矿化垃圾层微生物群落结构及多样性分析

谢明德; 冯梅; 唐一鸣; 易小莹; 刘丹

doi:10.13205/j.hjgc.202202007

矿化垃圾层微生物群落结构及多样性分析

doi: 10.13205/j.hjgc.202202007

谢明德^1,2,
冯梅²,
唐一鸣²,
易小莹²,
刘丹^2, ,

1. 西南交通大学生命科学与工程学院, 成都 611756;
2. 西南交通大学地球科学与环境工程学院, 成都 611756

基金项目:

国家自然科学基金(41602241)

详细信息

作者简介:
谢明德(1981-),男,博士研究生,实验师,主要研究方向为城市垃圾处理理论与技术。78871003@qq.com

通讯作者:
刘丹(1957-),男,博士,教授,主要研究方向为城市垃圾处理理论与技术、工程水环境效应及其控制。liudan-swju@163.com.cn

计量
- 文章访问数: 241
- HTML全文浏览量: 35
- PDF下载量: 15
- 被引次数: 0
出版历程
- 收稿日期: 2020-11-19
- 网络出版日期: 2022-04-02
- 刊出日期: 2022-04-02

ANALYSIS ON MICROBIAL COMMUNITY STRUCTURES AND DIVERSITY IN AGED REFUSE

1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 611756, China;
2. Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu 611756, China

摘要

摘要: 矿化垃圾层含有的丰富微生物群落在稳定渗滤液理化性质中发挥重要作用。为深入理解矿化垃圾中微生物群落对渗滤液有机物的降解作用,采用16S rRNA基因高通量测序技术和分析方法,研究了不同点位矿化垃圾微生物群落结构和多样性。结果表明:矿化垃圾含有丰富的微生物群落,各点位的丰富度相近,但多样性差别较大;各点位均含有(相对丰度>1.0%)厚壁菌门(Firmicutes)、广古菌门(Euryarchaeota)、绿弯菌门(Chloroflexi)、拟杆菌门(Bacteroidetes)、互养菌门(Synergistetes)、放线菌门(Actinobacteria)、变形菌门(Proteobacteria)、浮霉菌门(Planctomycetes)、热孢菌门(Thermotogae)和Atribacteria;厚壁菌门在K1、K2、K3和K4点位相对丰度最高,分别为39.10%、31.79%、47.09%和33.84%,为优势菌门;广古菌门在渗滤液有机负荷较高的K1、K2点位以及水力停留时间较长的K4点位相对丰度较高,而在中部K3点位的相对丰度较低。微生物群落结构和多样性与渗滤液负荷相关,在渗滤液负荷较高的矿化垃圾层,微生物群落多样性较高。
- 矿化垃圾 /
- 渗滤液 /
- 微生物群落 /
- 多样性
Abstract: The microbiota contained in the aged refuse plays an important role in the treatment of leachate. In order to understand the effect of microbial community on the degradation of organic matters in leachate, 16 S rRNA gene high-throughput sequencing technology and analysis method were used to study the microbial community structure and diversity of aged refuse at different sites. The results showed that the aged refuse contained rich microbial communities, and the richness of each site was similar, but the diversity was different; each site contained Firmicutes, Euryarchaeota, Chloroflexi, Bacteroidetes, Synergistetes, Actinobacteria, Proteobacteria, Planctomycetes, Thermotogae and Atribacteria(with relative abundance of 1.0% above); Firmicutes, the dominant phyla, had the highest relative abundance at K1, K2, K3 and K4, which were 39.10%, 31.79%, 47.09% and 33.84%, respectively; the relative abundance of Euryarchaeota was higher at K1 and K2 with higher organic load of leachate and at K4 with longer hydraulic retention time, while the relative abundance was lower at K3 in the middle layer. The structure and diversity of the microbial community were related to the leachate loading, and the diversity of the microbial community was higher in the aged refuse layer with higher leachate loading. The rich microbial community in the aged refuse layer played an important role in stabilizing the physical and chemical properties of the leachate.
- aged refuse /
- leachate /
- microbial community /
- diversity

HTML全文

参考文献(26)

[1]	FLEMING I R,ROWE R K.Laboratory studies of clogging of landfill leachate collection and drainage systems[J].Canadian Geotechnical Journal,2004,41:134-153.
[2]	VANGULCK J F,RITTMANN B E,Cooke A J.Predicting biogeochemical calcium precipitation in landfill leachate collection systems[J].Biodegradation,2003,14(5):331-346.
[3]	陈云敏,兰吉武,李育超,等.垃圾填埋场渗沥液水位壅高及工程控制[J].岩石力学与工程学报,2014,33(1):154-163.
[4]	REAGAN M,ROWE R K.Clogging of gravel drainage layers permeated with landfill leachate[J].Journal of Geotechnical and Geoenvironmental Engineering,2007,133 (8):1026-1039.
[5]	ROWE R K,CULLIMORE D R,ARMSTRONG M D.Mass loading and the rate of clogging due to municipal solid waste leachate[J].Canadian Geotechnical Journal,2000,37(2):355-370.
[6]	石磊,张全,牛冬杰,等.矿化垃圾反应床处理渗滤液的微生物学特性[J].同济大学学报(自然科学版),2007,35(8):1085-1089.
[7]	赵由才,柴晓利,牛冬杰.矿化垃圾基本特性研究[J].同济大学学报(自然科学版),2006,34(10):1360-1364.
[8]	边炳鑫,赵由才,周正,等.矿化垃圾生物反应床处理渗滤液技术[J].环境工程,2007,25(1):52-56.
[9]	张华,赵由才.生活垃圾填埋场中矿化垃圾的综合利用[J].山东建筑工程学院学报,2004,19(3):46-51.
[10]	何岩.矿化垃圾反应床处理填埋场渗滤液工艺优化及运行机制研究[D].上海:同济大学,2008.
[11]	谢明德,唐一鸣,冯梅,等.利用矿化垃圾层预防和控制渗滤液导排系统堵塞[J].环境化学,2021,40(2):614-623.
[12]	CHEN S F,ZHOU Y Q,CHEN Y R,et al.FASTP:an ultra-fast all-in-one FASTQ preprocessor[J].Bioinformatics,2018,34(17):i884-i890.[13] MAGOČ T, SALZBERG S L. FLASH:fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011,27(21):2957-2963.
[14]	EDGAR R C.UPARSE:highly accurate OTU sequences from microbial amplicon reads[J].Nat Methods,2013,10(10):996-998.
[15]	STACKEBRANDT E,GOEBEL B M.Taxonomic Note:a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology[J].International Journal of Systematic Bacteriology,1994,44(4):846-849.
[16]	WANG Q,GARRITY G M,TIEDJE J M,et al.Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J].Applied and Environmental Microbiology,2007,73(16):5261-5267.
[17]	SHU D T,HE Y,YUE H,et al.Metagenomic insights into the effects of volatile fatty acids on microbial community structures and functional genes in organotrophic anammox process[J].Bioresource Technology,2015,196:621-633.
[18]	赵诗惠,吕亮,蒋志云,等.ABR-MBR组合工艺短程硝化过程的微生物种群[J].中国环境科学,2018,38(2):566-573.
[19]	PAN F,XU A H,XIA D S,et al.Effects of octahedral molecular sieve on treatment performance,microbial metabolism,and microbial community in expanded granular sludge bed reactor[J].Water Research,2015,87:127-136.
[20]	王宝山,温成成,孙秦川,等.石油烃类污染对青藏高原北麓河地区冻区土壤微生物多样性的影响[J].环境工程学报,2018,12(10):2917-2928.
[21]	BAREITHER C A,WOLFE G L,MCMAHON K D,et al.Microbial diversity and dynamics during methane production from municipal solid waste[J].Waste Management,2013,33(10):1982-1992.
[22]	LEONG L,DENMAN S,HUGENHOLTZ P,et al.Amino acid and peptide utilization profiles of the fluoroacetate-degrading bacterium synergistetes strain MFA1 under varying conditions[J].Microbial Ecology,2016,71(2):494-504.
[23]	ANA I D,PAULA O,ADRIANA L,et al.Metagenomic analysis of bacterial communities from a nitrification-denitrification treatment of landfill leachates[J].CLEAN:Soil,Air,Water,2019,47(11):1-22.
[24]	JAENICKE S,ANDER C,BEKEL T,et al.Comparative and joint analysis of two metagenomic datasets from a biogas fermenter obtained by 454-pyrosequencing[J].PLoS One,2011,6(1):e14519.
[25]	闫冰,夏嵩,桂双林,等.厌氧氨氧化菌富集培养过程微生物群落结构及多样性[J].环境科学,2020,41(12):5535-5543.
[26]	BOVIO P,CABEZAS A,ETCHEBEHERE C.Preliminary analysis of Chloroflexi populations in full scale UASB methanogenic reactors[J].Journal of Applied Microbiology,2019,126(2):667-683.
[27]	孟晓山,张玉秀,隋倩雯,等.氨氮浓度对猪粪厌氧消化及产甲烷菌群结构的影响[J].环境工程学报,2018,12(8):2346-2356.