1株金霉素降解菌的筛选及基于响应面法的降解条件优化

何汶珊; 张茹; 李思琦; 李文进; 宋哲华; 彭光; 沈鹏; 王晓慧

doi:10.13205/j.hjgc.202205008

1株金霉素降解菌的筛选及基于响应面法的降解条件优化

doi: 10.13205/j.hjgc.202205008

何汶珊¹,
张茹¹,
李思琦¹,
李文进²,
宋哲华²,
彭光³,
沈鹏⁴,
王晓慧^1,4, ,

1. 北京化工大学水处理环保材料工程技术研究中心, 北京 100029;
2. 北京京环新能环境科技有限公司, 北京 100012;
3. 驻马店华中正大有限公司, 河南驻马店 463023;
4. 中国环境科学研究院, 北京 100012

基金项目:

国家重点研发技术项目(2018YFC1801501)

详细信息

作者简介:
何汶珊(1998-),女,硕士研究生,主要研究方向为水污染控制理论与技术。wenshanhecc@163.com

通讯作者:
王晓慧(1980-),男,教授,博士,主要研究方向为水污染控制理论与技术。xhwang@mail.buct.edu.cn

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- 文章访问数: 198
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- 被引次数: 0
出版历程
- 收稿日期: 2021-09-01
- 网络出版日期: 2022-07-02

SCREENING OF A CHLORTETRACYCLINE-DEGRADING STRAIN AND ITS DEGRADATION CONDITIONS OPTIMIZATION USING RESPONSE SURFACE METHODOLOGY

1. Beijing Water Treatment Environmental Protection Material Engineering Technology Research Center, Beijing University of Chemical Technology, Beijing 100029, China;
2. BESG New-Energy Environmental Engineering Co., Ltd, Beijing 100012, China;
3. Zhumadian Huazhong Chia Tai Co., Ltd., Zhumadian 463023, China;
4. Chinese Research Academy of Environmental Sciences, Beijing 100012, China

摘要

摘要: 从河南驻马店金霉素制药厂活性污泥中分离、筛选出1株金霉素降解菌,经16SrDNA鉴定为芽孢杆菌属(Bacillus sp.),命名为JMS-B01。为进一步提高该菌株的金霉素降解率,对其进行降解条件优化。采用正交试验确定显著影响金霉素降解率的3个因素(温度、金霉素浓度、接种量),通过Box-Behnken试验设计及响应面法分析确定最佳降解条件。结果表明:拟合所得的预测值与实际值相关性良好,最佳降解条件为温度34.0℃、金霉素浓度102.5 mg/L、接种量2%(体积分数),在此条件下金霉素降解率可达到96.50%。其中,温度是影响菌株JMS-B01对金霉素降解率的最重要因素之一。
- 金霉素 /
- 生物降解 /
- Box-Behnken /
- 响应面法
Abstract: A chlortetracycline-degrading strain was isolated from the activated sludge of a chlortetracycline pharmaceutical factory in Zhumadian in Henan. Based on the analysis of its 16S rDNA gene sequence, the isolated strain named JMS-B01 was identified as a member of the genus Bacillus. To improve the chlortetracycline degradation rate, the degradation conditions were optimized. Firstly, three factors playing important roles in the chlortetracycline degradation rate were selected based on the orthogonal test. Then box-Behnken experimental design and response surface method were used to analyze the optimal degradation conditions. The fitting curve had a good correlation with the measured values. The optimal conditions were as follows:temperature of 34.0℃, chlortetracycline concentration of 102.5 mg/L, inoculation amount of 2%(V/V). Under the optimal conditions mentioned above, the chlortetracycline degradation rate reached 96.50%. Temperature was one of the most important factors affecting the chlortetracycline degradation rate of strain JMS-B01.
- chlortetracycline /
- biodegradation /
- box-Behnken /
- response surface methodology

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