微生物载体对MBBR工艺性能及微生物群落结构的影响

黎镛; 袁辉洲; 柯水洲; 祝凉; 李展鹏

doi:10.13205/j.hjgc.202112015

微生物载体对MBBR工艺性能及微生物群落结构的影响

doi: 10.13205/j.hjgc.202112015

黎镛^1,2,
袁辉洲^2, ,,
柯水洲¹,
祝凉^1,2,
李展鹏¹

1. 湖南大学土木工程学院, 长沙 410082;
2. 深圳职业技术学院建筑与环境工程学院, 广东深圳 518055

基金项目:

深圳市科技创新委基础研究项目(JCYJ20180305163710363)

深圳职业技术学院科技发展基金(6020320005K0)

深圳职业技术学院校科技项目(6020320005K)。

详细信息

作者简介:
黎镛(1996-),男,硕士,主要研究方向为生活污水处理技术。591725019@qq.com

通讯作者:
袁辉洲(1972-),女,副教授,主要从事污水处理与回用技术和环境生物技术方面的研究工作。653018949@qq.com

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出版历程
- 收稿日期: 2020-11-05
- 网络出版日期: 2022-03-30
- 刊出日期: 2022-03-30

EFFECTS OF MICROBIAL CARRIERS ON PERFORMANCE AND MICROBIAL COMMUNITY STRUCTURE OF MBBR

1. College of Civil Engineering, Hunan University, Changsha 410082, China;
2. Department of Construction and Environmental Engineering,Shenzhen Polytechnic, Shenzhen 518055, China

摘要

摘要: 通过选用聚氨酯吸水凝胶(porous polymer carriers, PPC)、聚氨酯海绵(polyurethane, PU)、聚丙烯(polypropylene, PP)3种材质的微生物载体,对分别投加这3种载体的移动床生物膜反应器(MBBR)的启动性能、氮去除性能及微生物群落结构进行对比分析。结果表明:在进水氨氮浓度为20 mg/L的情况下,PPC及PU载体表现出较好的挂膜启动性能和氨氮去除性能,出水氨氮平均浓度在5 mg/L左右,而PP载体的出水氨氮平均浓度将近7 mg/L,且去除效果稳定性较差;此外PU载体和PP载体的反硝化效果不理想,出水硝态氮平均浓度分别为6.07,4.87 mg/L,比PPC载体(2.80 mg/L)高出许多。微生物测序结果表明,微生物载体的选择对MBBR工艺微生物群落结构有一定影响,但Proteobacteria和Bacteroidetes始终是占比最大的细菌门类。属水平的分析表明PPC载体上存在较高丰度的反硝化Denitratisoma菌属,且PPC载体独特的结构和亲水性有助于营造缺氧环境,提高反应器整体脱氮性能和稳定性。
- 微生物载体 /
- 移动床生物膜反应器 /
- 微生物群落
Abstract: In this study, three kinds of microbial carriers including porous polymer carriers(PPC), polyurethane(PU) and polypropylene(PP) were selected to compare and analyze the start-up performance, nitrogen removal performance and microbial community structure of mobile bed biofilm reactor(MBBR) with three carriers respectively. The results showed that when the influent ammonia concentration was 20 mg/L, PPC and PU carriers showed better film-hanging start-up performance and ammonia nitrogen removal performance. The average effluent ammonia nitrogen concentration was about 5 mg/L, while the average effluent ammonia nitrogen concentration of PP carrier was nearly 7 mg/L, and the removal stability was poor. In addition, denitrification effect of PU and PP carrier was not ideal, and the average concentration of effluent nitrate nitrogen was 6.07, 4.87 mg/L, respectively, much higher than PPC carrier(2.80 mg/L). The microbial sequencing results showed that the selection of microbial carriers had a certain degree of influence on microbial community structure of the MBBR process, but Proteobacteria and Bacteroidetes were always the most dominant bacterial species. Analysis at the level of genus indicated that Denitratisoma bacteria with high abundance existed on PPC carrier, and the unique structure and hydrophilicity of PPC carrier contributed to the creation of anoxic environment, and improved the overall Denitratisoma performance and stability of the reactor where it was located.
- microbial carrier /
- mobile bed biofilm reactor /
- microbial community

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参考文献(25)

[1]	TANG B,YU C F,BIN L Y,et al.Essential factors of an integrated moving bed biofilm reactor-membrane bioreactor:adhesion characteristics and microbial community of the biofilm[J].Bioresource Technology,2016,211:574-583.
[2]	廖维,陈婷婷,李慧强,等.移动床生物膜反应器—膜生物反应器深度处理模拟废水及工业园区综合废水[J].环境污染与防治,2015,37(6):24-28.
[3]	陈莉,雷睿,周鹏飞,等.印染废水升级改造MBBR实验研究[J].环境科技,2017,30(1):39-42.
[4]	张晨华.小型生活污水新型填料AMC-PPC/MBBR处理工艺研究[D].西安:西安工程大学,2019.
[5]	WEIMER P J,MOEN G N.Quantitative analysis of growth and volatile fatty acid production by the anaerobic ruminal bacterium Megasphaera elsdenii T81[J].Applied Microbiology and Biotechnology,2013,97(9):4075-4081.
[6]	张玲玲,陈立,郭兴芳,等.南北方污水处理厂进水水质特性分析[J].给水排水,2012,48(1):45-49.
[7]	付昆明,杨宗玥,廖敏辉,等.中试MBBR反应器启动CANON工艺及其短程硝化[J].环境科学,2020,41(3):1393-1400.
[8]	廖榆敏,汤兵,陈秋雯.移动床生物反应器启动特性研究进展[J].水处理技术,2011,37(2):5-8.
[9]	张新波,张祖敏,宋姿,等.不同生物膜载体下MBBR中微生物群落变化特征[J].中国给水排水,2019,35(11):63-68.
[10]	刘辉.填料类型对MBBR启动及运行效能的影响[J].科技创新与应用,2019(21):69-70.
[11]	HAN S,LI X,LUO X S,et al.Nitrite-oxidizing bacteria community composition and diversity are influenced by fertilizer regimes,but are independent of the soil aggregate in acidic subtropical red soil[J].International Journal of Plant Sciences,2006,167(4):805-815.
[12]	王春香,刘常敬,郑林雪,等.厌氧氨氧化耦合脱氮系统中反硝化微生物研究[J].中国给水排水,2015,31(13):19-22.
[13]	吴桐,凌宇,王海燕,等.MBBR填料研究与应用进展[J].环境工程技术学报,2020,10(6):988-995.
[14]	郝志鹏,张会宁,彭维,等.悬浮填料生物膜反应器细菌群落季相更替对处理效果的稳定作用[J].环境工程学报,2019,13(11):2601-2609.
[15]	NGUYEN H T T,LE V Q,HANSEN A A,et al.High diversity and abundance of putative polyphosphate-accumulating Tetrasphaera-related bacteria in activated sludge systems[J].FEMS Microbiology Ecology,2011,76(2):256-267.
[16]	THOMSEN T R,KONG Y,NIELSEN P H.Ecophysiology of abundant denitrifying bacteria in activated sludge[J].FEMS Microbiology Ecology,2007,60(3):370-382.
[17]	MALLA S P,MICHAEL K,RICHARD R,et al.Transcriptional activity of paddy soil bacterial communities[J].Environmental Microbiology,2009,11(4):960-970.
[18]	司友斌,王娟.异化铁还原对土壤中重金属形态转化及其有效性影响[J].环境科学,2015,36(9):3533-3542.
[19]	辛晓东.运行条件对MBR膜污染调控的微生物学研究[D].哈尔滨:哈尔滨工业大学,2013.
[20]	BURRELL P,KELLER J,BLACKA LL L L.Characterisation of the bacterial consortium involved in nitrite oxidation in activated sludge[J].Water Science and Technology,1999,39(6):45-52.
[21]	DENG S H,LI D S,YANG X,et al.Biological denitrification process based on the Fe(0)-carbon micro-electrolysis for simultaneous ammonia and nitrate removal from low organic carbon water under a microaerobic condition[J].Bioresource Technology,2016,219:677-686.
[22]	MA Z,WEN X H,ZHAO F,et al.Effect of temperature variation on membrane fouling and microbial community structure in membrane bioreactor[J].Bioresource Technology,2013,133:462-468.
[23]	HEEYOUNG K,HANEUL K,YOCHAN J,et al.Ferruginibacter paludis sp.nov.,isolated from wetland freshwater,and emended descriptions of Ferruginibacter lapsinanis and Ferruginibacter alkalilentus [J]. International Journal of Systematic and Evolutionary Microbiology,2015,65(8):2635-2639.
[24]	GAO J T,ZHANG X L,YU J,et al.Cr(Ⅵ) removal performance and the characteristics of microbial communities influenced by the core-shell maifanite/ZnAl-layered double hydroxides (LDHs) substrates for chromium-containing surface water[J].Biochemical Engineering Journal,2020,160:107625.
[25]	EO J,NA Y,KIM M.Influence of rhinoceros beetle (Trypoxylus dichotomus septentrionalis) larvae and temperature on the soil bacterial community composition under laboratory conditions[J].Soil Biology and Biochemistry,2017,108:27-35.