FNA旁侧污泥预处理SBR-PN过程启动及N<sub>2</sub>O释放特性

张家惠; 巩有奎

doi:10.13205/j.hjgc.202106012

FNA旁侧污泥预处理SBR-PN过程启动及N₂O释放特性

doi: 10.13205/j.hjgc.202106012

烟台职业学院建筑工程系, 山东烟台 264670

基金项目:

国家自然科学基金项目（51508008）

详细信息

作者简介:
张家惠(1964-),女,学士,副教授。260943813@qq.com

计量
- 文章访问数: 87
- HTML全文浏览量: 13
- PDF下载量: 2
- 被引次数: 0
出版历程
- 收稿日期: 2020-11-15
- 网络出版日期: 2022-01-18

START-UP OF SBR-PN PROCESS AND N₂O EMISSION IN SIDE PRETREATMENT OF WASTED SLUDGE BY FREE NITROUS ACID

Department of Architecture Engineering, Yantai Vocational College, Yantai 264670, China

摘要

摘要: 在序批式生物反应器（SBR）基础上增设游离亚硝酸（FNA）污泥预处理单元，成功启动了SBR短程硝化（SBR-PN）过程。FNA对NOB的活性抑制远大于其对AOB的抑制，FNA=0.48 mg/L，两者活性差异最大。SBR运行稳定（阶段Ⅰ）后，利用0.48 mg/L的FNA对活性污泥进行24 h缺氧处理，经60 d驯化（阶段Ⅱ），SBR内NH₄⁺-N去除率和亚硝态氮积累率（NAR）均达到95%以上，总氮（TN）去除率由（22.8±3.6）%增至（35.5±3.7）%。分段进水（阶段Ⅲ）方式提高了原水有机物利用率，TN去除率达到（64.0±2.5）%，最大NO₂^-积累和N₂O产量分别由（16.4±1.6） mg/L和（0.85±0.09） mg/L降至（11.4±1.2） mg/L和（0.28±0.04） mg/L，N₂O产率由（7.40±0.99）%降至（1.33±0.26）%。基于FNA缺氧抑菌选择性差异，采用分段进水方式运行SBR，防止了高浓度NO₂^-和NH₄⁺共存，可实现稳定生活污水短程硝化过程并降低N₂O释放。经FNA处理，活性污泥蛋白质（PN）和多糖（PS）释放增加，PN/PS由阶段Ⅰ的1.42增至阶段Ⅱ、Ⅲ的1.77和1.74，SVI由阶段Ⅰ的（113±12） mL/gVSS分别增至阶段Ⅱ、Ⅲ的（129±15），（122±13） mL/gVSS。
- 游离亚硝酸 /
- 污泥处理 /
- 部分硝化 /
- 氧化亚氮 /
- 胞外聚合物
Abstract: The start up of partial nitrification by using free nitrous acid(FNA) to treat activated sludge was studied in a sequencing batch reactor(SBR) treating rural wastewater by recirculating a portion of the sludge through a side-stream sludge treatment unit. FNA is substantially more biocidal to NOB than to AOB. The difference was the highest at the FNA concentration of 0.48 mg/L. With 3.6 L activated sludge treated by 0.48 mg/L FNA for 24 h under anoxic condition each day, the stable partial nitrification in the sequencing batch reactor(SBR-PN) was established after 60 d accumulation, with the NH₄⁺ removal efficiency and the nitrite accumulation rate(NAR) were both above 95%(Stage Ⅱ). The two-step feeding strategy(Stage Ⅲ) was used to replace the one-step feeding in the SBR with the aim to reduce the NO₂^- accumulation and N₂O emission. The results showed that the maximum NO₂^- accumulation and N₂O emission were reduced from(16.4±1.6) mg/L and(0.85±0.09) mg/L to(11.4±1.2) mg/L and(0.28±0.04) mg/L, respectively, with the TN removal efficiency of(64.0±2.5)%, the N₂O yield decreased from(7.40±0.99)% to(1.33±0.26)%. Based on the selectivity of FNA on AOB and NOB, the coexistence of high NO₂^- and NH₄⁺ was prevented by two-step feeding strategy of the SBR, which was beneficial to the establishment of partial nitrification and the reduction of N₂O emission. PN/PS value increased from 1.42 in stage Ⅰ to 1.77 and 1.74 in stage Ⅱ and Ⅲ, additionally, SVI increased from(113±12) mL/gVSS to(129±15) mL/gVSS in stage Ⅱ and(122±13) mL/gVSS in stage Ⅲ respectively.
- free nitrous acid /
- sludge treatment /
- partial nitrification /
- nitrous oxide /
- EPS

HTML全文

参考文献(25)

[1]	SUSANNE L,EVA M G,SIEGFRIED E V,et al.Full-scale partial nitritation/anammox experiences an application survey[J].Water Research,2014,55:292-303.
[2]	KARTAL B,MAALCKE W J,DE A N M,et al.Molecular mechanism of anaerobic ammonium oxidation[J].Nature,2011,479(7371):127-130.
[3]	KARTAL B,KUENEN J G,LOOSDRECHT C M V M.Sewage treatment with anammox[J].Science,2010,328(5979):702-703.
[4]	HAYDÉE DE C,YAN X G,WILLY V,et al.Oland is feasible to treat sewage-like nitrogen concentrations at low hydraulic residence times[J].Appl Microbiol Biotechnol,2011,90(4):1537-1545.
[5]	PENG Y Z,ZHU G B.Biological nitrogen removal with nitrification and denitrification via nitrite pathway[J].Applied Microbiology and Biotechnology,2006,73(1):15-26.
[6]	WANG Q L,YE L,JIANG G M,et al.Side-stream sludge treatment using free nitrous acid selectively eliminates nitrite oxidizing bacteria and achieves the nitrite pathway[J].Water Research,2014,55:245-255.
[7]	MARI K W,LEVI S.New directions in biological nitrogen removal and recovery from wastewater[J].Curr Opin Biotechnol,2019,57:50-55.
[8]	VADIVELU V M,YUAN Z G,FUX C,et al.The inhibitory effects of free nitrous acid on the energy generation and growth processes of an enriched nitrobacter culture[J].Environmental Science & Technology,2006,40(14):4442-4448.
[9]	张宇坤,王淑莹,董怡君,等.游离氨和游离亚硝酸对亚硝态氮氧化菌活性的影响[J].中国环境科学,2014,34(5):1242-1247.
[10]	YAN Z,ADRIAN O,MELVIN L,et al.The role of nitrite and free nitrous acid (fna) in wastewater treatment plants[J].Water Research,2011,45(15):4672-4682.
[11]	RAVISHANKARA A R,DANIEL S J,PORTMANN W R.Nitrous oxide (N₂O):the dominant ozone-depleting substance emitted in the 21st century[J].Science,2009,326(5949):123-125.
[12]	APHA(American Public Health Association).Standard methods for the examination of water and wastewater[J].Baltimore:Port City Press,1998.
[13]	APHA(American Public Health Association).Standard methods for the examination of water and wastewater[M].14th ed.Washington DC:APHA American Public Health Association,1976.
[14]	DUBOIS M,GILLES K A,HAMILTON J K,et al.Colorimetric method for determination of sugars and related substances[J].Analytical Chemistry,1956,28(3):350-356.
[15]	BRADFORD M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry,1976,72(1/2):248-254.
[16]	YANG Q,LIU X H,PENG C Y,et al.N₂O production during nitrogen removal via nitrite from domestic wastewater:main sources and control method[J].Environmental Science & Technology,2009,43(24):9400-9406.
[17]	马琳娜,刘文龙,张琼,等.游离亚硝酸(FNA)对A²O污泥菌群结构的影响[J].中国环境科学,2017,37(7):2566-2573.
[18]	王增花,王博,宫小斐,等.FNA处理絮体污泥恢复城市污水PN/A工艺短程硝化[J].中国环境科学,2019,39(8):3308-3315.
[19]	MARLIES J K,HARDY T,ROBBERT K,et al.Nitrous oxide emission during wastewater treatment[J].Water Research,2009,43(17):4093-4103.
[20]	巩有奎,彭澄瑶,王淑莹,等.流量分配对短程生物脱氮过程中N₂O产生的影响[J].环境科学学报,2014,34(7):1668-1673.
[21]	GONG Y K,PENG Y Z,YANG Q,et al.Formation of nitrous oxide in a gradient of oxygenation and nitrogen loading rate during denitrification of nitrite and nitrate[J].Journal of Hazardous Materials,2012:453-460.
[22]	WANG Q L,YE L,JIANG G M,et al.Free nitrous acid (fna)-based pretreatment enhances methane production from waste activated sludge[J].Environmental Science & Technology,2013,47(20):11897-11904.
[23]	李冬,曹美忠,郭跃洲,等.进水氨氮浓度对生物除磷颗粒系统的影响[J].环境科学,2019,40(3):1360-1366.
[24]	高春娣,赵楠,安冉,等.FNA对短程硝化污泥菌群结构的影响[J].中国环境科学,2019,39(5):1977-1984.
[25]	WU J W,YANG Q,LUO W,et al.Role of free nitrous acid in the pretreatment of waste activated sludge:extracellular polymeric substances disruption or cells lysis?[J].Chemical Engineering Journal,2018,336:28-37.