1株金霉素降解菌的筛选及基于响应面法的降解条件优化

何汶珊; 张茹; 李思琦; 李文进; 宋哲华; 彭光; 沈鹏; 王晓慧

doi:10.13205/j.hjgc.202205008

1株金霉素降解菌的筛选及基于响应面法的降解条件优化

doi: 10.13205/j.hjgc.202205008

何汶珊¹,
张茹¹,
李思琦¹,
李文进²,
宋哲华²,
彭光³,
沈鹏⁴,
王晓慧^1,4, ,

1. 北京化工大学水处理环保材料工程技术研究中心, 北京 100029;
2. 北京京环新能环境科技有限公司, 北京 100012;
3. 驻马店华中正大有限公司, 河南驻马店 463023;
4. 中国环境科学研究院, 北京 100012

基金项目:

国家重点研发技术项目(2018YFC1801501)

详细信息

作者简介:
何汶珊(1998-),女,硕士研究生,主要研究方向为水污染控制理论与技术。wenshanhecc@163.com

通讯作者:
王晓慧(1980-),男,教授,博士,主要研究方向为水污染控制理论与技术。xhwang@mail.buct.edu.cn

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- 文章访问数: 151
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- 被引次数: 0
出版历程
- 收稿日期: 2021-09-01
- 网络出版日期: 2022-07-02

SCREENING OF A CHLORTETRACYCLINE-DEGRADING STRAIN AND ITS DEGRADATION CONDITIONS OPTIMIZATION USING RESPONSE SURFACE METHODOLOGY

1. Beijing Water Treatment Environmental Protection Material Engineering Technology Research Center, Beijing University of Chemical Technology, Beijing 100029, China;
2. BESG New-Energy Environmental Engineering Co., Ltd, Beijing 100012, China;
3. Zhumadian Huazhong Chia Tai Co., Ltd., Zhumadian 463023, China;
4. Chinese Research Academy of Environmental Sciences, Beijing 100012, China

摘要

摘要: 从河南驻马店金霉素制药厂活性污泥中分离、筛选出1株金霉素降解菌,经16SrDNA鉴定为芽孢杆菌属(Bacillus sp.),命名为JMS-B01。为进一步提高该菌株的金霉素降解率,对其进行降解条件优化。采用正交试验确定显著影响金霉素降解率的3个因素(温度、金霉素浓度、接种量),通过Box-Behnken试验设计及响应面法分析确定最佳降解条件。结果表明:拟合所得的预测值与实际值相关性良好,最佳降解条件为温度34.0℃、金霉素浓度102.5 mg/L、接种量2%(体积分数),在此条件下金霉素降解率可达到96.50%。其中,温度是影响菌株JMS-B01对金霉素降解率的最重要因素之一。
- 金霉素 /
- 生物降解 /
- Box-Behnken /
- 响应面法
Abstract: A chlortetracycline-degrading strain was isolated from the activated sludge of a chlortetracycline pharmaceutical factory in Zhumadian in Henan. Based on the analysis of its 16S rDNA gene sequence, the isolated strain named JMS-B01 was identified as a member of the genus Bacillus. To improve the chlortetracycline degradation rate, the degradation conditions were optimized. Firstly, three factors playing important roles in the chlortetracycline degradation rate were selected based on the orthogonal test. Then box-Behnken experimental design and response surface method were used to analyze the optimal degradation conditions. The fitting curve had a good correlation with the measured values. The optimal conditions were as follows:temperature of 34.0℃, chlortetracycline concentration of 102.5 mg/L, inoculation amount of 2%(V/V). Under the optimal conditions mentioned above, the chlortetracycline degradation rate reached 96.50%. Temperature was one of the most important factors affecting the chlortetracycline degradation rate of strain JMS-B01.
- chlortetracycline /
- biodegradation /
- box-Behnken /
- response surface methodology

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参考文献(28)

[1]	展海银,周启星.不同介质中四环素类抗生素的检出水平与污染扩散[J].生态学杂志,2020,39(12):4206-4215.
[2]	徐向月,马文瑾,安博宇,等.四环素类抗生素在环境中的风险评估研究进展[J].中国畜牧兽医,2020,47(3):948-957.
[3]	敖蒙蒙,魏健,陈忠林,等.四环素类抗生素环境行为及其生态毒性研究进展[J].环境工程技术学报,2021,11(2):314-324.
[4]	王晓娟,年夫照,夏运生,等.抗生素使用现状及其在生态环境系统的行为研究进展[J].中国土壤与肥料,2020(6):286-292.
[5]	ALBERT J.Strategic dynamics of antibiotic use and the evolution of antibiotic-resistant infections[J].International Journal of Industrial Organization,2021,77:102759.
[6]	展海银,周启星.环境中四环素类抗生素污染处理技术研究进展[J].环境工程技术学报,2021,11(3):571-581.
[7]	JJEMBA P K.Excretion and ecotoxicity of pharmaceutical and personal care products in the environment[J].Ecotoxicology and Environmental Safety,2006,63(1):113-130.
[8]	DAGHRIR R,DROGUI P J E C L.Tetracycline antibiotics in the environment:a review[J].Environmental Chemistry Letters,2013,11(3):209-227.
[9]	MA X,YANG Z R,XU T T,et al.Chlortetracycline alters microbiota of gut or feces in pigs and leads to accumulation and migration of antibiotic resistance genes[J].Science of the Total Environment,2021,796:148976.
[10]	刘鹏霄,王旭,冯玲.自然水环境中抗生素的污染现状、来源及危害研究进展[J].环境工程,2020,38(5):36-42.
[11]	杨晓芳,杨涛,王莹,等.四环素类抗生素污染现状及其环境行为研究进展[J].环境工程,2014,32(2):123-127.
[12]	秦小宁.改性凹凸棒土和膨润土对水中四环素类抗生素的吸附去除研究[D].兰州:兰州交通大学,2020.
[13]	南志江,蒋煜峰,毛欢欢,等.玉米秸秆生物炭对灰钙土吸附金霉素的影响[J].环境科学,2021,42(12):9.
[14]	安璐,肖鹏飞.H₂O₂改性活性炭活化过硫酸钠降解盐酸金霉素[J].水处理技术,2021,47(5):62-66.
[15]	安璐,肖鹏飞.活性炭活化过硫酸钠氧化降解盐酸金霉素的研究[J].现代化工,2020,40(8):103-106 ,12.
[16]	LEE C,JEONG S,JU M,et al.Fate of chlortetracycline antibiotics during anaerobic degradation of cattle manure[J].Journal of Hazardous Materials,2020,386:121894.
[17]	赵永斌.3种四环素类抗生素降解菌的筛选及降解特性的研究[D].晋中:山西农业大学,2015.
[18]	成洁,杜慧玲,张天宝,等.四环素类抗生素降解菌的分离与鉴定[J].核农学报,2017,31(5):884-888.
[19]	AYDIN E,SAHIN M,TASKAN E,et al.Chlortetracycline removal by using hydrogen based membrane biofilm reactor[J].Journal of Hazardous Materials,2016,320:88-95.
[20]	赵晨光,陈路鹏,黄祚建,等.猪粪中四环素类抗生素降解菌的筛选及其在堆肥中的应用研究[J].家畜生态学报,2020,41(9):53-58.
[21]	张惠艳.金霉素降解菌株特性及代谢产物研究[D].北京:北京理工大学,2015.
[22]	LIAO X B,ZOU R S,LI B X,et al.Biodegradation of chlortetracycline by acclimated microbiota[J].Process Safety and Environmental Protection,2017,109:11-17.
[23]	赵凤,沈忱思,柳建设.铜绿微囊藻强化降解金霉素及降解产物的毒性评估[J].分析试验室,2020,39(5):580-585.
[24]	ZHAO F,ZHANG D,XU C Y,et al.The enhanced degradation and detoxification of chlortetracycline by Chlamydomonas reinhardtii[J].Ecotoxicology and Environmental Safety,2020,196:110552.
[25]	张迪,厉圆,沈忱思,等.金霉素及其异构体降解产物对斜生栅藻的毒性效应研究[J].农业环境科学学报,2019,38(4):756-764.
[26]	李莉,张赛,何强,等.响应面法在试验设计与优化中的应用[J].实验室研究与探索,2015,34(8):41-45.
[27]	栾庆祥,赵杨,周欣,等.单因素试验结合响应面分析法优化杜仲最佳提取工艺[J].药物分析杂志,2013,33(5):859-865.
[28]	李文兵,毕江涛,惠治兵.一株纤维素降解菌的分离、鉴定及发酵优化[J].安徽农业大学学报,2021,48(3):458-466.