初始碱度和温度对养猪废水厌氧发酵过程的影响特征

廖杰; 谢威; 刘超翔; 范洪勇

doi:10.13205/j.hjgc.202302008

初始碱度和温度对养猪废水厌氧发酵过程的影响特征

doi: 10.13205/j.hjgc.202302008

廖杰¹,
谢威¹,
刘超翔^2, ,,
范洪勇³

1. 厦门理工学院环境科学与工程学院, 厦门市水资源利用与保护重点实验室, 福建厦门 361024;
2. 福州大学环境与安全工程学院, 福州 350108;
3. 青岛理工大学环境与市政工程学院, 山东青岛 266033

基金项目:

国家自然科学基金项目(51878582)

福建省STS计划项目(2019T3023)

详细信息

作者简介:
廖杰(1983-),女,实验师,主要研究方向为污水生态处理与回用。liaoj@xmut.edu.cn

通讯作者:
刘超翔(1974-),男,研究员,主要研究方向为污水生态处理与回用。liucx@fzu.edu.cn

计量
- 文章访问数: 134
- HTML全文浏览量: 13
- PDF下载量: 6
- 被引次数: 0
出版历程
- 收稿日期: 2022-06-16
- 网络出版日期: 2023-05-25
- 刊出日期: 2023-02-01

EFFECTS OF INITIAL ALKALINITY AND TEMPERATURE ON ANAEROBIC FERMENTATION PROCESS OF PIGGERY WASTEWATER

1. The Key Laboratory of Water Resources Utilization and Protection of Xiamen, School of Environmental Science and Engineering, Xiamen University of Technology, Xiamen 361024, China;
2. School of Environmental and Safety Engineering, Fuzhou University, Fuzhou 350108, China;
3. School of Environmental and Municipal Engineering, Qingdao University of Technology, Qingdao 266033, China

摘要

摘要: 采用序批式实验研究了不同初始碱度(3503,5500,7500 mg/L,以CaCO₃计)和温度(20℃、35℃和50℃)下养猪废水的厌氧发酵过程,考察其对发酵液pH、挥发性脂肪酸、产气量、沼液养分、重金属含量、抗生素和抗性基因的影响特征。结果表明:初始碱度调控会延缓水解酸化阶段的启动,强化产酸过程,提高总产酸量;高碱度对发酵液pH的维持能力最高;初始碱度调控适用于以产酸为目的的中温(35℃)和高温(50℃)厌氧发酵。35℃和50℃有利于沼液中养分的释放,50℃时养分浓度最高,为(1365.14±124.38)~(1471.71±135.29)mg/L。50℃厌氧发酵更有利于沼液中水溶态重金属(Cu、Zn)的削减,消减比例分别为(81.53±9.51)~(86.04±7.72)%和(96.48±8.73)~(97.81±10.29)%。厌氧发酵对沼液中抗生素(土霉素和诺氟沙星)具有削减作用,50℃时的削减比例最高,比20℃和35℃分别高(14.61±1.39)~(56.26±5.24)%和(23.83±3.21)~(85.84±17.35)%。50℃和适量初始碱度调控能够降低抗性基因的相对丰度。该研究结果可为畜禽养殖废水厌氧发酵的工艺优化提供参考。
- 碱度 /
- 温度 /
- 厌氧发酵 /
- 养猪废水 /
- 抗生素
Abstract: The anaerobic fermentation process of piggery wastewater at different initial alkalinity levels (3503, 5500, 7500 mg/L, as CaCO₃) and temperature (20, 35, 50℃) was studied by sequential batch experiment. The characteristics of its influences on pH, volatile fatty acids, gas production, biogas slurry nutrients, heavy metal content, antibiotics and resistance genes were investigated. The results revealed that the initial alkalinity adjustment could delay the initiation of the hydrolysis and acidification stage of anaerobic fermentation, strengthen the process of acid production and increase total acid production. The highest pH maintenance ability was presented with the operation of the highest level of alkalinity. The initial alkalinity regulation was suitable for anaerobic fermentation at moderate and high temperature with the purpose of producing acid. 35℃ and 50℃ was conducive to releasing nutrients from fermentation substrates, and the highest nutrient concentration was (1365.14±124.38)~(1471.71±135.29) mg/L at 50℃. Anaerobic fermentation at 50℃ was more conducive to the reduction of soluble heavy metals (Cu,Zn) in biogas slurry, and the reduction ratios were (81.53±9.51)~(86.04±7.72)% and (96.48±8.73)~(97.81±10.29)%, respectively. Anaerobic fermentation could reduce the concentrations of antibiotics (oxytetracycline and norfloxacin) in biogas slurry, and the highest reduction rate was (14.61±1.39)~(56.26±5.24)% and (23.83±3.21)~(85.84±17.35)% at 50℃ compared with 20℃ and 35℃, respectively. The relative abundance of resistance genes were decreased at 50℃ and appropriate initial alkalinity regulation effectively. The results of this research could provide a reference for the optimization of the anaerobic fermentation process of livestock wastewater.
- alkalinity /
- temperature /
- anaerobic fermentation /
- piggery wastewater /
- antibiotic

HTML全文

参考文献(37)

[1]	范洪勇,张婷凤,廖杰,等. 畜禽固液粪污好氧-异位协同消纳技术研究[J]. 环境工程,2018,36(7):102-106.
[2]	FAN H Y, LIAO J, ABASS O K, et al. Effects of compost characteristics on nutrient retention and simultaneous pollutant immobilization and degradation during co-composting process[J]. Bioresource Technology, 2019, 275:61-69.
[3]	李敏,陈滢,刘敏,等. 畜禽养殖废水沼液生物脱氮的影响因素研究[J]. 环境工程,2015,33(4):20-24.
[4]	ALVAREZ R, VILLCA S, LIDEN G. Biogas production from llama and cow manure at high altitude[J]. Biomass and Bioenergy, 2006,30(1):66-75.
[5]	ULUDAG-DEMIRER S, DEMIRE G N, FREAR C, et al. Anaerobic digestion of dairy manure with enhanced ammonia removal[J].Journal of Environmental Management,2008,86(1):193-200.
[6]	蒋玲燕.污水处理厂污泥厌氧消化优化设计与运行探讨[J].给水排水,2015,51(2):32-35.
[7]	祁国平,李维维,牛永健,等.碱度NaOH投加量对土霉素菌渣减量化试验研究[J].环境工程,2020,38(11):140-144 ,151.
[8]	赵明明,李夕耀,李璐凯,等. 碱度类型及浓度对剩余污泥中温厌氧消化的影响[J].中国环境科学,2019,5(5):1954-1960.
[9]	张玉鹏.碱度对甲烷发酵系统主要功能菌群代谢的影响与机制[D].哈尔滨:哈尔滨工业大学,2014.
[10]	胡贵川.厌氧发酵技术处理畜禽养殖废水的研究进展[J].贵州农业科学,2021,49(7):67-74.
[11]	KIN M,AHN Y H,SPEECE R E. Comparative process stability and efficiency of anaerobic digestion; mesophilie vs. thermophilic[J].Water Research,2002,36(17):4369-4385.
[12]	何顺,李忠棠,谭松凡,等.污泥协同香蕉秸秆中温厌氧发酵中pH对产酸的影响[J]. 环境工程,2019,37(2):153-157.
[13]	张彤,翟宁宁,王晓娇,等.初始pH值和物料配比对高温混料厌氧发酵进程的影响[J].环境科学学报,2016, 36(7):2571-2579.
[14]	SUNG S,LIU T. Ammonia inhibition on thermophilic anaerobic digestion[J].Chemosphere,2003,53(1):43-52.
[15]	刘艳军,张月强,于成鹏,等.畜禽粪便厌氧发酵产气特性研究[J].现代农业科技,2021(18):174-176,179.
[16]	关正军,李文哲,郑国香,等.牛粪固液分离液两相厌氧发酵技术[J].农业工程学报,2011,27(7):300-305.
[17]	徐玉璐,乔子茹,储思琴,等.污泥资源化过程中新兴污染物的赋存与控制研究进展[J].环境工程,2021,39(9):146-153.
[18]	国家环境保护总局.水和废水监测分析方法[M].4版.北京:中国环境科学出版社,2002.
[19]	DARJA P, FRANC P, ANDREJA G. Kinetics of methane production during anaerobic fermentation of chicken manure with sawdust and fungi pre-treated wheat straw[J]. Waste Management, 2020, 102:170-178.
[20]	卢振威,孔德望,张克强, 等.不同温度下猪粪厌氧发酵的氨胁迫效应[J].环境工程学报,2021, 15(10):3297-3305.
[21]	CHEN Z M, WAND Q, MA J W, et al. Fungal community composition change and heavy metal accumulation in response to the long-term application of anaerobically digested slurry in a paddy soil[J]. Ecotoxicology and Environmental Safety, 2020, 96:110453.
[22]	YUAN Y Y, HU X Y, CHEN H B, et al. Advances in enhanced volatile fatty acid production from anaerobic fermentation of waste activated sludge[J]. Science of the Total Environment, 2019, 694:133741.
[23]	宫亚斌, 姚建刚, 谭婧. 餐厨垃圾中温与中高温过渡区厌氧产沼效率研究[J]. 环境工程, 2022, 40(3):132-138.
[24]	CHEN H B, CHANG S. Dissecting methanogenesis for temperature-phased anaerobic digestion:impact of temperature on community structure, correlation, and fate of methanogens[J]. Bioresource Technology, 2020, 306:123104.
[25]	KOVALOVSZKIA A, TREUA L, ELLEGAARD L, et al. Modeling temperature response in bioenergy production:novel solution to a common challenge of anaerobic digestion[J]. Applied Energy, 2020, 263:114646.
[26]	吴美容,张瑞,周俊,等.温度对产甲烷菌代谢途径和优势菌群结构的影响[J]. 化工学报,2014,65(5):1602-1605.
[27]	孟晓山,张玉秀,隋倩雯,等.氨氮浓度对猪粪厌氧消化及产甲烷菌群结构的影响[J].环境工程学报,2018,12(8):2346-2356.
[28]	魏勃. 氨态氮对产甲烷菌的抑制及有机负荷提高引发的厌氧反应器微生物群落演替[D].西安:西安建筑科技大学,2016.
[29]	ZHENG X R, LIU Y Q, HUANG J M, et al. The influence of variables on the bioavailability of heavy metals during the anaerobic digestion of swine manure[J]. Ecotoxicology and Environmental Safety, 2020, 195:110457.
[30]	KOKI M, DAI H, SAKAE T, et al. Microbiology of nitrogen cycle in animal manure compost[J]. Microbial Biotechnology, 2011, 4(6):700-709.
[31]	李轶,宫兴隆,郭敬阳,等.不同预处理玉米秸秆对猪粪厌氧发酵重金属镉钝化效果[J].农业工程学报,2020,36(11):254-260.
[32]	周阳思雨.重金属形态在生物质厌氧发酵体系中分布于转化研究[D].厦门:厦门大学,2017.
[33]	CHENG D L, NGO H H,GUO W S, et al. Removal process of antibiotics during anaerobic treatment of swine wastewater[J]. Bioresource Technology, 2020,300:122707.
[34]	强虹,杨祎楠,李娜,等.金霉素浓度对鸡粪中温厌氧消化特性及抗生素降解的影响[J].农业工程学报,2019,35(10):181-190.
[35]	LI C X, ZHANG G Y, ZHANG Z K, et al. Hydrothermal pretreatment for biogas production from anaerobic digestion of antibiotic mycelial residue[J].Chemical Engineering Journal,2015,279:530-537.
[36]	袁学华. 畜禽粪便好氧堆肥和厌氧发酵过程中典型抗生素消减研究进展[J].池州学院学报,2021,35(6):32-36.
[37]	BIYENSA G, ESTER F P, STEFANIA C, et al. Manure anaerobic digestion effects and the role of pre-and post-treatments on veterinary antibiotics and antibiotic resistance genes removal efficiency[J]. Science of the Total Environment, 2020, 721:137532.