耐热复合菌系强化全程高温堆肥快速处理餐厨垃圾

宋彩红; 齐辉; 魏自民; 夏训峰

doi:10.13205/j.hjgc.202105015

耐热复合菌系强化全程高温堆肥快速处理餐厨垃圾

doi: 10.13205/j.hjgc.202105015

1. 聊城大学生命科学学院, 山东聊城 252000;
2. 中国环境科学研究院, 北京 100012;
3. 东北农业大学生命科学学院, 哈尔滨 150030

基金项目:

国家水体污染控制与治理科技重大专项（2015ZX07103-007-03）；国家自然科学基金（51978131）。

详细信息

作者简介:
宋彩红(1986-),女,博士,讲师,主要研究方向为固废资源化。

通讯作者:
夏训峰,男,博士,研究员,主要研究方向为村镇环境综合治理。xiaxunfengg@sina.com.cn

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出版历程
- 收稿日期: 2020-08-05
- 网络出版日期: 2022-01-17

HIGH-SPEED TREATMENT OF FOOD WASTE BY CONTINUOUS HIGH-TEMPERATURE COMPOSTING ENHANCED BY THERMOPHILIC MICROBIAL CONSORTIUM

1. Life Science College, Liaocheng University, Liaocheng 252000, China;
2. Chinese Research Academy of Environmental Science, Beijing 100012, China;
3. School of Life Sciences, Northeast Agricultural University, Harbin 150030, China

摘要

摘要: 为进一步提升全程高温堆肥效率，经筛选和高温驯化，获得高有机质降解效率的耐热复合菌系（TMC），设置全程高温接种TMC堆肥组（T1）、全程高温堆肥组（T2）和常温堆肥组（T3），通过理化指标、粗脂肪和粗蛋白含量、GI等指标的检测和优势细菌演替规律分析，以揭示TMC对全程高温堆肥工艺的影响。结果显示：堆肥结束后有机质含量、C/N、粗脂肪和蛋白含量降幅顺序均为T1>T2>T3（P<0.05），且两两处理之间均具有显著性差异，证明TMC可缩短全程高温堆肥进程。堆肥第14天，T1、T2处理的GI值分别为110%和99%，T3处理仅为80%，表明全程高温堆肥可加速植物毒性物质降解，显著提高堆肥品质，而TMC接种可进一步促进堆肥无害化。PCR-DGGE结果表明：T1、T2处理均显著提高了耐热细菌和耐热木质纤维素降解菌多样性，且并未降低嗜中温木质纤维素降解菌多样性；2类降解菌协同配合实现木质纤维素的更快降解，有利于缩短堆肥进程。综上所述，TMC接种可显著提高全程高温堆肥效率、提升堆肥品质。
- 耐热复合菌系 /
- 全程高温 /
- 快速堆肥 /
- 餐厨垃圾
Abstract: In order to further improve the disposing efficiency of continuous high-temperature composting disposing food waste, we obtained thermophilic microbial consortium (TMC) degrading organic matter efficiently, by screening and high-temperature domestication. Three treatments, including continuous high-temperature composting with (T1) and without TMC inoculation (T2) and natural composting (T3) were set. The influence of TMC inoculation on continuous high-temperature composting process was revealed by comparison of physical and chemical indexes, crude fat and protein content and GI index and analysis of dominant bacterial succession law. Results showed that the decreased levels of organic matter content, C/N, crude fat and protein content were all in order of T1>T2>T3 after composting. The above indicators were significantly different among T1, T2 and T3 (P<0.05). These results confirmed that inoculation of TMC could accelerate the continuous high-temperature composting process and improve composting efficiency. On the 14th day of composting, the GI was 110% and 99% in T1 and T2 treatment respectively, but that of T3 treatment was only 80%, indicating that the continuous high-temperature composting could accelerate the degradation of phytotoxic substances and significantly improve compost quality. TMC inoculation could further promote harm-free compost. PCR-DGGE results showed that the diversities of thermophilic or heat-resistant bacteria and heat-resistant lignocellulose-degrading bacteria increased remarkably in T1 and T2 treatment. At the same time, mesophilic lignocellulose-degrading bacterial diversity did not decrease. Two kinds of bacteria cooperated to achieve faster degradation of lignocellulose, which was helpful in shortening food waste composting process. In conclusion, TMC inoculation could significantly improve the efficiency of continuous high-temperature composting and food waste compost quality.
- thermophilic microbial consortium /
- continuous high-temperature /
- high-speed composting /
- food waste

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参考文献(25)

[1]	KENG Z X, CHONG S, NG C G, et al. Community-scale composting for food waste:a life-cycle assessment-supported case study[J]. Journal of Cleaner Production, 2020, 261:121220.
[2]	NAKASAKI K, HIRAI H, MIMOTO H, et al. Succession of microbial community during vigorous organic matter degradation in the primary fermentation stage of food waste composting[J]. Science of the Total Environment, 2019, 671:1237-1244.
[3]	石文军. 全程高温好氧堆肥快速降解城市生活垃圾及其腐熟度判定[D]. 长沙:湖南大学, 2010.
[4]	薛兆骏, 彭永臻, 王鹏鹞, 等. 自发热持续高温好氧堆肥碳、氮、腐植酸变化过程[J]. 中国环境科学, 2018, 38(11):4094-4098.
[5]	薛兆骏, 周国亚, 俞肖峰, 等. 超高温自发热好氧堆肥工艺处理剩余污泥[J]. 中国环境科学, 2017, 37(9):3399-3406.
[6]	王晓诚, 郭颖, 颜开红. 超高温自发热好氧堆肥工艺处理生活垃圾的探究[J/OL]. 环境工程, https://kns.cnki.net/kcms/detail/11.2097.X.20200616.1039.006.html.
[7]	石文军, 杨朝晖, 肖勇,等. 全程高温好氧堆肥快速降解城市生活垃圾[J]. 环境科学学报, 2009,29(10):2126-2133.
[8]	SONG C H, LI M X, JIA X, et al. Comparison of bacterial community structure and dynamics during the thermophilic composting of different types of solid wastes:anaerobic digestion residue, pig manure and chicken manure[J]. Microbial Biotechnology, 2014, 7(5):424-433.
[9]	SONG C H, ZHANG Y L, XIA X F, et al. Effect of inoculation with a microbial consortium that degrades organic acids on the composting efficiency of food waste[J]. Microbial Biotechnology, 2018, 11(6):1124-1136.
[10]	宋彩红, 张亚丽, 李鸣晓, 等. 抗酸化微生物复合菌系对餐厨垃圾堆肥腐殖质组分光谱学性质的影响[J].光谱学与光谱分析, 2019, 39(11):3533-3539.
[11]	BRABSON J A. The kjeldahl method for organic nitrogen[J]. Journal of AOAC International, 1966, 49(2):481-481.
[12]	HAWTHORNE S B, GRABANSKI C B, MARTIN E, et al. Comparisons of Soxhlet extraction, pressurized liquid extraction, supercritical fluid extraction and subcritical water extraction for environmental solids:recovery, selectivity and effects on sample matrix[J]. Journal of Chromatography A, 2000, 892(1):421-433.
[13]	SAIDPULLICINO D, ERRIQUENS F G, GIGLIOTTI G, et al. Changes in the chemical characteristics of water-extractable organic matter during composting and their influence on compost stability and maturity[J]. Bioresource Technology, 2007, 98(9):1822-1831.
[14]	SONG C H, LI M X, XI B D, et al. Characterisation of dissolved organic matter extracted from the bio-oxidative phase of co-composting of biogas residues and livestock manure using spectroscopic techniques[J]. International Biodeterioration & Biodegradation, 2015, 103:38-50.
[15]	HOSSEINI S M, AZIZ H A. Evaluation of thermochemical pretreatment and continuous thermophilic condition in rice straw composting process enhancement[J]. Bioresource Technology, 2013, 133:240-247.
[16]	TRAN Q N, MIMOTO H, KOYAMA M, et al. Lactic acid bacteria modulate organic acid production during early stages of food waste composting[J]. Science of the Total Environment, 2019, 687:341-347.
[17]	GARCIA-GÓMEZ A, ROIG A, BERNAL M P. Composting of the solid fraction of olive mill wastewater with olive leaves:organic matter degradation and biological activity[J]. Bioresource Technology, 2003, 86(1):59-64.
[18]	AWASTHI M K, CHEN H Y, WANG Q, et al. Succession of bacteria diversity in the poultry manure composted mixed with clay:Studies upon its dynamics and associations with physicochemical and gaseous parameters[J]. Bioresource Technology, 2018, 267:618-625.
[19]	KOYAMA M, NAGAO N, SYUKRI F, et al. Effect of temperature on thermophilic composting of aquaculture sludge:NH₃ recovery, nitrogen mass balance, and microbial community dynamics[J]. Bioresource Technology, 2018, 265:207-213.
[20]	TORTOSA G, CASTELLANOHINOJOSA A, CORREAGALEOTE D, et al. Evolution of bacterial diversity during two-phase olive mill waste ("alperujo") composting by 16S rRNA gene pyrosequencing[J]. Bioresource Technology, 2017, 224:101-111.
[21]	MA C F, LO P K, XU J Q, et al. Molecular mechanisms underlying lignocellulose degradation and antibiotic resistance genes removal revealed via metagenomics analysis during different agricultural wastes composting[J]. Bioresource Technology, 2020, 314:123731.
[22]	AKYOL Ç, INCE O, INCE B. Crop-based composting of lignocellulosic digestates:Focus on bacterial and fungal diversity[J]. Bioresource Technology, 2019, 288:121549.
[23]	DING J L, WEI D, AN Z Z, et al. Succession of the bacterial community structure and functional prediction in two composting systems viewed through metatranscriptomics[J]. Bioresource Technology, 2020, 313:123688.
[24]	KARADAG D, ÖZKAYA B, ÖLMEZ E, et al. Profiling of bacterial community in a full-scale aerobic composting plant[J]. International Biodeterioration & Biodegradation, 2013, 77:85-90.
[25]	SONG C H, LI M X, QI H, et al. Impact of anti-acidification microbial consortium on carbohydrate metabolism of key microbes during food waste composting[J]. Bioresource Technology, 2018, 259:1-9.