饮用水活性炭净水器对抗生素抗性基因的去除效果

徐兰; 周振超; 朱琳; 刘扬; 帅馨怡; 林泽俊; 陈红

doi:10.13205/j.hjgc.202106005

饮用水活性炭净水器对抗生素抗性基因的去除效果

doi: 10.13205/j.hjgc.202106005

浙江大学环境与资源学院环境技术研究所, 杭州 310058

基金项目:

国家自然科学基金（21677121，21876147）

详细信息

作者简介:
徐兰(1994-),女,硕士研究生,主要研究方向为饮用水抗生素抗性基因污染及控制。xlan@zju.edu.cn

通讯作者:
陈红(1969-),女,博士,教授,主要研究方向为新型污染物的控制技术。chen_hong@zju.edu.cn

计量
- 文章访问数: 234
- HTML全文浏览量: 37
- PDF下载量: 6
- 被引次数: 0
出版历程
- 收稿日期: 2020-10-15
- 网络出版日期: 2022-01-18

REMOVAL EFFICIENCY OF ANTIBIOTIC RESISTOME IN ACTIVATED CARBON DRINKING WATER PURIFIERS

Institute of Environmental Technology, College of Environmental and Resource Sciences, Zhejiang University, Hangzhou 310058, China

摘要

摘要: 为了评估不同类型家用饮用水活性炭净水器对常规水质及新型污染物抗生素抗性基因（antibiotic resistance genes， ARGs）的净化能力，购置了6种具有不同滤芯结构的家用活性炭净水器，对常规水质指标及抗性基因的动态变化（80 d）进行跟踪调查。实验结果显示：活性炭净水器能长期有效去除余氯，但对有机物的去除效果随着时间推移而减弱。活性炭的物理性质对净水性能影响较大，比表面积越大，吸附性能就越强，去除有机物的能力就越高。采用qPCR定量分析了饮用水中5种抗性基因，绝对丰度最高的是sul1和tetA，活性炭净水器出水中抗性基因的绝对丰度随时间增加逐渐上升，48~80 d时出水中抗性基因绝对丰度达到较高水平。具有刚性孔隙和渐紧过滤结构的烧结活性炭能对抗性基因起到一定的控制效果。水中有机物质和Ⅰ类整合子intI1在抗性基因的增殖与传播中发挥了重要作用。
- 饮用水 /
- 活性炭净水器 /
- 抗生素抗性基因 /
- 荧光定量PCR
Abstract: In order to evaluate the water purification capacities of household activated carbon drinking water purifiers and the behavioral characteristics of antibiotic resistance genes(ARGs), six kinds of household activated carbon water purifiers with different filter structures were purchased in this study. The dynamic changes of conventional water quality indicators and ARGs were tracked for 80 days. The research results showed that activated carbon drinking water purifiers could effectively remove residual chlorine for a long period of time. The removal effect of organic matter deteriorated with time. The physical properties of activated carbon had a great impact on water purification performance. Large specific surface area helped to remove organics. Five ARGs were quantitatively analyzed by qPCR, and the absolute abundances of sul1 and tetA were the highest. The absolute abundances of ARGs in the filtered water gradually increased with time, and reached a high level in 48~80 days in general. Sintered activated carbon with rigid pores and gradually-tight filter structure could play a certain control effect on ARGs. Organic matter and intI1 in water played an important role in the proliferation and transmission of ARGs.
- drinking water /
- activated carbon purifiers /
- antibiotic resistance genes /
- real-time quantitative PCR

HTML全文

参考文献(22)

[1]	TANG J,WANG L L,XI Y F,et al.A three-year survey of the antimicrobial resistance of microorganisms at a Chinese hospital[J].Experimental and Therapeutic Medicine,2016,11(3):731-736.
[2]	FANG H,WANG H F,CAI L,et al.Prevalence of antibiotic resistance genes and bacterial pathogens in long-term manured greenhouse soils as revealed by metagenomic survey[J].Environmental Science & Technology,2015,49(2):1095-1104.
[3]	MANAIA C M,ROCHA J,SCACCIA N,et al.Antibiotic resistance in wastewater treatment plants:tackling the black box[J].Environment International,2018,115:312-324.
[4]	STOLL C,SIDHU J P S,TIEHM A,et al.Prevalence of clinically relevant antibiotic resistance genes in surface water samples collected from Germany and Australia[J].Environmental Science & Technology,2012,46(17):9716-9726.
[5]	JIANG L,HU X L,XU T,et al.Prevalence of antibiotic resistance genes and their relationship with antibiotics in the Huangpu River and the drinking water sources,Shanghai,China[J].Science of the Total Environment,2013,458-460:267-272.
[6]	ZHENG J,CHEN T,CHEN H,et al.Antibiotic resistome promotion in drinking water during biological activated carbon treatment:is it influenced by quorum sensing?[J].Science of the Total Environment,2018,612:1-8.
[7]	XU L K,OUYANG W Y,QIAN Y Y,et al.High-throughput profiling of antibiotic resistance genes in drinking water treatment plants and distribution systems[J].Environmental Pollution,2016,213:119-126.
[8]	WU C C,GHOSH S,MARTIN K J,et al.The microbial colonization of activated carbon block point-of-use (PoU) filters with and without chlorinated phenol disinfection by-products[J].Environmental Science-Water Research & Technology,2017,3(5):830-843.
[9]	KOROTTA-GAMAGE S M,SATHASIVAN A.A review:potential and challenges of biologically activated carbon to remove natural organic matter in drinking water purification process[J].Chemosphere,2017,167:120-138.
[10]	COOK D,NEWCOMBE G.Comparison and modeling of the adsorption of two microcystin analogues onto powdered activated carbon[J].Environmental technology,2008,29(5):525-534.
[11]	LO S F,WANG S Y,TSAI M J,et al.Adsorption capacity and removal efficiency of heavy metal ions by Moso and Ma bamboo activated carbons[J].Chemical Engineering Research and Design,2012,90(9):1397-1406.
[12]	CHAIDEZ C,GERBA C P.Comparison of the microbiologic quality of point-of-use (POU)-treated water and tap water[J].International Journal of Environmental Health Research,2004,14(4):253-260.
[13]	SU F Y,LUO M F,ZHANG F,et al.Performance of microbiological control by a point-of-use filter system for drinking water purification[J].Journal of Environmental Sciences,2009,21(9):1237-1246.
[14]	FARKAS A,BUTIUC-KEUL A,CIATARAS D,et al.Microbiological contamination and resistance genes in biofilms occurring during the drinking water treatment process[J].Science of the Total Environment,2013,443:932-938.
[15]	BAI X H,MA X L,XU F M,et al.The drinking water treatment process as a potential source of affecting the bacterial antibiotic resistance[J].Science of the Total Environment,2015,533:24-31.
[16]	SUHAS,GUPTA V K,CARROTT P J M,et al.Cellulose:a review as natural,modified and activated carbon adsorbent[J].Bioresource Technology,2016,216:1066-1076.
[17]	KLAPPENBACH J A,SAXMAN P R,COLE J R,et al.Rrndb:the ribosomal RNA operon copy number database[J].Nucleic Acids Research,2001,29(1):181-184.
[18]	ZHANG Y,GU A Z,CEN T Y,et al.Sub-inhibitory concentrations of heavy metals facilitate the horizontal transfer of plasmid-mediated antibiotic resistance genes in water environment[J].Environmental Pollution,2018,237:74-82.
[19]	ZHANG Y,GU A Z,HE M,et al.Subinhibitory concentrations of disinfectants promote the horizontal transfer of multidrug resistance genes within and across Genera[J].Environmental Science & Technology,2016,51(1):570-580.
[20]	SU J Q,WEI B,OU-YANG W Y,et al.Antibiotic resistome and its association with bacterial communities during sewage sludge composting[J].Environmental Science & Technology,2015,49(12):7356-7363.
[21]	JIA S Y,SHI P,HU Q,et al.Bacterial community shift drives antibiotic resistance promotion during drinking water chlorination[J].Environmental Science & Technology,2015,49(20):12271-12279.
[22]	CHEN H,ZHANG M M.Occurrence and removal of antibiotic resistance genes in municipal wastewater and rural domestic sewage treatment systems in eastern China[J].Environment International,2013,55:9-14.