INFLUENCE OF SLUDGE INOCULATION VOLUME ON METHANOGENESIS OF RESIDUE FROM ANAEROBIC FERMENTATIVE HYDROGEN PRODUCTION USING COMBINED SLUDGE AND FOOD WASTE UNDER MEDIUM TEMPERATURE CONDITION
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摘要: 污泥与餐厨垃圾联合厌氧发酵产氢余物产甲烷可有效发挥产氢余物的资源化潜力。研究了中温条件下不同污泥接种量对污泥与餐厨垃圾联合厌氧发酵产氢余物的影响,分析其产气产甲烷能力及反应前后体系糖类、蛋白质、挥发性脂肪酸(TVFA)、pH及氨氮(NH3-N)的变化情况,以寻求最佳接种量与相应体系指标变化规律。结果表明:中温条件下,过低或过高的接种量下产氢余物产甲烷效果均不佳。30%接种量的体系产甲烷能力最优,甲烷百分比增速最快,并有最大累积产甲烷量171.1 mL/gDS;有机物均得到了明显的消耗,总糖降解了39.01%,总蛋白质降解了28.09%,其中糖类物质降解以可溶糖为主,不溶蛋白质与可溶蛋白质的降解量相当,反应后体系pH升高。Abstract: Methane production from residue of anaerobic fermentative hydrogen production using combined sludge and food waste is a promising method to further exploit the potential resource of the residue of hydrogen production. This study explored the influence of inoculation volume on the methane production from the residue of anaerobic hydrogen fermentation using combined sludge and food waste under medium temperature condition, analyzed its gas and methane production performance and the changes of the substrate in the anaerobic system before and after fermentation, aimed to seek the optimum inoculation volume and the corresponding changes of the substrate in the anaerobic system, including saccharide, protein, TVFA, pH and ammonia. The results showed that, under medium temperature conditions, neither high nor low inoculation volume was conducive to methane production performance improvement. The optimum inoculation volume was 30%, then we got the fastest growth rate of methane concentration during the anaerobic fermentation, and the largest accumulative methane yield was 171.1 mL/gDS. The changes of substrate concentration in the anaerobic system proved that organic substrates were consumed greatly during fermentation, and the degradation yield of total saccharide was 39.01% and the total protein was 28.09%. It was also found that degradation rate of soluble saccharide was greater than insoluble saccharide, and the former mainly contributed to the saccharide degradation; while the degradation of insoluble and soluble amount of protein was equivalent. After the fermentation was finished, the pH in the system rose.
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