中文核心期刊
CSCD来源期刊(核心库)
中国科技核心期刊
RCCSE中国核心学术期刊
JST China 收录期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

反硝化菌促进硒酸盐还原研究及其应用初探

韩郁林 石凌栋 赵和平

韩郁林, 石凌栋, 赵和平. 反硝化菌促进硒酸盐还原研究及其应用初探[J]. 环境工程, 2021, 39(11): 62-68,88. doi: 10.13205/j.hjgc.202111007
引用本文: 韩郁林, 石凌栋, 赵和平. 反硝化菌促进硒酸盐还原研究及其应用初探[J]. 环境工程, 2021, 39(11): 62-68,88. doi: 10.13205/j.hjgc.202111007
HAN Yu-lin, SHI Ling-dong, ZHAO He-ping. RESEARCH ON PROMOTION OF SELENIUM REDUCTION BY DENITRIFYING BACTERIA IN WASTEWATER AND ITS APPLICATION EXPLORATION[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(11): 62-68,88. doi: 10.13205/j.hjgc.202111007
Citation: HAN Yu-lin, SHI Ling-dong, ZHAO He-ping. RESEARCH ON PROMOTION OF SELENIUM REDUCTION BY DENITRIFYING BACTERIA IN WASTEWATER AND ITS APPLICATION EXPLORATION[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(11): 62-68,88. doi: 10.13205/j.hjgc.202111007

反硝化菌促进硒酸盐还原研究及其应用初探

doi: 10.13205/j.hjgc.202111007
基金项目: 

浙江省重点研发计划项目"市政污水高效深度处理及资源化利用技术装备研发"(2021C03171)。

详细信息
    作者简介:

    韩郁林(1998-),男,在读硕士研究生,主要研究方向为膜生物膜污水处理技术。hanyulin@zju.edu.cn

    通讯作者:

    赵和平(1978-),男,教授,主要研究方向为新型污水处理理论与技术。zhaohp@zju.edu.cn

RESEARCH ON PROMOTION OF SELENIUM REDUCTION BY DENITRIFYING BACTERIA IN WASTEWATER AND ITS APPLICATION EXPLORATION

  • 摘要: 生物还原法处理水体硒污染,绿色经济。但大多数硒酸盐还原模式菌环境适应性较差,工程应用困难。采用某些具有硒酸盐还原能力的反硝化菌治理硒污染,则是一种新的尝试。以反硝化菌主导的硒酸盐还原菌群为研究对象,通过底物还原速率对比分析,结合微生物群落数据和功能基因定量,证实了Nap型硝酸盐还原酶主导了硒酸盐还原过程,明确了反硝化菌在还原硒酸盐过程中的作用,进一步拓展了硒还原菌谱。并以此为基础,提出了一种全新的硒酸盐污染治理策略:采用硝酸盐对活性污泥进行预驯化,可大大提升硒酸盐还原效率,因而具有一定的工程应用价值。
  • [1] MORENO-Reyes R, SUETENS C, MATHIEU F, et al. Kashin-Beck osteoarthropathy in rural Tibet in relation to selenium and iodine status[J]. New England Journal of Medicine,1998,339(16):1112-1120.
    [2] STRANGES S, NAVAS-Acien A, RAYMAN M P. Selenium status and cardiometabolic health:state of the evidence[J].Nutrition, Metabolism and Cardiovascular Diseases,2010,20(10):754-760.
    [3] 王含锐,王长印,陈俊宇,等.含硒废水处理方法浅析[J].广州化工,2021,49(5):31-32

    ,37.
    [4] 刘芳.小球藻去除水体硒的影响机制研究[D].上海:上海交通大学,2017.
    [5] LEMLY D A. Aquatic selenium pollution is a global environmental safety issue[J]. Ecotoxicology and Environmental Safety,2004,59(1):44-56.
    [6] HE Y Z, XIANG Y J, ZHOU Y Y,et al. Selenium contamination, consequences and remediation techniques in water and soils:a review[J]. Environmental Research,2018,164:288-301.
    [7] LORTIE L,GOULD W D,RAJAN S,et al. Reduction of selenate and selenite to elemental selenium by a pseudomonas stutzeri isolate[J]. Applied and Environmental Microbiology,1992,58(12):4042-4044.
    [8] MICHIHIKO I,KAZUAKI T,TOMOKO F,et al. Selenate reduction by bacteria isolated from aquatic environment free from selenium contamination[J]. Water Research,2000,34(11):3019-3025.
    [9] 赖春宇. 氢气/甲烷作为电子供体驱动的硒酸盐和硝酸盐生物还原研究[D].杭州:浙江大学,2017.
    [10] LAI C Y,WEN L L,SHI L D,et al. Selenate and nitrate bioreductions using methane as the electron donor in a membrane biofilm reactor[J]. Environmental Science & Technology,2016,50(18):10179-10186.
    [11] KURODA M, YAMASHITA M, MIWA E, et al. Molecular cloning and characterization of the srdBCA operon, encoding the respiratory selenate reductase complex, from the selenate-reducing bacterium bacillus selenatarsenatis SF-1[J]. Journal of Bacteriology, 2011, 193(9):2141-2148.
    [12] MACY J M, RECH S, AULING G, et al. Thauera selenatis gen. nov. sp. nov. a member of the beta subclass of proteobacteria with a novel type of anaerobic respiration[J]. International Journal of Systematic Bacteriology, 1993, 43(1):135-142.
    [13] SRIDHAR V,YARED B S,WILLIAM A A,et al. Selenite reduction by a denitrifying culture:batch- and packed-bed reactor studies[J]. Applied Microbiology and Biotechnology,2006,71(6):953-962.
    [14] LUO J H,CHEN H,HU S H,et al. Microbial selenate reduction driven by a denitrifying anaerobic methane oxidation biofilm[J]. Environmental Science & Technology,2018,52(7):4006-4012.
    [15] YAN S,CHENG K Y,GINIGE M P,et al. Optimization of nitrate and selenate reduction in an ethanol-fed fluidized bed reactor via redox potential feedback control[J]. Journal of Hazardous Materials,2021,402:123770.
    [16] SHI L D, LV P L,WANG M,et al. A mixed consortium of methanotrophic archaea and bacteria boosts methane-dependent selenate reduction[J]. Science of the Total Environment,2020,732:139310.
    [17] DEMENTIN S, ARNOUX P, FRANGIONI B,et al. Access to the active site of periplasmic nitrate reductase:insights from site-directed mutagenesis and zinc inhibition studies[J]. Biochemistry,2007,46(34):9713-9721.
    [18] ZHAO H P,VAN G S,TANG Y N,et al. Interactions between perchlorate and nitrate reductions in the biofilm of a hydrogen-based membrane biofilm reactor[J]. Environmental Science & Technology,2011,45(23):10155-10162.
    [19] BRU D,SARR A,PHILIPPOT L. Relative abundances of proteobacterial membrane-bound and periplasmic nitrate reductases in selected environments[J]. Applied and Environmental Microbiology,2007,73(18):5971-5974.
    [20] JUAN C L G,SONIA H,STÉPHANIE H,et al. Quantification of a novel group of nitrate-reducing bacteria in the environment by real-time PCR[J]. Journal of Microbiological Methods,2004,57(3):399-407.
    [21] ZHAO H P, ONTIVEROS-VALENCIA A, TANG Y N,et al. Removal of multiple electron acceptors by pilot-scale, two-stage membrane biofilm reactors[J]. Water Research,2014,54:115-122.
    [22] LANGILLE M G I,ZANEVELD J,CAPORASO J G,et al. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences[J]. Nature Biotechnology,2013,31(9):814-821.
    [23] LIAO R H, LI Y, YU X M,et al. Performance and microbial diversity of an expanded granular sludge bed reactor for high sulfate and nitrate waste brine treatment[J]. Journal of Environmental Sciences,2014,26(4):743-753.
    [24] ARRUEBARRENA D P A,PEREYRA C M,MORENO R L,et al. Denitrification-derived nitric oxide modulates biofilm formation in Azospirillum brasilense[J]. FEMS Microbiology Letters,2013,338(1).
    [25] JEONGHWAN, JANG, YORIKO, et al. Potentially mobile denitrification functional genes identified in Azospirillum sp. Strain TSH58[J]. Applied and Environmental Microbiology, 2018.
    [26] MICHAEL F,JAN K,RUTH M,et al. Denitratisoma oestradiolicum gen. nov., sp. nov., a 17β-oestradiol-degrading, denitrifying betaproteobacterium[J]. International Journal of Systematic and Evolutionary Microbiology,2006,56(7):1547-1552.
    [27] PHILIPPOT L,HØJBERG O. Dissimilatory nitrate reductases in bacteria[J]. Biochimica et Biophysica Acta,1999,1446(1/2):1-23.
    [28] GATES A J,LUQUE-ALMAGRO V M,GODDARD A D,et al. A composite biochemical system for bacterial nitrate and nitrite assimilation as exemplified by Paracoccus denitrificans[J]. The Biochemical Journal,2011,435(3):743.
    [29] SABATY M,AVAZERI C,PIGNOL D,et al. Characterization of the reduction of selenate and tellurite by nitrate reductases[J]. Applied and Environmental Microbiology,2001,67(11):5122-5126.
    [30] AVAZÉRI,CÉCILE, RAYMOND J T,et al. Tellurite reductase activity of nitrate reductase is responsible for the basal resistance of Escherichia coli to tellurite[J]. Microbiology,1997,143(4):1181-1189.
  • 加载中
计量
  • 文章访问数:  434
  • HTML全文浏览量:  34
  • PDF下载量:  15
  • 被引次数: 0
出版历程
  • 收稿日期:  2021-06-29
  • 网络出版日期:  2022-01-26

目录

    /

    返回文章
    返回