厌氧互营苯甲酸降解菌 <i>Sporotomaculum syntrophicum</i>对玉米秸秆沼气发酵的生物强化作用

朱心宇; 张洁; 孙晓娇; 陈光辉; 王晓霞; 张培玉; 邱艳玲

doi:10.13205/j.hjgc.202205011

厌氧互营苯甲酸降解菌 Sporotomaculum syntrophicum对玉米秸秆沼气发酵的生物强化作用

doi: 10.13205/j.hjgc.202205011

1. 青岛大学环境科学与工程学院, 山东青岛 266071;
2. 临沂科技职业学院, 山东临沂 276000

基金项目:

宁夏回族自治区重点研发计划(2020BBF03008)

国家自然科学基金项目(51878363)

山东省自然科学基金项目(ZR2019MEE028)

详细信息

作者简介:
朱心宇(1998-),女,硕士研究生,主要研究方向为环境微生物。zhuzhu981002@163.com

通讯作者:
邱艳玲(1972-),女,博士,教授,主要研究方向为厌氧生物处理。qiuyl@qibebt.ac.cn

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出版历程
- 收稿日期: 2021-09-04
- 网络出版日期: 2022-07-02

BIOAUGMENTATION OF CORN STALKS FERMENTATION BY ANAEROBIC BENZOATE-DEGRADING BACTERIUM SPOROTOMACULUM SYNTROPHICUM

1. School of Environmental Science and Engineering, Qingdao University, Qingdao 266071, China;
2. Linyi Vocational College of Science and Technology, Linyi 276000, China

摘要

摘要: 木质素的有效降解是提高秸秆等木质纤维素原料沼气发酵效率的有效途径。木质素很难被厌氧微生物分解利用,然而有研究表明木质素可以经由苯甲酸、苯酚等芳香族化合物被微生物分解利用。采用批次试验探究厌氧苯甲酸降解菌Sporotomaculum syntrophicum FB对秸秆沼气发酵的影响。强化菌株S.syntrophicum接种比例的结果表明,接种比例为5%、10%和20%时,甲烷产量分别为252.2,244.9,234.8 mL/g TS,比对照组提高了20.5%、17.0%和12.2%,TS去除率提高了2.9%、3.1%和3.4%。投加5%~20% S.syntrophicum后的玉米秸秆中纤维素、半纤维素、木质素的去除率,比对照组提高了12%~13%,3%~5%和38%~46%。进一步试验结果显示,添加S.syntrophicum使甲基纤维素、木聚糖和碱木质素(纤维素、半纤维素和木质素的模式物)的甲烷产量分别提高了15.7%、11.4%和7.8%。
- 生物强化 /
- 玉米秸秆 /
- 沼气发酵 /
- 木质素 /
- Sporotomaculum syntrophicum
Abstract: The degradation of lignin is an effective way to improve the biogas fermentation efficiency of lignocellulosic materials, such as straw. For anaerobic microorganisms, lignin is difficult to use, however, recent studies indicated that lignin can be decomposed and utilized by microorganisms through aromatic compounds such as benzoate and phenol. The effect of bioaugmentation with a benzoate-degrading bacterium Sporotomaculum syntrophicum strain FB on the anaerobic corn stalk fermentation was evaluated by batch experiments. The results showed that when the inoculation ratio of S. syntrophicum was 5%, 10%, and 20%, the methane yields were 252.2, 244.9, 234.8 mL/g TS respectively, increased by 20.5%, 17.0%, 12.2% comparing with the control. TS removal rates increased by 2.9%, 3.1%, and 3.4%, respectively. The removal rates of cellulose, hemicellulose and lignin increased by 12%~13%, 3%~5% and 38%~46%, respectively, with the addition of 5%~20% S. syntrophicum. The further experiment verified that the addition of S. syntrophicum could improve the methane yields of methyl cellulose, xylan, and alkali lignin(models for cellulose, hemicelluloses, and lignin, respectively) by 15.7%, 11.4%, 7.8%.
- bioaugmentation /
- corn stalk /
- anaerobic fermentation /
- lignin /
- Sporotomaculum syntrophicum

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