消化污泥序批式驯化中产气性能和微生物多样性变化

刘新媛; 胡文甲; 欧阳帆; 聂家民; 吴楠; 杨帆; 孔丝纺

doi:10.13205/j.hjgc.202103019

消化污泥序批式驯化中产气性能和微生物多样性变化

doi: 10.13205/j.hjgc.202103019

1. 天津农学院工程技术学院, 天津 300384;
2. 深圳信息职业技术学院交通与环境学院, 广东深圳 518172

基金项目:

天津市应用基础与前沿技术研究计划项目（16JCQNJCO8200）；天津农学院科研发展基金计划（20190115）；广东高校青年创新人才类项目（2017GkQNCX066）；深圳市科技计划（JCYJ20180307155011964）。

详细信息

作者简介:
刘新媛(1987-),女,博士,讲师,主要研究方向为有机废弃物资源化利用。liuxinyuan11@163.com

通讯作者:
欧阳帆(1984-),女,博士,讲师,主要研究方向为环境污染生物处理技术。ouyangfanily@163.com

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- 文章访问数: 138
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- 被引次数: 0
出版历程
- 收稿日期: 2020-02-27
- 网络出版日期: 2021-07-19

BIOGAS PRODUCTION AND MICROBIAL COMMUNITY SUCCESSION DURING SEQUENCING BATCH ACCLIMATIZATION OF DIGESTED SLUDGE

1. College of Engineering and Technology, Tianjin Agricultural University, Tianjin 300384, China;
2. Traffic and Environment Department, Shenzhen Institute & Information Technology, Shenzhen 518172, China

摘要

摘要: 利用序批式运行方法探索低活性厌氧接种污泥对新底物的适应过程。研究发现，接种污泥经过1个批次驯化后，后续批次的甲烷产量维持稳定，说明首批次培养实现了有效的代谢调整。分析各发酵批次的pH值和产甲烷动力学参数发现，随着驯化批次的增加，接种污泥产甲烷的延迟期缩短、产甲烷速率升高，且各批次初期酸化程度降低，说明了多批次驯化促进污泥对底物的适应能力。高通量测序技术分析驯化前后微生物群落结构可知，3个批次驯化后污泥菌群的丰富度和多样性均降低。驯化筛选出以Paludibacter属（相对丰度为52.3%）和Methanosaeta属（相对丰度为72.31%）为优势菌属的群落结构。
- 消化污泥 /
- 产甲烷 /
- 驯化培养 /
- 厌氧发酵 /
- 菌群结构
Abstract: This article investigated the adaptability changes of slight-activated seed sludge to new substrate during anaerobic sequencing batch acclimatization. Results showed that the seed sludge had stable methane production ability in the second and third batch after the acclimatization in the first batch, indicating that the effective metabolic adjustment had been obtained in the first batch. According to the results of the pH value and kinetic parameters for methane production in each batch, it was found the delay period for methane production was shortened, the methane production rate was increased and the acidification degree in the initial period of each batch was reduced with the acclimated batch. The results indicated that the adaptability of seed sludge to substrate was gradually enhanced. The microbial community structure before and after sequencing batch culture were detected by high throughput sequencing technology. Results showed that the richness and diversity of acclimated sludge reduced after the three times batch culture. The dominant bacterial genus of Paludibacter with relative abundance of 52.3% and the dominant archaeal genus of Methanosaeta with relative abundance of 72.31%, were selected during the acclimatization.
- digested sludge /
- methane production /
- acclimatization /
- anaerobic digestion /
- community structure

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参考文献(24)

[1]	MATHERI A N, NDIWENI S N, BELAID M, et al. Optimising biogas production from anaerobic co-digestion of chicken manure and organic fraction of municipal solid waste[J]. Renewable and Sustainable Energy Reviews, 2017, 80:756-764.
[2]	刘新媛,肖娟,聂家民,等. 鸡粪和餐厨垃圾中温厌氧发酵产甲烷特征及动力学[J]. 中国沼气,2019,37(5):15-20.
[3]	袁悦. 污泥与餐厨垃圾共消化系统启动策略[J]. 环境工程,2018,36(11):137-140 ,75.
[4]	冯磊,徐杰,李润东. 餐厨垃圾中温厌氧发酵接种污泥的驯化研究[J]. 可再生能源,2013,31(10):98-102 ,108.
[5]	裴梦富,强虹,杨祎楠,等. 利用逐级提高进料浓度的方法启动完全混合反应器处理鸡粪[J]. 环境工程学报,2018,12(6):1825-1832.
[6]	WANG M, SUN X L, LI P F, et al. A novel alternate feeding mode for semi-continuous anaerobic co-digestion of food waste with chicken manure[J]. Bioresource Technology, 2014, 164:309-314.
[7]	KURADE M B, SAHA S, KIM J R, et al. Microbial community acclimatization for enhancement in the methane productivity of anaerobic co-digestion of fats, oil, and grease[J]. Bioresource Technology, 2020, 296:122294.
[8]	ZHANG H, WU J W, GAO L J, et al. Aerobic deterioration of corn stalk silage and its effect on methane production and microbial community dynamics in anaerobic digestion[J]. Bioresource Technology, 2018, 250:828-837.
[9]	王旭辉,徐鑫,宝哲,等. 高通量测序分析玉米秸秆与牛粪联合发酵阶段微生物多样性变化[J]. 食品与发酵工业,2019,45(3):47-55.
[10]	吴坤君, 龚佩瑜, 盛承发. 昆虫多样性参数的测定和表达[J]. 昆虫, 2005(3):338-340.
[11]	孟颖,唐明跃,孙芳青,等. 餐厨、果蔬与鸡粪多元混合物料厌氧消化实验研究[J]. 中国沼气,2013,31(4):12-16 ,48.
[12]	ROSALINDA C AND SIMÓN G M. Start-up of dry semi-continuous OFMSW fermentation for methane production[J]. Biomass and Bioenergy, 2020, 136:105544.
[13]	DING H B, LIU X Y, STABNIKOVA O, et al. Effect of protein on biohydrogen production from starch of food waste[J]. Water Science & Technology, 2008, 57(7):1031-1036.
[14]	LIU C, LI H, ZHANG Y Y, et al. Evolution of microbial community along with increasing solid concentration during high-solids anaerobic digestion of sewage sludge[J]. Bioresource Technology, 2016, 216:87-94.
[15]	KADNIKOV V V, MARDANOV A V, BELETSKY A V, et al. Genome of the candidate phylum Aminicenantes bacterium from a deep subsurface thermal aquifer revealed its fermentative saccharolytic lifestyle[J]. Extremophiles, 2019, 23(2):189-200.
[16]	TAO Y, ERSAHIN M E, GHASIMI D S M, et al. Biogas productivity of anaerobic digestion process is governed by a core bacterial microbiota[J]. Chemical Engineering Journal, 2020, 380:122425.
[17]	PAN J T, MA J Y, ZHAI L M, et al. Enhanced methane production and syntrophic connection between microorganisms during semi-continuous anaerobic digestion of chicken manure by adding biochar[J]. Journal of Cleaner Production, 2019, 240:118178.
[18]	QIU Y L, KUANG X Z, SHI X S, et al. Paludibacter jiangxiensis sp. nov., a strictly anaerobic, propionate-producing bacterium isolated from rice paddy field[J]. Archives of Microbiology, 2014, 196(3):149-155.
[19]	徐恒,汪翠萍,颜锟,等. 颗粒型厌氧生物膜改善高氢分压下丙酸降解抑制研究[J]. 中国环境科学,2016,36(5):1435-1441.
[20]	IMACHI H, SAKAI S M[M]. Bergey's Manual of Systematics of Archaea and Bacteria, 2015:1-4.
[21]	王洋,李海红,常华.餐厨垃圾与固体废物共消化产气效果研究[J].化学工程,2019,47(3):1-6 ,18.
[22]	KERSTIN M, SHARIF A, MARIAN K. Beneficial effects of intermittent feedstock management on biogas and methane production[J]. Bioresource Technology, 2020, 304:123004.
[23]	XING B S, HAN Y, WANG X C, et al. Cow manure as additive to a DMBR for stable and high-rate digestion of food waste:performance and microbial community[J]. Water Research, 2020, 168:115099.
[24]	朱铁群,李凯慧,张杰. 活性污泥驯化的微生物生态学原理[J]. 微生物学通报,2008,35(6):939-943.