ANALYSIS ON CHANGE OF MICROBIAL COMMUNITY IN AAO BIOCHEMICAL SYSTEM OF A SEWAGE TREATMENT PLANT UNDER HIGH SALINITY ENVIRONMENT
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摘要: 为研究高盐度环境下AAO生化系统的活性污泥优势微生物,利用高通量测序技术对不同时期污泥进行微生物结构进行分析。结果表明:ρ(Cl-)从1000 mg/L上升到5000 mg/L的过程中,微生物群落结构发生明显变化。持久型OTUs占总序列90.59%,其中Proteobacteria(变形菌门)相对丰度始终在40%以上,Chiorobi(绿菌门)相对丰度从6.11%上升至16.13%。微生物属水平分析发现,Methyloceanibacter(16.94%~27.44%)是高盐条件下主要的有机物去除菌属,Ignavibacterium(18.43%~26.78%)是主要除硫菌属,Dechloromonas(1.52%~3.05%)、Nitrospirae(1.9%~8.84%)、Nitrosomonas(1%左右)是主要的脱氮菌属。Abstract: In order to study the dominant microorganisms of activated sludge in AAO biochemical system at high Cl- concentration, high-throughput sequencing technology was used to analyze the microbial structure of sludge in different periods. The results showed that the microbial community structure changed obviously when the Cl- concentration increased from 1000 mg/L to 5000 mg/L. Persistent OTUs accounted for 90.59% of the total sequence. The relative abundance of Proteobacteria in the bacterial community was stable at 40% above. The relative abundance of Chiorobi increased from 6.11% to 16.13%. Generic level analysis of microorganisms showed that Methyloceanibacter (16.94%~27.44%) was the main organic matter removal bacteria under the condition of high salinity. Ignavibacterium (18.43%~26.78%) played an important role in the removal of sulfides. Dechloromonas (1.52%~3.05%), Nitrospirae (1.9%~8.84%) and Nitrosomonas (about 1%) was the main bacteria to remove nitrogen pollutants.
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