有机负荷对污泥胞外聚合物（EPS）分泌特性及信号分子释放差异的影响

李亚静; 王少坡; 刘璐; 贾丽媛

doi:10.13205/j.hjgc.202202008

有机负荷对污泥胞外聚合物（EPS）分泌特性及信号分子释放差异的影响

doi: 10.13205/j.hjgc.202202008

李亚静^1,2, ,,
王少坡^1,2,
刘璐^1,2,
贾丽媛³

1. 天津城建大学环境与市政工程学院, 天津 300384;
2. 天津市水质科学与技术重点实验室, 天津 300384;
3. 天津艾凯文水处理科技有限公司, 天津 300000

基金项目:

天津市教委科研项目(2016CJ09)

天津市自然科学基金重点项目(18JCZDJC10080)

国家自然科学基金项目(51678388)

详细信息

通讯作者:
李亚静(1979-),女,副教授,博士,主要从事污水资源化的研究。yajingli79@163.com

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- 文章访问数: 443
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- PDF下载量: 12
- 被引次数: 0
出版历程
- 收稿日期: 2021-03-19
- 网络出版日期: 2022-04-02
- 刊出日期: 2022-04-02

SECRETORY CHARACTERISTICS OF EPS AND THE SIGNAL MOLECULES RELEASE UNDER DIFFERENT ORGANIC LOADING

LI Yajing^{1,2
, ,},
WANG Shaopo^1,2,
LIU Lu^1,2,
JIA Liyuan³

1. School of Environmental and Municipal Engineering, Tianjin Chengjian University, Tianjin 300384, China;
2. Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin 300384, China;
3. Tianjin IKWEN Water Treatment Co., Ltd, Tianjin 300000, China

摘要

摘要: 考察了连续流A²/O系统在3个有机负荷[0.29,0.58,1.16 kg COD/(m³·d)]条件下,胞外聚合物(EPS)分泌特性及信号分子(AHLs)的释放差异。结果表明:TB-EPS是污泥系统中EPS主要成分,TB-EPS组分具有较高的分子量,且分子量分布更广。有机负荷对LB-EPS各组分的影响大于TB-EPS。随着有机负荷的增加,LB-EPS中多糖(PS)含量增加趋势较蛋白质(PN)更明显,使得高负荷下污泥系统具有较强的沉降性。不同有机负荷下系统中AHLs的含量和种类相差较大。C10-HSL、C12-HSL和C14-HSL均在污泥中占主导地位。AHLs与LB-EPS及其组分的相关性较弱,较多AHLs类型与TB-EPS存在显著或较强的相关性,为AHLs调节EPS的合成从而实现系统稳定运行提供了理论支撑。
- 有机负荷 /
- 沉降性 /
- 脱氮除磷 /
- 胞外聚合物 /
- 信号分子
Abstract: The secretion characteristics of extracellular polymers(EPS) and the release of acylated homoserine lactones(AHLs) were investigated under three organic loading conditions [0.29, 0.58, 1.16 kg COD/(m³·d)] in continuous flow A²/O system. The results showed that TB-EPS was the main component of EPS, which had higher molecular weight and wider molecular weight distribution. The effect of organic loading on the components of LB-EPS was greater than that of TB-EPS. The increasing trend of polysaccharide(PS) content in LB-EPS was more obvious than that of protein(PN) with the increase of organic loading, making the sludge system have stronger settling property under high organic loading. The content and species of AHLs under different organic loads were significantly different. C10-HSL, C12-HSL and C14-HSL were dominant in sludge system. The correlation between AHLs and LB-EPS was weak, and there were significant or strong correlations between many types of AHLs and TB-EPS, which provided theoretical support for AHLs regulating EPS synthesis to achieve stable operation of the system.
- organic loading /
- settleability /
- nitrogen and phosphorus removal /
- extracellular polymeric substance(EPS) /
- acylated homoserine lactones(AHLs)

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参考文献(24)

[1]	LI D,LV Y F,ZENG H P,et al.Long term operation of continuous-flow system with enhanced biological phosphorus removal granules at different COD loading[J].Bioresource Technology,2016,216:761-767.
[2]	LIANG Y H,LI D,ZENG H P,et al.Rapid start-up and microbial characteristics of partial nitrification granular sludge treating domestic sewage at room temperature[J].Bioresource Technology,2015,196:741-745.
[3]	YU S J,SUN P D,ZHENG W,et al.The effect of COD loading on the granule-based enhanced biological phosphorus removal system and the recoverability[J].Bioresource Technology,2014,171:80-87.
[4]	WANG X,CHEN Z,SHEN J,et al.The Effect of COD Loading on the granule-based enhanced biological phosphorus removal system and the recoverability[J].Bioresource Technology,2019,271:258-265.
[5]	RUSANOWSKA P,CYDZIK-KWIATKOWSKA A,SWIATCZAK P,et al.Changes in extracellular polymeric substances (EPS) content and composition in aerobic granule size-fractions during reactor cycles at different organic loads[J].Bioresource Technology,2019,272:188-193.
[6]	LI H,ZHANG J F,SHEN L,et al.Production of polyhydroxyalkanoates by activated sludge:correlation with extracellular polymeric substances and characteristics of activated sludge[J].Chemical Engineering Journal,2019,361:219-226.
[7]	谭煜,付丽亚,周鉴,等.胞外聚合物(EPS)对污水处理影响的研究进展[J/OL].环境工程技术学报,2020(网络首发).doi: 10.12153/j.issn.1674 -991X.20200178.
[8]	TANG X,LIU S T,ZHANG Z T,et al.Identification of the release and effects of ahls in anammox culture for bacteria communication[J].Chemical Engineering Journal,2015,273:184-191.
[9]	崔理慧万俊锋.AHLs介导群感效应在好氧颗粒污泥中的研究进展[J/OL].微生物学通报,2020(网络首发).doi: 10.13344/j.microbiol.china.200180.
[10]	刘莎莎,梁家豪,李晋,等.信号分子在好氧污泥颗粒化中的作用及其控制策略[J].工业水处理,2021,41(1):25-29.
[11]	LI D,LV Y F,ZENG H P,et al.Effect of sludge retention time on continuous-flow system with enhanced biological phosphorus removal granules at different COD loading[J].Bioresource Technology,2016,219:14-20.
[12]	KAPAGIANNIDIS A G,ZAFIRIADIS I,AIVASIDIS A.Effect of basic operating parameters on biological phosphorus removal in a continuous-flow anaerobic-anoxic activated sludge system[J].Bioprocess and Biosystems Engineering,2012,35(3):371-382.
[13]	李柱.低温A/O-SBR系统中聚磷菌的代谢特性及颗粒污泥的群感效应研究[D].天津:天津城建大学,2019.
[14]	国家环境保护总局.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002,105-107.
[15]	南国英,李士波,代学民,等.SOUR作为活性污泥法SBR反应器运行参数的研究进展[J].市政技术,2018,36(6):163-165.
[16]	LI Y J,WANG S P,LI Z,et al.Extraction method and structural and composition characteristics of extracellular polymeric substances in granular sludge from an enhanced biological phosphorus removal system[J].Desalination and Water Treatment,2020,185:41-50.
[17]	ZHOU J,ZHOU L X,HUANG H Z.Optimization of extracellular polymeric substance extraction method and its role in the dewaterability of sludge[J].Environmental Science,2013,34:2752-2757.
[18]	SHENG G P,YU H Q,LI X Y.Extracellular polymeric substances (EPS) of microbial aggregates in biological wastewater treatment systems:a review[J].Biotechnology & Applied Microbiology,2010,28(6):882-894.
[19]	温丹丹,袁林江,陈希,等.3种不同工艺切换下活性污泥菌群结构及代谢产物对污泥沉降性能的影响[J].环境科学,2018,10(39):4644-4651.
[20]	DING Z J,BOURVEN I,GUIBAUD G,et al.Role of extracellular polymeric substances (EPS) production in bioaggregation:application to wastewater treatment[J].Applied Microbiology and Biotechnology,2015,99(23):9883-9905.
[21]	WANG B B,PENG D C,HOU Y P,et al.The important implications of particulate substrate in determining the physicochemical characteristics of extracellular polymeric substances (EPS) in activated sludge[J].Water Research,2014,58:1-8.
[22]	SHEN Q X,GAO J,LIU J,et al.A new acyl-homoserine lactone molecule generated by Nitrobacter winogradskyi[J].Scientific Reports,2016,6:1-11.
[23]	张玉鹏.UASB中微生物群体行为及AHLs介导的感应调节机制[D].哈尔滨:哈尔滨工业大学,2019.
[24]	胡惠秩.常/低温下AHLs类群体感应信号分子对SBBR系统影响的研究[D].哈尔滨:哈尔滨工业大学,2017.