复合人工湿地系统对低污染水总氮的净化效果及其微生物群落结构特征

余俊霞; 陈双荣; 刘凌言; 李莹美; 陆轶峰; 马兰

doi:10.13205/j.hjgc.202201003

复合人工湿地系统对低污染水总氮的净化效果及其微生物群落结构特征

doi: 10.13205/j.hjgc.202201003

1. 云南大学国际河流与生态安全研究院, 昆明 650000;
2. 云南大学生态与环境学院, 昆明 650000;
3. 云南省科学技术发展研究院, 昆明 650000

基金项目:

高原湖泊水环境治理与功能提升技术研究与集成及应用示范(2018BC001)

详细信息

作者简介:
余俊霞(1995-),女,硕士研究生,主要研究方向为水环境治理。1432878073@qq.com

通讯作者:
陆轶峰(1971-),男,副教授。lyftoday@126.com

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- 文章访问数: 149
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- 被引次数: 0
出版历程
- 收稿日期: 2021-01-08
- 网络出版日期: 2022-03-30
- 刊出日期: 2022-03-30

PURIFICATION EFFECT OF TOTAL NITROGEN IN LOW-POLLUTED WATER BY A COMPOSITE CONSTRUCTED WETLAND SYSTEM AND ITS MICROBIAL COMMUNITY STRUCTURE

1. Institute of International Rivers And Eco-Security, Yunnan University, Kunming 650000, China;
2. School of Ecology and Environmental Science, Yunan University, Kunming 650000, China;
3. Yunnan Academy of Science and Technology Development, Kunming 650000, China

摘要

摘要: 为了解复合人工湿地系统对低污染水中总氮的处理效果及微生物群落结构特征,以表流-潜流-沉水植物塘构建的复合人工湿地系统为研究对象,研究该系统对低污染水中TN、NH₄⁺-N、NO₃^--N、NO₂^--N的去除效果,并采用高通量测序揭示各级单元微生物群落结构与差异性。结果表明:复合人工湿地系统在0.12~0.24 m³/(m²·d)的水力负荷下出水达到GB 3838—2002《地表水环境质量标准》Ⅳ类水质,对污染物均有较好的去除效果,对TN、NH₄⁺-N的去除率为62.35%~76.86%、36.94%~74.68%。各级单元主要的脱氮微生物表现出较大差异,属水平上,表流湿地主要脱氮菌群为硝化螺旋菌属、996-1属和生丝菌属;潜流湿地主要脱氮菌群为贪铜菌属和不动杆菌属;沉水植物塘主要脱氮菌群为甲基娇养杆菌属和嗜甲基菌属。说明各单元对总氮的去除效果均有贡献且存在差异。
- 总氮 /
- 微生物多样性 /
- 复合人工湿地 /
- 低污染水
Abstract: To understand the treatment effect of total nitrogen in low-polluted wastewater by composite constructed wetland system and the characteristics of microbial community structure in the constructed wetland consisted of surface flow-subsurface flow-submerged plant pond, we researched the removal rates of TN, NH₄⁺-N, NO₃^--N and NO₂^--N in the wastewater by the system. High throughput sequencing method was applied to reveal the microbial community structure and diversity in the units at all levels. The results showed that the effluent of the composite constructed wetland system reached the class Ⅳ of Environmental Quality Standard for Surface Water under the hydraulic load of 0.12~0.24 m³/(m² · d), and had a good removal effect on pollutants. And the removal rate of TN, NH₄⁺-N was 62.35%~76.86% and 36.94%~74.68%. The main denitrification microorganisms in each unit showed great differences. At the genus level, the main denitrification microorganisms in the surface flow wetland were Nitrospira, 996-1 and Hyphomicrobium. The main denitrification bacteria groups in the subsurface flow wetland were Cupriavidus and Acinetobacter. The main denitrification microorganisms in the submerged plant pond were Methylotenera and Methylophilus. The results indicated that all the units contributed to the removal of total nitrogen but there were differences between them.
- total nitrogen /
- microbial diversity /
- composite constructed wetland /
- low-polluted water

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参考文献(44)

[1]	MCCOMB A J.Eutrophic Shallow Estuaries and Lagoons[M].Boca Raton,FL:CRC Press,1995:126-127.
[2]	LIVINGSTONI R J.Eutrophication Processes in Coastal Systems:Origin and Succession of Plankton Blooms and Effects on Secondary Production in Gulf Coast Estuaries[M].Boca Raton,FL:CRC Press,2002:145-147.
[3]	STROGANOVA M,MYAGKOVA A,PROKOFIEVA T,et al.Soil of Moscow and Urban Environment[M].Moscow:Russian Federation Press,1998:1-171.
[4]	凌子微,仝欣楠,李亚红,等.处理低污染水的复合人工湿地脱氮过程[J].环境科学研究,2013,26(3):320-325.
[5]	薛利红,杨林章.太湖流域稻田湿地对低污染水中氮磷的净化效果[J].环境科学研究,2015,28(1):117-124.
[6]	YANG C,LI L,SHI J L,et al.Advanced treatment of textile dyeing secondary effluent using magnetic anion exchange resin and its effect on organic fouling in subsequent RO membrane[J].Journal of Hazardous Materials,2015,284:50-57.
[7]	XU M,LIU W J,LI C,et al.Evaluation of the treatment performance and microbial communities of a combined constructed wetland used to treat industrial park wastewater[J].Environmental Science and Pollution Research,2016,23(11):10990-11001.
[8]	杨婷,李翠梅,张燕,等.新型多级复合人工湿地的应用与除磷效果[J].环境工程学报,2016,10(11):6235-6240.
[9]	VYMAZAL J.Constructed wetlands for treatment of industrial wastewaters:a review[J].Ecological Engineering,2014,73:724-751.
[10]	张玲玲,杨永强,张权,等.组合型人工湿地对二级好氧单元出水的深度处理[J].环境工程学报,2019,13(7):1592-1601.
[11]	熊家晴,段然,郑于聪,等.不同形式复合人工湿地对污染河水的净化差异研究[J].工业水处理,2017,37(10):27-30.
[12]	ZHANG S Y,ZHOU Q H,XU D,et al.Vertical-flow constructed wetlands applied in a recirculating aquaculture system for channel catfish culture:effects on water quality and zooplankton[J].Polish Journal of Environmental Studies,2010,19(5):1063-1070.
[13]	AYAZ S Ç,AKTAŞ Ö,FINDIK N,et al.Effect of recirculation on nitrogen removal in a hybrid constructed wetland system[J].Ecological Engineering,2012,40:1-5.
[14]	XIE X H,HE F,XU D,et al.Application of large-scale integrated vertical-flow constructed wetland in Beijing Olympic Forest Park:design,operation and performance[J].Water and Environment Journal,2012,26(1):100-107.
[15]	韩梅,周小平,程媛华,等.环太湖主要河流氮素组成特征及来源[J].环境科学研究,2014,27(12):1450-1457.
[16]	丁怡,唐海燕,刘兴坡,等.不同类型人工湿地在污水脱氮中的研究进展[J].工业水处理,2019,39(7):1-3 ,9.
[17]	葛媛,郑于聪,王怡雯,等.复合人工湿地在水处理中的应用进展[J].环境科学与技术,2018,41(1):99-108.
[18]	林运通,崔理华,范远红,等.5种湿地沉水植物对模拟污水厂尾水的深度处理[J].环境工程学报,2016,10(12):6914-6922.
[19]	LIU W R,CHEN W J,YANG D H,et al.Functional and compositional characteristics of nitrifiers reveal the failure of achieving mainstream nitritation under limited oxygen or ammonia conditions[J].Bioresource Technology,2019,275:272-279.
[20]	LIANG H W,GAO M,LIU J X,et al.A novel integrated step-feed biofilm process for the treatment of decentralized domestic wastewater in rural areas of China[J].Journal of Environmental Sciences,2010,22(3):321-327.
[21]	VYMAZAL J.The use of hybrid constructed wetlands for wastewater treatment with special attention to nitrogen removal:a review of a recent development[J].Water Research,2013,47(14):4795-4811.
[22]	周元清,李秀珍,李淑英,等.不同类型人工湿地微生物群落的研究进展[J].生态学杂志,2011,30(6):1251-1257.
[23]	LI X,ZHANG M M,LIU F,et al.Abundance and distribution of microorganisms involved in denitrification in sediments of a Myriophyllum elatinoides purification system for treating swine wastewater[J].Environmental Science and Pollution Research,2015,22(22):17906-17916.
[24]	LIU F,ZHANG S N,WANG Y,et al.Nitrogen removal and mass balance in newly-formed Myriophyllum aquaticum mesocosm during a single 28-day incubation with swine wastewater treatment[J].Journal of Environmental Management,2016,166:596-604.
[25]	MANZ W,WAGNER M,AMANN R,et al.In-situ characterization of the microbial consortia active in 2 waste-water treatment plants[J].Water Research,1994,28(8):1715-1723.
[26]	MIAO Y,LIAO R X,ZHANG X X,et al.Metagenomic insights into Cr(Ⅵ) effect on microbial communities and functional genes of an expanded granular sludge bed reactor treating high-nitrate wastewater[J].Water Research,2015,76:43-52.
[27]	潘傲,张智,孙磊,等.种植不同植物的表面流人工湿地净化效果和微生物群落差异分析[J].环境工程学报,2019,13(8):1918-1929.
[28]	ZHANG Y Y,DONG J D,YANG B,et al.Bacterial community structure of mangrove sediments in relation to environmental variables accessed by 16S rRNA gene-denaturing gradient gel electrophoresis fingerprinting[J].Scientia Marina,2009,73(3):487-498.
[29]	LI X,LI Y Y,LI Y,et al.Diversity and distribution of bacteria in a multistage surface flow constructed wetland to treat swine wastewater in sediments[J].Applied Microbiology and Biotechnology,2018,102(24):10755-10765.
[30]	房昀昊,彭剑峰,宋永会,等.高通量测序法表征潜流人工湿地中不同植物根际细菌群落特征[J].环境科学学报,2018,38(3):911-918.
[31]	宋旭,林陶,夏品华,等.沉水植物附植生物膜藻类组成及重金属累积特征[J].湖泊科学,2019,31(5):1268-1278.
[32]	MISHRA A K,KAUSHIK M S,TIWARI D N.Nitrogenase and hydrogenase:enzymes for nitrogen fixation and hydrogen production in cyanobacteria[J].Cyanobacteria,2019:173-191.
[33]	纪海婷,谢冬,周恒杰,等.沉水植物附植生物群落生态学研究进展[J].湖泊科学,2013,25(2):163-170.
[34]	WANG Q,XIE H,HUU H N,et al.Microbial abundance and community in subsurface flow constructed wetland microcosms:role of plant presence[J].Environmental Science and Pollution Research,2016,23(5):1-10.
[35]	SCHREIER H J,MIRZOYAN N,SAITO K.Microbial diversity of biological filters in recirculating aquaculture systems[J].Current Opinion in Biotechnology,2010,21(3):318-325.
[36]	LI L Z,HE C G,JI G D,et al.Nitrogen removal pathways in a tidal flow constructed wetland under flooded time constraints[J].Ecological Engineering,2015,81:266-271.
[37]	LIU H,LU Q,WANG Q,et al.Isolation of a bacterial strain,Acinetobacter sp.from centrate wastewater and study of its cooperation with algae in nutrients removal[J].Bioresource Technology,2017,235:59-69.
[38]	梁康,王启烁,王飞华,等.人工湿地处理生活污水的研究进展[J].农业环境科学学报,2014,33(3):422-428.
[39]	LI C,REN H Q,XU M,et al.Study on anaerobic ammonium oxidation process coupled with denitrification microbial fuel cells (MFCs) and its microbial community analysis[J].Bioresource Technology,2015,175:545-552.
[40]	XU W W,WANG L M,PENG F Q,et al.Spatiotemporal distribution and interaction of denitrifying functional genes in a novel DAS-NUA biofilter used for groundwater nitrate treatment[J].Science of the Total Environment,2020,712:136595.
[41]	SUN Z Y,LV Y K,LIU Y X,et al.Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel metal resistant bacterium Cupriavidus sp.S1[J].Bioresource Technology,2016,220:142-150.
[42]	YAO S,NI J R,MA T,et al.Heterotrophic nitrification and aerobic denitrification at low temperature by a newly isolated bacterium,Acinetobacter sp.HA2[J].Bioresource Technology,2013,139(13):80-86.
[43]	BECK D A C,HENDRICKSON E L,VOROBEVA,et al.An in tegrated proteomics/transcriptomics approach points to oxygen as the main electron sink for methanol metabolism in Methylotenera mobilis[J].Journal of Bacteriology,2011,193(18):4758-4765.
[44]	宋淑芬,杨帆,高绣纺,等.湿地法处理生活污水对仙桃湿地地表水微生物群落结构的影响[J].微生物学通报,2019,46(3):512-521.