不同深度复合垂直流人工湿地脱氮性能比较

黄秋瑾; 肖莹; 王亚芬; 吴振斌; 周巧红

doi:10.13205/j.hjgc.202301020

不同深度复合垂直流人工湿地脱氮性能比较

doi: 10.13205/j.hjgc.202301020

1. 中国地质大学(武汉) 环境学院, 武汉 430074;
2. 中国科学院水生生物研究所淡水生态与生物技术国家重点实验室, 武汉 430072

基金项目:

国家自然科学基金项目（51509225）

详细信息

作者简介:
黄秋瑾(1998-),女,硕士研究生,主要研究方向为水环境生态修复。huangqiujin@cug.edu.cn

通讯作者:
王亚芬(1982-),女,副教授,主要研究方向为水环境污染与生态修复。wangyf@cug.edu.cn

计量
- 文章访问数: 86
- HTML全文浏览量: 6
- PDF下载量: 7
- 被引次数: 0
出版历程
- 收稿日期: 2022-04-15
- 网络出版日期: 2023-03-23

NITROGEN REMOVAL PERFORMANCE OF COMPOSITE VERTICAL FLOW CONSTRUCTED WETLAND AT DIFFERENT DEPTHS

1. School of Environmental Studies, China University of Geosciences (Wuhan), Wuhan 430074, China;
2. State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan 430072, China

摘要

摘要: 设计构建了4套分段式复合垂直流人工湿地（IVCW）柱状模拟系统（总程分别为145，185 cm），针对低C/N型人工污水，研究基质层高、添加活性生物质及进水中氮素组成对该系统脱氮效果的影响。结果表明：当水力停留时间为1 d时，4套系统对总氮（TN）去除率均在30%以上，半程基质层设置为95 cm，不添加活性生物质的HI组对TN去除率最高，达到42%；对NH₃-N、NO₃^--N的平均去除率分别为20%~48%、21%~66%，而NO₂^--N在各系统中均发生了不同程度的积累。随着进水中NO₃^--N占比负荷的增加（从1/7到6/7），其NO₃^--N去除率整体呈下降趋势，仅HI组去除率变化较其他组更稳定。但IVCW系统加深后，添加活性生物质反而会降低其TN去除效果。IVCW系统第1段，即下行流上层单元对脱氮贡献最大，高达80%以上；不同处理间强化脱氮效果差异虽不显著，但系统后段其脱氮途径间存在一定差异。相应地，微生物脂肪酸的丰度和种类在第1段最高，其脂肪酸组成与其他段差异最明显；以cy19：0为标志的厌氧细菌在上行流第3~4段逐渐占据优势，且加深组均高于其对照组；单不饱和脂肪酸（MUFA）表征的好氧细菌对IVCW系统脱氮效率贡献更大。
- IVCW /
- 氨态氮 /
- 硝态氮 /
- 微生物群落 /
- 脂肪酸甲酯分析
Abstract: In this project, four sectional IVCW column pilot systems with the column depth of 145 cm and 185cm were built for wastewater with a low C/N ratio, to examine the effects of substrate height, the addition of active biomass and nitrogen composition in influent on nitrogen removal efficiency of the system. Results showed when the hydraulic retention time was 1 d, the total nitrogen (TN) removal rate of the four systems was all above 30%, and when the half-way substrate layer was set at 95 cm, the HI group without active biomass added had the highest TN removal rate of 42%; the average removal rates of NH₃-N and NO₃-N ranged from 20% to 48% and 21% to 66% respectively, while NO₂-N was found accumulated in different degrees in each system. With the increase of the proportion of NO3-N in the influent (from 1/7 to 6/7), the removal rate of NO₃-N in the influent decreased as a whole, and the removal rate of HI group was more stable than that of the other groups. However, after the depth of the IVCW system was increased, adding active biomass will reduce its TN removal effect. The first stage of the IVCW system, i.e. the upper downflow unit, contributed the most to nitrogen removal, up to 80%; although the effect of enhanced nitrogen removal was not significant among different treatments, there were certain differences in the nitrogen removal pathway in the later stages of the system. Accordingly, the abundance and type of microbial fatty acids were the highest in the first segment, and their fatty acid composition was the most obvious difference from other segments; the anaerobic bacteria marked by cy19:0 gradually occupied the dominant position in the 3 to 4 segments of the upstream flow, and the deepened group was higher than the control; the aerobic bacteria characterized by monounsaturated fatty acids (MUFA) contributed more to the nitrogen removal efficiency of the IVCW system.
- IVCW /
- ammonium nitrogen /
- nitrate nitrogen /
- microbial community /
- FAMEs analysis

HTML全文

参考文献(28)

[1]	中华人民共和国生态环境部, 2020年中国生态环境状况年报[Z], 2021-05-24.
[2]	中华人民共和国生态环境部, 2020年中国生态环境统计年报[Z], 2022-02-18.
[3]	张岩, 赵剑文, 宋超, 等. 2019. 低碳氮比污水脱氮技术最新进展[J]. 中国资源综合利用, 37(3):67-70.
[4]	SUN S P, NACHER C P I, MERKEY B, et al. Effective biological nitrogen removal treatment processes for domestic wastewaters with Low C/N ratios:a review[J]. Environmental Engineering Science, 2010, 27(2):111-126.
[5]	CAO S, DU R, PENG Y, et al. Novel two stage partial denitrification (PD)-Anammox process for tertiary nitrogen removal from low carbon/nitrogen (C/N) municipal sewage[J]. Chemical Engineering Journal, 2019, 362:107-115.
[6]	吴振斌, 詹德昊, 张晟, 等.复合垂直流构建湿地的设计方法及净化效果[J]. 武汉大学学报(工学版), 2003, (1):12-16, 41.
[7]	VYMAZAL J. Constructed wetlands for treatment of industrial wastewaters:a review[J]. Ecological Engineering, 2014, 73:724-751.
[8]	HE S, WANG Y, LI C, et al. The nitrogen removal performance and microbial communities in a two-stage deep sequencing constructed wetland for advanced treatment of secondary effluent[J]. Bioresour Technol, 2018, 248(Pt B):82-88.
[9]	ZHAN X, YANG Y, CHEN F, et al. Treatment of secondary effluent by a novel tidal-integrated vertical flow constructed wetland using raw sewage as a carbon source:contribution of partial denitrification-anammox[J]. Chemical Engineering Journal, 2020, 395.
[10]	CHANG J J, LIANG K, WU S Q, et al. Comparative evaluations of organic matters and nitrogen removal capacities of integrated vertical-flow constructed wetlands:domestic and nitrified wastewater treatment[J]. J Environ Sci Health A Tox Hazard Subst Environ Eng, 2015, 50(7):757-766.
[11]	XU P, XIAO E, HE F, et al. High performance of integrated vertical-flow constructed wetland for polishing low C/N ratio river based on a pilot-scale study in Hangzhou, China[J]. Environ Sci Pollut Res Int, 2019, 26(22):22431-22449.
[12]	LIU X, WANG J, FU X, et al. Methane Emissions Driven by Adding a Gradient of Ethanol as Carbon Source in Integrated Vertical-Flow Constructed Wetlands[J]. Water, 2019, 11(5).
[13]	HU Y, HE F, WANG L, et al. The Fate of Ammonium in Integrated Vertical-flow Constructed Wetlands Using Stable Isotope Technique[J]. Polish Journal of Environmental Studies, 2016, 25(3):1027-1032.
[14]	HU Y, HE F, MA L, et al. Microbial nitrogen removal pathways in integrated vertical-flow constructed wetland systems[J]. Bioresource Technology, 2016, 207:339-345.
[15]	王亚芬, 吴振斌, 肖莹, 等. 一种模拟复合垂直流人工湿地底层厌氧环境的装置:201720195298.5[P]. 2017-11-28.
[16]	SCHUTTER M E, DICK R P. Comparison of fatty acid methyl ester (FAME) methods for characterizing microbial communities[J]. Soil Science Society of America Journal, 2000, 64(5):1659-1668.
[17]	高景峰, 彭永臻, 王淑莹, 等. 以DO, ORP, pH控制SBR法的脱氮过程[J]. 中国给水排水, 2001, 17(4):6-11.
[18]	吴振斌. 2008. 复合垂直流人工湿地[M]. 北京:科学出版社:62-63.
[19]	LI X, ZHU W, MENG G, et al. Efficiency and kinetics of conventional pollutants and tetracyclines removal in integrated vertical-flow constructed wetlands enhanced by aeration[J]. J Environ Manage, 2020, 273:111120.
[20]	王媚, 徐栋, 杜明普, 等.深床过滤用于垂直流人工湿地净化效果研究[J]. 环境科学与技术, 2017, (S2):65-70.
[21]	RAMPURIA A, GUPTA A B, BRIGHU U. Nitrogen transformation processes and mass balance in deep constructed wetlands treating sewage, exploring the anammox contribution[J]. Bioresour Technol, 2020, 314:123737.
[22]	廖晓数, 贺锋, 徐栋, 等.低C/N对湿地中硝化反硝化作用的影响[J]. 中国环境科学, 2008, 28(7):603-607.
[23]	殷士学, 陆驹飞. 硝酸异化还原成铵的微生物学过程[J]. 微生物学通报, 1997, 24(3):170-173.
[24]	ZHANG M, HUANG J C, SUN S, et al. Nitrogen removal through collaborative microbial pathways in tidal flow constructed wetlands[J]. Sci Total Environ, 2021, 758:143594.
[25]	王宇娜, 国晓春, 卢少勇, 等.人工湿地对低污染水中氮去除的研究进展:效果、机制和影响因素. 农业资源与环境学报, 2021, 38(5):722-734.
[26]	VESTAL J R, WHITE D C. Lipid analysis in microbial ecology-quantitative approaches to the study of microbial communities[J]. Bioscience, 1989, 39(8):535-541.
[27]	RAJENDRAN N, MATSUDA O, RAJENDRAN R, et al. Comparative description of microbial community structure in surface sediments of eutrophic bays[J]. Marine Pollution Bulletin, 1997, 34(1):26-33.
[28]	RAJENDRAN N, MATSUDA O, IMAMURA N, et al. Variation in microbial biomass and community structure in sediments of eutrophic bays as determined by phospholipid ester-linked fatty-acids[J]. Applied and Environmental Microbiology, 1992, 58(2):562-571.