蚯蚓生物滤池削减剩余污泥中ARGs和ARB的效果研究

赵然; 邢美燕; 杨格格; 邢丽波

doi:10.13205/j.hjgc.202212015

蚯蚓生物滤池削减剩余污泥中ARGs和ARB的效果研究

doi: 10.13205/j.hjgc.202212015

1. 同济大学环境科学与工程学院, 上海 200092;
2. 同济大学建筑设计研究院(集团)有限公司, 上海 200092

基金项目:

国家自然科学基金(51679168)

同济大学"双带头人"学术能力提升计划(0400219423)

详细信息

作者简介:
赵然(1999-),女,硕士研究生,主要研究方向为污水生物处理理论与资源化。17317813942@163.com

通讯作者:
邢美燕(1977-),女,博士,副教授,主要研究方向为污水/污泥生物生态处理技术。xingmeiyan@tongji.edu.cn

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- 文章访问数: 145
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- 被引次数: 0
出版历程
- 收稿日期: 2022-02-04
- 网络出版日期: 2023-03-23

REMOVAL EFFECT OF VERMIFILTRATION ON ARGs AND ARB IN EXCESS SLUDGE

1. College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China;
2. Tongji Architectural Design (Group) Co., Ltd, Shanghai 200092, China

摘要

摘要: 蚯蚓生物滤池(vermifiltration, VF)是一种低碳、环境友好的生态技术。基于其良好的剩余污泥减量化效果,以不加蚯蚓的普通生物滤池(biofilter, BF)为对照,探究蚯蚓生物滤池对新型污染物——抗生素抗性菌(antibiotic resistance bacteria, ARB)和抗生素抗性基因(antibiotic resistance genes, ARGs)的去除效果。结果表明:与BF相比,VF对ARB(四环素类抗性菌、磺胺类抗性菌和双抗性抗性菌)的去除率增加25百分点以上,对ARGs(tetO、tetM、tetQ、tetW、sul1、sul2)去除率增加20百分点以上,且具有选择性。VF对intI1去除率高达98.24%,大大降低了ARGs的水平转移和传播风险。夏、冬季,VF对ARGs和ARB去除率比BF高15百分点以上,说明蚯蚓的加入减弱了低、高温对生物滤池的抑制作用。蚯蚓生物滤池能有效提高污泥中ARGs和ARB去除率,为剩余污泥的无害化处理提供参考。
- 蚯蚓生物滤池 /
- ARGs /
- ARB /
- 定量PCR
Abstract: Vermifiltration (VF) is a low-carbon and eco-friendly technology. Based on the excellent excess sludge reduction efficacy, the removal effects of newly emerging pollutants, antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARB) were evaluated, with a conventional biofilter (BF, no earthworm) as a control. The results indicated that the VF increased the removal rate of ARB (tetracycline resistant bacteria, sulfamethoxazole resistance bacteria and double resistance bacteria) by more than 25%, and exhibited higher but selective ARGs (tetO, tetM, tetQ, tetW, sul1, sul2) removal rates, generally increased by more than 20%, compared to the BF. Moreover, the average removal rate of intI1 by VF was 98.24%, reducing the risk of horizontal transmission of ARGs. In summer and winter, compared with the BF, the removal of ARGs and ARB by the VF increased by more than 15%, indicating that the addition of earthworms weakened the inhibitory effect of low and high temperatures. Conclusively, vermifiltration could effectively improve the removal rates of various ARGs and ARB in the excess sludge by the excellent excess sludge removal, which provides a reference for the ARGs and ARB removal assisted by the earthworms.
- vermifiltration /
- ARGs /
- ARB /
- quantitative PCR

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参考文献(37)

[1]	苏志国,张衍,代天娇,等.环境中抗生素抗性基因与Ⅰ型整合子的研究进展[J].微生物学通报,2018,45(10):2217-2233.
[2]	付星宇,汪庆,毕聪聪,等.大气环境中抗生素耐药菌的来源与传播扩散研究进展[J].中国抗生素杂志,2021,46(9):821-828.
[3]	姚鹏城,陈嘉瑜,张永明,等.抗生素抗性基因在生活及工业混合废水处理系统中的分布和去除[J].生态毒理学报,2020,15(1):201-208.
[4]	李十盛,高会,赵富强,等.水产养殖环境中抗生素抗性基因的研究进展[J].中国环境科学,2021,41(11):5314-5325.
[5]	黄福义,周曙仡聃,王佳妮,等.不同作物农田土壤抗生素抗性基因多样性[J].环境科学,2021,42(6):2975-2980.
[6]	李佳楠.城市污水系统中典型抗生素、抗性基因和抗性细菌的丰度与关联性研究[D].杭州:浙江大学,2014.
[7]	包樱钰,谢辉,陈吕军,等.城镇污水处理系统中4种抗生素抗性基因的沿程变化[J].北京大学学报(自然科学版),2020,56(4 ):772-776.
[8]	CHEN H, ZHANG M M. Occurrence and removal of antibiotic resistance genes in municipal wastewater and rural domestic sewage treatment systems in eastern China[J]. Environment International,2013,55:9-14.
[9]	PRUDEN A. Balancing water sustainability and public health goals in the face of growing concerns about antibiotic resistance[J]. Environmental Science & Technology,2014,48(1):5-14.
[10]	樊慧杰.蚯蚓体腔液及脱氧核糖核酸酶的抗菌机制研究[D].太原:山西医科大学,2008.
[11]	CHAO H Z, ZHENG X X, XIA R, et al. Incubation trial indicated the earthworm intestinal bacteria as promising biodigestor for mitigating tetracycline resistance risk in anthropogenic disturbed forest soil[J]. Science of the Total Environment,2021,798:149337.
[12]	杨思德.蚯蚓-微生物对金霉素污染土壤的协同修复作用与机制研究[D].长春:吉林农业大学,2021.
[13]	张嘉炫.蚯蚓堆肥对含铜猪粪中耐药基因和微生物群落的影响[D].广州:华南农业大学,2018.
[14]	彭兰生,关孟欣,黄魁,等.蚯蚓摄食污泥对其肠道功能区微生物种群及耐药基因的影响[J].中国环境科学,2022,42(1):465-473.
[15]	潘赛,邢美燕,王寅,等.蚯蚓生物滤池污水处理研究进展[J].中国给水排水,2015,31(22):22-26.
[16]	赵丽敏.蚯蚓生物滤池处理剩余污泥工艺性能及降解机制研究[D].上海:同济大学,2009.
[17]	苏超.不同消毒方法削减城市污水处理厂尾水中抗生素抗性基因的比较研究[D].杭州:浙江大学,2015.
[18]	张明美.污水处理系统中抗生素抗性基因污染研究[D].杭州:浙江大学,2013.
[19]	LUO Y, MAO D Q, RYSZ M, et al. Trends in antibiotic resistance genes occurrence in the Haihe River, China[J]. Environmental Science & Technology,2010,44(19):7220-7225.
[20]	AMINOV R I, GARRIGUES-JEANJEAN N, MACKIE R I. Molecular ecology of tetracycline resistance:development and validation of primers for detection of tetracycline resistance genes encoding ribosomal protection proteins[J]. Applied and Environmental Microbiology,2001,67(1):22-32.
[21]	GHOSH S, RAMSDEN S J, LAPARA T M. The role of anaerobic digestion in controlling the release of tetracycline resistance genes and class 1 integrons from municipal wastewater treatment plants[J]. Applied Microbiology and Biotechnology,2009,84(4):791-796.
[22]	郑雅丹,陆雨凡,吴安斐,等.蚯蚓生物滤池处理剩余污泥的脱水性能研究[J].中国给水排水,2016,32(3):1-5 ,10.
[23]	梁建军,彭俊,侯淑媛.蚯蚓生态滤池处理农村生活污水试验研究[J].中国给水排水,2016,32(11):16-19.
[24]	罗义,周启星.抗生素抗性基因(ARGs):一种新型环境污染物[J].环境科学学报,2008,28(8):1499-1505.
[25]	段然.哈尔滨市污水厂中抗生素抗性基因分布及强化去除研究[D].上海:上海师范大学,2014.
[26]	ZHAO C H, XING M Y, YANG J, et al. Microbial community structure and metabolic property of biofilms in vermifiltration for liquid-state sludge stabilization using PLFA profiles[J]. Bioresource Technology,2014,151:340-346.
[27]	刘静.蚯蚓生物滤池处理剩余污泥系统微生物群落特征及营养级关系[D].上海:同济大学,2013.
[28]	CUI G Y, BHAT S A, LI W J, et al. Gut digestion of earthworms significantly attenuates cell-free and-associated antibiotic resistance genes in excess activated sludge by affecting bacterial profiles[J]. Science of the Total Environment,2019,691(C):644-653.
[29]	LUNA V A, ROBERTS M C. The presence of the tetO gene in a variety of tetracycline-resistant Streptococcus pneumoniae serotypes from Washington State[J]. The Journal of Antimicrobial Chemotherapy,1998,42(5):613-619.
[30]	LIAO H P, LU X M, RENSING C, et al. Hyperthermophilic composting accelerates the removal of antibiotic resistance genes and mobile genetic elements in sewage sludge[J]. Environmental Science & Technology,2018,52(1):266-276.
[31]	CHEN J, MICHEL F C, SREEVATSAN S, et al. Occurrence and persistence of erythromycin resistance genes (erm) and tetracycline resistance genes (tet) in waste treatment systems on swine farms[J]. Microbial Ecology,2010,60(3):479-486.
[32]	张燕茹.厌氧消化强化技术对剩余污泥中微生物和抗生素抗性基因的影响研究[D].长沙:湖南大学,2020.
[33]	NARCISO-DA-ROCHA C, MANAIA C M, MANAIA. Multidrug resistance phenotypes are widespread over different bacterial taxonomic groups thriving in surface water[J]. Science of the Total Environment,2016,563/564:1-9.
[34]	WANG Y, WANG X J, LI Y, et al. Effects of coexistence of tetracycline, copper and microplastics on the fate of antibiotic resistance genes in manured soil[J]. Science of the Total Environment,2021,790:148087.
[35]	左怡如.解偶联剂对污泥减量化过程中抗生素抗性基因的影响研究[D].杭州:浙江大学,2016.
[36]	HUANG K, XIA H, WU Y, et al. Effects of earthworms on the fate of tetracycline and fluoroquinolone resistance genes of sewage sludge during vermicomposting[J]. Bioresource Technology,2018,259:32-39.
[37]	胡静.城市污水处理厂中抗生素抗性细菌和抗生素抗性基因的污染特征研究[D].青岛:青岛理工大学,2021.