CHARACTERISTICS OF SIMULTANEOUS TREATMENT OF NITROGEN AND PHOSPHORUS IN PYRITE BIOFILTER AND ITS MICROBIAL COMMUNITY
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摘要: 以黄铁矿为生物滤池的主要填料,对含低浓度NO3--N和PO43--P废水进行同步脱氮除磷处理,探讨其启动过程中污染物转化特性及其功能微生物变化。结果表明:经过30 d的运行可实现生物滤池同步脱氮除磷,TN最大去除速率可达到70 g/(m3·d)。HRT控制在6 h以内,可避免NO2--N的大量产生。出水ρ(TP)可降至0.2~0.4 mg/L,HRT对滤池中磷的去除几乎未产生影响。SEM-EDX和磷形态分析表明,废水中磷主要是以铁磷化合物的形式去除。功能微生物群落分析表明,硫自反硝化过程的主要功能微生物为Thiobacillus(27.6%)、Sulfurimonas(11.8%)、Thiohalobacter(10.9%)。在有机碳源缺少条件下,该系统是同时从废水去除NO3--N和PO43--P的有效途径。Abstract: As the effluent discharge index of sewage treatment plant tends to be more strict, deep denitrification and dephosphorization of tail water gradually becomes an indispensable process. In this study, pyrite was used as the main filler of biofilter to conduct simultaneous denitrification and dephosphorization treatment of wastewater with low concentration of NO3--N and PO43--P, and to investigate the pollutant conversion characteristics and functional microbial changes during the start-up process. The results showed that the simultaneous denitrification and dephosphorization of the biofilter could be realized after 30-day operation, and its maximum total nitrogen removal rate reached 70 g/(m3·d). HRT could be controlled within 6 h to avoid mass production of NO2--N. HRT had little effect on gradual removal of phosphorus from the biofilter and the effluent total phosphorus concentration reduced to 0.2~0.4 mg/L. SEM-EDX and phosphorus morphology analysis showed that phosphorus was mainly removed in the form of iron and phosphorus compounds. Analysis of functional microbial community showed that Thiobacillus (27.6%), Sulfurimonas (11.8%), Thiohalobacter (10.9%) were the main functional microorganisms in the process of autotrophic denitrification. This system was a promising way to remove NO3--N and PO43--P simultaneously from wastewater lack of organic carbon sources.
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Key words:
- biofilter /
- pyrite /
- sulfur autotrophic denitrification /
- phosphorus removal /
- microbial community
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