污水厂二级出水中抗生素抗性细菌的紫外灭活与复活特性

李腾; 胡静; 宋海旺; 秦显祥; 程丽华; 毕学军

doi:10.13205/j.hjgc.202202003

污水厂二级出水中抗生素抗性细菌的紫外灭活与复活特性

doi: 10.13205/j.hjgc.202202003

李腾¹,
胡静¹,
宋海旺¹,
秦显祥¹,
程丽华^1,2, ,,
毕学军^1,2

1. 青岛理工大学环境与市政工程学院, 山东青岛 266033;
2. 城镇污水处理与资源化国家地方联合工程中心, 山东青岛 266033

基金项目:

山东省自然科学基金项目(ZR2020ME223)

国家重点研发计划项目(2017YFC0403404-003)

详细信息

作者简介:
李腾(1994-),男,硕士研究生,主要研究方向为污水处理与资源化。821362772@163.com

通讯作者:
程丽华(1973-),女,教授,主要研究方向为污水处理与资源化。chenglihua666@163.com

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出版历程
- 收稿日期: 2021-02-20
- 网络出版日期: 2022-04-02
- 刊出日期: 2022-04-02

INACTIVATION AND REACTIVATION OF ANTIBIOTIC-RESISTANT BACTERIA IN FILTERED SECONDARY EFFLUENT OF WASTEWATER TREATMENT PLANT DURING AND AFTER UV DISINFECTION

1. School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China;
2. State and Local Joint Engineering Center of Urban Sewage Treatment and Recycling, Qingdao 266033, China

摘要

摘要: 抗生素抗性细菌(ARB)作为新兴污染物受到了广泛关注,但紫外线(UV)消毒对ARB去除效果的研究尚不够充分。以某城市污水处理厂过滤后的二级出水为研究对象,通过分析UV消毒前后水中四环素、氨苄西林、氯霉素及链霉素抗性细菌丰度的变化,探究了UV消毒对ARB的去除作用及ARB的光复活与暗修复潜能。结果表明:UV对过滤后二级出水中的4种ARB具有一定灭活能力,4种ARB对UV消毒的耐受能力由高到低分别为:氨苄西林抗性细菌>四环素抗性细菌>链霉素抗性细菌≈氯霉素抗性细菌。当UV消毒剂量为20 mJ/cm²时,氯霉素与链霉素抗性细菌可被完全灭活,消毒后24 h内,这2种ARB未出现光复活或暗修复现象,这一剂量的UV不能完全灭活氨苄西林与四环素抗性细菌,且被灭活后的ARB可实现部分光复活及暗修复。当UV消毒剂量达到80 mJ/cm²时,这4种ARB均被全部灭活,消毒后6 h内,氨苄西林抗性细菌出现了复活现象。不论是在光照还是避光的条件下,UV消毒24 h后水中的总异养菌群中有超过70.32%的细菌对氨苄西林具有抗性,因此,单独UV消毒并不能有效地控制ARB从污水处理厂向环境中的传播。
- 紫外线消毒 /
- 总异养菌群 /
- 抗生素抗性细菌 /
- 光复活 /
- 暗修复
Abstract: Antibiotic resistant bacteria(ARB) have been widely concerned as an emerging contaminant, but the effect of ultraviolet(UV) disinfection on ARB removal has not been fully studied. This paper focused on the disinfection of the filtered secondary effluent from a municipal sewage treatment plant, investigated the removal effect of UV disinfection on ARB and the photoreactivation and dark repair potential of ARB by analyzing the abundance of bacteria with resistance to tetracycline, ampicillin, chloramphenicol or streptomycin before and after UV disinfection. The results showed that UV could partly inactivate the ARB, and the resistance ability of the four ARB to UV disinfection followed the order of ampicillin resistant bacteria>tetracycline resistant bacteria>streptomycin resistant bacteria≈chloramphenico resistant bacteria. UV disinfection with dosage of 20 mJ/cm² could completely inactivate chloramphenicol resistant bacteria and streptomycin resistant bacteria, and these two kinds of ARB did not appear photoreactivation or dark repair ability within 24 h post disinfection. This dosage of UV could not completely inactivate ampicillin resistant bacteria or tetracycline resistant bacteria, and the inactivated bacteria could partly reactivate with or without the light. When UV dosage reached 80 mJ/cm², these four kinds of ARB were totally inactivated. Ampicillin resistant bacteria showed the reactivation ability within 6 h post disinfection. Whether putting in light or in dark for 24 h after disinfection, more than 70.32% of the total heterotrophic bacteria in water sample disinfected by UV were resistant to ampicillin. Therefore, UV disinfection alone could not effectively control the spread of ARB from sewage treatment plants to the environment.
- UV disinfection /
- total heterotrophic bacteria /
- antibiotic resistant bacteria /
- photoreactivation /
- dark repair

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参考文献(21)

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