CSCD来源期刊
中国科技核心期刊
RCCSE中国核心学术期刊
JST China 收录期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

共存Zn(Ⅱ)抑制Pannonibacter phragmitetus BB还原Cr(Ⅵ)的分子机理

唐佳琪 杨卫春 杨志辉 廖骐

唐佳琪, 杨卫春, 杨志辉, 廖骐. 共存Zn(Ⅱ)抑制Pannonibacter phragmitetus BB还原Cr(Ⅵ)的分子机理[J]. 环境工程, 2021, 39(4): 36-41. doi: 10.13205/j.hjgc.202104007
引用本文: 唐佳琪, 杨卫春, 杨志辉, 廖骐. 共存Zn(Ⅱ)抑制Pannonibacter phragmitetus BB还原Cr(Ⅵ)的分子机理[J]. 环境工程, 2021, 39(4): 36-41. doi: 10.13205/j.hjgc.202104007
TANG Jia-qi, YANG Wei-chun, YANG Zhi-hui, LIAO Qi. MOLECULAR MECHANISM OF Cr(Ⅵ) REDUCTION INHIBITION BY PANNONIBACTER PHRAGMITETUS BB WITH CO-EXISTING Zn(Ⅱ)[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(4): 36-41. doi: 10.13205/j.hjgc.202104007
Citation: TANG Jia-qi, YANG Wei-chun, YANG Zhi-hui, LIAO Qi. MOLECULAR MECHANISM OF Cr(Ⅵ) REDUCTION INHIBITION BY PANNONIBACTER PHRAGMITETUS BB WITH CO-EXISTING Zn(Ⅱ)[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(4): 36-41. doi: 10.13205/j.hjgc.202104007

共存Zn(Ⅱ)抑制Pannonibacter phragmitetus BB还原Cr(Ⅵ)的分子机理

doi: 10.13205/j.hjgc.202104007
基金项目: 

国家重点研发项目"多污染物协同修复材料研发及长效安全适用性评估"(2018YFC1802204)。

详细信息
    作者简介:

    唐佳琪(1995-),女,硕士研究生,主要研究方向为Cr (Ⅵ)污染微生物治理技术。1316085669@qq.com

    通讯作者:

    廖骐(1979-),男,博士,副教授,主要研究方向为重金属污染场地微生物生态及修复技术。liaoqi@csu.edu.cn

MOLECULAR MECHANISM OF Cr(Ⅵ) REDUCTION INHIBITION BY PANNONIBACTER PHRAGMITETUS BB WITH CO-EXISTING Zn(Ⅱ)

  • 摘要: 通过电子传递体系活性、酶活性以及实时定量PCR技术,揭示共存Zn(Ⅱ)延迟Pannonibacter phragmitetus BB(BB)生长,抑制Cr(Ⅵ)还原的分子机理。结果表明:共存Zn(Ⅱ)抑制BB菌还原Cr(Ⅵ)主要表现为胞内和胞外抑制,共存Zn(Ⅱ)通过降低电子传递体系活性和细胞色素c氧化酶活性,抑制其胞外Cr(Ⅵ)还原;共存Zn(Ⅱ)通过降低铬转运效率的方式,抑制其胞内Cr(Ⅵ)还原。SOD、CAT、POD、GST 4种抗氧化酶活性的研究表明:共存Zn(Ⅱ)引起的胞内氧化压力较小,对BB菌造成的毒性较低,从侧面印证了共存Zn(Ⅱ)对BB菌生长的延迟作用。
  • [1] 汤文帅,方雨虹,陈涛,等. 电镀废水原位制备Ni(Zn)Cr混合氧化物/磁性石墨烯复合材料及其电催化性能研究[J]. 电镀与涂饰, 2019, 38(13):689-696.
    [2] GHORPADE A, AHAMMED M M. Water treatment sludge for removal of heavy metals from electroplating wastewater[J]. Environmental Engineering Research, 2017, 23(1):92-98.
    [3] KUMAR V, DWIVEDI S K. Hexavalent chromium stress response, reduction capability and bioremediation potential of Trichoderma sp. isolated from electroplating wastewater[J]. Ecotoxicology and Environmental Safety, 2019, 185:109734.
    [4] TAN H, WANG C, ZENG G Q, et al. Bioreduction and biosorption of Cr(Ⅵ) by a novel Bacillus sp. CRB-B1 strain[J]. Journal of Hazardous Materials, 2020, 386:121628.
    [5] KUMAR V, DWIVEDI S K. Hexavalent chromium reduction ability and bioremediation potential of Aspergillus flavus CR500 isolated from electroplating wastewater[J]. Chemosphere, 2019, 237:124567.
    [6] MA L L, XU J M, CHEN N, et al. Microbial reduction fate of chromium (Cr) in aqueous solution by mixed bacterial consortium[J]. Ecotoxicology and Environmental Safety, 2019, 170:763-770.
    [7] BANERJEE S, MISRA A, CHAUDHURY S, et al. A Bacillus strain TCL isolated from Jharia coalmine with remarkable stress responses, chromium reduction capability and bioremediation potential[J]. Journal of Hazardous Materials, 2019, 367:215-223.
    [8] ELANGOVAN R, PHILIP L, CHANDRARAJ K. Hexavalent chromium reduction by free and immobilized cell free extract of Arthrobacter rhombi-RE[J]. Applied Biochemistry and Biotechnology, 2010, 160(1):81-97.
    [9] BAI Y N, LU Y Z, SHEN N, et al. Investigation of Cr(Ⅵ) reduction potential and mechanism by Caldicellulosiruptor saccharolyticus under glucose fermentation condition[J]. Journal of Hazardous Materials, 2018, 344:585-592.
    [10] GE S M, SHI C G. Simultaneous Cr(Ⅵ) reduction and Zn(Ⅱ) biosorption by Stenotrophomonas sp. and constitutive expression of related genes[J]. Biotechnology Letters, 2016, 38(5):877-884.
    [11] 魏蓝. 土壤微生物对六价铬的还原及稳定化效果研究[D]. 苏州:苏州科技大学, 2017.
    [12] WAN R, CHEN Y G, ZHENG X, et al. Effect of CO2 on microbial denitrification via inhibiting electron transport and consumption[J]. Environmental Science Technology, 2016, 50(18):9915-9922.
    [13] 朱文杰. Leucobacter sp.CRB1菌还原铬(Ⅵ)的机理及其在铬渣解毒中的应用[D]. 长沙:中南大学, 2008.
    [14] ZHAO S Y, SU X X, WANG Y Y, et al. Copper oxide nanoparticles inhibited denitrifying enzymes and electron transport system activities to influence soil denitrification and N2O emission[J]. Chemosphere, 2020, 245:125394.
    [15] VITI C, MARCHI E, DECOROSI F, et al. Molecular mechanisms of Cr(Ⅵ) resistance in bacteria and fungi[J]. FEMS Microbiology Reviews, 2014, 38(4):633-659.
    [16] CHAI L Y, DING C L, LI J W, et al. Multi-omics response of Pannonibacter phragmitetus BB to hexavalent chromium[J]. Environmental Pollution, 2019, 249:63-73.
    [17] THATOI H, DAS S, MISHRA J, et al. Bacterial chromate reductase, a potential enzyme for bioremediation of hexavalent chromium:a review[J]. Journal of Environmental Management, 2014, 146:383-399.
    [18] KANNT A, OSTERMANN T, MULLER H, et al. Zn2+ binding to the cytoplasmic side of Paracoccus denitrificans cytochrome c oxidase selectively uncouples electron transfer and proton translocation[J]. FEBS Letters, 2001, 503(2/3):142-146.
    [19] KAPPLER U, DAVENPORT K, BEATSON S, et al. Complete genome sequence of the facultatively chemolithoautotrophic and methylotrophic alpha Proteobacterium Starkeya novella type strain (ATCC 8093(T))[J]. Standards in Genomic Sciences, 2012, 7(1):44-58.
    [20] 韩倩. 亚硝酸还原酶产生菌的筛选、发酵优化以及酶学性质研究[D]. 广州:华南理工大学, 2015.
    [21] HUO Y Y, CHENG H, HAN X F, et al. Complete Genome Sequence of Pelagibacterium halotolerans B2(T)[J]. Journal of Bacteriology, 2012, 194(1):197-198.
    [22] XI J, SHENG X F, HE L Y. Draft Genome Sequence of Rhizobium sp. H41, a Rock-Weathering Bacterium from a Weathered Rock Surface[J]. Microbiology Resource Announcements, 2014, 2(6):e01127-14.
    [23] CHAI L Y, DING C L, TANG C J, et al. Discerning three novel chromate reduce and transport genes of highly efficient Pannonibacter phragmitetus BB:from genome to gene and protein[J]. Ecotoxicology and Environmental Safety, 2018, 162:139-146.
    [24] 周思敏,董兰岚,何元,等. ChrA基因在大肠杆菌中的表达及其抗铬特性[J]. 南方医科大学学报, 2017, 37(10):1290-1295.
    [25] XU X J, XIA L, CHEN W L, et al. Detoxification of hexavalent chromate by growing Paecilomyces lilacinus XLA[J]. Environmental Pollution, 2017, 225:47-54.
    [26] KHAN S, LV J, IQBAL A, et al. Morphophysiological and transcriptome analysis reveals a multiline defense system enabling cyanobacterium Leptolyngbya strain JSC-1 to withstand iron induced oxidative stress[J]. Chemosphere, 2018, 200:93-105.
    [27] SATAPUTE P, PAIDI M K, KURJOGI M, et al. Physiological adaptation and spectral annotation of Arsenic and Cadmium heavy metal-resistant and susceptible strain Pseudomonas taiwanensis[J]. Environmental Pollution, 2019, 251:555-563.
  • 加载中
计量
  • 文章访问数:  393
  • HTML全文浏览量:  45
  • PDF下载量:  21
  • 被引次数: 0
出版历程
  • 收稿日期:  2020-07-13
  • 网络出版日期:  2021-07-21

目录

    /

    返回文章
    返回