牛粪好氧堆肥发酵微生物群落结构演替与环境因子和腐熟度的相关性

李文兵; 毕江涛; 刘鹏; 惠治兵; 孙权

doi:10.13205/j.hjgc.202201011

牛粪好氧堆肥发酵微生物群落结构演替与环境因子和腐熟度的相关性

doi: 10.13205/j.hjgc.202201011

1. 宁夏大学农学院, 银川 750021;
2. 宁夏大学生态环境学院, 银川 750021

基金项目:

宁夏回族自治区重点研发计划(2018ZDKJ0312)

详细信息

作者简介:
李文兵(1995-),男,硕士研究生,主要研究方向为环境微生物。1323234800@qq.com

通讯作者:
毕江涛(1964-),男,博士,研究员,主要研究方向为环境微生物与环境修复工程。Bi_jt@nxu.edu.cn

计量
- 文章访问数: 362
- HTML全文浏览量: 49
- PDF下载量: 6
- 被引次数: 0
出版历程
- 收稿日期: 2021-03-18
- 网络出版日期: 2022-03-30
- 刊出日期: 2022-03-30

CORRELATION BETWEEN THE SUCCESSION OF MICROBIAL COMMUNITY STRUCTURE AND ENVIRONMENTAL FACTORS AND MATURITY OF CATTLE MANURE AEROBIC COMPOSTING

1. School of Agriculture, Ningxia University, Yinchuan 750021, China;
2. School of Ecology Environment, Ningxia University, Yinchuan 750021, China

摘要

摘要: 为了解好氧堆肥发酵过程中微生物群落结构变化规律和腐熟度生物学指标研究,以自然发酵牛粪为研究材料,利用高通量测序技术分析细菌群落结构在发酵不同阶段、层次上的动态变化与环境因子和腐熟度的相互关系。结果显示:堆体上层温度、碳氮比和种子发芽指数指标优于下层;整个堆肥发酵过程中,细菌丰度和多样性指数呈先升高后降低的趋势;在门水平上,厚壁菌门(Firmicutes)在整个堆肥过程占据主导地位;在属水平上,升温期优势菌属为未鉴定的根瘤菌属(unidentified_Rhizobiaceae),高温期为芽孢杆菌属(Bacillus),降温腐熟期为诺卡氏菌属(Nocardiopsis);Spearman相关性分析显示Bacillus与有机质、碳氮比、含水率和温度间呈极显著正相关(P<0.01);CCA分析表明Nocardiopsis可作为腐熟期指示微生物,OM是堆肥第1影响因子;拟合回归方程分析得出,Chao1指数与腐熟度指标存在较强的相关性(R²=0.986)。
- 牛粪 /
- 好氧堆肥 /
- 群落结构 /
- 环境因子 /
- 细菌
Abstract: To understand the change of microbial community structure in the process of aerobic composting fermentation and study the biological index of ripening degree, in the trial we took cow dung as the samples, and by high-throughput sequencing, the correlation between the dynamic change of microbial community structure and environmental factors and maturity at different stages and in high and low profiles was analyzed. The results showed that the temperature, C/N and seed germination index condition was better in the upper layer than the lower layer. For the whole composting period, the bacterial abundance and diversity showed a trend of first increasing and then decreasing; the bacterial community structure was at the phylum level that Firmicutes dominated the entire composting process; at the genus level, in the heating period the dominant species was unidentified_Rhizobiaceae, in high temperature period the dominant species was Bacillus, in cooling period the dominant species wsa Nocardiopsis; Spearman correlation analysis showed that there was a very significant positive correlation(P<0.01) between Bacillus and organic matters, C/N, water content and temperature; canonical correlation analysis showed that Nocardiopsis could be used as an indicator microorganism, and OM was the prominent influencing factor; the analysis of the fitted regression equation showed that there was a strong correlation between the Chao1 index and the maturity index(R²=0.986).
- cow dung /
- aerobic composting /
- community structure /
- environmental factors /
- bacteria

HTML全文

参考文献(39)

[1]	ZHONG X,LI X X,ZENG Y,et al.Dynamic change of bacterial community during dairy manure composting process revealed by high-throughput sequencing and advanced bioinformatics tools[J].Bioresource Technology,2020,306:123091-123101.
[2]	ABDELLAH Y,LI T Z,CHEN X,et al.Role of psychrotrophic fungal strains in accelerating and enhancing the maturity of pig manure composting under low-temperature conditions[J].Bioresource Technology,2021,320:124402-124415.
[3]	NADIA O,XIANG L Y,LIE L Y,et al.Investigation of physico-chemical properties and microbial community during poultry manure co-composting process[J].Journal of Environmental Sciences,2015,28:81-94.
[4]	蔡涵冰,冯雯雯,董永华,等.畜禽粪便和桃树枝工业化堆肥过程中微生物群演替及其与环境因子的关系[J].环境科学,2020,41(2):997-1004.
[5]	许修宏,成利军,许本姝,等.基于高通量测序分析牛粪堆肥中细菌群落动态变化[J].东北农业大学学报,2018,49(3):10-20.
[6]	黄雅楠,王晓慧,曹琦,等.高通量测序技术分析猪粪堆肥过程中微生物群落结构变化[J].微生物学杂志,2018,38(5):21-26.
[7]	张晶,孙照勇,钟小忠,等.奶牛粪条垛式模拟堆肥腐熟度及微生物群落结构变化[J].应用与环境生物学报,2016,22(3):423-429.
[8]	沈玉君,李冉,孟海波,等.国内外堆肥标准分析及其对中国的借鉴启示[J].农业工程学报,2019,35(12):265-271.
[9]	董存明,张曼,邓小垦,等.不同碳氮比条件下鸡粪和椰糠高温堆肥腐熟过程研究[J].生态与农村环境学报,2015,31(3):420-424.
[10]	BAI L,DENG Y,LI J,et al.Role of the proportion of cattle manure and biogas residue on the degradation of lignocellulose and humification during composting[J].Bioresource Technology,2020,307:122941-122950.
[11]	何京钟,黄钧,赵晶桃,等.微生物菌剂堆肥中草药渣中试研究[J].应用与环境生物学报,2015,21(5):860-865.
[12]	LI R H,WANG J W,ZHANG Z Q,et al.Nutrient transformations during composting of pig manure with bentonite[J].Bioresource Technology,2012,121:361-368.
[13]	陈雅娟,霍培书,韩卉,等.鸡粪锯末好氧堆肥过程中硝化细菌动态变化[J].中国农业大学学报,2014,19(2):100-107.
[14]	李荣华,张广杰,张增强,等.添加木炭改善猪粪稻壳好氧堆肥工艺及质量[J].农业工程学报,2014,30(16):230-238.
[15]	SOUMARÉ M,DEMEYER A,TACK F M G,et al.Chemical characteristics of Malian and Belgian solid waste composts[J].Bioresource Technology,2002,81(2):97-101.
[16]	曹云,常志州,黄红英,等.畜禽粪便堆肥前期理化及微生物性状研究[J].农业环境科学学报,2015,34(11):2198-2207.
[17]	王友玲,邱慧珍,GHANNEY P,等.通风方式对牛粪堆肥氨气排放与氮素转化的影响[J].农业机械学报,2020,51(11):313-320.
[18]	石门.生物与生态[M].呼和浩特:远方出版社,2005.
[19]	赵秀玲,朱新萍,罗艳丽,等.温度与秸秆比例对牛粪好氧堆肥的影响[J].环境工程学报,2014,8(1):334-340.
[20]	KENG Z X,CHONG S,NG C G,et al.Community-scale composting for food waste:A life-cycle assessment-supported case study[J].Journal of Cleaner Production,2020,261:121220-121231.
[21]	王建才,朱荣生,刘兴华,等.微生物菌剂对猪粪堆肥中细菌群落结构的影响[J].应用生态学报,2020,31(7):2449-2456.
[22]	RIVAS M N,BURTON O T,WISE P,et al.A microbiota signature associated with experimental food allergy promotes allergic sensitization and anaphylaxis[J].The Journal of Allergy and Clinical Immunology,2013,131(1):201-212.
[23]	LIU Y W,FENG Y,CHENG D M,et al.Gentamicin degradation and changes in fungal diversity and physicochemical properties during composting of gentamicin production residue[J].Bioresource Technology,2017,244:905-912.
[24]	WANG R,ZHANG J Y,SUI Q W,et al.Effect of red mud addition on tetracycline and copper resistance genes and microbial community during the full scale swine manure composting[J].Bioresource Technology,2016,216:1049-1057.
[25]	ZHANG L,GU J,WANG X J,et al.Behavior of antibiotic resistance genes during co-composting of swine manure with Chinese medicinal herbal residues[J].Bioresource Technology,2017,244:252-260.
[26]	REN S T,LI A,GUO X Y,et al.Effects of co-composting of lincomycin mycelia dregs with furfural slag on lincomycin degradation,degradation products,antibiotic resistance genes and bacterial community[J].Bioresource Technology,2019,272:83-91.
[27]	杨萍萍,尹华,彭辉,等.外接菌种对污泥堆肥效能及堆体细菌群落的影响[J].环境科学,2017,38(8):536-3543.
[28]	史龙翔,谷洁,潘洪加,等.复合菌剂提高果树枝条堆肥过程中酶活性[J].农业工程学报,2015,31(5):244-251.
[29]	FRANKE-WHITTLE I H,KNAPP B A,FUCHS J,et al.Application of COMPOCHIP microarray to investigate the bacterial communities of different composts[J].Microbial Ecology,2009,57(3):510-521.
[30]	GANNES V,EUDOXIE G,HICKEY W J.Prokaryotic successions and diversity in composts as revealed by 454-pyrosequencing[J].Bioresource Technology,2013,133(2):573-580.
[31]	TAKAKU H,KODAIRA S,KIMOTO A,et al.Microbial communities in the garbage composting with rice hull as an amendment revealed by culture-dependent and-independent approaches[J].Journal of Bioscience and Bioengineering,2006,101(1):42-50.
[32]	XU S W,REUTER T,GILROYED B H,et al.Microbial communities and greenhouse gas emissions associated with the biodegradation of specified risk material in compost[J].Waste Management,2013,33(6):1372-1380.
[33]	ERMA W,AYUMI S,ROKIAH H,et al.Biodegradation of fibrillated oil palm trunk fiber by a novel thermophilic,anaerobic,xylanolytic bacterium Caldicoprobacter sp.CL-2 isolated from compost[J].Enzyme and Microbial Technology,2018,111:21-28.
[34]	EPHRAIM E,JACKSON M I,COCHRANE C,et al.Developmental changes in the canine gut microbiome during breastfeeding,weaning,socialization and maturation to adulthood[J].The FASEB Journal,2020,34(S1):1-1.
[35]	ZAINUDIN M H,MUSTAPHA N A,MAEDA T,et al.Biochar enhanced the nitrifying and denitrifying bacterial communities during the composting of poultry manure and rice straw[J].Waste Management,2020,106:240-249.
[36]	BENNUR T,KUMAR A R,ZINJARDE S,et al.Nocardiopsis species:incidence,ecological roles and adaptations[J].Microbiological Research,2015,174:33-47.
[37]	刘智萍,夏家帅,王晗,等.农业废弃物柑橘皮渣堆肥工艺优化及其微生物群落结构演替规律研究[J].三峡生态环境监测,2019,12(1):79-86.
[38]	SCOTTI R,MITCHELL A L,PANE C,et al.Microbiota characterization of agricultural green waste-based suppressive composts using omics and classic approaches[J].Agriculture,2020,10(3):61-90.
[39]	陆晓林,戴传超,贾永.堆肥过程中优势功能微生物菌群多样性及其影响因素[J].生态学杂志,2019,38(9):2866-2874.