填料优化反硝化脱硫工艺系统的生物强化效果研究

郜爽; 李智灵; 王爱杰; 黄聪

doi:10.13205/j.hjgc.202204005

填料优化反硝化脱硫工艺系统的生物强化效果研究

doi: 10.13205/j.hjgc.202204005

郜爽¹,
李智灵¹,
王爱杰¹,
黄聪^1,2, ,

1. 哈尔滨工业大学水资源与环境国家重点实验室, 哈尔滨 150090;
2. 中国科学院天津工业生物技术研究中心, 天津 300308

基金项目:

国家自然科学基金资助项目"有机废水碳氮硫共脱除系统微生物功能网络及调控机制"(51808166)

详细信息

作者简介:
郜爽(1990-),女,博士研究生,主要研究方向为污水资源化利用。localinna.1990@163.com

通讯作者:
黄聪(1985-),男,副研究员,主要研究方向为工业废水处理技术。huangc@tib.cas.cn

计量
- 文章访问数: 174
- HTML全文浏览量: 32
- PDF下载量: 13
- 被引次数: 0
出版历程
- 收稿日期: 2021-09-10
- 网络出版日期: 2022-07-06

PROMOTION OF BIO-AUGMENTATION EFFECT BY DENITRIFYING SULFIDE REMOVAL PROCESS WITH FILLER OPTIMIZED

1. State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology, Harbin 150090, China;
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China

摘要

摘要: 在反硝化脱硫工艺体系内投加填料,进一步促进了菌剂的生物强化效果,实现了核心功能菌群的优化调控,提升了反硝化脱硫工艺体系的效能。研究发现:填料系统将单质硫的生成效能提升到对照组的1.5倍左右。无生物强化时填料表面生物膜中优势菌属为Pseudomonas和Azoarcus;填料和菌剂共同作用,可以将单质硫生成效能提升到对照组的2倍,此时生物膜优势菌属为Pseudomonas和Arcobacter,生物强化促进了硫氧化功能基因的表达。投加Pseudomonas sp.gs1进行生物强化,提升了填料表面生物膜的抗冲击负荷能力,系统经过冲击后单质硫生成率可以迅速恢复。
- 填料 /
- 生物强化 /
- 反硝化脱硫工艺 /
- 复杂碳源 /
- 核心功能菌
Abstract: The promotion of bio-augmentation by filler in a denitrifying sulfide removal process was realized by adding the bacterial agent to regulate the core functional flora, and the efficiency of the denitrification and desulfurization process were improved. It was revealed that the application of filler increased the efficiency of elemental sulfur generation to about 1.5 times of the control group. The dominant bacteria in the biofilm on the filler surface were Pseudomonas and Azoarcus. The combined effect of filler and bacterial agent increased the efficiency of elemental sulfur generation to twice of the control group, and then the dominant bacteria in the biofilm on the filler after bio-augmentation were Pseudomonas and Arcobacter. Expression of sulfur oxidation functional genes was strengthened by bio-augmentation. The artificially added Pseudomonas sp.gs1 improved the impact load resistance of the biofilm on the surface of the filler, and the elemental sulfur generation rate was quickly restored after the impact.
- filler /
- bio-augmentation /
- denitrifying sulfide removal process /
- complex carbon source /
- core functional bacteria

HTML全文

参考文献(19)

[1]	WANG A J, DU D Z, REN N Q. A new process of simultaneous de-sulfurization and de-nitrification[J]. China Science and Technology Information, 2005, 9.
[2]	张若晨.自养-异养联合反硝化系统中功能菌群互作规律及代谢机制[D].哈尔滨:哈尔滨工业大学, 2019.
[3]	LIU C, HAN K, LEE D J, et al. Simultaneous biological removal of phenol, sulfide, and nitrate using expanded granular sludge bed reactor[J]. Applied Microbiology and Biotechnology, 2016, 100(9):4211-2417.
[4]	LIU C, XU J, LEE D J, et al. Denitrifying sulfide removal process on high-tetracycline wastewater[J]. Bioresource Technology, 2016, 205:254-257.
[5]	马晓丹.脱硫脱氮细菌的分离筛选及其生物强化效能研究[D].哈尔滨:哈尔滨工业大学, 2015.
[6]	HUANG C, LIU W Z, LI Z L, et al. High recycling efficiency and elemental sulfur purity achieved in a biofilm formed membrane filtration reactor[J]. Water Research, 2018, 130:1-12.
[7]	SONWANI R K, SWAIN G, GIRI B S, et al. A novel comparative study of modified carriers in moving bed biofilm reactor for the treatment of wastewater:process optimization and kinetic study[J]. Bioresource Technology, 2019, 281:335-342.
[8]	ZHAO Y K, HUANG C, MA X D, et al. Bioaugmentation with the sulfur oxidizing Thauera sp. HDD1 for shortening the startup time in the denitrifying sulfide removal process[J]. Bioresource Technology Reports, 2019, 7:100192.
[9]	HUANG S, YU D S, CHEN G H, et al. Realization of nitrite accumulation in a sulfide-driven autotrophic denitrification process:simultaneous nitrate and sulfur removal[J]. Chemosphere, 2021, 278:130413.
[10]	陈雪琪.废水脱硫脱氮工艺效能调控及生物强化策略研究[D].哈尔滨:哈尔滨工业大学, 2020.
[11]	LI R H, WEI D Y, WANG W, et al. Pyrrhotite-sulfur autotrophic denitrification for deep and efficient nitrate and phosphate removal:synergistic effects, secondary minerals and microbial community shifts[J]. Bioresource Technology, 2020, 308:123302.
[12]	HUANG C, LIU Q, LI Z L, et al. Relationship between functional bacteria in a denitrification desulfurization system under autotrophic, heterotrophic, and mixotrophic conditions[J]. Water Research, 2021, 188:116526.
[13]	CAI M H, LUO G, LI J, et al. Substrate competition and microbial function in sulfate-reducing internal circulation anaerobic reactor in the presence of nitrate[J]. Chemosphere, 2021, 280:130937.
[14]	HUANG C, LIU Q, CHEN X Q, et al. Bioaugmentation with Thiobacillus sp. H1 in an autotrophic denitrification desulfurization microbial reactor:microbial community changes and relationship[J]. Environmental Research, 2020, 189:109927.
[15]	ZHANG R C, XU X J, CHEN C, et al. Interactions of functional bacteria and their contributions to the performance in integrated autotrophic and heterotrophic denitrification[J]. Water Research, 2018, 143:355-366.
[16]	ZHANG J B, WU P X, HAO B, et al. Heterotrophic nitrification and aerobic denitrification by the bacterium Pseudomonas stutzeri YZN-001[J]. Bioresource Technology, 2011, 102(21):9866-9869.
[17]	OBEROI A S, HUANG H, KHANAL S K, et al. Electron distribution in sulfur-driven autotrophic denitrification under different electron donor and acceptor feeding schemes[J]. Chemical Engineering Journal, 2021, 404:126486.
[18]	BANIHASHEMI A, VAN DYKE M I, HUCK P M. Application of long amplicon propidium monoazide-PCR to assess the effects of temperature and background microbiota on pathogens in river water[J]. Journal of Water and Health, 2017, 15(3):418-428.
[19]	PISHGAR R, DOMINIC J A, SHENG Z, et al. Denitrification performance and microbial versatility in response to different selection pressures[J]. Bioresource Technology, 2019, 281:72-83.