Anammox用于主流污水处理的挑战与对策

蒙小俊; 韩勇; 黄志贵; 龚晓松

doi:10.13205/j.hjgc.202210027

Anammox用于主流污水处理的挑战与对策

doi: 10.13205/j.hjgc.202210027

安康学院旅游与资源环境学院, 陕西安康 725000

基金项目:

陕西省科技厅青年项目(2021JQ-835)

安康学院高层次人才科研专项经费(2016AYQDZR09)

陕西省教育厅专项科研计划项目(JK0007)

详细信息

作者简介:
蒙小俊(1981-),男,博士,主要研究方向为环境微生物技术与水污染控制工程。yanjiushengmengjun@163.com

通讯作者:
蒙小俊(1981-),男,博士,主要研究方向为环境微生物技术与水污染控制工程。yanjiushengmengjun@163.com

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出版历程
- 收稿日期: 2021-12-16

CHALLENGES AND SOLUTIONS OF ANAMMOX IN MAINSTREAM WASTEWATER TREATMENT PLANTS

School of Tourism and Environment, Ankang University, Ankang 725000, China

摘要

摘要: 厌氧氨氧化技术(Anammox)在主流污水处理厂(WWTPs)中脱氮具有巨大应用前景,在高效节能、污泥减量和温室气体减排等方面具有显著优势,成为主流污水处理领域的研究焦点和热点。概述了主流污水Anammox,分析了厌氧氨氧化菌(AAOB)存在于主流污水中的可能原因;针对阻碍其实现Anammox所面临的低温、亚硝酸盐和脱氮效率等问题进行深入分析并提出了解决对策,认为实现高纬度地区主流Anammox更适用短程反硝化耦合Anammox工艺(PDA);提出了实现高纬度地区主流Anammox的工艺路线。AAOB生物特性分析、PDA颗粒污泥/生物膜的形成机制和作用、PDA工艺的中试和现场应用以及主流WWTPs PDA的原位实现等是未来主流Anammox研究的重点。
- 污水处理厂 /
- 生物脱氮 /
- 厌氧氨氧化菌 /
- 短程硝化-厌氧氨氧化 /
- 短程反硝化-厌氧氨氧化
Abstract: Anammox technology has great application prospects for nitrogen removal in mainstream wastewater treatment plants (WWTPs). It has significant advantages in terms of high efficiency and energy saving, sludge reduction and greenhouse gas emission reduction, and has become the research focus and hot spot in the mainstream wastewater treatment field. This article summarized the mainstream wastewater Anammox process, analyzed the possible reasons for the presence of anaerobic ammonia-oxidizing bacteria (AAOB) in the general wastewater, and conducted an in-depth analysis on low temperature, nitrite and nitrogen removal efficiency that hindered the realization of Anammox, and proposed countermeasures to realize mainstream Anammox. It considered that the realization of high-latitude mainstream Anammox was more suitable for use of PDA (partial denitrification coupled with Anammox). A process route to achieve mainstream Anammox was proposed. AAOB biological characteristics analysis, the formation mechanism and function of PDA granular sludge/biofilm, pilot test and field application of PDA process, and realization of mainstream WWTPs PDA in situ were considered the focus of future mainstream Anammox.
- wastewater treatment plant /
- biological denitrification /
- Anammox bacteria /
- partial-nitrification Anammox /
- partial denitrification Anammox

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参考文献(95)

[1]	YANG Y C, PAN J, ZHOU Z C, et al. Complex microbial nitrogen-cycling networks in three distinct anammox inoculated wastewater treatment systems[J].Water Research,2020,168:115142.
[2]	CHEN Y Z, ZHAO Z C, LIU H, et al. Achieving stable two-stage mainstream partial-nitrification/anammox (PN/A) operation via intermittent aeration[J]. Chemosphere,2020,245:125650.
[3]	AHMAD H A, NI S Q, AHMAD S, et al.Gel immobilization:a strategy to improve the performance of anaerobic ammonium oxidation (anammox) bacteria for nitrogen-rich wastewater treatment[J]. Bioresource Technology,2020,313:123642.
[4]	ZHANG X J, LI D, LIANG Y H, et al. Application of membrane bioreactor for completely autotrophic nitrogen removal over nitrite (CANON) process[J]. Chemosphere,2013,93(11):2832-2838.
[5]	ZENG M, YANG J F, LIX F, et al. Enhancement of mainstream nitrogen removal via simultaneous partial nitrification, anammox and denitrification by the gel entrapment technique[J]. Environmental Science Water Research & Technology,2020,6(3):767-778.
[6]	PIJUAN M, RIBERA-GUARDIA A, BALCAZAR J L, et al. Effect of COD on mainstream anammox:evaluation of process performance, granule morphology and nitrous oxide production[J]. Science of the Total Environment,2020,712:136372.
[7]	YANG Y C, LI M, HU Z, et al. Deep insights into the green nitrogen removal by anammox in four full-scale WWTPs treating landfill leachate based on 16S rRNA gene and transcripts by 16S rRNA high-throughput sequencing[J].Journal of Cleaner Production,2020,276:124176.
[8]	RIBERA-GUARDIA A, BOSCH L, COROMINAS L, et al. Nitrous oxide and methane emissions from a plug-flow full-scale bioreactor and assessment of its carbon footprint[J]. Journal of Cleaner Production,2019,212:162-172.
[9]	BAHTIAR F, ZHANG X X, WANG Y G, et al. New insight into partial denitrification (PD)-based Anammox process and potential engineering application:a review[J]. Journal of Health and Environmental Research.2020,6(3):79-86.
[10]	KARTAL B, KUENEN J, VAN LOOSDRECHT M.Sewage treatment with anammox[J].Science,2010,328(5979):702-703.
[11]	蒙小俊,郭楠楠,黄志贵. Anammox脱氮工艺应用限制因素的调控策略[J].工业水处理,2021,41(7):13-19.
[12]	YAO Z B, WANG F, WANG C L, et al. Anaerobic ammonium oxidation coupled to ferric iron reduction in the sediment of a eutrophic lake[J]. Environmental Science and Pollution Research,2019,26(15):15084-15094.
[13]	KUYPERS M M M,OLAV SLIEKERS A,LAVIK G,et al.Anaerobic ammonium oxidation by anammox bacteria in the Black Sea[J].Nature,2003,422(6932):608-611.
[14]	LI H, YANG X R, WANG J, et al. Earthwormgut:an overlooked niche for anaerobic ammonium oxidation in agricultural soil[J]. Science of the Total Environment,2021,752:141874.
[15]	DING B J, LUO W Q, QIN Y B, et al. Effects of the addition of nitrogen and phosphorus on anaerobic ammonium oxidation coupled with iron reduction (Feammox) in the farmland soils[J]. Science of the Total Environment, 2020,737:139849.
[16]	WANG X J, YANG R L, ZHANG Z J, et al. Mass balance and bacterial characteristics in an in-situ full-scale swine wastewater treatment system occurring anammox process[J]. Bioresource Technology,2019, 292:122005.
[17]	WANG S Y, ZHU G B, ZHUANG L J, et al. Anaerobic ammonium oxidation is a major N-sink in aquifer systems around the world[J]. The ISME Journal,2020,14(1):151-163.
[18]	ZHANG L,OKABE S. Ecological niche differentiation among anammox bacteria[J].Water Research,2020,171:115468.
[19]	LU H F, LI Y Y, SHAN X Y, et al. A holistic analysis of ANAMMOX process in response to salinity:from adaptation to collapse[J].Separation and Purification Technology,2019,215:342-350.
[20]	ZEKKER I, RAUDKIVI M, ARTEMCHUK O, et al. Mainstream-sidestream wastewater switching promotes anammox nitrogen removal rate in organic-rich, low-temperature streams[J]. Environmental Technology, 2021,42(19):3073-3082.
[21]	WU P, ZHANG X X, WANG X Z, et al. Characterization of the start-up of single and two-stage Anammox processes with real low-strength wastewater treatment[J]. Chemosphere, 2020,245:125572.
[22]	JI J T, PENG Y Z, WANG B, et al. Synergistic partial-denitrification, Anammox, and in-situ fermentation (SPDAF) process for advanced nitrogen removal from domestic and nitrate-containing wastewater[J]. Environmental Science & Technology,2020,54(6):3702-3713.
[23]	WANG S Y, PENG Y Z, MA B, et al. Anaerobic ammonium oxidation in traditional municipal wastewater treatment plants with low-strength ammonium loading:widespread but overlooked[J]. Water Research, 2015, 84:66-75.
[24]	NEJIDAT A, DIZA-RECK D, MASSALHA N, et al. Abundance and diversity of anammox bacteria in a mainstream municipal wastewater treatment plant[J]. Applied Microbiology and Biotechnology,2018,102(15):6713-6723.
[25]	蒙小俊,龚晓松,王秋利.主流城镇污水处理厂厌氧氨氧化菌多样性分析[J].工业水处理,2021,41(12):83-88.
[26]	WINKLER M K H, YANG J J, KLEEREBEZEM R, et al. Nitrate reduction by organotrophic Anammox bacteria in a nitritation/anammox granular sludge and a moving bed biofilm reactor[J].Bioresource Technology,2012,114:217-223.
[27]	LAURENI M, FALAS P, ROBIN O, et al.Mainstream partial nitritation and anammox:long-term process stability and effluent quality at low temperatures[J]. Water Research,2016,101:628-639.
[28]	ZHANG T Q, WEI N, WU G X. Autotrophic nitrogen removal and potential microbial interactions in anammox systems with different ammonia and organic carbon concentrations[J].Journal of Water Process Engineering,2020,37:101493.
[29]	KARTAL B, VAN NIFERIK L, KELTJENS J T, et al.Anammox-growth physiology, cell biology, and metabolism[J]. Advances in Microbial Physiology, 2012,60:211-262.
[30]	BLACKBURNE R, VADIVELU V M, YUAN Z G, et al. Kinetic characterisation of an enriched Nitrospira culture with comparison to Nitrobacter[J]. Water Research,2007,41(14):3033-3042.
[31]	LI Y Y, YU T, KANG D, et al. Sources of anammox granular sludge and their sustainability in treating low-strength wastewater[J]. Chemosphere, 2019,226:229-237.
[32]	YAO R D, YUAN Q, WANG K J.Effective inhibition prevention strategy for the enrichment of anammox bacteria with low concentrations of substrates at 25℃[J]. Journal of Water Process Engineering, 2020,37:101514.
[33]	LI X L, ZHANG J, ZHANG X Y, et al.Start-up and nitrogen removal performance of CANON and SNAD processes in a pilot-scale oxidation ditch reactor[J]. Process Biochemistry,2019,84:134-142.
[34]	JIA M S, SOLON K, VANDEPLASSCHE D, et al. Model-based evaluation of an integrated high-rate activated sludge and mainstream anammox system[J]. Chemical Engineering Journal,2020,382:122878.
[35]	PIJUAN M, RIBERA-GUARDIA A, BALCAZAR J L, et al. Effect of COD on mainstream anammox:evaluation of process performance, granule morphology and nitrous oxide production[J]. Science of the Total Environment,2020,712:136372.
[36]	WANG Z B, LIU X L, BU C N, et al. Microbial diversity reveals the partial denitrification-anammox process serves as a new pathway in the first mainstream anammox plant[J]. Science of the Total Environment,2020,764:142917.
[37]	LAUREN M, WEISSBRODT D G, SZIVAK I, et al.Activity and growth of anammox biomass on aerobically pre-treated municipal wastewater[J]. Water Research,2015,80:325-336.
[38]	KWAK W, ROUT P R, LEE E, et al. Influence of hydraulic retention time and temperature on the performance of an anaerobic ammonium oxidation fluidized bed membrane bioreactor for low-strength ammonia wastewater treatment[J]. Chemical Engineering Journal, 2020,386:123992.
[39]	GANI K M, AWOLUSI O O, KHAN A A, et al. Potential strategies for the mainstream application of anammox in treatment of anaerobic effluents:a review[J]. Critical Reviews in Environmental Science and Technology,2021,51(21):2567-2594.
[40]	GILBERT E M, AGRAWAL S, KARST S M, et al. Low-temperature partial nitritation/anammox in a moving bed biofilm reactor treating low strength wastewater[J]. Environmental Science & Technology, 2014,48(15):8784-8792.
[41]	LI J W, PENG Y Z, ZHANG L, et al. Quantify the contribution of anammox for enhanced nitrogen removal through metagenomic analysis and mass balance in an anoxic moving bed biofilm reactor[J]. Water Research,2019,160:178-187.
[42]	PERSSON F, WILN B M, SUAREZ C, et al.Community structure of partial nitritation-anammox biofilms at decreasing substrate concentrations and low temperature[J]. Microbial Biotechnology, 2017,10(4):761-772.
[43]	CHEN H, LIU T, LI J, et al. Larger anammox granules not only harbor higher species diversity but also support more functional diversity[J]. Environmental Science & Technology,2020,54(22):14664-14673.
[44]	LIU W R, SONG J J, WANG J F, et al. Comparing nitrite-limited and ammonium-limited anammox processes treating low-strength wastewater:functional and population heterogeneity[J]. Chemosphere,2020,258:127290.
[45]	LI B L, WANG Y, LI J T, et al. The symbiosis of anaerobic ammonium oxidation bacteria and heterotrophic denitrification bacteria in a size-fractioned single-stage partial nitrification/anammox reactor[J]. Biochemical Engineering Journal, 2019,151:107353.
[46]	MIAO Y Y, ZHANG J H, PENG Y Z, et al. An improved start-up strategy for mainstream anammox process through inoculating ordinary nitrification sludge and a small amount of anammox sludge[J]. Journal of Hazardous Materials, 2020,384:121325.
[47]	LIU W R, YANG D H, CHEN W J, et al. High-throughput sequencing-based microbial characterization of size fractionated biomass in an anoxic anammox reactor for low-strength wastewater at low temperatures[J].Bioresource Technology,2017,231(1):45-52.
[48]	XUE Y, MA H Y, KONG Z, et al. Bulking and floatation of the anammox-HAP granule caused by low phosphate concentration in the anammox reactor of expanded granular sludge bed(EGSB)[J]. Bioresource Technology,2020,310:123421.
[49]	WANG W G, WANG H, JIANG Z W, et al. Visual evidence for anammox granules expanding their size by aggregation of anammox micro-granules[J]. Science of the Total Environment,2020,745:141052.
[50]	CHENG Y F, ZHANG Z Z, LI G F, et al. Anammox granules acclimatized to mainstream conditions can achieve a volumetric nitrogen removal rate comparable to sidestream systems[J]. Environmental Science & Technology,2020, 54(20):12959-12966.
[51]	REINO C, SUAREZ-OJEDA M E, PÉREZ J, et al. Stable long-term operation of an upflow anammox sludge bed reactor at mainstream conditions[J]. Water Research, 2018, 128:331-340.
[52]	WANG S K, ISHII K, YU H, et al. Stable nitrogen removal by anammox process after rapid temperature drops:insights from metagenomics and metaproteomics[J]. Bioresource Technology,2021,320:124231.
[53]	LI M C, SONG Y, SHEN W, et al. The performance of an anaerobic ammonium oxidation upflow anaerobic sludge blanket reactor during natural periodic temperature variations[J]. Bioresource Technology,2019,293:122039.
[54]	JUAN-DIZA X, PEREZ J, CARRERAB J. Effective dampening of temperature effects in an anammox reactor treating real mainstream wastewater[J].Journal of Water Process Engineering, 2020,40:101853.
[55]	JETTEN M S M, HORN S J, LOOSDRECHT M C M V.Towards a more sustainable municipal wastewater treatment system[J]. Water Science and Technology,1997,35(9):171-180.
[56]	DU R, CAO S B, ZHANG H Y, et al.Flexible nitrite supply alternative for mainstream anammox:advances in enhancing process stability[J]. Environmental Science & Technology,2020,54 (10):6353-6364.
[57]	LACKNER S,GILBERT E M,VLAEMINCK S E,et al. Full-scale partial nitritation/Anammox experiences an application survey[J].Water Research,2014,55(10):292-303.
[58]	MIAO Y Y, ZHANG L, LI B K, et al. Enhancing ammonium oxidizing bacteria activity was key to single-stage partial nitrification anammox system treating low-strength sewage under intermittent aeration condition[J]. Bioresource Technology,2017, 231:36-44.
[59]	MIAO Y Y, PENG Y Z, ZHANG L, et al. Partial nitrification-anammox (PNA) treating sewage with intermittent aeration mode:effect of influent C/N ratios[J]. Chemical Engineering Journal,2018,334:664-672.
[60]	CAO Y S, VAN LOOSDRECHT M C M, DAIGGER G T. Mainstream partial nitritation anammox in municipal wastewater treatment:status, bottlenecks, and further studies[J]. Applied Microbiology and Biotechnology,2017,101 (4):1365-1383.
[61]	PREZ J, ISANTA E, CARRERA J.Would a two-stage N-removal be a suitable technology to implement at full scale the use of anammox for sewage treatment?[J]. Water Science and Technology, 2015,72(6):858-864.
[62]	LIU W R, YANG D H, SHEN Y L, et al. Two-stage partial nitritation-anammox process for high-rate mainstream deammonification[J]. Applied Microbiology and Biotechnology, 2018,102 (18):8079-8091.
[63]	ZHU G B, WANG S Y, FENG X J, et al. Anammox bacterial abundance, biodiversity and activity in a constructed wetland[J]. Environmental Science & Technology,2011,45 (23):9951-9958.
[64]	XIANG T, GAO D W, WANG X L. Performance and microbial community analysis of two sludge type reactors in achieving mainstream deammonification with hydrazine addition[J]. Science of the Total Environment, 2020,715:136377.
[65]	MA Y, PENG Y Z, WANG S Y, et al.Achieving nitrogen removal via nitrite in a pilot-scale continuous pre denitrification plant[J].Water Research, 2009, 43(3):563-572.
[66]	BAO P, WANG S Y, MA B, et al.Achieving partial nitrification by inhibiting the activity of Nitrospira-like bacteria under high DO conditions in an intermittent aeration reactor[J]. Journal of Environmental Sciences, 2017,56(6):71-78.
[67]	ZHOU X Y, LIU X H, HUANG S T, et al.Total inorganic nitrogen removal during the partial/complete nitrification for treating domestic wastewater:removal pathways and main influencing factors[J].Bioresource Technology,2018,256:285-294.
[68]	CHANDRAN K, SMETS B F. Single-step nitrification models erroneously describe batch ammonia oxidation profiles when nitrite oxidation becomes rate limiting[J]. Biotechnology and Bioengineering,2000,68(4):396-406.
[69]	MALOVANYY A, TRELA J, PLAZA E. Mainstream wastewater treatment in integrated fixed film activated sludge (IFAS) reactor by partial nitritation/anammox process[J]. Bioresource Technology, 2015,198(1):478-487.
[70]	LAURENI M, FALAS P, ROBIN O, et al. Mainstream partial nitritation and anammox:long-term process stability and effluent quality at low temperatures[J]. Water Research,2016,101(1):628-639.
[71]	DENG S Y, PENG Y Z, ZHANG L, et al.Advanced nitrogen removal from municipal wastewater via two-stage partial nitrification-simultaneous anammox and denitrification (PN-SAD) process[J]. Bioresource Technology,2020,304:122955.
[72]	ZHANG M, WANG S Y, JI B, et al.Towards mainstream deammonification of municipal wastewater:partial nitrification anammox versus partial denitrification-anammox[J]. Science of the Total Environment,2019,692:393-401.
[73]	WANG X X, ZHAO J, YU D S, et al. Stable nitrite accumulation and phosphorous removal from nitrate and municipal wastewaters in a combined process of endogenous partial denitrification and denitrifying phosphorus removal (EPDPR)[J]. Chemical Engineering Journal,2019,355:560-571.
[74]	MA B, XU X X, GE S J, et al. Reducing carbon source consumption through a novel denitratation/anammox biofilter to remove nitrate from synthetic secondary effluent[J]. Bioresource Technology, 2020, 309:123377.
[75]	DU R, CAO S B, LI B K, et al. Performance and microbial community analysis of a novel DEAMOX based on partial denitrification and anammox treating ammonia and nitrate wastewaters[J]. Water Research,2017,108:46-56.
[76]	CAO S B, DU R, LI B K, et al.Nitrite production from partial-denitrification process fed with low carbon/nitrogen (C/N) domestic wastewater:performance, kinetics and microbial community[J]. Chemical Engineering Journal,2017,326:1186-1196.
[77]	CAO S B, DU R, PENG Y Z, et al. Novel two stage partial denitrification (PD)-Anammox process for tertiary nitrogen removal from low carbon/nitrogen (C/N) municipal sewage[J].Chemical Engineering Journal,2019,362:107-115.
[78]	LIU T, HU S H, GUO J H. Enhancing mainstream nitrogen removal by employing nitrate/nitrite-dependent anaerobic methane oxidation processes[J]. Critical Reviews in Biotechnology,2019,39(5):732-745.
[79]	CAO Y S, van LOOSDRECHT M C M, DAIGGER G T. Mainstream partial nitritation-anammox in municipal wastewater treatment:status, bottlenecks, and further studies[J]. Applied Microbiology and Biotechnology,2017,101(4):1365-1383.
[80]	王建辉,游庆国,申渝,等.短程反硝化-厌氧氨氧化耦合脱氮工艺影响因素与调控研究进展[J].环境化学,2021,40(4):1216-1231.
[81]	JI J T, PENG Y Z, LI X Y, et al. Stable long-term operation and high nitrite accumulation of an endogenous partial denitrification (EPD) granular sludge system under mainstream conditions at low temperature[J]. Bioresource Technology, 2019,289:121634.
[82]	CHEN C J, SUN F Q, ZHANG H Q, et al. Evaluation of COD effect on anammox process and microbial communities in the anaerobic baffled reactor (ABR)[J]. Bioresource Technology,2016,216(1):571-578.
[83]	GU J, YANG Q, LIU Y. Mainstream anammox in a novel A-2B process for energy-efficient municipal wastewater treatment with minimized sludge production[J]. Water Research,2018,138:1-6.
[84]	LIU T, LI J, LIM Z K H, et al. Simultaneous removal of dissolved methane and nitrogen from synthetic mainstream anaerobic effluent[J].Environmental Science & Technology,2020, 54(12):7629-7638.
[85]	XIE G J, LIU T, CAI C, et al. Achieving high-level nitrogen removal in mainstream by coupling anammox with denitrifying anaerobic methane oxidation in a membrane biofilm reactor[J]. Water Research, 2018,131:196-204.
[86]	PENG L, NIE W B, DING J, et al. Denitrifying anaerobic methane oxidation and Anammox process in a membrane aerated membrane bioreactor:kinetic evaluation and optimization[J]. Environmental Science & Technology,2020,54(11):6968-6977.
[87]	STROUS M, HEIJNEN J J, KUENEN J G, et al.The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobic ammonium-oxidizing microorganisms[J].Applied Microbiology and Biotechnology, 1998,50(5):589-596.
[88]	JUAN-DIAZ X, PEREZ J, CARRERA J. Effective dampening of temperature effects in an anammox reactor treating real mainstream wastewater[J]. Journal of Water Process Engineering,2020,40,101853.
[89]	WANG B, PENG Y Z, GUO Y Y, et al. Nitrogen removal from wastewater and external waste activated sludge reutilization/reduction by simultaneoussludge fermentation, denitrification, and anammox (SFDA)[J]. Bioresource Technology,2016,214:284-291.
[90]	RAUDKIVI M, ZEKKER I, RIKMANN E, et al.Nitrite inhibition and limitation-the effect of nitrite spiking on anammox biofilm, suspended and granular biomass[J].Water Science Technology,2017,75(2):313-321.
[91]	陈思宇,张绍青,陈鹏,等.基于短程反硝化的生物脱氮技术研究进展[J].环境工程,2021,35(9):38-44.
[92]	杜睿,彭永臻.城市污水生物脱氮技术变革:厌氧氨氧化的研究与实践新进展[J].中国科学(技术科学),2022(3):389-402.
[93]	CAO S B, OEHMEN A, ZHOU Y. Denitrifiers in mainstream anammox processes:competitors or supporters?[J]. Environmental Science & Technology,2019,53:11063-11065.
[94]	彭永臻,范泽里,杜睿,等.为耦合厌氧氨氧化产生NO^-₂-N的城市污水中试研究[J].北京工业大学报,2020,46(4):329-337.
[95]	谢军祥,姜滢,常尧枫,等.城镇生活污水厌氧氨氧化处理的研究进展[J].化工进展,2020,39(10):4175-4184.