两级动态膜反应器脱氮性能及微生物相互作用

罗晓楠; 杨一清; 张楠; 孟凡刚

doi:10.13205/j.hjgc.202107013

两级动态膜反应器脱氮性能及微生物相互作用

doi: 10.13205/j.hjgc.202107013

罗晓楠^1,2,3,
杨一清⁴,
张楠⁴,
孟凡刚^1,3, ,

1. 中山大学环境科学与工程学院, 广州 510006;
2. 广东工业大学环境生态工程研究院, 广州 510006;
3. 广东省环境污染控制与修复技术重点实验室, 广州 510006;
4. 广州环投环境服务有限公司, 广州 514000

基金项目:

广东省科技创新战略专项（2018B020202）；山东省重大科技创新工程项（2019JZZY020308）

详细信息

作者简介:
罗晓楠(1990-),男,博士,主要研究方向为废水生物脱氮。xiaonanluo_gd@163.com

通讯作者:
孟凡刚(1980-),男,博士,教授,主要研究方向为废水处理与资源化。mengfg@mail.sysu.edu.cn

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出版历程
- 收稿日期: 2021-01-20
- 网络出版日期: 2022-01-18

PERFORMANCE OF NITROGEN REMOVAL AND MICROBIAL INTERACTION IN A TWO-STAGE DYNAMIC MEMBRANE BIOREACTOR

LUO Xiao-nan^1,2,3,
YANG Yi-qing⁴,
ZHANG Nan⁴,
MENG Fan-gang^{1,3
, ,}

1. School of Environmental Science and Engineering, Sun Yat-sen University, Guangzhou 510006, China;
2. Institute of Environmental & Ecological Engineering, Guangdong University of Technology, Guangzhou 510006, China;
3. Guangdong Provincial Key Laboratory of Environmental Pollution Control and Remediation Technology, Guangzhou 510006, China;
4. Grandtop Environmental Service Co., Ltd, Guangzhou 514000, China

摘要

摘要: 动态膜对跨膜压差要求较低，在保证悬浮物去除效果同时对减少能耗有关键作用。同时，动态膜也可以保护用于低C/N污水脱氮的厌氧氨氧化菌（AMX）。在设置动态膜的A/O工艺中处理低C/N废水，将异养菌和自养菌在A和O池中进行分离，并在O池絮状污泥中富集好氧氨氧化菌（AOB），生物膜上富集厌氧氨氧化菌（AMX）。反应器在C/N=1情况下，NH₃-N、TN和COD去除率分别为（91.2±7.5）%、（81.0±8.1）%和（86.4±7.5）%。COD主要在A池去除，TN主要在O池去除。重力驱动出水的动态膜运行效果良好，稳定运行下出水ρ（SS）为（12.2±4.5） mg/L。系统利用动态膜和生物膜实现了功能菌之间的空间分离，反硝化菌主要分布在A池（相对丰度约30%），AOB主要分布在O池中悬浮污泥（相对丰度约5%），而AMX富集在O池生物膜上（相对丰度>20%）。生态网络分析显示，微生物群落中的正相关关系比例均超过70%，保证了系统的良好性能。可见，PN/A、动态膜及A/O结合工艺可提高不同功能微生物协同作用，在低C/N污水脱氮方面有较好效果，具有良好应用前景。
- 自养脱氮 /
- 动态膜 /
- 反硝化 /
- 微群落结构 /
- 微生物相互作用
Abstract: Dynamic membrane(DM) requires a low transmembrane pressure, which plays a key role in suspended solid removal and energy saving during wastewater treatment. DM can also protect anaerobic ammonium-oxidizing bacteria(AMX) which is used to increase nitrogen removal efficiency in wastewater with low C/N ratio. DM separated heterotrophs and autotrophs in A/O reactor treating low C/N wastewater into anaerobic tank and aerobic tank, respectively. Ammonium-oxidizing bacteria(AOB) was enriched in suspended sludge, and anaerobic ammonium-oxidizing bacteria(AMX) was enriched in biofilms of the O tank. Removal efficiency of ammonium, total nitrogen, and COD were(91.2±7.5)%,(81.0±8.1)%, and(86.4±7.5)%, respectively, with an influent C/N value of 1.0. COD was mainly removed in the A tank and total nitrogen in the O tank. Gravity-driven dynamic membranes achieved satisfactory suspended solid removal with a concentration of(12.2±4.5) mg/L in the effluent. Functional bacteria were enriched in different parts of the reactor by using dynamic membranes and biofilms. Denitrifiers were mainly present in the anaerobic tank(relative abundance of ca. 30%). The AOB was mainly enrich in suspended sludge in aerobic tank sludge, with relative abundance of ca. 5%, and anammox bacteria were abundant in the biofilm of the O tank.Resultsfrom network analysis showed that positive interactions accounted for more than 70% of all interactions among microbes in each community, which ensured the performance of the reactor. In summary, the combination of PN/A, DM, and A/O process, which improved the synergy of different functional bacteria and ensured nitrogen removal in wastewater with low C/N ratio, was a promising method for wastewater treatment.
- autotrophic nitrogen removal /
- dynamic membranes /
- denitrification /
- community structure /
- microbial interaction

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