. The stimulation effect of the soluble algae products (SAP) on microalgae growth and the synergistic mechanism of competition between different microalgae were studied. The mixture cultivation of Scenedesmus obliquus and Haematococcus pluvialis was the optimized match with maximum biomass of 0.655 g/L, COD removal rate of 76.2% and NH₄⁺-N removal rate of 60.1%. Interspecific SAPs produced by Haematococcus pluvialis and Scenedesmus obliquus could be consumed as an allelo-chemicals by each other, following by the hormesis effect, and the two microalgae had different assimilation abilities onto different pollutants, forming a synergistic competition to alleviate the inhibition of high concentration wastewater and SAP generated by themselves. This mechanism of synergistic competition could explain the reason why mixed culture of microalgae increased biomass yield and enhanced the treatment of kitchen waste digestate effluent.
- microalgae /
- kitchen waste digestate effluent /
- mixed culture /
- soluble algae product

HTML全文

参考文献(31)

[1]	ZHANG C S, SU H J, BAEYENS J, et al. Reviewing the anaerobic digestion of food waste for biogas production[J]. Renewable and Sustainable Energy Reviews, 2014, 38:383-392.
[2]	OSWALD W J, GOTAAS H B, GOLUEKE C G, et al. Algae in waste treatment[J]. Sewage and Industrial Wastes, 1957, 29(4):437-455.
[3]	KHAN A, SHAHID A, CHENG H, et al. Omics technologies for microalgae-based fuels and chemicals; challenges and opportunities[J]. Protein and Peptide Letters, 2018, 25(2):99-107.
[4]	CHENG J, YE Q, XU J, et al. Improving pollutants removal by microalgae Chlorella PY-ZU1 with 15% CO₂ from undiluted anaerobic digestion effluent of food wastes with ozonation pretreatment[J]. Bioresource Technology, 2016, 216:273-279.
[5]	BIDDANDA B, BENNER R. Carbon nitrogen and carbohydrate fluxes during the production of particulate and dissolved organic matter by marine phytoplankton[J]. Limnology and Oceanography, 1997, 42:506-518.
[6]	LÓPEZ-SERNA R, GARCÍA D, BOLADO S, et al. Photobioreactors based on microalgae-bacteria and purple phototrophic bacteria consortia:a promising technology to reduce the load of veterinary drugs from piggery wastewater[J]. Science of the Total Environment,2019, 692:259-266.
[7]	PRATT R. Studies on Chlorella vulgaris.V. some properties of the growth-inhibitor formed by Chlorella cells[J]. American Journal of Botany, 1942, 29(2):142-148.
[8]	冯思然,朱顺妮,王忠铭. 微藻污水处理研究进展[J].环境工程, 2019,37(4):57-62.
[9]	SHAHID A,MALIK S,ZHU H, et al. Cultivating microalgae in wastewater for biomass production, pollutant removal, and atmospheric carbon mitigation:a review[J]. The Science of the Total Environment, 2020,704:135303.
[10]	CHINNASAMY S, BHATNAGAR A, HUNT R W, et al. Microalgae cultivation in a wastewater dominated by carpet mill effluents for biofuel applications[J]. Bioresource Technology, 2010, 101:3097-3105.
[11]	HUY M, KUMAR G, KIM H W, et al. Photoautotrophic cultivation of mixed microalgae consortia using various organic waste streams towards remediation and resource recovery[J]. Bioresource Technology, 2018, 247:576-581.
[12]	MICHELON W, DA SILVA M L B, MEZZARI M P, et al. Effects of nitrogen and phosphorus on biochemical composition of microalgae polyculture harvested from phycoremediation of piggery wastewater digestate[J]. Applied Biochemistry and Biotechnology, 2016, 178:1407-1419.
[13]	CARDINALE B J. Biodiversity improves water quality through niche partitioning[J]. Nature, 2012, 472:86-89.
[14]	PHATARPEKAR P V, SREEPADA R A, PEDNEKAR C, et al. A comparative study on growth performance and biochemical composition of mixed culture of Isochrysis galbana and Chaetoceros calcitrans with monocultures[J]. Aquaculture, 2000, 181(1):141-155.
[15]	UCHIDA T, TODA S, MATSUYAMA Y, et al. Interactions between the red tide dinoflagellates Heterocapsa circularisquama and Gymnodinium mikimotoi in laboratory culture[J]. Journal of Experimental Marine Biology and Ecology, 1999, 241(2):285-299.
[16]	范婧,周北海,张鸿涛,等. 再生水补充经管水体中藻类的生长比较[J].环境科学研究, 2012,25(5):573-577.
[17]	QIAN Y P,LI X T,TIAN R N. Effects of aqueous extracts from the rhizome of Pontederia cordata on the growth and interspecific competition of two algal species[J]. Ecotoxicology and Environmental Safety, 2019, 168:401-407.
[18]	ZHANG T Y, YU Y, WU Y H, et al. Inhibitory effects of soluble algae products (SAP) released by Scenedesmus sp LX1 on its growth and lipid production[J]. Bioresource Technology, 2013, 146:643-648.
[19]	WANG M, YANG H, ERGAS S J, et al. A novel shortcut nitrogen removal process using an algal-bacterial consortium in a photo-sequencing batch reactor (PSBR)[J]. Water Research, 2015, 87:38-48.
[20]	YANG S, XU J, WANG Z M, et al. Cultivation of oleaginous microalgae for removal of nutrients and heavy metals from biogas digestates[J]. Journal of Cleaner Production, 2017, 164:793-803.
[21]	刘祥,王婧瑶,吴娟娟,等. 微藻固定化条件优化及其污水氨氮去除潜力分析[J]. 环境科学,2019, 40(7):3126-3134.
[22]	罗智展,舒琥,许瑾,等. 利用微藻处理污水的研究进展[J]. 水处理技术, 2019, 45(10):17-39.
[23]	TORU Y,NAOYA S,KAZUTOSHI I, et al. Polyethyleneimine-induced astaxanthin accumulation in the green alga Haematococcus pluvialis by increased oxidative stress[J]. Journal of Bioscience and Bioengineering, 2019, 128(6):751-754.
[24]	WHITTON R, MÉVEL A L, PIDOU M, et al. Influence of microalgal N and P composition on wastewater nutrient remediation[J]. Water Research, 2016, 91:371-378.
[25]	ZHOU W G, MIN M, LI Y C, et al. A hetero-photoautotrophic two-stage cultivation process to improve wastewater nutrient removal and enhance algal lipid accumulation[J]. Bioresource Technology, 2012, 110:448-455.
[26]	SINGH G, THOMAS P B. Nutrient removal from membrane bioreactor permeate using microalgae and in a microalgae membrane photoreactor[J]. Bioresource Technology, 2012, 117:80-85.
[27]	WÁGNER D S, VALVERDE-PÉREZ B, SAEBØ M, et al. Towards a consensus-based biokinetic model for green microalgae-The ASM-A[J]. Water Research, 2016, 103:485-499.
[28]	WU Y H, ZHU S F, YU Y, et al. Mixed cultivation as an effective approach to enhance microalgal biomass and triacylglycerol production in domestic secondary effluent[J]. Chemical Engineering Journal, 2017, 328:665-672.
[29]	MOHAMMAD R,QU M X,MD A A, et al. Investigating the potentiality of Scenedesmus obliquus and Acinetobacter pittii partnership system and their effects on nutrients removal from synthetic domestic wastewater[J]. Bioresource Technology, 2020, 299:122571.
[30]	ANSARI F A,RAVINDRAN B,GUPTA S K, et al. Techno-economic estimation of wastewater phycoremediation and environmental benefits using Scenedesmus obliquus microalgae[J]. Journal of Environmental Management, 2019, 240:293-302.
[31]	刘峰. 雨生红球藻优良藻株的诱变选育及其培养基的氮磷浓度的优化[D].青岛:中国海洋大学,2015.