外环境温度对污泥堆肥过程中抗生素抗性基因的影响

张爱生; 曲俊蓉; 许斌; 杨晓伟; 邵艳秋; 付艳丽; 李博恩; 朱英

doi:10.13205/j.hjgc.202105014

外环境温度对污泥堆肥过程中抗生素抗性基因的影响

doi: 10.13205/j.hjgc.202105014

1. 齐鲁工业大学(山东省科学院)新材料研究所, 济南 250014;
2. 青岛娄山河水务资源有限公司, 山东青岛 266000;
3. 青岛市团岛污水处理厂, 山东青岛 266000

基金项目:

山东省重大科技创新项目"大宗工业固废协同互补生产宽温度窗口SCR脱硝催化剂的关键技术与装备研究"（2019JZZY020305）；底泥重金属污染治理关键技术与工程示范（2018YFJH0902）；齐鲁工业大学（山东省科学院）科教产项目"固体废弃物无害化、减量化与资源化关键技术研究"（2020KJC-ZD12）。

详细信息

作者简介:
张爱生(1994-),男,硕士研究生,主要研究方向为固体废物资源化。1185161721@qq.com

通讯作者:
朱英(1978-),女,博士,研究员,主要研究方向为固体废弃物处理处置及资源化利用。zhuyingee@163.com

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出版历程
- 收稿日期: 2020-07-22
- 网络出版日期: 2022-01-17

EFFECTS OF AMBIENT TEMPERATURE ON ANTIBIOTIC RESISTANCE GENES IN SLUDGE COMPOSTING

1. Institute of New Materials, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China;
2. Tsingtao Loushanhe Water Resources Co., Ltd, Qingdao 266000, China;
3. Tsingtao Tuandao Sewage Treatment Plant, Qingdao 266000, China

摘要

摘要: 为研究外环境温度对污泥堆肥过程中抗生素抗性基因的影响，选取市政污水处理厂脱水污泥、花生壳和堆肥返混料作为堆肥材料，采用室内静态堆肥加翻堆方式，通过测定30，50，55℃下堆肥过程中堆体理化性质和抗性基因（tetA、tetQ、sul2、sul3）及一类整合子丰度，分析3种外环境温度对污泥堆肥过程中抗生素抗性基因变化的影响。结果表明：50，55℃外环境温度堆肥条件可有效降解污泥堆肥过程中tetA、tetQ、sul2、sul3抗性基因丰度，在堆肥结束时总ARGs降解率达到66%~83%，而30℃外环境堆肥结束时，总ARGs降解率为30%左右。移动遗传元件IntI1的丰度变化与ARGs的丰度变化趋势相似，堆肥结束时50℃和55℃的外环境温度对堆体中IntI1的降解率达到92%以上，高于30℃条件71.9%的降解率，其原因可能是适当高的外环境温度会抑制堆体中部分微生物的生长代谢，减少了IntI1的传播，进而影响ARGs的传播。30℃外环境温度堆肥条件下，堆体在第36天达到腐熟，发芽指数为59.80%；而50℃和55℃堆肥条件下，在第28天即可达到腐熟，发芽指数达到60%，腐熟速度明显高于30℃条件。研究表明，50℃和55℃的外环境温度堆肥条件和30℃相比可以缩短腐熟所需时间，且对tetA、tetQ、sul2、sul3抗性基因及IntI1丰度有很好的降解效果。因此，堆肥时适当提高外环境温度对污泥发酵和其中ARGs有一定的影响，理论上具有一定的可行性。
- 污泥堆肥 /
- 抗生素抗性基因 /
- 外环境温度
Abstract: To study the effect of ambient temperature on the changes of antibiotic resistance gene variation in sludge composting process, municipal sewage treatment plant sludge dewatering, peanut shells and compost backmixing material were selected as composting materials. The effects of the three external environment temperature on changes of antibiotic resistances genes in sludge composting process were analyzed and compared by measuring the physical and chemical properties of composted materials and resistance genes(tetA、tetQ、sul2、sul3) and class integron(IntI1) abundance in the composting process at 30℃, 50℃ and 55℃ by the indoor static composting and turning method. The results showed that the abundance of tetA, tetQ, sul2 and sul3 resistance genes in the process of sludge composting at ambient temperatures outside 50℃ and 55℃ could be effectively reduced, and the degradation rate of total ARGs reached 66%~83% at the end of composting, while the degradation rate of total ARGs was about 30% at the end of composting at external temperature of 30℃. The degradation rate of mobile genetic elements IntI1 at the end of the composting at 50℃ and 55℃ was 92% above, higher than that of at 30℃ (71.9%). The reason may be that proper high ambient temperature inhibited the growth and metabolism of some microbial in the pile and reduced the spread of IntI1, and then affected the spread of the ARGs. The compost at ambient temperature of 30℃ reached maturity on the 36th day, and the germination index was 59.80%. The compost at 50℃ and 55℃ reached maturity on the 28th day, the germination index reached 60%, and the maturity rate was significantly higher than that at 30℃. The study showed that composting conditions at ambient temperatures of 50℃ and 55℃ could shorten the time required for composting compared with that of 30℃, and had a good degradation effect on tetA, tetQ, sul2, sul3 resistance genes and IntI1 abundance. Therefore, appropriately increasing the ambient temperature during the composting process had certain effect on sludge fermentation and ARGs.
- sludge composting /
- antibiotic resistance genes /
- environment temperature

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参考文献(16)

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