STABLE OPERATION AND IMPACT RESISTANCE OF CANON PROCESS BASED ON PURE MBBR
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摘要: 应用CANON-MBBR系统处理实际污泥厌氧消化脱水液,考察了系统的稳定运行控制策略。结果表明:稳定期间,系统出水ρ(NH4+-N)稳定低于25 mg/L,去除率>96%;出水ρ(TIN)<70 mg/L,去除率>87%。系统维持纯生物膜运行,无须进行污泥回流与菌种的补投,实现了稳定的自养脱氮过程。运行期间,考察了水量、DO及温度对系统稳定性的影响,结果表明:在进水流量为80 m3/d(为平稳期1.33倍)、ρ(DO)高达2~4 mg/L和24 ℃低温条件下系统依然保持较高的脱氮性能。高通量测序结果表明,系统优势氨氧化菌(AOB)和厌氧氨氧化菌(AnAOB)分别为Nitrosomonas和Candidatus Kuenenia,其相对丰度均值分别为6.5%和30.2%,亚硝酸盐氧化菌(NOB)的相对丰度始终低于0.1%,NOB被成功抑制。悬浮载体生物膜实现了AOB和AnAOB的高效富集,系统较低的AOB丰度限制了系统脱氮能力的进一步提升。Abstract: A CANON-MBBR system was used to treat the actual sludge-digestion wastewater, and stable operation control strategy of the system was investigated. The results showed that during the stable period, the ammonia nitrogen concentration in the effluent of the system was stable below 25 mg/L and the removal rate was above 96%; the TIN concentration in the effluent was below 70 mg/L and the removal rate was above 87%. The system maintained pure MBBR without the sludge backflow and supplemental injection of strains, and achieved a stable autotrophic denitrification process. During the operation, the impact of water quantity, DO and temperature on the stability of the system were investigated. The results showed that the system still maintained high nitrogen removal performance under the conditions of 1.33 times of water impact, high DO range of 2~4 mg/L and low temperature of 24 ℃. The ammonia-oxidizing bacteria (AOB) and anaerobic ammonia-oxidizing bacteria (AnAOB) were Nitrosomonas and Candidatus Kuenenia, respectively, and their average relative abundance were 6.5% and 30.2%, respectively. The relative abundance of nitrite oxidizing bacteria (NOB) was always lower than 0.1%, which realized NOB suppression. AOB and AnAOB were enriched efficiently by suspended carrier biofilm, but the low AOB abundance of the system limited the further improvement of the system’s nitrogen removal performance.
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[1] DU R,CAO S B,WANG S Y,et al.Performance of partial denitrification (PD)-ANAMMOX process in simultaneously treating nitrate and low C/N domestic wastewater at low temperature[J].Bioresource Technologyogy,2016,219:420-429. [2] MIAO L,WANG S Y,CAO T H,et al.Advanced nitrogen removal from landfill leachate via Anammox system based on Sequencing Biofilm Batch Reactor (SBBR):effective protection of biofilm[J].Bioresource Technology,2016,220:8-16. [3] ZHANG L,ZHANG S J,PENG Y Z,et al.Nitrogen removal performance and microbial distribution in pilot- and full-scale integrated fixed-biofilm activated sludge reactors based on nitritation-anammox process[J].Bioresource Technology,2015,196:448-453. [4] THIRD K A,SLIEKERS A O,KUENEN J G,et al.The CANON System (Completely Autotrophic Nitrogen-removal Over Nitrite) under Ammonium Limitation:interaction and Competition between Three Groups of Bacteria[J].Systematic and Applied Microbiology,2001,24(4):588-596. [5] VAN DER STAR W R L,ABMA W R,BLOMMERS D,et al.Startup of reactors for anoxic ammonium oxidation:experiences from the first full-scale anammox reactor in Rotterdam[J].Water Research,2007,41(18):4149-4163. [6] THIRD K A,SLIEKERS A O,KUENEN J G,et al.The CANON system (completely autotrophic nitrogen-removal over nitrite) under ammonium limitation:interaction and competition between three groups of bacteria[J].Systematic and Applied Microbiology,2001,24(4):588-596. [7] WANG W G,WANG Y Y,WANG X D,et al.Dissolved oxygen microelectrode measurements to develop a more sophisticated intermittent aeration regime control strategy for biofilm-based CANON systems[J].Chemical Engineering Journal,2019,365:165-174. [8] 孙庆花,吴迪,周家中,等.CANON中试反应器启动及性能优化[J].环境科学,2019,40(7):3169-3178. [9] ZHENG B Y,ZHANG L,GUO J H,et al.Suspended sludge and biofilm shaped different anammox communities in two pilot-scale one-stage anammox reactors[J].Bioresource Technology,2016,211:273-279. [10] LI X,LU M Y,QIU Q C,et al.The effect of different denitrification and partial nitrification-Anammox coupling forms on nitrogen removal from mature landfill leachate at the pilot-scale[J].Bioresource Technology,2020,295:111430. [11] XU X C,WANG G,ZHOU L,et al.Start-up of a full-scale SNAD-MBBR process for treating sludge digester liquor[J].Chemical Engineering Journal,2018,343:477-483. [12] 国家环境保护局.水和废水监测分析方法[M].北京:环境科学出版社,2002. [13] 李继宏,单士亮,李亮,等.膜生物反应器中EPS的提取方法[J].环境工程,2013,31(3):10-14. [14] XU N,TAN G C,WANG H Y,et al.Effect of biochar additions to soil on nitrogen leaching,microbial biomass and bacterial community structure[J].European Journal of Soil Biology,2016,74:1-8. [15] WANG Z Z,LIANG H,QU F S,et al.Start up of a gravity flow CANON-like MBR treating surface water under low temperature[J].Chemical Engineering Journal,2013,217:466-474. [16] 管勇杰,吴迪,周家中,等.CANON-MBBRTM自养脱氮工艺接种启动及稳定运行控制[J].中国给水排水,2019,35(7):27-32. [17] CHRISTENSSON M,EKSTRÖM S,CHAN A A,et al.Experience from start-ups of the first ANITA Mox Plants[J].Water Science and Technology,2013,67(12):2677-2684. [18] HAO X,HEIJNEN J J,VAN LOOSDRECHT M C M.Model-based evaluation of temperature and inflow variations on a partial nitrification-ANAMMOX biofilm process[J].Water Research,2002,36(19):4839-4849. [19] THIRD K A,PAXMAN J,SCHMID M,et al.Treatment of nitrogen-rich wastewater using partial nitrification and anammox in the CANON process[J].Water Science and Technology,2005,52(4):47-54. [20] WETT B.Development and implementation of a robust deammonification process[J].Water Science and Technology:a Journal of the International Association on Water Pollution Research,2007,56(7):81-88. [21] 赵婉情,李柏林,王伟,等.颗粒-絮状污泥耦合单级自养脱氮系统的脱氮性能分析[J].环境工程,2020,38(9):43-47,199. [22] ZHANG X J,ZHANG N,WANG L N,et al.Bioactivity and microbial community structure of nitrite-oxidizing bacteria in five membrane bioreactors operated as CANON process with different C/N ratio[J].Ecological Engineering,2017,99:159-163. [23] WANG W G,WANG Y Y,WANG X D,et al.Dissolved oxygen microelectrode measurements to develop a more sophisticated intermittent aeration regime control strategy for biofilm-based CANON systems[J].Chemical Engineering Journal,2019,365:165-174. [24] HUYNH T V,NGUYEN P D,PHAN T N,et al.Application of CANON process for nitrogen removal from anaerobically pretreated husbandry wastewater[J].International Biodeterioration & Biodegradation,2019,136:15-23. [25] BÉLINE F,BOURSIER H,DAUMER M L,et al.Modelling of biological processes during aerobic treatment of piggery wastewater aiming at process optimisation[J].Bioresource Technology,2007,98(17):3298-3308. [26] ZHANG X J,LI D,LIANG Y H,et al.Autotrophic nitrogen removal from domestic sewage in MBR-CANON system and the biodiversity of functional microbes[J].Bioresource Technology,2013,150:113-120. [27] CHO S,FUJII N,LEE T,et al.Development of a simultaneous partial nitrification and anaerobic ammonia oxidation process in a single reactor[J].Bioresource Technology,2011,102(2):652-659. [28] GUO J S,YANG G H,FANG F,et al.Performance of completely autotrophic nitrogen removal over nitrite process under different aeration modes and dissolved oxygen[J].Frontiers of Environmental Science & Engineering in China,2008,2(4):439-445. [29] QIAO S,NISHIYAMA T,FUJII T,et al.Rapid startup and high rate nitrogen removal from anaerobic sludge digester liquor using a SNAP process[J].Biodegradation,2012,23(1):157-164. [30] YUE X,YU G P,LU Y Q,et al.Effect of dissolved oxygen on nitrogen removal and the microbial community of the completely autotrophic nitrogen removal over nitrite process in a submerged aerated biological filter[J].Bioresource Technology,2018,254:67-74. [31] STROUS M,KUENEN J G,JETTEN M S M.Key Physiology of Anaerobic Ammonium Oxidation[J].Applied and Environmental Microbiology,1999,65(7):3248-50. [32] LIU T,LI D,ZHANG J,et al.Effect of temperature on functional bacterial abundance and community structure in CANON process[J].Biochemical Engineering Journal,2016,105:306-313. [33] 王振,朱振华,丁亚男,等.低温对CANON型序批式生物膜反应器脱氮的影响[J].中国环境科学,2019,39(4):1533-1541. [34] 宋成康,王亚宜,韩海成,等.温度降低对厌氧氨氧化脱氮效能及污泥胞外聚合物的影响[J].中国环境科学,2016,36(7):2006-2013. [35] WILÉN B,LUMLEY D,MATTSSON A,et al.Relationship between floc composition and flocculation and settling properties studied at a full scale activated sludge plant[J].Water Research,2008,42(16):4404-4418. [36] PAREDES D,KUSCHK P,MBWETTE T S A,et al.New aspects of microbial nitrogen transformations in the context of wastewater treatment:a review[J].Engineering in Life Sciences,2007,7(1):13-25. [37] HANS-CURT F,JOST W.The biofilm matrix[J].Nature Reviews.Microbiology,2010,8(9):623-633. [38] 陈园园,彭党聪.生物膜中EPS与微生物的分布及测定[J].中国给水排水,2017,33(15):25-30. [39] SKILLMAN L C,SUTHERLAND I W,JONES M V.The role of exopolysaccharides in dual species biofilm development[J].Journal of Applied Microbiology,1998,85 Suppl 1(S1):13S. [40] WANG Y Y,CHEN J,ZHOU S,et al.16S rRNA gene high-throughput sequencing reveals shift in nitrogen conversion related microorganisms in a CANON system in response to salt stress[J].Chemical Engineering Journal,2017,317:512-521. [41] KUENEN J G.Anammox bacteria:from discovery to application[J].Nature Reviews Microbiology,2008,6(4):320-326. [42] LIANG Y H,LI D,ZHANG X J,et al.Nitrogen removal and microbial characteristics in CANON biofilters fed with different ammonia levels[J].Bioresource Technology,2014,171:168-174. [43] GONZALEZ M A,RODRIGUEZ S A,GARCIA M J,et al.Performance and bacterial community dynamics of a CANON bioreactor acclimated from high to low operational temperatures[J].Chemical Engineering Journal,2016,287:557-567. [44] PEREIRA A D,FERNANDES L D A,CASTRO H M C,et al.Nitrogen removal from food waste digestate using partial nitritation-anammox process:effect of different aeration strategies on performance and microbial community dynamics[J].Journal of Environmental Management,2019,251:109562. [45] HE S L,CHEN Y,QIN M,et al.Effects of temperature on anammox performance and community structure[J].Bioresource Technology,2018,260:186-195. [46] GARCÍA-RUIZ M J,MAZA-MÁRQUEZ P,GONZÁLEZ-LÓPEZ J,et al.Nitrogen removal capacity and bacterial community dynamics of a Canon biofilter system at different organic matter concentrations[J].Chemosphere,2018,193:591-601. [47] TERADA A,SUGAWARA S,YAMAMOTO T,et al.Physiological characteristics of predominant ammonia-oxidizing bacteria enriched from bioreactors with different influent supply regimes[J].BioChemical Engineering Journal,2013,79:153-161.
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