重金属Ni、Cr复合污染下李氏禾的根际环境特征

覃辉; 林华; 丁娜; 师恩泽; 甘淑萍

doi:10.13205/j.hjgc.202205016

重金属Ni、Cr复合污染下李氏禾的根际环境特征

doi: 10.13205/j.hjgc.202205016

覃辉^1,2,
林华^1,2, ,,
丁娜^1,2,
师恩泽^1,2,
甘淑萍^1,2

1. 桂林理工大学环境科学与工程学院, 广西桂林 541004;
2. 岩溶地区水污染控制与用水安全保障协同创新中心, 广西桂林 541004

基金项目:

国家自然科学基金面上项目(52170154)

广西八桂学者和特聘专家项目

国家自然科学基金面上项目(52070051)

广西自然科学基金项目(2020GXNSFAA297256)

广西科技项目(桂科AD19110156)

广西高等学校高水平创新团队及卓越学者计划项目(桂财教函[2018]319)

详细信息

作者简介:
覃辉(1997-),男,硕士研究生,主要研究方向为重金属污染植物修复技术。1209811033@qq.com

通讯作者:
林华(1984-),男,博士,副教授,主要研究方向为重金属污染防治。linhua5894@163.com

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- 被引次数: 0
出版历程
- 收稿日期: 2021-11-03
- 网络出版日期: 2022-07-02

CHARACTERIZATION OF RHIZOSPHERE ENVIRONMENT OF LEERSIA HEXANDRA SWARTZ UNDER CONTAMINATION OF Ni AND Cr

TAN Hui^1,2,
LIN Hua^{1,2
, ,},
DING Na^1,2,
SHI En-ze^1,2,
GAN Shu-ping^1,2

1. College of Environment Science and Engineering, Guilin University of Technology, Guilin 541004, China;
2. Collaborative Innovation Center for Water pollution Control and Water Safety Guarantee in Karst Area, Guilin 541004, China

摘要

摘要: 为了将李氏禾应用于Ni、Cr复合污染土壤修复领域,通过根箱实验,研究了Ni、Cr复合污染下李氏禾根际环境特征的变化。结果表明:在不同浓度的Ni、Cr处理下,李氏禾根际土壤的pH值均显著低于(P<0.01)非根际土壤与基质土壤,根际土壤的脲酶、酸性磷酸酶、酸性转化酶活性均显著高于(P<0.05)非根际土壤与基质土壤。随着Ni、Cr处理浓度升高,李氏禾的pH值、土壤酶活性均呈不同程度的下降,李氏禾的株高、生物量及富集系数也受到抑制。在相关性分析中,李氏禾的株高和生物量与土壤pH值、土壤酶活性呈显著正相关(P<0.01),土壤pH值与土壤酶活性同样呈显著正相关(P<0.01)。高通量测序结果表明:李氏禾根际土壤相对丰度最高的3种菌群为变形菌门(Proteobacteria)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes);相对丰度最高的3种菌属依次为Alicyclobacillus属、Bordetella属和Mucilaginibacter属;Hydrotalea属对李氏禾的生长及富集能力可能具有促进作用。李氏禾可耐受较高水平的Ni、Cr污染,其根际可能存在金属抗性细菌,是一种潜在的可用于Ni、Cr复合污染土壤修复的植物。
- 重金属污染 /
- 植物修复 /
- 根际环境 /
- 土壤酶活性 /
- 微生物群落
Abstract: To apply Leersia Hexandra Swartz to remediate Ni and Cr complex contaminated soil, root box experiments were used to investigate the changes in the rhizosphere environmental characteristics of Leersia Hexandra Swartz under Ni and Cr composite contamination. The results showed that:the pH of the rhizosphere soil of Leersia Hexandra Swartz was significantly lower(P<0.01) than that of the non-rhizosphere soil and the substrate soil under different concentrations of Ni and Cr; athe urease, acid phosphatase, and acid convertase activities of the inter-rhizosphere soil were significantly higher(P<0.05) than that of the non-rhizosphere soil and the substrate soil. As the concentration of Ni and Cr treatment increased, the pH and soil enzyme activity of Leersia Hexandra Swartz showed different degrees of decrease, and the plant height, biomass, and enrichment coefficient of Leersia Hexandra Swartz were also inhibited. In the correlation analysis, plant height and biomass of Leersia hexandra Swartz showed a significant positive correlation(P<0.01) with soil pH and soil enzyme activity, and soil pH showed the same significant positive correlation(P<0.01) with soil enzyme activity. High-throughput sequencing results showed that the three groups of bacteria with the highest relative abundance in the rhizosphere soil of Leersia Hexandra Swartz were Proteobacteria, Firmicutes and Bacteroidetes; the three genera with the highest relative abundance were Alicyclobacillus, Bordetella and Mucilaginibacter; Hydrotalea may have a facilitating effect on the growth and enrichment capacity of Leersia Hexandra Swartz. The results showed that Leersia Hexandra Swartz could tolerate higher levels of Ni and Cr contamination and that metal-resistant bacteria may be present in its inter-roots, making it a potential plant that can be used for remediation of Ni and Cr complex contaminated soils.
- heavy metal pollution /
- phytoremediation /
- rhizosphere environment /
- soil enzyme activity /
- microbial community

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