厌氧消化对污泥中抗生素抗性基因削减的影响

向银萍; 熊炜平; 张燕茹; 贾美莹; 彭海豪; 杨朝晖

doi:10.13205/j.hjgc.202210026

厌氧消化对污泥中抗生素抗性基因削减的影响

doi: 10.13205/j.hjgc.202210026

向银萍^1,2,
熊炜平^1,2,
张燕茹³,
贾美莹^1,2,
彭海豪^1,2,
杨朝晖^1,2, ,

1. 湖南大学环境科学与工程学院, 长沙 410082;
2. 环境生物与控制教育部重点实验室(湖南大学), 长沙 410082;
3. 湖南省林业科学院省部共建木本油料资源利用国家重点实验室, 长沙 410004

基金项目:

湖南省创新型省份建设专项资金(2021SK2040)

湖南省科技创新计划(2021RC2100)

国家自然科学基金(51848258)

详细信息

作者简介:
向银萍(1995-),女,博士,主要研究方向为固体废物处理与资源化。echoxiang@hnu.edu.cn

通讯作者:
杨朝晖(1963-),男,教授,主要研究方向为固体废物处理与资源化、环境生物技术、水污染控制工程。yzh@hnu.edu.cn

计量
- 文章访问数: 322
- HTML全文浏览量: 46
- PDF下载量: 8
- 被引次数: 0
出版历程
- 收稿日期: 2022-01-03

EFFECT OF SLUDGE ANAEROBIC DIGESTION ON THE REDUCTION OF ANTIBIOTIC RESISTANCE GENES

1. College of Environmental Science and Engineering, Hunan University, Changsha 410082, China;
2. Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, China;
3. State Key Laboratory of Utilization of Woody Oil Resource, Hunan Academy of Forestry, Changsha 410004, China

摘要

摘要: 为了系统地了解厌氧消化及其强化工艺对污泥中抗生素抗性基因的削减效果,明确抗性基因在厌氧消化过程中的降解机制,从几种常见的厌氧消化工艺入手,结合国内外研究进展,综述了高温厌氧消化、预处理和投加外源添加剂等强化工艺对污泥中不同抗生素抗性基因的削减效果和影响因素,发现污泥中抗生素抗性基因的归趋主要与污泥中选择性压力的大小、宿主菌群的丰度、水平转移的效率和抗性基因的耐药机制等有关。厌氧消化及其强化工艺主要通过破坏污泥细胞结构,减少宿主菌群丰度或降低抗性基因水平转移风险的作用机制来实现抗生素抗性基因的高效削减。
- 污泥 /
- 抗生素抗性基因 /
- 厌氧消化 /
- 宿主菌群 /
- 水平基因转移
Abstract: In order to systematically understand the removal efficiency of antibiotic resistance genes (ARGs) in sludge during anaerobic digestion and clarify the degradation mechanism of antibiotic resistance genes, this paper reviewed the effects of several common anaerobic digestion intensification processes, such as thermophilic anaerobic digestion, pretreatment technologies and additives injection on ARGs removal, taking the global research progress into account. It was found that the fate of ARGs was mainly related to the selective pressure in the sludge, the abundance of the host bacteria, the efficiency of horizontal gene transfer and the resistance mechanism of ARGs. Anaerobic digestion and its intensification processes mainly achieved an efficient reduction of ARGs by destroying the sludge cell structure, reducing the abundance of host bacteria and reducing the risk of horizontal gene transfer.
- sludge /
- antibiotic resistance genes /
- anaerobic digestion /
- host bacteria /
- horizontal gene transfer

HTML全文

参考文献(71)

[1]	戴晓虎. 我国污泥处理处置现状及发展趋势[J]. 科学(上海), 2020, 72(6):30-34.
[2]	XU Y, LU Y Q, ZHENG L K, et al. Perspective on enhancing the anaerobic digestion of waste activated sludge[J]. Journal of Hazardous Materials, 2020, 389:121847.
[3]	SCHUELER J, LANSING S, CROSSETTE E, et al. Tetracycline, sulfadimethoxine, and antibiotic resistance genedynamics during anaerobic digestion of dairy manure[J]. Journal of Environmental Quality, 2021, 50:694-702.
[4]	LUO J Y, ZHANG Q, ZHA J N, et al. Potential influences of exogenous pollutants occurred in waste activated sludge on anaerobic digestion:a review[J]. Journal of Hazardous Materials, 2020, 383:121176.
[5]	AMHA Y M, SINHA P, LAGMAN J, et al. Elucidating microbial community adaptation to anaerobic co-digestion of fats, oils, and grease and food waste[J]. Water Research, 2017, 123:277-289.
[6]	REN S, USMAN M, TSANG D C W, et al. Hydrochar-facilitated anaerobic digestion:evidence for direct interspecies electron transfer mediated through surface oxygen-containing functional groups[J]. Environmental Science & Technology, 2020, 54(9):5755-5766.
[7]	WANG Z Y, LIU T, DUAN H R, et al. Post-treatment options for anaerobically digested sludge:current status and future prospect[J]. Water Research, 2021, 205:117665.
[8]	XU R, YANG Z H, ZHENG Y, et al. Metagenomic analysis reveals the effects of long-term antibiotic pressure on sludge anaerobic digestion and antimicrobial resistance risk[J]. Bioresource Technology, 2019, 282:179-188.
[9]	TIAN Z, LIU R Y, ZHANG H, et al. Developmental dynamics of antibiotic resistome in aerobic biofilm microbiota treating wastewater under stepwise increasing tigecycline concentrations[J]. Environment International, 2019, 131:105008.
[10]	ZHANG J Y, WANG Z Y, WANG Y W, et al. Effects of graphene oxide on the performance, microbial community dynamics and antibiotic resistance genes reduction during anaerobic digestion of swine manure[J]. Bioresource Technology, 2017, 245:850-859.
[11]	LUO G, LI B, LI L G, et al. Antibiotic resistance genes and correlations with microbial community and metal resistance genes in full-scale biogas reactors as revealed by metagenomic analysis[J]. Environmental Science & Technology, 2017, 51(7):4069-4080.
[12]	WANG P L, WU D, SU Y L, et al. Dissemination of antibiotic resistance under antibiotics pressure during anaerobic co-digestion of food waste and sludge:insights of driving factors, genetic expression, and regulation mechanism[J]. Bioresource Technology, 2021, 344:126257.
[13]	KLEIN E Y, BOECKEL T P V, Martinez E M, et al. Global increase and geographic convergence in antibiotic consumption between 2000 and 2015[J]. Proceedings of the National Academy of Sciences, USA, 2018, 115:3463-3470.
[14]	ZHANG Q Q, YING G G, PAN C G, et al. Comprehensive evaluation of antibiotics emission and fate in the river basins of China:source analysis, multimedia modeling, and linkage to bacterial resistance[J]. Environmental Science & Technology, 2015, 49(11):6772-6782.
[15]	O'NEILL J. Antimicrobial resistance:tackling a crisis for the future health and wealth of nations[J]. 2014.
[16]	SU J Q, WEI B, OU-YANG W Y, et al. Antibiotic resistome and its association with bacterial communities during sewage sludge composting[J]. Environmental Science & Technology, 2015, 49(12):7356-7363.
[17]	ZHOU Z C, FENG W Q, HAN Y, et al. Prevalence and transmission of antibiotic resistance and microbiota between humans and water environments[J]. Environment International, 2018, 121:1155-1161.
[18]	YING Y, LI B, JU F, et al. Exploring variation of antibiotic resistance genes in activated sludge over a four-year period through a metagenomic approach[J]. Environmental Science & Technology, 2013, 47(18):10197-10205.
[19]	姚鹏城, 陈嘉瑜, 张永明, 等. 废水处理系统中抗生素抗性基因分布特征[J]. 环境科学, 2019, 40(11):5024-5031.
[20]	ZHANG Y, LI A L, DAI T J, et al. Cell-free DNA:a neglected source for antibiotic resistance genes spreading from WWTPs[J]. Environmental Science & Technology, 2018, 52(1):248-257.
[21]	张治国, 李斌绪, 李娜, 等. 污水深度处理工艺对抗生素抗性菌和抗性基因去除研究进展[J]. 农业环境科学学报, 2018, 37(10):2091-2100.
[22]	YANG Y, LI B, ZOU S C, et al. Fate of antibiotic resistance genes in sewage treatment plant revealed by metagenomic approach[J]. Water Research, 2014, 62:97-106.
[23]	AN X L, SU J Q, LI B, et al. Tracking antibiotic resistome during wastewater treatment using high throughput quantitative PCR[J]. Environment International, 2018, 117:146-153.
[24]	ZHANG Y R, YANG Z H, XIANG Y P, et al. Evolutions of antibiotic resistance genes (ARGs), class 1 integron-integrase (intI1) and potential hosts of ARGs during sludge anaerobic digestion with the iron nanoparticles addition[J]. Science of the Total Environment, 2020, 724:138248.
[25]	RAMSDEN S G S J, LAPARA T M. The role of anaerobic digestion in controlling the release of tetracycline resistance genes and class 1 integrons from municipal wastewater treatment plants[J]. Applied Microbiology and Biotechnology, 2009, 84:791-796.
[26]	JU F, LI B, MA L P, et al. Antibiotic resistance genes and human bacterial pathogens:co-occurrence, removal, and enrichment in municipal sewage sludge digesters[J]. Water Research, 2016, 91:1-10.
[27]	ZHANG K Y, GU J, WANG X J, et al. Analysis for microbial denitrification and antibiotic resistance during anaerobic digestion of cattle manure containing antibiotic[J]. Bioresource Technology, 2019, 291:121803.
[28]	XU R, YANG Z H, WANG Q P, et al. Rapid startup of thermophilic anaerobic digester to remove tetracycline and sulfonamides resistance genes from sewage sludge[J]. Science of the Total Environment, 2017, 612(9):788-798.
[29]	SUN W, QIAN X, GU J, et al. Mechanism and Effect of Temperature on Variations in Antibiotic Resistance Genes during Anaerobic Digestion of Dairy Manure[J]. Scientific Reports, 2016, 6:30237.
[30]	TIAN Z, ZHANG Y, YU B, et al. Changes of resistome, mobilome and potential hosts of antibiotic resistance genes during the transformation of anaerobic digestion from mesophilic to thermophilic[J]. Water Research, 2016, 98:261-269.
[31]	WU Y, CUI E P, ZUO Y R, et al. Influence of two-phase anaerobic digestion on fate of selected antibiotic resistance genes and class I integrons in municipal wastewater sludge[J]. Bioresource Technology, 2016, 211:414-421.
[32]	闫雷, 闫璐, 孙睿, 等. 碱预处理对污泥中抗生素抗性基因丰度的影响[J]. 中国给水排水, 2014,30(19):99-102.
[33]	SONG S Q, JIANG M Y, YAO J, et al. Alkaline-thermal pretreatment of spectinomycin mycelial residues:insights on anaerobic biodegradability and the fate of antibiotic resistance genes[J]. Chemosphere, 2020, 261:127821.
[34]	WANG M L, LI R Y, ZHAO Q. Distribution and removal of antibiotic resistance genes during anaerobic sludge digestion with alkaline, thermal hydrolysis and ultrasonic pretreatments[J]. Frontiers of Environmental Science & Engineering, 2019, 13(3):43-53.
[35]	PEI J, YAO H, WANG H, et al. Comparison of ozone and thermal hydrolysis combined with anaerobic digestion for municipal and pharmaceutical waste sludge with tetracycline resistance genes[J]. Water Research, 2016, 99:122-128.
[36]	MA Y J, WILSON C A, NOVAK J T, et al. Effect of various sludge digestion conditions on sulfonamide, macrolide, and tetracycline resistance genes and class Ⅰ integrons[J]. Environmental Science & Technology, 2011, 45(18):7855-7861.
[37]	CAI C, HUI X S, YANG W, et al. Implications for mitigation of antibiotic resistance:differential response of intracellular and extracellular antibiotic resistance genes to sludge fermentation coupled with thermal hydrolysis[J]. Water Research, 2021, 209:117876.
[38]	SUN C X, LI W, CHEN Z, et al. Responses of antibiotics, antibiotic resistance genes, and mobile genetic elements in sewage sludge to thermal hydrolysis pre-treatment and various anaerobic digestion conditions[J]. Environment International, 2019, 133:105156.
[39]	TONG J, FANG P, ZHANG J Y, et al. Microbial community evolution and fate of antibiotic resistance genes during sludge treatment in two full-scale anaerobic digestion plants with thermal hydrolysis pretreatment[J]. Bioresource Technology, 2019, 288:121575.
[40]	ZHANG J Y, CHEN M X, SUI Q W, et al. Fate of antibiotic resistance genes and its drivers during anaerobic co-digestion of food waste and sewage sludge based on microwave pretreatment[J]. Bioresource Technology, 2016, 217:28-36.
[41]	ZHANG L, LOH K C, ZHANG J X. Jointly reducing antibiotic resistance genes and improving methane yield in anaerobic digestion of chicken manure by feedstock microwave pretreatment and activated carbon supplementation[J]. Chemical Engineering Journal, 2019, 372:815-824.
[42]	ZHANG J Y, LIU J B, WANG Y W, et al. Profiles and drivers of antibiotic resistance genes distribution in one-stage and two-stage sludge anaerobic digestion based on microwave-H₂O₂ pretreatment[J]. Bioresource Technology, 2017, 241:573-581.
[43]	TONG J, LIU J B, ZHENG X, et al. Fate of antibiotic resistance bacteria and genes during enhanced anaerobic digestion of sewage sludge by microwave pretreatment[J]. Bioresource Technology, 2016, 217:37-43.
[44]	HU Y M, SHEN Y P, WANG J L. Pretreatment of antibiotic fermentation residues by combined ultrasound and alkali for enhancing biohydrogen production[J]. Journal of Cleaner Production, 2020, 268:122190.
[45]	HUANG J J, LIANG J L, YANG X, et al. Ultrasonic coupled bioleaching pretreatment for enhancing sewage sludge dewatering:simultaneously mitigating antibiotic resistant genes and changing microbial communities[J]. Ecotoxicology and Environmental Safety, 2020, 193:110349.
[46]	CHEN X J, TANG R, WANG Y L, et al. Effect of ultrasonic and ozone pretreatment on the fate of enteric indicator bacteria and antibiotic resistance genes, and anaerobic digestion of dairy wastewater[J]. Bioresource Technology, 2021, 320:124356.
[47]	ZHAO Q, LI M, ZHANG K F, et al. Effect of ultrasound irradiation combined with ozone pretreatment on the anaerobic digestion for the biosludge exposed to trace-level levofloxacin:degradation, microbial community and ARGs analysis[J]. Journal of Environmental Management, 2020, 262:110356.
[48]	ZHANG Z H, LI X, LIU H, et al. Free ammonia pretreatment enhances the removal of antibiotic resistance genes in anaerobic sludge digestion[J]. Chemosphere, 2021, 279:130910.
[49]	HUANG H N, ZHENG X, CHEN Y G, et al. Alkaline fermentation of waste sludge causes a significant reduction of antibiotic resistance genes in anaerobic reactors[J]. Science of the Total Environment, 2017, 580:380-387.
[50]	李慧莉, 武彩云, 唐安平, 等. 不同污泥在微波预处理-厌氧消化过程中抗性基因分布及菌群结构演替[J]. 环境科学, 2021, 42(1):323-332.
[51]	储思琴, 马佳莹, 徐玉璐, 等. 零价铁在有机固废厌氧消化过程中的应用研究进展[J]. 环境工程, 2021, 39(8):141-149.
[52]	MA J, GU J, WANG X, et al. Effects of nano-zerovalent iron on antibiotic resistance genes during the anaerobic digestion of cattle manure[J]. Bioresource Technology, 2019, 289:121688.
[53]	XIANG Y P, YANG Z H, ZHANG Y R, et al. Influence of nanoscale zero-valent iron and magnetite nanoparticles on anaerobic digestion performance and macrolide, aminoglycoside, beta-lactam resistance genes reduction[J]. Bioresource Technology, 2019, 294:122139.
[54]	魏欣, 薛顺利, 杨帆, 等. 零价铁对污泥高温厌氧消化过程中四环素抗性基因及第一类整合子的削减影响[J]. 环境科学, 2017, 38(2):697-702.
[55]	杨帆, 徐雯丽, 钱雅洁, 等. 零价铁对污泥厌氧消化过程中四环素抗性基因水平转移的作用影响[J]. 环境科学, 2018, 39(4):1748-1755.
[56]	王攀, 杜晓璐, 陈锡腾, 等. Fe⁰对污泥接种餐厨垃圾厌氧发酵及抗生素抗性基因的影响[J]. 环境工程, 2019, 37(7):178-182.
[57]	BANIAMERIAN H, ISFAHANI P G, TSAPEKOS P, et al. Application of nano-structured materials in anaerobic digestion:current status and perspectives[J]. Chemosphere, 2019, 229:188-199.
[58]	WANG T, ZHANG D, DAI L L, et al. Effects of metal nanoparticles on methane production from waste-activated sludge and microorganism community shift in anaerobic granular sludge[J]. Scientific Reports, 2016, 6:25857.
[59]	ZHANG J Y, WANG Z Y, LU T D, et al. Response and mechanisms of the performance and fate of antibiotic resistance genes to nano-magnetite during anaerobic digestion of swine manure[J]. Journal of Hazardous Materials, 2019, 366:192-201.
[60]	ZHANG J Y, LU T D, WANG Z Y, et al. Effects of magnetite on anaerobic digestion of swine manure:attention to methane production and fate of antibiotic resistance genes[J]. Bioresource Technology, 2019, 291:121847.
[61]	ZHANG Y R, XU R, XIANG Y P, et al. Addition of nanoparticles increases the abundance of mobile genetic elements and changes microbial community in the sludge anaerobic digestion system[J]. Journal of Hazardous Materials, 2021, 405:124206.
[62]	QIU Z G, YU Y M, CHEN Z L, et al. Nanoalumina promotes the horizontal transfer of multiresistance genes mediated by plasmids across genera[J]. Proceedings of the National Academy of Sciences, USA, 2012, 109(13):4944-4949.
[63]	LIU X M, TANG J C, SONG B R, et al. Exposure to Al₂O₃ nanoparticles facilitates conjugative transfer of antibiotic resistance genes from Escherichia coli to Streptomyces[J]. Nanotoxicology, 2019, 13(10):1422-1436.
[64]	马佳莹, 王盼亮, 汪冰寒, 等. 活性炭对城市有机固废厌氧消化过程抗生素抗性基因行为特征的影响[J]. 环境科学, 2021, 42(5):2413-2421.
[65]	ZHANG J X, MAO F J, LOH K C, et al. Evaluating the effects of activated carbon on methane generation and the fate of antibiotic resistant genes and class Ⅰ integrons during anaerobic digestion of solid organic wastes[J]. Bioresource Technology, 2018, 249:729-736.
[66]	SUN W, GU J, WANG X J, et al. Impacts of biochar on the environmental risk of antibiotic resistance genes and mobile genetic elements during anaerobic digestion of cattle farm wastewater[J]. Bioresource Technology, 2018, 256:342-349.
[67]	ZHANG J Y, SUI Q W, ZHONG H, et al. Impacts of zero valent iron, natural zeolite and Dnase on the fate of antibiotic resistance genes during thermophilic and mesophilic anaerobic digestion of swine manure[J]. Bioresource Technology, 2018, 258:135-141.
[68]	付嘉琦, 林敏, 闫冰, 等. 污泥厌氧发酵外源添加剂研究进展[J]. 能源研究与管理, 2019(4):17-19.
[69]	郭斯韬, 钱燕云, 徐莉柯, 等. 氮磷对污泥厌氧消化过程中抗生素抗性基因行为特征的影响[C]//2014中国环境科学学会学术年会(第十二章), 2014:7196-7203.
[70]	WANG P, ZHENG Y, LIN P R, et al. Effects of graphite, graphene, and graphene oxide on the anaerobic co-digestion of sewage sludge and food waste:attention to methane production and the fate of antibiotic resistance genes[J]. Bioresource Technology, 2021, 339:125585.
[71]	SHIN J, RHEE C, SHIN J, et al. Determining the composition of bacterial community and relative abundance of specific antibiotics resistance genes via thermophilic anaerobic digestion of sewage sludge[J]. Bioresource Technology, 2020, 311:123510.