不同水动力条件下间歇微孔曝气对河道底质微生物群落的影响

潘沈阳; 张文龙; 李轶; 杨雪梅

doi:10.13205/j.hjgc.202208004

不同水动力条件下间歇微孔曝气对河道底质微生物群落的影响

doi: 10.13205/j.hjgc.202208004

河海大学环境学院浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098

基金项目:

江苏省自然科学基金项目(BK20201317)

国家重点研发计划项目(2019YFC0408300)

详细信息

作者简介:
潘沈阳(1997-),男,博士研究生,主要研究方向为水资源保护与生态修复。jsyzpsy@qq.com

通讯作者:
张文龙(1987-),男,教授,主要研究方向为水资源保护与生态修复。1223zhangwenlong@163.com

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出版历程
- 收稿日期: 2021-10-04
- 刊出日期: 2022-11-08

EFFECTS OF INTERMITTENT MICROPOROUS AERATION ON MICROBIAL COMMUNITIES IN URBAN RIVER SEDIMENTS UNDER DIFFERENT HYDRODYNAMIC CONDITIONS

Key Laboratory of Integrated Regulation and Resources Development on Shallow Lakes, Ministry of Education, College of Environment, Hohai University, Nanjing 210098, China

摘要

摘要: 微孔曝气技术被广泛运用于城市河道治理中,在间歇曝气过程中和曝气停止后微生物群落结构和功能的变化仍不明晰。采用室内模拟试验探究了曝气5 d再静置10 d后,不同水动力条件下水体污染物浓度变化过程以及底质微生物群落结构和功能的响应规律。结果表明:不同水动力条件下间歇曝气过程中和曝气停止后DO均能维持较高水平,但水体污染物浓度在曝气停止后短期内均出现显著性增加,但低流速(v=0.24 m/s)条件下污染物浓度上升最为缓慢。从微生物群落组成方面来看,该现象是由于曝气停止静置10 d后,与有机物代谢相关的氢噬菌属等菌属仅在低流速条件下可成为优势菌属,使得该流速水体中有机物更易降解,污染物浓度上升更为缓慢。从微生物群落功能方面来看,在曝气停止静置10 d后,富营养化水体中的能量代谢、辅酶和维生素代谢等代谢通路更多存在于静止和高流速(v=0.93 m/s)条件下的水体中,解释了这2种流速条件下污染物浓度更高的现象。该研究结果可为通过曝气手段持久性改善河道水质提供理论依据。
- 水动力条件 /
- 微孔曝气 /
- 水质改善 /
- 微生物群落结构和功能
Abstract: Microporous aeration technology is widely used in urban river management. The changes in the structure and function of the microbial community during the intermittent aeration process and after aeration stopped are still unclear. Indoor simulation experiments were used in this paper to investigate the changing process of pollutant concentration in water bodies under different hydrodynamic conditions, as well as the response law of the structure and function of the sedimentary microbial community from the beginning of aeration to 5 days after aeration, and then 10 days. The results showed that: the DO could be maintained at a high level under different hydrodynamic conditions, both during the intermittent aeration process and after the aeration stopped, but the water pollutant concentration rose significantly after aeration stopped, and the pollutant concentration rose the most slowly under the low flow rate condition (v=0.24 m/s). In terms of microbial community composition, this phenomenon resulted from the genus Hydrogenophaga and Novosphingobium, which were related to organic matter metabolism, became the dominant genus only under the condition of low flow rate after aeration stopped and stood still for 10 days, making the organic matter in the water body more prone to degradation, and the pollutant concentration rise more slowly. In terms of microbial community function, after aeration stopped for 10 days, metabolic pathways such as energy metabolism, coenzyme and vitamin metabolism, which were usually more common in eutrophic water bodies, were more present in water bodies under static and high flow rate (v=0.93 m/s). That explained the higher pollutant concentration under these two flow rates. The research results can provide a theoretical basis for persistently improving river water quality through intermittent microporous aeration.
- hydrodynamic conditions /
- intermittent microporous aeration /
- water quality improvement /
- microbial community and function

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参考文献(29)

[1]	ZHANG W L,LEI M T,LI Y,et al.Determination of vertical and horizontal assemblage drivers of bacterial community in a heavily polluted urban river[J].Water Research,2019,161:98-107.
[2]	HE H,YU Q J,LAI C C,et al.The treatment of black-odorous water using tower bipolar electro-flocculation including the removal of phosphorus,turbidity,sulfion,and oxygen enrichment[J].Frontiers of Environmental Science & Engineering,2021,15(2):18-30.
[3]	张文龙,杨雪梅,王浩岚,等.河流微孔曝气系统充氧性能优化试验[J].水资源保护,2022(3):154-160.
[4]	王硕,刘蕴思,李攀.微纳米气泡对小微水体中好氧微生物群落的影响[J].中国给水排水,2020,36(15):29-34.
[5]	李明泽,崔康平,郭志,等.组合生物技术对黑臭河道治理的中试研究[J].水处理技术,2021,47(6):122-125.
[6]	FENG Q,GE R,SUN Y Q,et al.Revealing hydrodynamic effects on flocculation performance and surface properties of sludge by comparing aeration and stirring systems via computational fluid dynamics aided calculation[J].Water Research,2020,172:115500.
[7]	陈燕,刘国华,范强,等.不同溶解氧条件下A/O系统的除碳脱氮效果和细菌群落结构变化[J].环境科学,2015,36(7):2610-2616.
[8]	李函珂,杨远坤,王彬,等.城市河流内源释放与微生物群落构效关系[J].环境科学与技术,2021,44(3):29-36.
[9]	LIU F F,WANG Z Y,WU B,et al.Cable bacteria extend the impacts of elevated dissolved oxygen into anoxic sediments[J].The ISME Journal,2021,15(5):1551-1563.
[10]	王海珊,邹平,戴犇,等.优化生物组合技术提高黑臭水体净化效能[J].环境工程,2021,39(1):24-32.
[11]	胡万超,赵琛,王巧娟,等.饮用水快速砂滤池优势微生物群落的代谢功能解析[J].环境科学,2019,40(8):3604-3611.
[12]	梁培瑜,王烜,马芳冰.水动力条件对水体富营养化的影响[J].湖泊科学,2013,25(4):455-462.
[13]	国家环境保护总局《水和废水监测分析方法》编委会.水和废水监测分析方法 [M].4版中国环境科学出版社,2002.
[14]	CAPORASO J G,LAUBER C L,WALTERS W A,et al.Ultra-high-throughput microbial community analysis on the Illumina HiSeq and MiSeq platforms[J].The ISME Journal,2012,6(8):1621-1624.
[15]	TAN X,YANG Y L,LIU Y W,et al.Enhanced simultaneous organics and nutrients removal in tidal flow constructed wetland using activated alumina as substrate treating domestic wastewater[J].Bioresource Technology,2019,280:441-446.
[16]	PARKS D H,TYSON G W,HUGENHOLTZ P,et al.STAMP:statistical analysis of taxonomic and functional profiles[J].Bioinformatics,2014,30(21):3123-3124.
[17]	李大鹏.底泥再悬浮对磷的吸附和不同形态磷转化的影响研究[D].哈尔滨:哈尔滨工业大学,2008.
[18]	POLROT A,KIRBY J R,BIRKETT J W,et al.Combining sediment management and bioremediation in muddy ports and harbours:a review[J].Environmental Pollution,2021,289:117853.
[19]	ALBERDI A,GILBERT M T P.A guide to the application of Hill numbers to DNA-based diversity analyses[J].Molecular Ecology Resources,2019,19(4):804-817.
[20]	CHAO A,CHIU C H,JOST L.Unifying species diversity,phylogenetic diversity,functional diversity,and related similarity and differentiation measures through hill numbers[J].Annual Review of Ecology,Evolution,and Systematics,2014,45(1):297-324.
[21]	NIU T H,ZHOU Z,SHEN X L,et al.Effects of dissolved oxygen on performance and microbial community structure in a micro-aerobic hydrolysis sludge in situ reduction process[J].Water Research,2016,90:369-377.
[22]	GÜLAY A,MUSOVIC S,ALBRECHTSEN H J,et al.Ecological patterns,diversity and core taxa of microbial communities in groundwater-fed rapid gravity filters[J].The ISME Journal,2016,10(9):2209-2222.
[23]	HANADA S,PIERSON B K:The Family Chloroflexaceae[M].Dworkin M,Falkow S,Rosenberg E,et al.editor.New York:Springer,2006:815-842.
[24]	鲜文东,张潇橦,李文均.绿弯菌的研究现状及展望[J].微生物学报,2020,60(9):1801-1820.
[25]	王光华,刘俊杰,于镇华,等.土壤酸杆菌门细菌生态学研究进展[J].生物技术通报,2016,32(2):14-20.
[26]	AHMAD M,WANG P,LI J L,et al.Impacts of bio-stimulants on pyrene degradation,prokaryotic community compositions,and functions[J].Environmental Pollution,2021,289:117863.
[27]	ZHANG L,LI X C,FANG W K,et al.Impact of different types of anthropogenic pollution on bacterial community and metabolic genes in urban river sediments[J].Science of the Total Environment,2021,793:148475.
[28]	SHEN M Y,LI Q,REN M L,et al.Trophic status is associated with community structure and metabolic potential of planktonic microbiota in Plateau Lakes[J].Frontiers in Microbiology,2019,10:2560.
[29]	LI Q,LIN F B,YANG C,et al.A large-scale comparative metagenomic study reveals the functional interactions in six bloom-forming Microcystis-epibiont communities[J].Frontiers in Microbiology,2018,9:746.