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游离氨(FA)对SBR短程硝化过程微生物菌群结构及多样性的影响

吴代顺 常焕焕 陈翠忠 杨浩 侯红勋 孙洪伟

吴代顺, 常焕焕, 陈翠忠, 杨浩, 侯红勋, 孙洪伟. 游离氨(FA)对SBR短程硝化过程微生物菌群结构及多样性的影响[J]. 环境工程, 2021, 39(3): 82-89. doi: 10.13205/j.hjgc.202103012
引用本文: 吴代顺, 常焕焕, 陈翠忠, 杨浩, 侯红勋, 孙洪伟. 游离氨(FA)对SBR短程硝化过程微生物菌群结构及多样性的影响[J]. 环境工程, 2021, 39(3): 82-89. doi: 10.13205/j.hjgc.202103012
WU Dai-shun, CHANG Huan-huan, CHEN Cui-zhong, YANG Hao, HOU Hong-xun, SUN Hong-wei. EFFECTS OF FREE AMMONIA (FA) ON STRUCTURE AND DIVERSITY OF MICROFLORA IN SBR SHORT-CUT NITRIFICATION PROCESS[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(3): 82-89. doi: 10.13205/j.hjgc.202103012
Citation: WU Dai-shun, CHANG Huan-huan, CHEN Cui-zhong, YANG Hao, HOU Hong-xun, SUN Hong-wei. EFFECTS OF FREE AMMONIA (FA) ON STRUCTURE AND DIVERSITY OF MICROFLORA IN SBR SHORT-CUT NITRIFICATION PROCESS[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(3): 82-89. doi: 10.13205/j.hjgc.202103012

游离氨(FA)对SBR短程硝化过程微生物菌群结构及多样性的影响

doi: 10.13205/j.hjgc.202103012
详细信息
    作者简介:

    吴代顺(1976-),男,副教授,主要研究方向为水污染控制工程。765102712@qq.com

    通讯作者:

    孙洪伟(1976-),男,教授,主要研究方向为水体污染控制。12821306@qq.com

EFFECTS OF FREE AMMONIA (FA) ON STRUCTURE AND DIVERSITY OF MICROFLORA IN SBR SHORT-CUT NITRIFICATION PROCESS

  • 摘要: 为探究游离氨(FA)对硝化过程影响的机理,试验以人工模拟废水为研究对象,基于16S rRNA基因-Illumina MiSeq高通量测序技术,采用4组平行的SBR反应器(进水FA浓度分别控制为0.5,5,10,15 mg/L,分别记为R0.5、R5、R10和R15),探究了微生物在不同FA浓度条件下的群落组成和结构特征。结果表明:FA会显著影响系统内微生物菌群结构和功能。R0.5的α多样性指数(包括Chao1、ACE、Shannon和Simpson指数)在4组反应器中均为最大,说明R0.5的物种多样性最高,而R15的物种多样性最低。此外,在微生物门水平上,变形菌门Proteobacteria(45.9%~70.5%)和拟杆菌门Bacteroidetes(11.8%~41.3%)最具优势,且变形菌门(Proteobacteria)的相对丰度随着FA浓度升高而升高。在微生物属水平上,动胶菌属Zoogloea和陶厄氏菌属Thauera最具优势,且亚硝化单胞菌属(Nitrosomonas)和硝化螺旋菌属(Nitrospira)在R10中丰度明显高于其他3个系统。基于LEfSe分析,共获得了25个具有显著差异的微生物标记物,从而得到了各FA浓度条件下在微生物学分类水平上的菌群关键生物标记物。
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出版历程
  • 收稿日期:  2020-10-15
  • 网络出版日期:  2021-07-19

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