矿化垃圾层微生物群落结构及多样性分析

谢明德; 冯梅; 唐一鸣; 易小莹; 刘丹

doi:10.13205/j.hjgc.202202007

矿化垃圾层微生物群落结构及多样性分析

doi: 10.13205/j.hjgc.202202007

谢明德^1,2,
冯梅²,
唐一鸣²,
易小莹²,
刘丹^2, ,

1. 西南交通大学生命科学与工程学院, 成都 611756;
2. 西南交通大学地球科学与环境工程学院, 成都 611756

基金项目:

国家自然科学基金(41602241)

详细信息

作者简介:
谢明德(1981-),男,博士研究生,实验师,主要研究方向为城市垃圾处理理论与技术。78871003@qq.com

通讯作者:
刘丹(1957-),男,博士,教授,主要研究方向为城市垃圾处理理论与技术、工程水环境效应及其控制。liudan-swju@163.com.cn

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出版历程
- 收稿日期: 2020-11-19
- 网络出版日期: 2022-04-02
- 刊出日期: 2022-04-02

ANALYSIS ON MICROBIAL COMMUNITY STRUCTURES AND DIVERSITY IN AGED REFUSE

1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 611756, China;
2. Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu 611756, China

摘要

摘要: 矿化垃圾层含有的丰富微生物群落在稳定渗滤液理化性质中发挥重要作用。为深入理解矿化垃圾中微生物群落对渗滤液有机物的降解作用,采用16S rRNA基因高通量测序技术和分析方法,研究了不同点位矿化垃圾微生物群落结构和多样性。结果表明:矿化垃圾含有丰富的微生物群落,各点位的丰富度相近,但多样性差别较大;各点位均含有(相对丰度>1.0%)厚壁菌门(Firmicutes)、广古菌门(Euryarchaeota)、绿弯菌门(Chloroflexi)、拟杆菌门(Bacteroidetes)、互养菌门(Synergistetes)、放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、浮霉菌门(Planctomycetes)、热孢菌门(Thermotogae)和Atribacteria;厚壁菌门在K1、K2、K3和K4点位相对丰度最高,分别为39.10%、31.79%、47.09%和33.84%,为优势菌门;广古菌门在渗滤液有机负荷较高的K1、K2点位以及水力停留时间较长的K4点位相对丰度较高,而在中部K3点位的相对丰度较低。微生物群落结构和多样性与渗滤液负荷相关,在渗滤液负荷较高的矿化垃圾层,微生物群落多样性较高。
- 矿化垃圾 /
- 渗滤液 /
- 微生物群落 /
- 多样性
Abstract: The microbiota contained in the aged refuse plays an important role in the treatment of leachate. In order to understand the effect of microbial community on the degradation of organic matters in leachate, 16 S rRNA gene high-throughput sequencing technology and analysis method were used to study the microbial community structure and diversity of aged refuse at different sites. The results showed that the aged refuse contained rich microbial communities, and the richness of each site was similar, but the diversity was different; each site contained Firmicutes, Euryarchaeota, Chloroflexi, Bacteroidetes, Synergistetes, Actinobacteria, Proteobacteria, Planctomycetes, Thermotogae and Atribacteria(with relative abundance of 1.0% above); Firmicutes, the dominant phyla, had the highest relative abundance at K1, K2, K3 and K4, which were 39.10%, 31.79%, 47.09% and 33.84%, respectively; the relative abundance of Euryarchaeota was higher at K1 and K2 with higher organic load of leachate and at K4 with longer hydraulic retention time, while the relative abundance was lower at K3 in the middle layer. The structure and diversity of the microbial community were related to the leachate loading, and the diversity of the microbial community was higher in the aged refuse layer with higher leachate loading. The rich microbial community in the aged refuse layer played an important role in stabilizing the physical and chemical properties of the leachate.
- aged refuse /
- leachate /
- microbial community /
- diversity

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