RAPID START-UP AND STABILITY OF PARTIAL NITRIFICATION FOR DOMESTIC SEWAGE
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摘要: 以低碳氮比的生活污水为研究对象,采用SBR反应器,通过减少好氧阶段的搅拌时间快速启动短程硝化脱氮过程,对典型运行周期内氮去除规律进行研究,并从微生物角度进一步验证了短程硝化脱氮工艺的实现。结果表明:减少50%好氧搅拌时间后,亚硝酸盐积累率(NAR)由36.05%增加到54.06%,好氧阶段停止搅拌后,NAR被提高到90.17%,并且以此状态持续稳定运行;典型运行周期内SBR具有良好的NH4+-N去除效果和较高的NAR,实测NH4+-N去除率达89.46%,出水NAR达89.13%;实时荧光定量PCR技术(q-PCR)检测表明,经过140 d的种群优化,污泥中氨氧化菌(AOB)和亚硝酸盐氧化菌(NOB)含量分别占总菌数的70.3%和2.1%,从分子生物学角度验证了短程硝化工艺的实现。Abstract: In this paper, domestic sewage with low carbon to nitrogen ratio was taken as the research object, and the SBR reactor was used to quickly start the partial nitrification by reducing the stirring time in aerobic stage. The nitrogen removal law of typical operating cycles was studied, and the realization of partial nitrification was further verified from the perspective of microorganisms. After reducing the aerobic stirring time by 50%, the nitrite accumulation rate (NAR) increased from 36.05% to 54.06%. After stopping aerobic stirring, the NAR increased to 90.17%, and it continued to operate stably in this state. In a typical operation cycle, SBR had a good ammonia nitrogen removal effect and high NAR. The removal rate of NH4+-N reached 89.46% and the NAR of effluent reached 89.13%. The detection result of quantitative real-time PCR (q-PCR) showed that after 140 days of population optimization, the contents of ammonia oxidizing bacteria (AOB) and nitrite oxidizing bacteria (NOB) in the sludge accounted for 70.3% and 2.1% of the total bacteria, respectively. The realization of partial nitrification was verified from the perspective of molecular biology.
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Key words:
- domestic sewage /
- SBR /
- partial nitrification /
- rapid start-up /
- microbial community
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[1] 牛晓倩,周胜虎,邓禹.脱氮微生物及脱氮工艺研究进展[J].生物工程学报,2021,37(10):3505-3519. [2] SUN H W, PENG Y Z, WANG S Y, et al. Achieving nitritation at low temperatures using free ammonia inhibition on Nitrobacter and real-time control in an SBR treating landfill leachate[J]. Journal of Environmental Sciences,2015,30:157-163. [3] MIAO Y Y, ZHANG L, YANG Y D, et al. Start-up of single-stage partial nitrification-anammox process treating low-strength swage and its restoration from nitrate accumulation[J]. Bioresource Technology,2016,218:771-779. [4] 周少奇,周吉林.生物脱氮新技术研究进展[J].环境污染治理技术与设备,2000, 1(6):11-19. [5] ZHANG Z Z, ZHANG Y, CHEN Y G. Recent advances in partial denitrification in biological nitrogen removal:from enrichment to application[J]. Bioresource Technology, 2019,298:122444. [6] VOETS P, VANSTAEN H, VERTRAETE W. Removal of nitrogen from highly nitrogenous wastewaters[J]. Journal of the Water Pollution Control Federation,1975,47:394-398. [7] HELLINGA C, SCHELLEN A A J C, MULDER J W, et al. The sharon process:an innovative method for nitrogen removal from ammonium-rich waste water[J]. Water Science & Technology, 1998, 37(9):135-142. [8] 吴军,张悦,徐婷,等.AOB溶解氧亲和力低于NOB条件下序批反应器中NOB淘汰的实现机制[J].中国环境科学,2016,36(12):3583-3590. [9] 夏俊方,王树涛,张永明,等.短程硝化反应中污染物降解动力学及微生物群落研究[J].哈尔滨工业大学学报,2018,50(2):65-70. [10] 孙迎雪,徐栋,田媛,等. 短程硝化-反硝化生物滤池脱氮机制研究[J]. 环境科学, 2012,33(10):3501-3506. [11] 刘诚诚,于德爽,陈光辉,等.盐度和曝气时间对包埋颗粒短程硝化启动的影响及其动力学分析[J].环境工程学报,2020,14(1):68-76. [12] EYICE O, INCE O, INCE B K. Monitoring the abundance and the activity of ammonia-oxidizing bacteria in a full-scale nitrifying activated sludge reactor[J]. Environmental Science and Pollution Research,2015, 22(3):2328-2334. [13] ZHANG L, ZHANG S J, GAN Y P, et al. Bio-augmentation to rapid realize partial nitrification of real sewage[J]. Chemosphere, 2012, 88(9):1097-1102. [14] BLACKBURNE R, YUAN Z G, KELLER J. Demonstration of nitrogen removal via nitrite in a sequencing batch reactor treating domestic wastewater[J]. Water Research,2008,42(8/9):2166-2176. [15] 赵晴,周浩,吕慧,等.AO-SBR短程硝化反硝化垃圾渗滤液预处理中试应用[J].环境工程学报,2021,15(2):545-552. [16] 呼晓明,陈英文,严伟峰,等.生物流化床短程硝化的快速启动及影响因素研究[J].环境科学与技术,2012,35(7):16-20. [17] 李柏林,杨丹丹,黄馨,等.基于DO和游离氨联合控制的短程硝化快速启动及稳定运行研究[J].环境污染与防治,2018,40(11):1219-1223. [18] 温婧玉,成浩楠,李竹君,等.短程硝化的快速启动调控研究[J].水处理技术,2019,45(8):125-129,132. [19] 金兆丰, 徐竟成. 城市污水回用技术手册[M]. 北京:化学工业出版社, 2004. [20] ZHANG Y, LI M Q, DONG L, et al. Effects of biochar dosage on treatment performance, enzyme activity and microbial community in aerated constructed wetlands for treating low C/N domestic sewage[J]. Environmental Technology & Innovation, 2021,24:101919. [21] LU S B, GAO X R, WU P T, et al. Assessment of the treatment of domestic sewage by a vertical-flow artificial wetland at different operating water levels[J]. Journal of Cleaner Production, 2019, 208:649-655. [22] 刘莉莉, 高大文, 张明慧. 分置式厌氧陶瓷膜生物反应器处理模拟生活污水试验研究[J]. 环境科学学报, 2018, 38(11):4259-4265. [23] 王龙涛. 秋季潮汐流模拟湿地对生活污水的处理效果[J]. 环境工程学报, 2016,10(10):5352-5358. [24] 国家环境保护总局.水和废水监测分析方法[M].4版. 北京:中国环境科学出版社,2002. [25] FORD D L, KACHTICK J W. Comprehensive analysis of nitrification of chemical processing wastewaters[J]. Journal-Water Pollution Control Federation, 1980,52(11):2726-2746. [26] CHEN Z, LUO X Q, HU R G, et al. Impact of long-term fertilization on the composition of denitrifier communities based on nitrite reductase analyses in a paddy soil[J]. Microbial Ecology, 2010,60(4):850-861. [27] ZHANG X W, HU Z, NGO H H, et al. Simultaneous improvement of waste gas purification and nitrogen removal using a novel aerated vertical flow constructed wetland[J]. Water Research,2018,130:79-87. [28] AMANN R I. In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes[M]//Molecular Microbial Ecology Manual Netherland:Kluwer Academic Publishers, 1995:1-15. [29] 曾薇,张悦,李磊,等.生活污水常温处理系统中AOB与NOB竞争优势的调控[J]. 环境科学,2009,30(5):1430-1436. [30] 卞伟,李军,赵白航,等.硝化污泥中AOB/NOB对硝化特性的影响[J].中国环境科学,2016,36(8):2395-2401. [31] GE S J, WANG S Y, YANG X, et al. Detection of nitrifiers and evaluation of partial nitrification for wastewater treatment:a review[J]. Chemosphere, 2015, 140:85-98. [32] RUIZ G, JEISON D, CHAMY R. Nitrification with high nitrite accumulation for the treatment of wastewater with high ammonia concentration[J]. Water Research, 2003, 37(6):1371-1377. [33] 徐婷,王丽,吴军.不同pH条件下短程硝化序批实验和数学模拟[J].环境工程学报,2016,10(6):2840-2846. [34] 李娜,胡筱敏,李国德,等.MBBR中HRT与pH对短程硝化反硝化的影响[J].工业水处理,2016,36(10):20-23. [35] 彭永臻,李璐凯,李夕耀,等. 不同pH值及碱性物质对短程硝化的影响[J]. 北京工业大学学报, 2017, 43(10):1554-1562. [36] 王桃,邵兆伟,刘安迪,等.交替曝气对短程硝化启动及脱氮特性的影响[J].环境工程学报,2020,14(12):3399-3407. [37] 袁忠玲,秦彦荣,张民安,等.曝气参数对短程硝化的影响及氮素等高线分析[J].中国环境科学,2021,41(2):650-659. [38] 杨庆,杨玉兵,杨忠启,等.溶解氧对短程硝化稳定性及功能菌群的影响[J].中国环境科学,2018,38(9):3328-3334. [39] 吴朕君,穆剑楠,单润涛,等.基于DO和ORP的短程硝化SBR控制方法研究[J].水处理技术,2019,45(7):114-118,139. [40] 罗珊,李新,钟铭,等.低碳氮比生活污水短程硝化反硝化的快速启动研究[J].环境科学与技术,2020,43(7):28-34. [41] 张凯,李军,梁东博,等.IFAS工艺短程硝化过程中功能菌的动力学特性[J].中国环境科学,2020,40(4):1507-1514. [42] 卢欣欣,王怡,黄瑞雪.MBBR一体式耦合短程硝化-厌氧氨氧化处理污泥水[J].环境工程学报,2020,14(7):1827-1833.
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