ANALYSIS ON CHANGE OF MICROBIAL COMMUNITY IN AAO BIOCHEMICAL SYSTEM OF A SEWAGE TREATMENT PLANT UNDER HIGH SALINITY ENVIRONMENT
-
摘要: 为研究高盐度环境下AAO生化系统的活性污泥优势微生物,利用高通量测序技术对不同时期污泥进行微生物结构进行分析。结果表明:ρ(Cl-)从1000 mg/L上升到5000 mg/L的过程中,微生物群落结构发生明显变化。持久型OTUs占总序列90.59%,其中Proteobacteria(变形菌门)相对丰度始终在40%以上,Chiorobi(绿菌门)相对丰度从6.11%上升至16.13%。微生物属水平分析发现,Methyloceanibacter(16.94%~27.44%)是高盐条件下主要的有机物去除菌属,Ignavibacterium(18.43%~26.78%)是主要除硫菌属,Dechloromonas(1.52%~3.05%)、Nitrospirae(1.9%~8.84%)、Nitrosomonas(1%左右)是主要的脱氮菌属。Abstract: In order to study the dominant microorganisms of activated sludge in AAO biochemical system at high Cl- concentration, high-throughput sequencing technology was used to analyze the microbial structure of sludge in different periods. The results showed that the microbial community structure changed obviously when the Cl- concentration increased from 1000 mg/L to 5000 mg/L. Persistent OTUs accounted for 90.59% of the total sequence. The relative abundance of Proteobacteria in the bacterial community was stable at 40% above. The relative abundance of Chiorobi increased from 6.11% to 16.13%. Generic level analysis of microorganisms showed that Methyloceanibacter (16.94%~27.44%) was the main organic matter removal bacteria under the condition of high salinity. Ignavibacterium (18.43%~26.78%) played an important role in the removal of sulfides. Dechloromonas (1.52%~3.05%), Nitrospirae (1.9%~8.84%) and Nitrosomonas (about 1%) was the main bacteria to remove nitrogen pollutants.
-
[1] WEN Y, JIN Y X, WANG J Y, et al. MiSeq sequencing analysis of bacterial community structures in wastewater treatment plants[J]. Polish Journal of Environmental Studies, 2015,24(4):1809-1815. [2] FAN X Y, GAO J F, PAN K L, et al. Temporal dynamics of bacterial communities and predicted nitrogen metabolism genes in a full-scale wastewater treatment plant[J]. RSC Advances, 2017,7(89):56317-56327. [3] SHU D T, HE Y L, YUE H, et al. Metagenomic and quantitative insights into microbial communities and functional genes of nitrogen and iron cycling in twelve wastewater treatment systems[J]. Chemical Engineering Journal, 2016,290:21-30. [4] JU F, LI B, MA L P, et al. Antibiotic resistance genes and human bacterial pathogens:co-occurrence, removal, and enrichment in municipal sewage sludge digesters[J]. Water Research, 2016,91:1-10. [5] 高晨晨, 郑兴灿, 游佳, 等. 城市污水脱氮除磷系统的活性污泥菌群结构特征[J].中国给水排水, 2015,31(23):37-42. [6] CHEN Y, LAN S, WANG L, et al. A review:driving factors and regulation strategies of microbial community structure and dynamics in wastewater treatment systems[J]. Chemosphere, 2017,174:173-182. [7] HONG J M, LI W B, LIN B, et al. Deciphering the effect of salinity on the performance of submerged membrane bioreactor for aquaculture of bacterial community[J]. Desalination, 2013,316:23-30. [8] WANG Z C, GAO M C, SHE Z L, et al. Effects of salinity on performance, extracellular polymeric substances and microbial community of an aerobic granular sequencing batch reactor[J]. Separation and Purification Technology, 2015,144:223-231. [9] WILSON L P, HL L, ES S, et al. Microbial community acclimation enhances waste hydrolysis rates under elevated ammonia and salinity conditions[J]. Bioresour Technol, 2013,146:15-22. [10] KULKARNI P. Nitrophenol removal by simultaneous nitrification denitrification (SND) using T. pantotropha in sequencing batch reactors (SBR)[J]. Bioresource Technology, 2013,128:273-280. [11] FIGUEROA M, MOSQUERA-CORRAL A, CAMPOS J L, et al. Treatment of saline wastewater in SBR aerobic granular reactors[J]. Water Science and Technology, 2008,58(2):479-485. [12] BASSIN J P, KLEEREBEZEM R, MUYZER G, et al. Effect of different salt adaptation strategies on the microbial diversity, activity, and settling of nitrifying sludge in sequencing batch reactors[J]. Applied Microbiology Biotechnology, 2012,93(3):1281-1294. [13] XIA Y, WEN X H, ZHANG B, et al. Diversity and assembly patterns of activated sludge microbial communities:a review[J]. Biotechnology Advances, 2018,36(4):1038-1047. [14] KLINDWORTH A, PRUESSE E, SCHWEER T, et al. Evaluation of general 16S ribosomal RNA gene PCR primers for classical and next-generation sequencing-based diversity studies[J]. Nucleic Acids Research, 2013,41(1):e1. [15] SCHLOSS P D, WESTCOTT S L, RYABIN T, et al. Introducing mothur:open-source, platform-independent, community-supported software for describing and comparing microbial communities[J]. Applied Environmental Microbiology, 2009,75(23):7537-7541. [16] DESANTIS T Z, HUGENHOLTZ P, LARSEN N, et al. Greengenes, a chimera-checked 16S rRNA gene database and workbench compatible with ARB[J]. Applied Environmental Microbiology, 2006,72(7):5069-5072. [17] 樊晓燕, 高景峰, 王时杰, 等. 同步脱氮除磷系统中两种颜色好氧颗粒污泥的微生物群落特征[J].环境科学研究, 2017,30(8):1287-1294. [18] CALDERÓN K, SPOR A, BREUIL M C, et al. Effectiveness of ecological rescue for altered soil microbial communities and functions[J]. The ISME Journal, 2016,11(1):272-283. [19] MAR J S, LAMERE B J, LIN D L, et al. Disease severity and immune activity relate to distinct interkingdom gut microbiome states in ethnically distinct ulcerative colitis patients[J]. mBio, 2016,7(4):e01072-16. [20] MITTER E K, DE FREITAS J R, GERMIDA J J. Bacterial root microbiome of plants growing in oil sands reclamation covers[J]. Frontiers in Microbiology, 2017,8:849. [21] VAN DER GAST C J, WALKER A W, STRESSMANN F A, et al. Partitioning core and satellite taxa from within cystic fibrosis lung bacterial communities[J]. The ISME Journal, 2011,5(5):780-791. [22] ZHANG T, SHAO M F, YE L. 454 pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants[J]. The ISME Journal, 2012,6(6):1137-1147. [23] YOON D N, PARK S J, KIM S J, et al. Isolation, characterization, and abundance of filamentous members of Caldilineae in activated sludge[J]. The Journal of Microbiology, 2010,48(3):275-283. [24] FUERST J A. Intracellular compartmentation in planctomycetes[J]. Annual Review of Microbiology, 2005,59(1):299-328. [25] JO Y J, OH Y S, WOO S, et al. Metagenomic analysis of bacterial communities associated with four Ecklonia cava populations, including dokdo island population[J]. Toxicology and Environmental Health Sciences, 2019,11(1):11-18. [26] RAMITHA A X Y, CHAN X Y, YIN W Y, et al. Metagenomic analysis of microbial diversity of tropical sea water of georgetown coast, malaysia[J]. Life Science Journal, 2013,10(3):2392-2396. [27] VENTER J C, REMINGTON K, HEIDELBERG J F, et al. Environmental genome shotgun sequencing of the Sargasso Sea[J]. Science, 2004,304(5667):66-74. [28] YU K, ZHANG T. Metagenomic and metatranscriptomic analysis of microbial community structure and gene expression of activated sludge[J]. PLoS ONE, 2012,7(5):e38183. [29] CHEN S, CHENG H C, WYCKOFF K N, et al. Linkages of firmicutes and bacteroidetes populations to methanogenic process performance[J]. Journal of Industrial Microbiology Biotechnology, 2016,43(6):771-781. [30] VEKEMAN B, KERCKHOF F, CREMERS G, et al. New Methyloceanibacter diversity from North Sea sediments includes methanotroph containing solely the soluble methane monooxygenase[J]. Environmental Microbiology, 2016,18(12):4523-4536. [31] MIO T T K, TAKAO Y, SATOSHI H, et al. Methyloceanibacter caenitepidi gen. nov., sp. nov., a facultatively methylotrophic bacterium isolated from marine sediments near a hydrothermal vent[J]. International Journal of Systematic and Evolutionary Microbiology, 2014,64(2):462-468. [32] BLACKWELL N, PERKINS W, PALUMBO-ROE B, et al. Seasonal blooms of neutrophilic Betaproteobacterial Fe(Ⅱ) oxidizers and Chlorobi in iron-rich coal mine drainage sediments[J]. FEMS Microbiology Ecology, 2019,95(10):1-14. [33] LIU Z F, FRIGAARD N U, VOGL K, et al. Complete genome of ignavibacterium album, a metabolically versatile, flagellated, facultative anaerobe from the phylum chlorobi[J]. Frontiers in Microbiology, 2012,3:185.
点击查看大图
计量
- 文章访问数: 196
- HTML全文浏览量: 37
- PDF下载量: 18
- 被引次数: 0