RESEARCH PROGRESS ON METHANOGENIC INHIBITION TECHNOLOGY DURING ANAEROBIC DIGESTION OF EXCESS SLUDGE
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摘要: 剩余污泥厌氧发酵过程中产生的挥发性脂肪酸(VFA)相比甲烷具有更高的应用价值,因而受到广泛关注。研究发现,通过产甲烷抑制剂可以将厌氧发酵控制在产酸阶段,阻断产甲烷过程,从而实现VFA的大量积累。然而,目前产甲烷抑制剂存在分类不明确、部分产甲烷抑制剂机理研究不够完善等问题。因此,根据抑制产甲烷菌物质的来源和特性,将剩余污泥厌氧消化过程中产甲烷抑制剂分为内源抑制、外源抑制和生物抑制3类,分别阐述了其抑制机理及其对厌氧发酵产酸过程的影响,分析了各种抑制剂的研究现状及不足之处,指出抑制剂的毒性抑制研究和水解机理研究将是今后的研究重点,同时应进一步研究了厌氧发酵系统中微生物之间的竞争关系,明确微生物在剩余污泥厌氧发酵产酸和抑制产甲烷过程中的作用机制。Abstract: The volatile fatty acid(VFA) produced in the anaerobic fermentation of excess sludge has higher application value than methane, and thus has attracted extensive attention. It was found that the anaerobic fermentation could be controlled in the acid production stage by methanogenic inhibitors, and the methanogenic process could be blocked, so as to achieve a large amount of accumulation of VFA. However, the classification of methanogenic inhibitors is not clear and the mechanism of some methanogenic inhibitors is not perfect. Therefore, according to the sources and characteristics of the substance inhibiting methanogens, methane inhibitors were divided into three categories:endogenous inhibition, exogenous inhibition and biological inhibition. We expounded several inhibitors of inhibition mechanism and the influence of the process of anaerobic fermentation to produce acid, analyzed present research situation and deficiencies of various inhibitors, pointed out that the toxicity of inhibitors inhibit and hydrolysis mechanism research was the research emphasis in the future. The competitive relationship between microorganisms in anaerobic fermentation system should be further studied, the mechanism of microorganisms in the process of acid production, and methane production inhibition by anaerobic fermentation of residual sludge should also be clarified.
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[1] LEE W S,CHUA A S M,YEOH H K,et al.A review of the production and applications of waste-derived volatile fatty acids[J].Chemical Engineering Journal,2014,235:83-99. [2] KLEEREBEZEM R,VAN LOOSDRECHT M C.Mixed culture biotechnology for bioenergy production[J].Current Opinion in Biotechnology,2007,18(3):207-212. [3] UYAR B,EROGLU I,YUCEL M,et al.Photofermentative hydrogen production from volatile fatty acids present in dark fermentation effluents[J].International Journal of Hydrogen Energy,2009,34(10):4517-4523. [4] LI X,CHEN H,HU L F,et al.Pilot-scale waste activated sludge alkaline fermentation,fermentation liquid separation,and application of fermentation liquid to improve biological nutrient removal[J].Environmental Science & Technology,2011,45(5):1834-1839. [5] MA H J,CHEN X C,LIU H,et al.Improved volatile fatty acids anaerobic production from waste activated sludge by pH regulation:alkaline or neutral pH?[J].Waste Management,2016,48:397-403. [6] JIANG S,CHEN Y G,ZHOU Q.Effect of sodium dodecyl sulfate on waste activated sludge hydrolysis and acidification[J].Chemical Engineering Journal,2007,132:311-317. [7] YU S Y,ZHANG G M,LI J Z,et al.Effect of endogenous hydrolytic enzymes pretreatment on the anaerobic digestion of sludge[J].Bioresource Technology,2013,146:758-761. [8] GONZALEZA,HENDRIKS A,VAN LIER J B,et al.Pretreatments to enhance the biodegradability of waste activated sludge:elucidating the rate limiting step[J].Biotechnology Advances,2018,36(5):1434-1469. [9] 郝晓地,唐兴,曹亚莉.腐殖质对污泥厌氧消化的影响及其屏蔽方法[J].环境科学学报,2017,37(2):407-418. [10] CERVANTES F J,VAN DER VELDE S,LETTINGA G,et al.Competition between methanogenesis and quinone respiration for ecologicall important substrates in anaerobic consortia[J].Fems Microbiolog Ecology,2000,34(2):161-171. [11] DOS SANTOS A B,CERVANTES F J,VAN LIER J B.Azo dye reduction b thermophilic anaerobic granular sludge,and the impact of the redo mediator anthraquinone-2,6-disulfonate(AQDS)on the reductive biochemical transformation[J].Applied Microbiology and Biotechnology,2004,64(1):62-69. [12] LI H,LI Y K,JIN Y Y,et al.Recovery of sludge humic acids with alkaline pretreatment and its impact on subsequent anaerobic digestion[J].Journal of Chemical Technology & Biotechnology,2014,89(5):707-713. [13] 郝晓地,唐兴,李季,等.腐殖酸影响剩余污泥厌氧消化过程实验研究[J].环境科学学报,2018,38(8):3061-3068. [14] LIU K,CHEN Y G,XIAO N D,et al.Effect of humic acids with different characteristics on fermentative short-chain fatty acids production from waste activated sludge[J].Environmental Science &Technology,2015,49(8):4929-4936. [15] CERVANTES F J,DE BOK F A M,DUONG-DAC T,et al.Reduction on humic substances by halorespiring,sulphate-reducing and methanogenic microorganisms[J].Environmental Microbiology,2002,4(1):51-57. [16] DOS SANTOS A B,CERVANTES F J,VAN LIER J B.Azo dye reduction b thermophilic anaerobic granular sludge,and the impact of the redo mediator anthraquinone-2,6-disulfonate(AQDS)on the reductiv biochemical transformation[J].Applied Microbiology and Biotechnology,2004,64(1):62-69. [17] 马晨,周顺桂,庄莉,等.微生物胞外呼吸电子传递机制研究进展[J].生态学报,2011,31(7):2008-2018. [18] FERNANDES T V,LIER J B V,ZEEMAN G.Humic acid-like and fulvi acid-like inhibition on the hydrolysis of cellulose and tributyrin[J].Bioenergy Research,2014,8(2):821-831. [19] LUO K,YANG Q,LI X M,et al.Novel insights into enzymatic-enhanced anaerobic digestion of waste activated sludge by three-dimensional excitation and emission matrix fluorescence spectroscopy[J].Chemosphere,2013,91(5):579-585. [20] AZMAN S,KHADEM A,ZEEMAN G,et al.Mitigation of humic acid inhibition in anaerobic digestion of cellulose by addition of various salts[J].Bioengineering,2015,2(2):54-65. [21] JIN B D,WANG S Y,XING L Q,et al.Effect of salinity on enhancing waste activated sludge alkaline fermentation at different temperatures[J].CLEAN-Soil Air Water,2016,44(12):1750-1758. [22] ZHAO J W,LIU Y W,WANG D B,et al.Potential impact of salinity on methane production from food waste anaerobic digestion[J].Waste Management,2017,67:308-314. [23] ZHAO J W,WANG D B,LIU Y W,et al.Novel stepwise pH control strategy to improve short chain fatty acid production from sludge anaerobic fermentation[J].Bioresource Technology,2018,249:431-438. [24] ZHAO J W,ZHANG C,WANG D B,et al.Revealing the underlying mechanisms of how sodium chloride affects short-chain fatty acid production from the cofermentation of waste activated sludge and food waste[J].ACS Sustainable Chemistry & Engineering,2016,4(9):4675-4684. [25] 夏旦凌,金潇,徐期勇.餐厨垃圾高温产甲烷的高盐度抑制及恢复性研究[J].环境工程学报,2016,10(3):1426-1431. [26] SU G Q,WANG S Y,YUAN Z G,et al.Enhanced volatile fatty acids production of waste activated sludge under salinity conditions:performance and mechanisms[J].Journal of Bioscience and Bioengineering,2016,121(3):293-298. [27] CUI Y W,SU H,CHEN Y F,et al.Mechanism of activated sludge floc disintegration induced by excess addition of NaCl[J].CLEAN-Soil,Air,Water,2015,43(8):1197-1206. [28] WESTON N B,VILE M A,NEUBAUER S C,et al.Acceleratedmicrobial organic matter mineralization following salt-waterintrusion into tidal freshwater marsh soils[J].Biogeochemistry,2011,102:135-151. [29] POFFENBARGER H J,NEEDELMAN B A,MEGONIGAL J P.Salinityinfluence on methane emissions from tidal marshes[J].Wetlands,2011,31:831-842. [30] RINZEMA A,VAN LIER J,LETTINGA G.Sodiuminhibition of ace-toclastic methanogens in granular sludge from a UASB reactor[J].Enzyme and Microbial Technology,1988,10(1):24-32. [31] YANG Z Y,YANG W,HE Y F,et al.Effect of ammonia on methane production,methanogenesis pathway,microbial community and reactor performance under mesophilic and thermophilic conditions[J].Renewable Energy,2018,125:915-925. [32] KAYHANIAN M.Performance of a high-solids anaerobic digestion process under various ammonia concentrations[J].Journal of Chemical Technology and Biotechnology,1994,59(4):349-352. [33] 何仕均,王建龙,赵璇.氨氮对厌氧颗粒污泥产甲烷活性的影响[J].清华大学学报(自然科学版),2005,9(45):1294-1296. [34] 张寓涵,潘云霞,贺亚清,等.不同氮源对猪粪废水厌氧发酵氨抑制效果的影响[J].环境工程学报,2020,14(4):955-962. [35] ASTALS S,PECES M,BATSTONE D J,et al.Characterising and modelling free ammonia and ammonium inhibition in anaerobic systems[J].Water Research,2018,143:127-135. [36] 王晋,李习伟,方新磊,等.基于氨氮抑制效应的污泥厌氧产酸[J].环境工程学报,2017,11(5):3091-3098. [37] NOLLET L,DEMEYER D,VERSTRAETE W.Effect of 2-bromoethanesulfonic acid and Peptostreptoco-ccus productus ATCC 35244 addition on stimulation of reductive acetogenesis in the ruminal ecosystem by selective inhibition of methanogenesis[J].Applied and Environmental Microbiology,1997,63:194-200. [38] KIM J R,MIN B,LOGAN B E.Evaluation of procedures to acclimate a microbial fuel cell for electricity production[J].Applied Microbiology & Biotechnology,2005,68:23-30. [39] CHIDTHAISONG A,CONRAD R.Specificity of chloroform,2-bromoethane sulfonate and fluoroacetate to inhibit methanogenesis and other anaerobic processes in anoxic rice field soil[J].Soil Biology and Biochemistry,2000,32(7):977-988. [40] 刘和,许科伟,王晋,等.污泥厌氧消化产酸发酵过程中乙酸累积机制[J].微生物学报,2010,50(10):1327-1333. [41] 赵晓艳,董滨,戴晓虎.污泥生化产酸潜势与生化产甲烷潜势的相关性[J].环境科学与技术,2016,39(7):86-90,95. [42] 陈思远,陈刚,高彩琪,等.BES对污泥厌氧发酵产酸性能的影响[J].江西理工大学学报,2020,41(1):50-56. [43] SARAVANAN R SHANMUGAM,SUBBA RAO CHAGANTI,JERALD A.Lalman,et al.Effect of inhibitors on hydrogen consumption and microbial population dynamics in mixed anaerobic cultures[J].International Journal of Hydrogen Energy,2014,39(1):249-257. [44] LI J,WANG T,SHAO B,et al.Plasmid-mediated quinolone resistance genes and antibiotic residues in wastewater and soil adjacent to swine feedlots:potential transfer to agricultural lands[J].Environment Health Perspect,2010,120(8):1144-1149. [45] ZHAI W C,YANG F X,MAO D Q,et al.Fate and removal of various antibiotic resistance genes in typical pharmaceutical wastewater treatment systems[J].Environmental Science and Pollution Research,2016,23(12):12030-12038. [46] CETECIOGLU Z,ORHON D.How do sulfamethoxazole and tetracycline affect the utilization of short chain fatty acids under anaerobic conditions[J].Journal of Environmental Chemical Engineering,2018,6:1305-1313. [47] NI B J,ZENG S T,WEI W,et al.Impact of roxithromycin on waste activated sludge anaerobic digestion:methane production,carbon transformation and antibiotic resistance genes[J].Science of the Total Environment,2020,703:134899. [48] LU X Q,ZHEN G Y,LIU Y,et al.Long-term effect of the antibiotic cefalexin on methane production during waste activated sludge anaerobic digestion[J].Bioresource Technology,2014,169:644-651. [49] HUANG X D,XU Q X,WU Y X,et al.Effect of clarithromycin on the production of volatile fatty acids from waste activated sludge anaerobic fermentation[J].Bioresource Technology,2019,288:121598. [50] CARLOS A.RAMÍREZ-RESTREPO,CUI TAN,CHRISTOPHER J.O'NEILL,et al.Methane production,fermentation characteristics,and microbial profiles in the rumen of tropical cattle fed tea seed saponin supplementation[J].Animal Feed Science and Technology,2016,216:58-67. [51] H Y TAN,C C SIEO,N ABDULLAH,et al.Effects of condensed tannins from Leucaenaon methane production,rumen fermentation and populations of methanogens and protozoa in vitro[J].Animal Feed Science and Technology,2011,3/4(169):185-193. [52] SERRANO A,ÁNGEL SILES LÓPEZ J,CHICA A F,et al.Mesophilic anaerobic co-digestion of sewage sludge and orange peel waste[J].Environmental Technology,2014,35(7):898-906. [53] A CARVALHO,R FRAGOSO,J GOMINHO,et al.Effect of minimizing d-limonene compound on anaerobic Co-digestion feeding mixtures to improve methane yield[J].Waste and Biomass Valorization volume,2017,2019(10):75-83. [54] 冯俊丽,马鲁铭.高浓度硫酸盐废水的厌氧生物处理[J].环境保护科学,2005,31(127):23-26. [55] ZHANG L T,HUANG X F,XUE B,et al.Immunization against rumen methanogenesis by vaccination with a new recombinant protein[J].PloS One,2015,10(10):eθ140086. [56] 夏涛.硫酸盐还原菌对厌氧消化促进作用的研究[D].南京:南京农业大学,2008. [57] 樊雅欣.过硫酸盐预处理协同硫酸盐还原菌强化剩余污泥厌氧发酵产酸研究[D].太原:太原理工大学,2019. [58] PAUL S S,DEB S M,SINGH D.Isolation and characterization of novel sulphate-reducing Fusobacterium sp.and their effects on in vitro methane emission and digestion of wheat straw by rumen fluid from Indian riverine buffaloes[J].Animal Feed Science and Technology,2011,166/167:132-140. [59] ANDERSON R C,RASMUSSEN M A.Use of a novel nitrotoxin-metabolizing bacterium to reduce ruminal methane production[J].Bioresource Technology,1998,64(2):89-95. [60] BAYAT A R,KAIRENIUS P,STEFANSKI T,et al.Effect of camelina oil or live yeasts(Saccharomyces cerevisiae)on ruminal methane production,rumen fermentation,and milk fatty acid composition in lactating cows fed grass silage diets[J].Journal of Dairy Science,2015,98(5):3166-3181. [61] WOODA T A,WALLACE R J,ROWE A,et al.Encapsulated fumaric acid as a feed ingredient to decrease ruminal methane emissions[J].Animal Feed Science and Technology,2009,152(1/2):62-71.
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