生物炭缓解餐厨垃圾厌氧消化酸化的效果及机制

李旭升; 鹿莎莎; 江远琰; 王里奥

doi:10.13205/j.hjgc.202112027

生物炭缓解餐厨垃圾厌氧消化酸化的效果及机制

doi: 10.13205/j.hjgc.202112027

李旭升^1,2,
鹿莎莎^1,2,
江远琰^1,2,
王里奥^1,2, ,

1. 重庆大学煤矿灾害动力学与控制国家重点实验室, 重庆 400044;
2. 重庆大学环境与生态学院, 重庆 400044

基金项目:

社区垃圾源头智能分类与清洁收集技术及装备(2019YFC906100)。

详细信息

作者简介:
李旭升(1996-),男,硕士研究生,主要研究方向为水污染控制。478035885@qq.com

通讯作者:
王里奥(1956-),女,教授,主要研究方向为固体废物污染控制及资源化利用。wangliao@cqu.edu.cn

计量
- 文章访问数: 222
- HTML全文浏览量: 36
- PDF下载量: 7
- 被引次数: 0
出版历程
- 收稿日期: 2021-03-18
- 网络出版日期: 2022-03-30
- 刊出日期: 2022-03-30

EFFECT AND MECHANISM OF BIOCHAR IN MITIGATING ACIDIFICATION OF ANAEROBIC DIGESTION PROCESS FOR FOOD WASTE

LI Xu-sheng^1,2,
LU Sha-sha^1,2,
JIANG Yuan-yan^1,2,
WANG Li-ao^{1,2
, ,}

1. State Key Laboratory of Coal Mine Disaster Dynamics and Control, Chongqing University, Chongqing 400044, China;
2. College of Environment and Ecology, Chongqing University, Chongqing 400044, China

摘要

摘要: 研究了在高有机负荷(30 g VS/L,VS为挥发性固体含量)下生物炭缓解餐厨垃圾厌氧消化酸化,促进产甲烷的效应及机制。结果表明:碱性多孔生物炭在最优添加量下(1 g/g VS),反应20 d时,累积产甲烷量达到312.40 mL/(g·VS),与对照组相比提升了101.7%,同时产甲烷停滞期缩短62%。并在酸化最严重时挥发性脂肪酸(VFA)含量降低1151.28 mg/L。研究结果表明:生物炭的多孔结构是促进挥发性脂肪酸分解的关键因素,碱度和营养物质可以起到促进作用。高通量测序结果表明:最佳添加量下甲烷丝菌属(Methanothrix)、拟杆菌(Bacteroidales)、梭菌(Clostridiales)的相对丰度分别由26.12%、43.08%和9.95%提高到46.05%、56.25%和12.20%。生物炭缓解餐厨垃圾消化酸化的机制是为微生物提供反应场所,增强了微生物间的电子传递,提高了厌氧微生物的呼吸速率。
- 厌氧消化 /
- 生物炭 /
- 餐厨垃圾 /
- 种间电子传递 /
- 酸化
Abstract: A stimulated experiment was carried out to investigate the promotion of anaerobic digestion of kitchen waste by biochar at high organic loads(30 g VS/L). The results indicated that the cumulative methane production was increased by 101.7% to 312.40 mL CH₄/g VS after 20 d at the optimum addition of alkaline porous biochar(1 g/g VS), while the lag phase for methane production was reduced by 62% as compared to the control group. Besides, the addition of biochar reduced the volatile fatty acid(VFA) content by 1151.28 mg/L at the most severe acidifying stage. Mechanism exploration disclosed that the porous structure of biochar exerted vital influence on the decomposition of VFA, and alkalinity and nutrients also played an important role in the VFA degradation. The results of high-throughput sequencing showed that the relative abundance of Methanothrix, Bacteroidales and Clostridiales were increased from 26.12%, 43.08% and 9.95% to 46.05%, 56.25% and 12.20%, respectively. The underlying mechanism for biochar affecting the digestion were providing reaction sites for microorganisms, enhancing electron transfer among microorganisms, and accelerating the respiration rate of the microorganisms.
- anaerobic digestion /
- biochar /
- food waste /
- interspecies electron transfer /
- acidification

HTML全文

参考文献(54)

[1]	A S M,B A K P,A P K O,et al.Co-digestion of food waste and sewage sludge for methane production:current status and perspective[J].Bioresource Technology,2018,265:519-531.
[2]	BI Z J,TAI J.Analysis on wet waste treatment process technology and enabling policies in China:a case study of Shanghai[J].Environment and Sustainable Development,2019,4:54-58.
[3]	ZHOU Y,ENGLER N,NELLES M.Symbiotic relationship between hydrothermal carbonization technology and anaerobic digestion for food waste in China[J].Bioresource Technology,2018,260:404-412.
[4]	HAO X D,WEI J,VAN LOOSDRECHT M C M,et al.Analysing the mechanisms of sludge digestion enhanced by iron[J].Water Research,2017,117:58-67.
[5]	GUPTA P,SINGH R S,SACHAN A,et al.Study on biogas production by anaerobic digestion of garden-waste[J].Fuel,2012,95(1):495-498.
[6]	ALMOMANI F,BHOSALE R R.Enhancing the production of biogas through anaerobic co-digestion of agricultural waste and chemical pre-treatments[J].Chemosphere,2020,255:126805.
[7]	JAYANTH T A S,MAMINDLAPELLI N K,BEGUM S,et al.Anaerobic mono and co-digestion of organic fraction of municipal solid waste and landfill leachate at industrial scale:Impact of volatile organic loading rate on reaction kinetics,biogas yield and microbial diversity[J].Science of the Total Environment,2020,748:142462.
[8]	何强,吉芳英,李家杰.污泥处理处置及资源化途径与新技术[J].给水排水,2016,42(2):2-4.
[9]	孙健,罗文邃,汪洋.园林植物废弃物资源化利用现状与展望[C]//2013中国环境科学学会学术年会,昆明,2013.
[10]	CHEN Y,CHENG J J,CREAMER K S.Inhibition of anaerobic digestion process:a review[J].Bioresource Technology,2008,99(10):4044-4064.
[11]	VELUCHAMY C,KALAMDHAD A S.Effect of Total Solid Content of Lignocellulose Pulp and Paper Mill Sludge on Methane Production and Modeling[J].Journal of Environmental Engineering,2020,146(3).
[12]	VONGVICHIANKUL C,DEEBAO J,KHONGNAKORN W.Relationship between pH,oxidation reduction potential (ORP) and biogas production in mesophilic screw anaerobic digester[C]//WAEWSAK J,SANGKHARAK K,OTHONG S,et al.2017 International Conference on Alternative Energy In Developing Countries And Emerging Economies,2017:877-882.
[13]	ZHENG Z H,CAI Y F,ZHANG Y,et al.The effects of C/N (10-25) on the relationship of substrates,metabolites,and microorganisms in "inhibited steady-state" of anaerobic digestion[J].Water Research,2021,188:116466.
[14]	RAJAGOPAL R,MASSE D I,SINGH G.A critical review on inhibition of anaerobic digestion process by excess ammonia[J].Bioresource Technology,2013,143:632-641.
[15]	YENIGUN O,DEMIREL B.Ammonia inhibition in anaerobic digestion:a review[J].Process Biochemistry,2013,48(5/6):901-911.
[16]	AHOU Y S,ANGELI J-R B,AWAD S,et al.Assessment of inoculum to substrate ratio to recover energy from cassava wastes through anaerobic digestion[J].Waste and Biomass Valorization,2020,12:1891-1900.
[17]	ZHANG W L,LI L T,XING W L,et al.Dynamic behaviors of batch anaerobic systems of food waste for methane production under different organic loads,substrate to inoculum ratios and initial pH[J].Journal of Bioscience and Bioengineering,2019,128(6):733-743.
[18]	WANG Y Y,ZHANG Y L,WANG J B,et al.Effects of volatile fatty acid concentrations on methane yield and methanogenic bacteria[J].Biomass & Bioenergy,2009,33(5):848-853.
[19]	HUA Y,CAI C,DAI X H.A specious relevance between theory and practice:Insights into temperature parameter and multi-phase strategy of anaerobic digestion of straw[J].Science of the Total Environment,2021,753:142212.
[20]	AWE O W,ZHAO Y Q,NZIHOU A,et al.A review of biogas utilisation,purification and upgrading technologies[J].Waste And Biomass Valorization,2017,8(2):267-283.
[21]	ZHOU H Q,WEN Z Y.Solid-state anaerobic digestion for waste management and biogas production[J].Advances in Biochemical Engineering/Biotechnology,2019,169:147-168.
[22]	SUNYOTO N M S,ZHU M M,ZHANG Z Z,et al.Effect of biochar addition on hydrogen and methane production in two-phase anaerobic digestion of aqueous carbohydrates food waste[J].Bioresource Technology,2016,219:29-36.
[23]	JIANG Q,CHEN Y D,YU S K,et al.Effects of citrus peel biochar on anaerobic co-digestion of food waste and sewage sludge and its direct interspecies electron transfer pathway study[J].Chemical Engineering Journal,2020,398:125643.
[24]	LIN C Y,LAY C H.Effects of carbonate and phosphate concentrations on hydrogen production using anaerobic sewage sludge microflora[J].International Journal of Hydrogen Energy,2004,29(3):275-281.
[25]	YUAN T G,KO J H,ZHOU L L,et al.Iron oxide alleviates acids stress by facilitating syntrophic metabolism between Syntrophomonas and methanogens[J].Chemosphere,2020,247:125866.
[26]	LEI Y Q,SUN D Z,DANG Y,et al.Stimulation of methanogenesis in anaerobic digesters treating leachate from a municipal solid waste incineration plant with carbon cloth[J].Bioresource Technology,2016,222:270-276.
[27]	ROTARU A E,CALABRESE F,STRYHANYUK H,et al.Conductive particles enable syntrophic acetate oxidation between geobacter and methanosarcina from coastal sediments[J].Mbio,2018,9(3).
[28]	QAMBRANI N A,RAHMAN M M,WON S,et al.Biochar properties and eco-friendly applications for climate change mitigation,waste management,and wastewater treatment:a review[J].Renewable & Sustainable Energy Reviews,2017,79:255-273.
[29]	SCHULZ H,GLASER B.Effects of biochar compared to organic and inorganic fertilizers on soil quality and plant growth in a greenhouse experiment[J].Journal of Plant Nutrition and Soil Science,2012,175(3):410-422.
[30]	COONEY M J,LEWIS K,HARRIS K,et al.Start up performance of biochar packed bed anaerobic digesters[J].Journal of Water Process Engineering,2016,9:e7-e13.
[31]	MASEBINU S O,AKINLABI E T,MUZENDA E,et al.A review of biochar properties and their roles in mitigating challenges with anaerobic digestion[J].Renewable and Sustainable Energy Reviews,2019,103:291-307.
[32]	KAUR G,JOHNRAVINDAR D,WONG J W C.Enhanced volatile fatty acid degradation and methane production efficiency by biochar addition in food waste-sludge co-digestion:a step towards increased organic loading efficiency in co-digestion[J].Bioresour Technol,2020,308:123250.
[33]	KONG X,WEI Y H,XU S,et al.Inhibiting excessive acidification using zero-valent iron in anaerobic digestion of food waste at high organic load rates[J].Bioresource Technology,2016,211:65-71.
[34]	PYTLAK A,KASPRZYCKA A,SZAFRANEK-NAKONIECZNA A,et al.Biochar addition reinforces microbial interspecies cooperation in methanation of sugar beet waste (pulp)[J].Science of the Total Environment,2020,730:138921.
[35]	GUI X Y,LIU C,LI F Y,et al.Effect of pyrolysis temperature on the composition of DOM in manure-derived biochar[J].Ecotoxicology and Environmental Safety,2020,197:110597.
[36]	NATH K,DAS D.Modeling and optimization of fermentative hydrogen production[J].Bioresource Technology,2011,102(18):8569-8581.
[37]	SHEN Y W,LINVILLE J L,URGUN-DEMIRTAS M,et al.Producing pipeline-quality biomethane via anaerobic digestion of sludge amended with corn stover biochar with in-situ CO₂ removal[J].Applied Energy,2015,158:300-309.
[38]	MA J Y,CHEN F F,XUE S X,et al.Improving anaerobic digestion of chicken manure under optimized biochar supplementation strategies[J].Bioresource Technology,2021,325:124697.
[39]	KIZITO S,WU S B,KIRUI W K,et al.Evaluation of slow pyrolyzed wood and rice husks biochar for adsorption of ammonium nitrogen from piggery manure anaerobic digestate slurry[J].Science of the Total Environment,2015,505:102-112.
[40]	INDREN M,BIRZER C H,KIDD S P,et al.Effect of total solids content on anaerobic digestion of poultry litter with biochar[J].Journal of Environmental Management,2020,255:109744.
[41]	LUO C H,LÜ F,SHAO L M,et al.Application of eco-compatible biochar in anaerobic digestion to relieve acid stress and promote the selective colonization of functional microbes[J].Water Research,2015,68:710-718.
[42]	SUMMERS Z M,FOGARTY H E,LEANG C,et al.Direct Exchange of electrons within aggregates of an evolved syntrophic coculture of anaerobic bacteria[J].Science,2010,330(6009):1413-1415.
[43]	李坚,汤佳,庄莉,等.导电性生物炭促进Geobacter和Methanosarcina共培养体系互营产甲烷过程[J].生态环境学报,2018,27(7):1260-1268.
[44]	MOSTAFA A,IM S,SONG Y C,et al.Enhanced anaerobic digestion of long chain fatty acid by adding magnetite and carbon nanotubes[J].Microorganisms,2020,8(3).
[45]	ROTARU A E,SHRESTHA P M,LIU F,et al.A new model for electron flow during anaerobic digestion:direct interspecies electron transfer to Methanosaeta for the reduction of carbon dioxide to methane[J].Energy Environmental Science,2014,7(1):408-415.
[46]	ZHAO Z Q,ZHANG Y B,LI Y,et al.Potentially shifting from interspecies hydrogen transfer to direct interspecies electron transfer for syntrophic metabolism to resist acidic impact with conductive carbon cloth[J].Chemical Engineering Journal,2017,313:10-18.
[47]	CRUZ VIGGI C,ROSSETTI S,FAZI S,et al.Magnetite particles triggering a faster and more robust syntrophic pathway of methanogenic propionate degradation[J].Environmental Science & Technology,2014,48(13):7536-7543.
[48]	ROTARU A E,SHRESTHA P M,LIU F,et al.Direct interspecies electron transfer between Geobacter metallireducens and Methanosarcina barkeri[J].Applied and Environmental Microbiology,2014,80(15):4599-4605.
[49]	HOLMES D E,SHRESTHA P M,WALKER D,et al.Metatranscriptomic evidence for direct interspecies electron transfer between geobacter and methanothrix species in methanogenic rice paddy soils[J].Applied & Environmental Microbiology,2017,83(9):AEM.00223-17.
[50]	ZHAO Z Q,ZHANG Y B,HOLMES D E,et al.Potential enhancement of direct interspecies electron transfer for syntrophic metabolism of propionate and butyrate with biochar in up-flow anaerobic sludge blanket reactors[J].Bioresource Technology,2016,209:148-156.
[51]	任南琪,赵丹,陈晓蕾,等.厌氧生物处理丙酸产生和积累的原因及控制对策[J].中国科学(B辑化学),2002,32(1):83-89.
[52]	ZHANG J S,WANG Q Q,ZHENG P W,et al.Anaerobic digestion of food waste stabilized by lime mud from papermaking process[J].Bioresource Technology,2014,170:270-277.
[53]	LÜ F,LUO C H,SHAO L M,et al.Biochar alleviates combined stress of ammonium and acids by firstly enriching Methanosaeta and then Methanosarcina[J].Water Research,2016,90:34-43.
[54]	POEHLEIN A,SCHNEIDER D,SOH M,et al.Comparative genomic analysis of members of the genera Methanosphaera and Methanobrevibacter reveals distinct clades with specific potential metabolic functions[J].Archaea-an International Microbiological Journal,2018,2018.