不同pH条件下小球藻氨氮处理及生物质生产能力

姜人源; 朱顺妮; 王忠铭; 袁振宏

doi:10.13205/j.hjgc.202109007

不同pH条件下小球藻氨氮处理及生物质生产能力

doi: 10.13205/j.hjgc.202109007

姜人源^1,2,3,4,
朱顺妮^1,2,3, ,,
王忠铭^1,2,3,
袁振宏^1,2,3

1. 中国科学院广州能源研究所, 广州 510640;
2. 中国科学院可再生能源重点实验室, 广州 510640;
3. 广东省新能源与可再生能源研究开发与应用重点实验室, 广州 510640;
4. 中国科学院大学, 北京 100049

基金项目:

广东省自然科学基金（2018A030313883）；国家自然科学基金（21878291）。

详细信息

作者简介:
姜人源(1995-),女,硕士研究生,主要研究方向为微藻污水处理研究。jiangrenyuan18@mails.ucas.ac.cn

通讯作者:
朱顺妮(1981-),女,博士,研究员,主要研究方向为生物质生化转化研究。zhusn@ms.giec.ac.cn

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出版历程
- 收稿日期: 2020-09-08
- 网络出版日期: 2022-01-21

RESEARCH ON CHLORELLA'S AMMONIA NITROGEN TREATMENT ABILITY AND BIOMASS PRODUCTION UNDER DIFFERENT pH CONDITIONS

JIANG Ren-yuan^1,2,3,4,
ZHU Shun-ni^{1,2,3
, ,},
WANG Zhong-ming^1,2,3,
YUAN Zhen-hong^1,2,3

1. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou 510640, China;
2. CAS Key Laboratory of Renewable Energy, Guangzhou 510640, China;
3. Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, China;
4. University of Chinese Academy of Sciences, Beijing 100049, China

摘要

摘要: 针对目前沼液中高浓度氮氨氮对空气和水质的不良影响，探究在不同pH条件下小球藻对氨氮的处理能力以及生物质生产潜力。在初始ρ（NH₃-H）为120~130mg/L，设置4组pH为6.5、7.5、8.5、9.5的模拟废水中培养小球藻。结果表明：pH为6.5~7.5时更适合氨氮的去除。随着pH值升高，pH>8.5时产生的高浓度游离氨对小球藻生长起主要抑制作用。基于藻细胞内叶绿素的积累，小球藻更偏好pH为6.5~8.5的实验环境。小球藻在pH=7.5时油脂产率达到最高为0.30 g/L/d，这与小球藻生长的适宜pH条件一致。综合考虑小球藻对氨氮的去除率及生长和油脂的积累情况，在利用小球藻处理沼液时，将pH控制在6.5~7.5区间内会更有利于氨氮的去除和废水的资源化利用。
- 小球藻 /
- 氨氮浓度 /
- pH /
- 生物质生产
Abstract: Biogas slurry contains appreciable levels of nitrogen, particularly ammonia that can lead to potential air and water quality problems. In this study, the ammonia-nitrogen treatment capacity and biomass utilization potential of Chlorella was tested at different pH levels. Chlorella was cultivated in simulated wastewater at four different pH levels (6.5, 7.5, 8.5, 9.5) with initial ammonia nitrogen concentration in the range of 120~130 mg/L. The results showed that the removal of ammonia nitrogen was better at pH 6.5~7.5. When pH increased more than 8.5, high concentration of free ammonia had a major inhibitory effect on the growth of Chlorella. In terms of chlorophyll accumulation in algal cells, Chlorella preferred a pH range from 6.5 to 8.5. The highest lipid producitivity of Chlorella (0.30 g/L/d) was achieved at pH7.5, which was consistent with the suitable pH condition for Chlorella growth. It is proposed that controlling pH value between 6.5~7.5 would be conduciue for removal of ammonia nitrogen and wastewater recycling when using Chlorella to treat biogas slurry.
- Chlorella /
- ammonia nitrogen concentration /
- pH /
- biomass production

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