具有铁氧化和好氧反硝化功能的<i>Achromobacter denitrificans</i> strain 2-5对序批式反应器生物脱氮性能及群落结构的影响

易宏学; 李杰; 王亚娥; 赵炜; 谢慧娜; 张文莉; 权海荣; 慕浩; 胡凯耀

doi:10.13205/j.hjgc.202212028

具有铁氧化和好氧反硝化功能的Achromobacter denitrificans strain 2-5对序批式反应器生物脱氮性能及群落结构的影响

doi: 10.13205/j.hjgc.202212028

1. 兰州交通大学环境与市政工程学院, 兰州 730070;
2. 西北综合勘察设计研究院, 西安 710000;
3. 中铁第一勘察设计院集团有限公司, 西安 710000

基金项目:

国家自然科学基金"生物海绵铁体系中铁与微生物协同同步反硝化作用机制及其应用基础研究"(51768032)

详细信息

作者简介:
易宏学(1997-),男,硕士研究生,主要研究方向为水污染控制。597107333@qq.com

通讯作者:
李杰(1964-),男,教授,主要研究方向为水污染控制。wye@mail.lzjtu.cn

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出版历程
- 收稿日期: 2021-12-31
- 网络出版日期: 2023-03-23

EFFECTS OF ACHROMOBACTER DENITRIFICANS STRAIN 2-5 WITH IRON OXIDATION AND AEROBIC DENITRIFICATION FUNCTION ON BIOLOGICAL NITROGEN REMOVAL PERFORMANCE AND COMMUNITY STRUCTURE IN A SEQUENCING BATCH REACTOR

1. School of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;
2. Northwest Research Institute of Engineering Investiga, Xi'an 710000, China;
3. China Railway First Survey and Design Institute Group Co., Ltd, Xi'an 710000, China

摘要

摘要: 对1株具有铁氧化和好氧反硝化功能的反硝化无色杆菌2-5(Achromobacter denitrificans strain 2-5)强化SBR反应器脱氮性能与细菌群落结构的影响进行研究。结果表明:在SBR反应器中,添加Fe⁰和Achromobacter denitrificans strain 2-5能提高SBR反应器对NH₄⁺-N和TN的去除效果,与普通SBR反应器相比,NH₄⁺-N和TN的平均去除率分别提高了4.13%、15.73%。通过高通量测序分析,发现各反应器微生物群落结构组成存在差异。从门水平来看,变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)、放线菌门(Actinobacteria)、厚壁菌门(Firmicutes)为优势菌门。添加Fe⁰和Achromobacter denitrificans strain 2-5使反应器中具有好氧反硝化功能的细菌多样性增加,强化了SBR反应器的脱氮效果,研究结果为Fe⁰促进好氧反硝化菌生长,强化污水脱氮提供了依据,有利于好氧反硝化菌的实际应用。
- 好氧反硝化菌 /
- 零价铁 /
- 反硝化无色杆菌2-5 /
- 生物强化 /
- 群落结构
Abstract: The effect of an Achromobacter denitrificans strain 2-5, with the function of iron oxidation and aerobic denitrification, on enhancing the denitrification performance of an SBR reactor and its bacterial community structure was studied in this paper. The results showed that the addition of Fe⁰ and Achromobacter denitricans strain 2-5 could improve the removal efficiency of NH₄⁺-N and TN in the SBR reactor. Compared with the ordinary SBR reactors, the average removal rates of NH₄⁺-N and TN were increased by 4.13% and 15.73%. Through high-throughput sequencing analysis, differences were found in the composition of microbial community structure in each reactor. At the phylum level, Proteobacteria, Bacteroidetes, Actinobacteria and Firmicutes were the dominant phyla. The addition of Fe⁰ and Achromobacter denitrificans strain 2-5 increased the diversity of bacteria with aerobic denitrification function in the reactor and strengthened the denitrification effect of the SBR reactor. The research results provide a theoretical basis for Fe⁰ to promote the metabolic growth of aerobic denitrifying bacteria and are conducive to the practical application of aerobic denitrifying bacteria.
- aerobic denitrification /
- zero-valent iron /
- Achromobacter denitrificans strain 2-5 /
- bioaugmentation /
- community structure

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